Modified TOT, Canal TOT to mitigate mesh erosion in stress urinary incontinence surgery

( Jeong Jin Kim ),( Ji Ye Kim ),( Hwa Cheong Kim ),( Pomi Kim ),( Hae Sun Yang ),( Eun Ju Yun ),( Kyo Won Lee ) 대한산부인과학회 2016 대한산부인과학회 학술대회 Vol.102 No.-

목적: Teach a new procedure. 방법: Modified transobturator tape (TOT), Canal TOT procedure was developed and published in Journal of Urology on 2009 and it was effective for mitigating the complications of the conventional TOT procedure in short term followup. Between October 2006 and December 2011, 205 consecutive women with stress and mixed urinary incontinence underwent canal TOT procedure. Conventional TOT usually requires making a single vaginal medial incision. However Canal TOT requires making two vaginal later incisions. It makes possible to palpate arcus tendinus fascia and ischiopubic ramus. This finger guided method could reduce bladder and vascular injury by well positioned mesh anatomically. 결과: A minimum 5-year followup was available in patients. Mesh erosion was recorded in two patients (1.0 %) and mesh was surgically removed. The subjective and objective cure rate at 5 year were 77.8 % and 94.5% respectively. And other complications such as dyspareunia(2.8%),de novo urgency and urinary retention, prolonged urethral catheterization(2.1 %) show similar incidence at conventional TOT in previous studies. 결론: Canal TOT procedure is an effective minimal invasive procedure with satisfactory results for female SUI in long term. Compared to previous other studies about mesh erosion after TOT procedure (1.6-4.7 %), this technique might be useful in preventing mesh erosion, because the mesh was anatomically well positioned, and minimize the invisible paravaginal defect cystocele using a finger-guided method. And it could be safe for patients who are obese or have prior vaginal surgery.

A Variable Structure Approach to control the Active and Reactive Power for Doubly Fed Induction Generator

WON-SANG KIM,KYO-BEUM LEE,BYOUNG-CHANG JEONG,SEUNG-HO SONG 전력전자학회 2007 ICPE(ISPE)논문집 Vol.- No.-

The original direct power control (DPC) is known to give a fast response under transient conditions. However, active power, reactive power and current pulsations occur in steady-state operation. A family of variable structure controllers for the doubly fed induction generator (DFIG)-based wind turbine system is presented, using the principles of an active and reactive power controller known as modified DPC, and where variable structure control (VSC) and space-vector modulation (SVM) are combined to ensure high-performance operation. VSC scheme is designed following the modified DPC philosophy, which provides robust, fast, accurate active and reactive power controls without the problem of high chattering. Simulation results demonstrate that the proposed method preserves the effectiveness and robustness during variations of active and reactive power, rotor speed and converter DC-link voltage.

Uniform coating of molybdenum disulfide over porous carbon substrates and its electrochemical application

Jeong, Namjo,Kim, Han-ki,Kim, Won-sik,Choi, Ji Yeon,Han, Ji-hyung,Nam, Joo-youn,Hwang, Kyo Sik,Yang, SeungCheol,Jwa, Eun-Jin,Kim, Tae-Young,Park, Soon-Chul,Seo, Yong-Seog,Kim, Sung-in Elsevier 2019 Chemical engineering journal Vol.356 No.-

