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RISS 인기검색어
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- 저자 : Jinyun Ji (검색결과 4 건)
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Yunhua Zhang,Jinyun Ji,Xiao Zhao,Ruoheng Jin,Jian Hong Li,Hu Wan 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.4
A previous study demonstrated that a β-N-acetyl-D-hexosaminidase (NlHex4) from the brown planthopper could serve as a new potential target for biopesticides due to its requirement for molting, and lethal phenotypes were observed via RNA interference. However, the enzymatic properties of NlHex4 remain unclear. In the present study, NlHex4 was expressed, purified and characterized. Recombinant NlHex4 was functionally expressed in baculovirus-infected insect cells and purified with a molecular weight of approximately 70 kDa. Recombinant NlHex4 had an optimal pH and temperature of 6.0 and 60 °C, respectively. Moreover, recombinant NlHex4 exhibited thermal stability at 50 °C for at least 1 h. Furthermore, the activity of recombinant NlHex4 was inhibited by the metal ions Fe2+ and Fe3+ and the serine protease inhibitor phenylmethanesulfonyl fluoride. These results reveal the enzymatic properties of NlHex4 and provide a basis for future investigation of the interaction between HEX and chitin oligosaccharide.
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Yashu Zhang,Xiao Zhao,Jinyun Ji,Tinghao Kang,Jian Hong Li,Hu Wan 한국응용곤충학회 2016 Journal of Asia-Pacific Entomology Vol.19 No.2
In the present study, a phenylalanine hydroxylase (PAH) was cloned and characterized from the common cutworm Spodoptera litura (SlPAH). The SlPAH gene is an 1863 bp full-length cDNAwith a 1371 bp open reading frame that encodes a predicted 52.35 kDa protein of 457 amino acid residueswith an isoelectric point of 5.39. The SlPAH gene contains 6 exons and 5 introns. A multiple sequence analysis of SlPAH showed an 88–68% sequence identity to Papilio xuthus PAH and other insect PAHs. A phylogenetic tree revealed that SlPAH is a novel member of the PAH family. Tissue distribution analyses indicated that SlPAH is constitutively expressed in the epidermis, midgut, and fat body, with the highest expression occurring in the fat body on days 2 and 3 of the sixth-instar larval stage and day 1 of the prepupa stage. However, RNAi-mediated SlPAH gene silencing had no observable phenotype in S. litura larvae. Additionally, SlPAH expression is acutely up-regulated in the fat body after injection with Bacillus thuringiensis, Escherichia coli, or Beauveria bassiana. Therefore, these results provide a molecular basis for the functional roles of SlPAH during development and the innate immune response in S. litura.
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Duo Shen,Hongyu Zhao,Peng Zeng,Jinyun Song,Yiqiong Yang,Xuefeng Gu,Qinghua Ji,Wei Zhao 대한위암학회 2020 Journal of gastric cancer Vol.20 No.3
Purpose: Circular RNAs (circRNAs) are a new class of RNA molecules whose function is largely unknown. There is a growing evidence that circRNAs play an important regulatory role in the progression of a variety of human cancers. However, the exact roles and the mechanisms of circRNAs in gastric cancer are not clear. In this study, we aimed to elucidate the mechanism of hsa_circ_0005556. Materials and Methods: Real-time quantitative polymerase chain reaction was used to detect the expression of hsa_circ_0005556, miR-4270, and matrix metalloproteinase-19 (MMP19) in gastric cancer tissues and cell lines. The expression of hsa_circ_0005556 in gastric cancer cells was silenced by lentivirus, and cell proliferation, invasion, migration, and tumorigenesis in nude mice were assessed to evaluate the function of hsa_circ_0005556 in gastric cancer. Results: The expression of hsa_circ_0005556 in gastric cancer tissues and gastric cancer cell lines was higher compared to normal controls. In vitro, the downregulation of hsa_circ_0005556 significantly inhibited proliferation, migration, and invasion of gastric cancer cells. In vivo, the downregulation of hsa_circ_0005556 suppressed tumor growth in nude mice. Conclusions: Our study shows that the hsa_circ_0005556/miR-4270/MMP19 axis is involved in proliferation, migration, and invasion of gastric cancer cells through the competing endogenous RNA (ceRNA) mechanism.
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Hu Wan,Yashu Zhang,Xiao Zhao,Hong You,Jian Hong Li,Jinyun Ji 한국응용곤충학회 2015 Journal of Asia-Pacific Entomology Vol.18 No.3
Insect cytokine growth-blocking peptides (GBPs) play functional roles in growth regulation and the innate immune response.Moreover, high concentrations of GBPs significantly induce larvalmorphological abnormalities and lethality. In the present study, a recombinant AcNPV, AcNPV-SeGBPm, expressing a growth-blocking peptide fromthe beet armywormSpodoptera exigua (SeGBP) under the control of a polyhedrin promoterwas constructed. Western blot analysis indicated that the mature peptide of SeGBP (SeGBPm) is expressed as a 3-kDa peptide in recombinant baculovirus-infected Sf9 insect cells. SeGBPm expression in Sf9 insect cells decreased cell viability significantly and induced apoptotic cell death. In addition, the body weights of S. exigua larvae inoculated with AcNPV-SeGBPm decreased significantly compared to those of larvae inoculated with wild-type AcNPV. Moreover, AcNPV-SeGBPm improved the rate of larvae killing (LT50) by approximately 1 day compared to wild-type AcNPV. Taken together, the obtained data demonstrate the great potential for future investigations of growth-blocking peptides for use as biopesticides.
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