Serum cardiac troponin I concentrations in clinically normal and Dirofilaria immitis infected Korean Jindo dogs

Hwang, Soo-Hyen,Park, Hyung-Jin,Seo, Kyoung-Won,Lee, Sang-Eun,Song, Kun-Ho The Korean Society of Veterinary Science 2014 大韓獸醫學會誌 Vol.54 No.1

This study was conducted to determine the serum cardiac troponin I (cTnI) concentrations in clinically normal (uninfected) Korean Jindo dogs and those infected with Dirofilaria (D.) immitis. Forty Korean Jindo dogs (22 females, 18 males) were obtained from two kennels in the Boryeong area of Chungnam Province and the Daejeon area, Korea. Significantly higher cTnI concentrations were observed in D. immitis-infected dogs than clinically normal dogs. cTnI testing in addition to thoracic radiography, echocardiography, and electrocardiography shows promise as an additional method for diagnosis of heartworm infection.

Levels of Tobacco-specific Metabolites among Non-smoking Lung Cancer Cases at Diagnosis: Case-control Findings

Hwang, Sang-Hyun,Ryu, Hye-Jung,Kang, Soo Jin,Yun, E. Hwa,Lim, Min Kyung,Kim, Heung Tae,Lee, Jin Soo,Lee, Do-Hoon Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.11

Background: Environmental tobacco smoking (ETS) significantly contributes to morbidity and mortality and is a known risk factor for lung cancer development in lifelong nonsmokers. The metabolite 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL) and its glucuronides (NNAL-Glucs) have now emerged as leading biomarkers for the study of carcinogen exposure in non-smokers exposed to ETS. Materials and Methods: We carried out our study on NNAL in the urine of non-smokers exposed to ETS and the association between ETS and lung cancer. Subjects were enrolled from 2008-2010. NNAL was analyzed for 74 non-smoking lung cancer and 85 healthy controls. The main objective of this study was to provide an estimate of the risk of lung cancer from exposure to ETS in the Korean population. Results: The mean NNAL concentration in urine was significantly lower in non-smoking patient groups (n=74) than in control groups (n=85) ($4.7{\pm}15.0$ pg/mg, $6.5{\pm}17.9$ pg/mg, respectively, Mann-Whitney U test, p<0.001). Conclusions: The urine NNAL of non-smoking patients with lung cancer was not elevated with regard to the non-smoking control group. This may be due to life-style changes after diagnosis. A prospective study will be needed to evaluate the association of NNAL and non-smoking lung cancer.

Effects of Fatty Acids and Vitamin E Supplementation on Behavioral Development of the Second Generation Rat

Hwang, Hye-Jin,Um, Young-Sook,Chung, Eun-Jung,Kim, Soo-Yeon,Park, Jung-Hwa,Lee, Yang-Cha-Kim The Korean Society of Food Science and Nutrition 2002 Preventive Nutrition and Food Science Vol.7 No.3

In this study, we examined the effects of dietary fatty acids on the fatty acid composition of phospholipid fractions in regions of the brain and on behavioral development in rats. The Sprague Dawley rats were fed the experimental diets 3~4 wks prior to the conception. Experimental diets consisted of 10% fat(wt/wt) which were from either safflower oil (SO, poor in $\omega$3 fatty acids), mixed oil MO, P/M/S ratio : 1:1.4:1, $\omega$6/$\omega$3 ratio = 6.3), or mixed oil supplemented with vitamin E (+500 mg/kg diet). At 3 and 9 weeks of age, frontal cortex (FC), corpus striatum (CS), hippocampus (H), and cerebellum (CB) were dissected from the whole brain. The fatty acid content was determined in the different phospholipid fractions: phosphatidylcholine (PC), phosphatidyl-serine (PS), and phosphatidylethanolamine (PE) in the rat brain regions. In the visual discrimination test, the order of the cumulative errors made in Y-water maze test were SO > MO > ME. This suggested that the balanced diet supplemented with vitamin I had the most beneficial effect on learning ability. The overall characteristics of correlation between fatty acids and behavior development were that the frequency of cumulative errors were negatively correlated significantly with monounsaturated fatty acids (MUFAs), ie., 18:1 $\omega$9 and 22:1 $\omega$9. Docosa-hexaenoic acid (22:6 $\omega$3) of PS in frontal cortex (FC) was negatively correlated with the number of errors made in the Y-water maze test.22:5 $\omega$6 PS in hippocampus (H), PC and PE in corpus striatum (CS), PC in cerebellum (CB) were positively correlated with cumulative errors. And these errors were negatively correlated with 20:4 $\omega$ 6 of PE in corpus striatum (CS) and PC in cerebellum (CB). Especially, O1eic acid (18:1 u 9) in all phospholipid fractions (PC, PS, PE) of hippocampus was negatively correlated with the number of errors. These findings demonstrate that the MUFAs were might be essential for proper brain development, especially in hippocampus which is generally thought to be the regions of memory and learning.