<P><B>Abstract</B></P> <P>The direct integration of two-dimensional nanostructures into macroscopic porous carbon substrates is essential for their practical use in potential applications, which is a big challenge due to the difficulty of securing uniform coating layers. In this work, we demonstrate a very simple and effective method for the direct coating of MoS<SUB>2</SUB> onto the surface of porous carbon structures having nano-sized pores, such as vertically aligned carbon nanotube arrays. A uniform coverage of MoS<SUB>2</SUB> over carbon substrates was achieved by chemical vapor deposition of gaseous species derived from starting precursors in a closed reactor. Transmission electron microscopy images, X-ray diffraction data, and Raman spectra confirmed the formation of highly crystalline MoS<SUB>2</SUB> layers. X-ray photoelectron and energy dispersive X-ray spectroscopies revealed highly uniform MoS<SUB>2</SUB> layers over the whole surface of the carbon substrates. Our approach is also applicable for the synthesis of MoS<SUB>2</SUB>/carbon fiber paper (CFP) hybrid structures. The electrochemical tests showed that the as-synthesized MoS<SUB>2</SUB>/CFP structures can serve as highly active cathodes for reverse electrodialysis.</P> <P><B>Highlights</B></P> <P> <UL> <LI> MoS<SUB>2</SUB>@VCNT and MoS<SUB>2</SUB>@CFP were prepared in this work. </LI> <LI> Uniform MoS<SUB>2</SUB> coating was achieved by CVD of gaseous species in the closed reactor. </LI> <LI> This approach is very powerful for MoS<SUB>2</SUB> coating over macroscopic porous substrates. </LI> <LI> MoS<SUB>2</SUB>/CFP can be a highly active cathode for reverse electrodialysis. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Sulforaphane blocks hypoxia-mediated resistance to TRAIL-induced tumor cell death.

Jeong, Jae-Kyo,Moon, Myung-Hee,Seo, Jae-Suk,Seol, Jae-Won,Lee, You-Jin,Park, Sang-Youel D. A. Spandidos 2011 MOLECULAR MEDICINE REPORTS Vol.4 No.2

<P>Hypoxia occurs frequently in various solid tumors and elicits a cellular response designed to improve cell survival through adaptive processes, thereby accelerating cancer progression and the development of chemotherapy resistance. Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), a member of the TNF superfamily, leads to tumor cell death via both intrinsic and extrinsic apoptotic signaling pathways. Hypoxia inhibits TRAIL-mediated apoptosis and attenuates the therapeutic activity of TRAIL in cancer management. Hypoxia-inducible factor-1관 (HIF-1관) plays a central role in tumor hypoxia by up-regulating gene expression related to angiogenesis, cancer invasion and anti-apoptosis. Sulforaphane (SFN), a phenethyl isothiocyanate, elicits HIF-1관 inactivation under hypoxia. This study investigated whether hypoxic inhibition of TRAIL-mediated tumor cell death is increased by SFN-mediated HIF-1관 instability. SFN induced cell death in various tumor cells, including SK-N-SH, SNU-638, HeLa and A549 cells, and showed cell cytotoxicity in hypoxia-exposed tumor cells. Western blot analysis showed that SFN treatment increased p53 and activated caspase-3 proteins, and decreased HIF-1관 activation under hypoxia. Under low-oxygen conditions, TRAIL-treated cells displayed inhibited apoptosis, while SFN-pre-treated cells exhibited stronger sensitization to TRAIL under the hypoxic conditions. SFN treatment enhanced TRAIL-induced activation of proteins, including caspase-3 and p53. SFN dose-dependently decreased HIF-1관 protein levels in cancer cells, which was mediated by decreased protein stability. This study demonstrated that SFN recovered hypoxia-mediated resistance to TRAIL via instability of HIF-1관, and also suggests that combination therapy with SFN and TRAIL may provide a novel strategy for treating hypoxic solid tumors.</P>

Enhancing the electroluminescence of OLEDs by using ZnO nanoparticle electron transport layers that exhibit the Auger electron effect

Jeong, Ki Won,Kim, Hyun Soo,Yi, Geum Ran,Kim, Chang Kyo Informa UK (TaylorFrancis) 2018 Molecular Crystals and Liquid Crystals Vol.663 No.1

Translocation of cellular prion protein to non-lipid rafts protects human prion-mediated neuronal damage.

