Delineating the dynamic evolution from preneoplasia to invasive lung adenocarcinoma by integrating single-cell RNA sequencing and spatial transcriptomics

Zhu Jianfei,Fan Yue,Xiong Yanlu,Wang Wenchen,Chen Jiakuan,Xia Yanmin,Lei Jie,Gong Li,Sun Shiquan,Jiang Tao 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

The cell ecology and spatial niche implicated in the dynamic and sequential process of lung adenocarcinoma (LUAD) from adenocarcinoma in situ (AIS) to minimally invasive adenocarcinoma (MIA) and subsequent invasive adenocarcinoma (IAC) have not yet been elucidated. Here, we performed an integrative analysis of single-cell RNA sequencing (scRNA-seq) and spatial transcriptomics (ST) to characterize the cell atlas of the invasion trajectory of LUAD. We found that the UBE2C + cancer cell subpopulation constantly increased during the invasive process of LUAD with remarkable elevation in IAC, and its spatial distribution was in the peripheral cancer region of the IAC, representing a more malignant phenotype. Furthermore, analysis of the TME cell type subpopulation showed a constant decrease in mast cells, monocytes, and lymphatic endothelial cells, which were implicated in the whole process of invasive LUAD, accompanied by an increase in NK cells and MALT B cells from AIS to MIA and an increase in Tregs and secretory B cells from MIA to IAC. Notably, for AIS, cancer cells, NK cells, and mast cells were colocalized in the cancer region; however, for IAC, Tregs colocalized with cancer cells. Finally, communication and interaction between cancer cells and TME cell-induced constitutive activation of TGF-β signaling were involved in the invasion of IAC. Therefore, our results reveal the specific cellular information and spatial architecture of cancer cells and TME subpopulations, as well as the cellular interaction between them, which will facilitate the identification and development of precision medicine in the invasive process of LUAD from AIS to IAC.

Characterization and antioxidant properties of chitosan/ethyl-vanillin edible films produced via Schiff-base reaction

Jianfei Zhu,Xiaomei Chen,Tingting Huang,Dongling Tian,Ruiping Gao 한국식품과학회 2023 Food Science and Biotechnology Vol.32 No.2

In this paper, chitosan/ethyl-vanillin (CS-EV) Schiff-base edible films with CS and EV at different concentrations and ratios were successfully prepared. The optical barrier properties, water contact angle, mechanical performance, water vapor transmission, antioxidant properties, thermal properties, and morphological structure of the films were compared. The results suggested that the tensile strength (TS) attained a maximum value of 64.63 MPa at a concentration of 4% EV. Moreover, water diffusion was prevented through the compact structure of the CS-EV edible film. Additionally, the two sides of the CS-EV film show different textures due to their different hydrophilicity/hydrophobicity. In particular, the films of CS possessed superior thermal stability, while those of CS-EV exhibited higher antioxidant activity.

Optimization of Ultrasound-assisted Extraction Process of Perilla Seed Meal Proteins

Jianfei Zhu,Qiao Fu 한국식품과학회 2012 Food Science and Biotechnology Vol.21 No.6

The quantitative effects of ultrasonic power,extraction time, ratio of liquid-solid, and extraction temperature on yield of perilla seed meal proteins were investigated using response surface methodology (RSM). The experimental data obtained were fitted to a secondorder polynomial equation using multiple regression analysis and also analyzed by appropriate statistical methods. By solving the regression equation and also by analyzing the response surface contour plots, the optimal proteins extraction conditions were determined. According to the experimental results, the optimal extraction process of perilla meal proteins was that extraction temperature of 40oC, power of 61 W, extraction time of 12 min, and ratio of liquid to solid of 40 mL/g. Under these conditions, the extracted contents of the perilla meal proteins was 10.77%.

Preparation and characterization of vanillin-conjugated chitosan-stabilized emulsions via a Schiff-base reaction

Jianfei Zhu,Tingting Huang,Xiaomei Chen,Dongling Tian,Lei Wang,Ruiping Gao 한국식품과학회 2023 Food Science and Biotechnology Vol.32 No.11

In the current work, vanillin-conjugated chitosan stabilized emulsions (CSVAEs) were successfully prepared and its characterization and antibacterial properties were investigated. Under stirring condition, CSVAEs were produced by a Schiff base reaction between the vanillin aldehyde group and the chitosan active amino group. The CSVAEs were described through Fourier transform infrared spectroscopy, X-ray diffraction, ultraviolet spectrophotometry and thermogravimetric analysis, which demonstrated the generation of Schiff bases between vanillin and chitosan. Furthermore, the CSVAEs displayed differences at different pH values, indicating their potential as pH-responsive materials. By studying their release behavior, pH 4 was a critical point at which the properties of the CSVAEs changed. The antibacterial tests showed that the CSVAEs had good pH-responsive antibacterial abilities against Staphylococcus aureus and Escherichia coli.