A novel histone deacetylase inhibitor, CG0006, induces cell death through both extrinsic and intrinsic apoptotic pathways

Hwang, Jung Jin,Kim, Yong Sook,Kim, Mi Joung,Jang, Sejin,Lee, Je-Hwan,Choi, Jene,Ro, Seonggu,Hyun, Young-Lan,Lee, Jung Shin,Kim, Choung-Soo Lippincott Williams Wilkins, Inc. 2009 ANTICANCER DRUGS Vol.20 No.9

Histone deacetylase inhibitors (HDACIs) are potent anticancer drugs, and suberoylanilide hydroxamic acid is used for the treatment of cutaneous T-cell lymphoma patients. We synthesized a novel hydroxamate-based HDACI, CG0006, and assessed its antiproliferative effects on the NCI-60 cancer cell panel and cell lines from liver and stomach cancers that are common in Korea. Micromolar levels of CG0006 induced cell death in several breast, central nervous system, colon, hematopoietic, lung, melanoma, ovarian, prostatic, renal, and stomach cancer cell lines. We further analyzed cell death mechanisms activated by CG0006 in HCT116 (colon cancer) and K562 (leukemia) cells. First, to test the activity of CG0006, we analyzed acetylation of substrates of HDACs and effect on gene expression. CG0006 increased acetylation of histone 3, histone 4, and tubulin in a time-dependent and dose-dependent manner in both HCT116 and K562 cells. Moreover, CG0006 increased the mRNA level of p21 and decreased that of Bcl-xl efficiently in HCT116 cells. Cell cycle analysis showed G2-M arrest, and increased apoptosis in populations of HCT116 and K562 cells treated with CG0006. Western blot analysis showed that CG0006 increased levels of p21 in HCT116 cells and of p21 and p27 in K562 cells. In addition, CG0006 activated caspase-9, caspase-3, and caspase-8. These results indicate that CG0006 induces death in HCT116 and K562 cells through both intrinsic and extrinsic apoptotic pathways. The HDACI CG0006 may be a potent anticancer drug for solid tumors and leukemia.

Study of Molecular Reorientation in Liquid with Raman Spectroscopy. Intermolecular Interaction of Hexafluorobenzene with Benzene

Hwang, Hyun-Jin,Kim, Kwan,Kim, Myung-Soo Korean Chemical Society 1984 Bulletin of the Korean Chemical Society Vol.5 No.6

Raman spectroscopy was used to study the reorientational motion of hexafluorobenzene in benzene and also in neutral solvents. From the viscosity dependence of the reorientation time, intermolecular interaction in the systems was investigated. No evidence was found to support the presence of long-lived 1:1 complex of hexafluorobenzene and benzene. The unusual viscosity dependence of the reorientational motion observed in the systems was explained as due to the formation of transient complexes.

Dry etching of polydimethylsiloxane using microwave plasma

Hwang, Sung Jin,Oh, Dong Joon,Jung, Phill Gu,Lee, Sang Min,Go, Jeung Sang,Kim, Joon-Ho,Hwang, Kyu-Youn,Ko, Jong Soo IOP 2009 JOURNAL OF MICROMECHANICS AND MICROENGINEERING - Vol.19 No.9

<P>This paper presents a new polydimethylsiloxane (PDMS) dry-etching method that uses microwave plasma. The applicability of the method for fabricating microstructures and removing residual PDMS is also verified. The etch rate of PDMS was dominantly influenced by the gas flux ratio of CF<SUB>4</SUB>/O<SUB>2</SUB> and the microwave power. While the PDMS etch rate increased as the flux ratio of CF<SUB>4</SUB> was increased, the etch rate decreased as the flux ratio of O<SUB>2</SUB> was increased. The maximum etch rate of 4.31 µm min<SUP>−1</SUP> was achieved when mixing oxygen (O<SUB>2</SUB>) and tetrafluoromethane (CF<SUB>4</SUB>) at a 1:2 ratio at 800 W power. The PDMS etch rate almost linearly increased with the microwave power. The ratio of the vertical etch rate to the lateral etch rate was in a range of 1.14–1.64 and varied with the gas fluxes. In consideration of potential applications of the proposed PDMS etching method, array-type PDMS microwells and network-type microprotrusion structures were fabricated. The contact angle was dramatically increased from 104° (non-etched PDMS surface) to 148° (etched PDMS surface) and the surface was thereby modified to be superhydrophobic. In addition, a thin PDMS skin that blocked holes and PDMS residues affixed in nickel microstructures was successively removed.</P>

Multiple Target Angle-tracking Using Angular Innovations Extracted from Noise Subspace