Jeong, Jae-Kyo,Moon, Myung-Hee,Lee, You-Jin,Seol, Jae-Won,Park, Sang-Youel D.A. Spandidos 2012 International journal of molecular medicine Vol.29 No.3

<P>Prions are the causative agents of transmissible spongiform encephalopathies, such as variant Creutzfeldt-Jakob disease in humans. Cellular prion proteins (PrPC) connect with cholesterol- and glycosphingolipid-rich lipid rafts through association of their glycosyl-phosphatidylinositol (GPI) anchor with saturated raft lipids and interaction of their N-terminal regions. Our previous study showed that cellular cholesterol enrichment prevented PrP(106-126)-induced neuronal death. We have now studied the influence of membrane cholesterol in PrP(106-126)-mediated neurotoxicity and identified membrane domains involved in this activity. We found that PrPC is normally distributed in lipid rafts, but high membrane cholesterol levels as a result of cholesterol treatment led to the translocation of PrPC from lipid rafts to non-lipid rafts. Moreover, cholesterol-mediated PrPC translocation protects PrP(106-126)-mediated apoptosis and p-38 activation and caspase-3 activation. In a mitochondrial functional assay including mitochondrial transmembrane potential, cholesterol treatment prevented the loss of mitochondrial potential, translocation of Bax and cytochrome c by prion protein fragment. Our results indicate that modulation of the PrPC location appears to protect against neuronal cell death caused by prion peptides. The results of this study suggest that regulation of membrane cholesterol affects the translocation of PrPC, which in turn regulates PrP(106-126)-induced mitochondrial dysfunction and neurotoxicity.</P>

Comparison of effective dose for imaging of mandible between multi-detector CT and cone-beam CT

Jeong, Dae-Kyo,Lee, Sang-Chul,Huh, Kyung-Hoe,Yi, Won-Jin,Heo, Min-Suk,Lee, Sam-Sun,Choi, Soon-Chul Korean Academy of Oral and Maxillofacial Radiology 2012 Imaging Science in Dentistry Vol.42 No.2

Purpose : The aim of this study was to compare the effective dose for imaging of mandible between multi-detector computed tomography (MDCT) and cone-beam computed tomography (CBCT). An MDCT with low dose technique was also compared with them. Materials and Methods : Thermoluminescent dosimeter (TLD) chips were placed at 25 organ sites of an anthropomorphic phantom. The mandible of the phantom was exposed using 2 different types of MDCT units (Somatom Sensation 10 for standard-dose MDCT, Somatom Emotion 6 for low-dose MDCT) and 3 different CBCT units (AZ3000CT, Implagraphy, and Kavo 3D eXaM). The radiation absorbed dose was measured and the effective dose was calculated according to the ICRP 2007 report. Results : The effective dose was the highest for Somatom Sensation 10 (425.84 ${\mu}Sv$), followed by AZ3000CT (332.4 ${\mu}Sv$), Somatom Emotion 6 (199.38 ${\mu}Sv$), and 3D eXaM (111.6 ${\mu}Sv$); it was the lowest for Implagraphy (83.09 ${\mu}Sv$). The CBCT showed significant variation in dose level with different device. Conclusion : The effective doses of MDCTs were not significantly different from those of CBCTs for imaging of mandible. The effective dose of MDCT could be markedly decreased by using the low-dose technique.

Investigation of atomic-layer-deposited Al-doped ZnO film for AZO/ZnO double-stacked active layer thin-film transistor application

Jeong, Jun-Kyo,Yun, Ho-Jin,Yang, Seung-Dong,Eom, Ki-Yun,Chea, Seong-Won,Park, Jeong-Hyun,Lee, Hi-Deok,Lee, Ga-Won Elsevier S.A. 2017 Thin Solid Films Vol.638 No.-