Effect of defect types in monolayer MoS2 on SO2 adsorption

Liu Ge,Liu Jianfei,Yan Jun,Chen Yunlin,Zhu Yabin,Tian Yahui 한국물리학회 2022 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.81 No.5

As a two-dimensional semiconductor material, molybdenum disulfde (MoS2) is extensively applied to sulfur dioxide (SO2) gas sensor. However, point defects will inevitably be produced in the preparation of MoS2, which signifcantly afect the adsorption properties of MoS2. Thus, understanding the properties of diferent point defects is essential for the successful application of MoS2. In this work, four types of point defects (VS-MoS2, VS2-MoS2, VMoS3-MoS2 and VMoS6-MoS2) of monolayers MoS2 were proposed, and the adsorption characteristics of SO2 on four typical defective MoS2 monolayers were investigated based on frst principles. The adsorption models were established to calculate the electronic properties and adsorption parameters of the adsorption systems. The results show that VMoS6-MoS2 defect has excellent adsorption properties for SO2 due to its largest adsorption energy, charge transfer and electrical response. The obtained results will contribute to the understanding of the efects on the MoS2 monolayers with diferent defects for SO2 sensing application.

Study on liquid-solid jet erosion characteristics of 316L stainless steel

Guan Wang,Qianfeng Gao,Linyuan Kou,Pei Zhang,Wenhui Wang,Jianfei Deng,Xuejun Zhu 대한기계학회 2023 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.37 No.4

The essence of erosion is the dynamic damage and material loss process of a material caused by particle impact. The failure mechanism of erosion is the result of the interaction of multiphase flow, particle characteristics, material properties, particle impact process, and other factors. This paper employs experimental and numerical simulation methods to investigate the erosion behavior of a solid-liquid two-phase flow of 316L stainless steel jet from the angle of erosion, to explain the erosion behavior from both macroscopic and microscopic perspectives. The results discovered that the kinetic energy of the fluid is converted into pressure potential energy, which changes the kinematic characteristics of the particles and influences how they erode. The particles erode the target material by plowing and impacting at various erosion angles, and the erosion rate exhibits an increasing-decreasing-increasing tendency as the erosion angle increases, the 45° corresponds to the maximum erosion rate. Due to the particles to harden the target surface, the erosion effect is diminished in the time dimension. Comparing to high erosion angles, the reduction rate of the erosion rate in the late experiment stage is small for slow erosion angles. In the last 3 hours of the experiment, the total erosion of 316L stainless steel at 90° erosion angle was only 35 %. This provides a theoretical foundation for failure prevention in transport components containing solid particles.

Protein Microarray Characterization of the <i>S</i> -Nitrosoproteome

Lee, Yun-Il,Giovinazzo, Daniel,Kang, Ho Chul,Lee, Yunjong,Jeong, Jun Seop,Doulias, Paschalis-Thomas,Xie, Zhi,Hu, Jianfei,Ghasemi, Mehdi,Ischiropoulos, Harry,Qian, Jiang,Zhu, Heng,Blackshaw, Seth,Dawso The American Society for Biochemistry and Molecula 2014 Molecular and Cellular Proteomics Vol.13 No.1

<P>Nitric oxide (NO) mediates a substantial part of its physiologic functions via <I>S</I>-nitrosylation, however the cellular substrates for NO-mediated <I>S</I>-nitrosylation are largely unknown. Here we describe the <I>S</I>-nitrosoproteome using a high-density protein microarray chip containing 16,368 unique human proteins. We identified 834 potentially <I>S</I>-nitrosylated human proteins. Using a unique and highly specific labeling and affinity capture of <I>S</I>-nitrosylated proteins, 138 cysteine residues on 131 peptides in 95 proteins were determined, defining critical sites of NO's actions. Of these cysteine residues 113 are novel sites of <I>S</I>-nitrosylation. A consensus sequence motif from these 834 proteins for <I>S</I>-nitrosylation was identified, suggesting that the residues flanking the <I>S</I>-nitrosylated cysteine are likely to be the critical determinant of whether the cysteine is <I>S</I>-nitrosylated. We identify eight ubiquitin E3 ligases, RNF10, RNF11, RNF41, RNF141, RNF181, RNF208, WWP2, and UBE3A, whose activities are modulated by <I>S</I>-nitrosylation, providing a unique regulatory mechanism of the ubiquitin proteasome system. These results define a new and extensive set of proteins that are susceptible to NO regulation via <I>S</I>-nitrosylation. Similar approaches could be used to identify other post-translational modification proteomes.</P>