Hwang Soo Bok,Kim Jin Seok,Kim Hyun Sik,Nam Ki Gon The Acoustical Society of Korea 2005 韓國音響學會誌 Vol.24 No.e1

Ryu et al. proposed a multiple target angle-tracking algorithm without a data association problem using angular innovations. This algorithm, however, needs the computational loads in proportion to the square number of sensors regardless of the number of targets, because it uses a nonlinear equation between the signal subspace and angular innovation. In this Paper, we proposed an efficient algorithm for the multiple target angle-tracking using angular innovations. The proposed algorithm extracts the angular innovations from noise subspace. Also, it is demonstrated by computer simulations dealing with the tracking of crossing targets. The simulation results show that the computational loads of the proposed algorithm are $80\%$ and $60\%$ of those of Ryu's algorithm for 3 targets and 6 targets without degrading the performance of the target tracking.

ROLE OF GRAIN BOUNDARY CARBIDES IN CRACKING BEHAVIOR OF Ni BASE ALLOYS

Hwang, Seong Sik,Lim, Yun Soo,Kim, Sung Woo,Kim, Dong Jin,Kim, Hong Pyo Korean Nuclear Society 2013 Nuclear Engineering and Technology Vol.45 No.1

The primary water stress corrosion cracking (PWSCC) of Alloy 600 in a PWR has been reported in the control rod drive mechanism (CRDM), pressurizer instrumentation, and the pressurizer heater sleeves. Recently, two cases of boric acid precipitation that indicated leaking of the primary cooling water were reported on the bottom head surface of steam generators (SG) in Korea. The PWSCC resistance of Ni base alloys which have intergranular carbides is higher than those which have intragranular carbides. Conversely, in oxidized acidic solutions like sodium sulfate or sodium tetrathionate solutions, the Ni base alloys with a lot of carbides at the grain boundaries and shows less stress corrosion cracking (SCC) resistance. The role of grain boundary carbides in SCC behavior of Ni base alloys was evaluated and effect of intergranular carbides on the SCC susceptibility were reviewed from the literature.

Identification and characterization of a serine protease inhibitor of Paragonimus westermani

Hwang, Jin-Hee,Lee, Wook-Gyo,Na, Byoung-Kuk,Lee, Hyeong-Woo,Cho, Shin-Hyeong,Kim, Tong-Soo Springer-Verlag 2009 Parasitology research Vol.104 No.3

Pretreatment with paricalcitol attenuates inflammation in ischemia–reperfusion injury via the up-regulation of cyclooxygenase-2 and prostaglandin E2

Hwang, Hyeon Seok,Yang, Keum Jin,Park, Ki Cheol,Choi, Hyun Soo,Kim, So Hee,Hong, Sung Youp,Jeon, Byeong Hwa,Chang, Yoon Kyung,Park, Cheol Whee,Kim, Suk Young,Lee, Sang Ju,Yang, Chul Woo Oxford University Press 2013 Nephrology, dialysis, transplantation Vol.28 No.5

<P><B>Background</B></P><P>The effect of paricalcitol on renal ischemia–reperfusion injury (IRI) has not been investigated. We examined whether paricalcitol is effective in preventing inflammation in a mouse model of IRI, and evaluated the cyclooxygenase-2 (COX-2) and prostaglandin E2 (PGE2) pathways as a protective mechanism of paricalcitol.</P><P><B>Methods</B></P><P>Paricalcitol (0.3 μg/kg) was administered to male C57BL/6 mice 24 h before IRI. Bilateral kidneys were subjected to 23 min of ischemia, and mice were killed 72 h after IRI. The effects of paricalcitol on renal IRI were evaluated in terms of renal function, tubular necrosis, apoptotic cell death, inflammatory cell infiltration and inflammatory cytokines. The effects of paricalcitol on COX-2, PGE2 and its receptors were investigated.</P><P><B>Results</B></P><P>Paricalcitol pretreatment improved renal function (decreased blood urea nitrogen and serum creatinine levels), tubular necrosis and apoptotic cell death in IRI-mice kidneys. The infiltration of inflammatory cells (T cells and macrophages), and the production of proinflammatory cytokines (RANTES, tumor necrosis factor-α, interleukin-1β and interferon-γ) were reduced in paricalcitol-treated mice with IRI. Paricalcitol up-regulated COX-2 expression, PGE2 synthesis and mRNA expression of receptor subtype EP4 in post-ischemic renal tissue. The cotreatment of a selective COX-2 inhibitor with paricalcitol restored functional injury and tubular necrosis in paricalcitol-treated mice with IRI.</P><P><B>Conclusions</B></P><P>Our study demonstrates that paricalcitol pretreatment prevents renal IRI via the inhibition of renal inflammation, and the up-regulation of COX-2 and PGE2 is one of the protective mechanisms of paricalcitol in renal IRI.</P>