<P><B>Abstract</B></P> <P>In this study, Al-doped zinc oxide (AZO) thin films with different Al concentrations fabricated by atomic layer deposition are investigated to determine the Al doping effect for AZO/ZnO double-stacked active layer thin-film transistor (TFT) applications. The AZO films are analyzed by X-ray diffraction, photoluminescence, and X-ray photoelectron spectroscopy, which show that the Al dopants affect the crystallinity, including the crystal direction and grain size, and reduce the deep trap sites such as oxygen vacancies (V<SUB>O</SUB>). The optimized Al doping concentration is about 2%. TFTs with an AZO (2%)/ZnO double-stacked active layer are fabricated and shown to exhibit a lower threshold voltage (V<SUB>th</SUB>), subthreshold slope, and V<SUB>th</SUB> shift under a positive gate-bias stress compared to ZnO single-layer devices. In the case of the on-current, however, the AZO stacked devices exhibit a smaller value. These electrical characteristics can be explained by V<SUB>O</SUB> suppression and altered crystal properties due to Al doping. For the field-effect mobility, the temperature dependence also reveals that the main transport mechanisms are thermionic and thermal field emission over the grain boundary in the AZO stacked devices. These results indicate that the AZO film properties depend strongly on the Al concentration and hence the ZnO-based devices can be optimized for specific application by Al doping.</P> <P><B>Highlights</B></P> <P> <UL> <LI> AZO thin films with different Al doping concentration were analyzed. </LI> <LI> Through physical analysis, 2% AZO showed the best characteristics. </LI> <LI> The use of AZO showed improved stability through gate bias stress measurement. </LI> <LI> The main transport mechanism was analyzed through temperature dependence of mobility. </LI> <LI> The main transport mechanism is thermionic and thermal field emission. </LI> </UL> </P>

High refractive index organic/inorganic hybrid films prepared by carbazole phenoxy based copolymers and titanium alkoxide

Jeong, Min Kyo,Choi, Won Tae,Ahn, Byung-Hyun,Gal, Yeong-Soon,Lim, Kwon Taek Taylor & Francis Inc. 2019 Molecular Crystals and Liquid Crystals Vol.686 No.1

Effectiveness of Rotor Off Fraction in Allogeneic Murine Bone Marrow Transplantation with Complete Disparity of Major Histocompatibility

Jeong, Dae Chul,Han, Chi Wha,Jin, Jong Youl,Kim, Dae Sik,Choi, Il Bong,Lee, Kyo Young,Kim, Won Il,Kim, Hack Ki,Kim, Chun Choo,Lee, Byung Churl,Imamura, Masahiro,Noga, Stephen J 대한조혈모세포이식학회 1998 대한조혈모세포이식학회지 Vol.3 No.1

연구배경 : 역류 원심성 세포분리는 세포의 서로 다른 침강속도의 특성을 이용하여 세포의 기능과 소실 없이 어느 특정 세포를 분리하는 방법으로서 골수로부터 T세포를 분리하는 매우 효과적인 물리적 세포분리 방법이다. 저자들은 주조직적합항원이 불일치 하는 쥐의 동종골수이식에서 역류 원심성 세포분리의 마지막 산물인 Rotor Off(R/O)분획의 효용성을 알아보고자 하였다. 방법 : 치사량의 방사선을 조사한 BLAB/C(H-2K^(d)) 쥐에게 C3H/HE(H-2K^(k)) 쥐의 골수를 이식하였다. 저자들은 역류 원심성 세포분리로서 T세포가 제거되고 조혈모세포가 농축된 R/O(rotor off)분획을 얻었고 실험군에 따라 이식하려는 골수를 조성하였다. 실험군을 3군으로 나누어 A군은 조작하지 않은 골수, B군은 R/O분획에 림프구가 농축된 분획으로부터 T세포를 추가한 골수, 그리고 C군은 R/O분획으로 구성된 골수 이었다. 조혈기능을 알아보기 위해 단핵구를 반고형배지에 14일간 배양하여 과립구/단핵구 코로니 빈도를 조사하였으며, 또한 이식된 골수의 특성을 확인하기 위해 flow cytometry를 이용하여 실험군에 따른 T세포와 조혈모세포의 분포를 조사하였다 이식후 매일 임상적인 이식편대 숙주병(graft versus host disease, GVHD)의 정도를 파악하여 생존유무를 확인하였다. 결과 :역류 원심성 세포분리기로 부하된 세포에 대한 전체 세포의 회복률은 약 71.45%이었으며 R/O분획으로 41.8%의 세포가 회복되었다. 역류 원심성 세포분리후의 각 분획의 10^(5) 의 단핵구에 대한 과립구/단핵구 코로니 빈도는 조작하지 않은 골수에서 21.2±1.33개, 17분획에서 23.68±2.23개, R/O분획에서 331.28±34.44개이었으며(Kruskal-Wallis test : x²=0.0044), 25와 28분획에서는 코로니의 형성을 확인할 수 없었다. 실험군에 따라 T세포와 조혈모세포의 분포는 조혈모세포가 조작되지 않은 골수(A군)에 비해 R/O분획(C군)에서는 1.96배(p<0.05), B군에서는 1.8배(p<0.05) 농축되었고 조작된 골수 사이와 조혈모세포의 농축정도 사이에는 유의한 차이가 없었다. 조혈모세포는 부하된 전체 양중 80.0±10.1%가 R/O분획으로 회복되었다. T세포가 R/O분획(C군)에서는 88.1% 감소되었으나(p<0.05), B군에서는 15.7%로 감소되어 A군의 T세포분포와 차이가 없었다. 이식후 90일째 각군의 생존률은 A군이 0, B군이 0.2, 그리고 C군이 0.8로 현저한 차이가 있음을 확인하였다.(p=0.0006). 실험군 사이에 GVHD의 심각도(p=0.0043)와 축적된 점수의 증가추세(p=0.02)는 매우 유의하게 달랐다. 결론 : 역류 원심성 세포분리로 얻은 R/O분획은 다량의 T세포가 제거되고 조혈모세포가 농축되었다. R/O분획을 이용한 주조직적합항원이 불일치하는 쥐의 골수이식에서 장기간의 이식편의 생착과 미미한 GVHD로 생존율이 증가되었다. 앞으로 임상에서 주조직적합항원이 일치하는 비혈연간 또는 주조직 적합항원이 불일치하는 혈연간 동종골수이식에서 역류 원심성 세포분리가 유용하리라 생각된다. Counterflow centrifugal elutriation (CCE) has been a highly efficient physical method for separating T cell from bone marrow(BM) without impairing cell function and yield. To investigate the usefulness of CCE for T cell depletion or hematopoietic stem cell consentration from bone marrow, the lymphocyte-depleted rotor off(R/O) fraction obtained during the final steps of CCE from C3H/He(H-2K^(k)) was transplanted into lethally irradiated (875 cGy) BALB/C(H-2k^(d)) mice. After CCE, the total cell recovery was about 71.4% with high viability. Morphologically, the R/O fraction contained abundant mononulcear cells with a few lymphocytes. The number of colony forming units for granulocyte/monocyte (CFU-GM) per 10^(5) mononuclear cells was significantly higher in the R/O faction (331.28±34.44) than UN (21.12±1.33) or fractions collected at flow rates (FR) of 17ml/min(FR 17) (23.68±2.23) (x²=0.0044). Neither FR 25 nor FR 28 contained CFU-GM colonies. The concentration of Sca-1+ stem cells in the R/O fraction was significantly higher (1.96 fold) than in UN, and 88.1% of Thy-1.2^(+) T cells were eliminated in the R/o fraction (p<0.05). The stem cell (Sca-1^(+)) recovery in the R/O fraction was 80.0±10.1%. Mice receiving UN marrow suffered from severs GVHD and all died within 7 days after BMT (Group A). Of interest, mice receiving the R/O fraction to which T cells were added to adjust their number to equal the number of T cells found in UN marrow (Group B) developed severe GVHD and only one of five survived (probability of survival; 20%). Mice receiving the R/O fraction (Group C) had no GVHD and four of five (probability of survival ; 80%) survived for at least 90 days. The probability of survival (p=0.0006), the severity of GVHD (p=0.0043) and the progression speed of GVHD (p=0.02) were the lowest in group C. In conclusion, we suggest that transplantation with elutriated R/O cells have the advantages of stable engraftment and tolerable GVHD in murine BMT with complete MHC disparity. This could be directly applicable to patients at high risk for GVHD in upcoming clinical trials.