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      • First ultrastructural data on the human tapeworm Taenia asiatica eggs by scanning and transmission electron microscopy (SEM, TEM)

        Galá,n-Puchades, M. Teresa,Yang, Yichao,Marcilla, Antonio,Choe, Seongjun,Park, Hansol,Osuna, Antonio,Eom, Keeseon S. Springer-Verlag 2016 Parasitology research Vol.115 No.9

        <P>Humans are definitive hosts of three species of the Taenia genus, namely Taenia solium, Taenia saginata and Taenia asiatica. The relative novelty of the latter explains the lack of knowledge concerning certain relevant aspects related to this parasite, such as its definite geographical distribution and whether its eggs can infect humans or not. So far, only the eggs of T. solium are known to be infective for humans, producing cysticercosis. Although eggs contain the infective stage, the oncosphere, there is a lack of research on the ultrastructure of eggs of human taeniids. We show, for the first time, the ultrastructure of eggs of T. asiatica by means of SEM and TEM analyses. We detected all the envelopes, namely the egg shell, vitelline layer, outer embryophoric membrane, embryophore, granular layer, basal membrane, oncospheral membrane and oncospheral tegument. Hooks surrounded by myofibrils and glycogen-like particles, the two types of secretory granules of the penetration glands, as well as several nuclei and mitochondria were also revealed in the oncospheres. In addition to the already known structures in eggs from other Taenia species, the presence of two types of small vesicles is described herein, possibly corresponding to exosomes and ectosomes because of their shape and size, which could participate in the host/parasite intercellular communication.</P>

      • KCI등재

        From Media to Transmedia: Transforming Teaching and Learning Strategies in a Digital Culture

        Lorenzo Galés, Neus 영상영어교육학회 2019 영상영어교육 (STEM journal) Vol.20 No.4

        During the last century, education through media has evolved and generalized both in formal and informal learning contexts. Using films in the classroom helped minimize the differences between academic studies, guided education, and self-access (learner’s autonomous access to content). Today, teaching through media implies using new technologies such as mobile apps, video games, autonomous learning platforms or social media in the classroom. Educators will find new challenges and opportunities if they manage this transmedia universe in their professional development. Transmedia activities are connected to developing multi-literacies in a collective digital culture, active citizenship in a sustainable world, and ethical awareness for community building. Traditional individual language-learning skills (speaking, reading, listening, writing) are usually seen as the basics of communication, but academic research shows that transliteracy, inter-comprehension and plurilingual skills are needed for cultural awareness, social interaction and global communicative competence (including abilities such as debating, mediating, coaching, coding and decoding). Evolving from educational media to transmedia requires changing teaching attitudes and adopting specific approaches, techniques and tools to generate much more interactive activity, integrated tasks and integrative projects. In this communicational ecosystem, researchers explore new tools and strategies to illustrate learning processes, teachers discover different approaches methodologies, and students learn to participate in their own learning process.

      • KCI등재

        Bioactive Properties of Tynanthus panurensis (Bureau) Sanwith Bark Extract, the Amazonian “Clavo Huasca”

        Lidia Morales,Nuria Acero,Antonio Galán,Carmen Perez-García,Luis Fernando Alguacil,Dolores Muñoz-Mingarro 한국식품영양과학회 2011 Journal of medicinal food Vol.14 No.9

        Tynanthus panurensis (Bureau) Sanwith (Bignoniaceae) is a liana vine used in traditional Amazonian medicine as a tonic and energizer as well as a treatment for rheumatism. These traditional indications prompted this study of the antioxidant and anti-inflammatory activities of T. panurensis bark extract (ETP). Phytochemical analysis of ETP showed the presence of saponins and a high concentration of phenols and flavonoids. A battery of in vitro tests revealed that the extract has free radical–scavenging antioxidant properties and reduces microsomal lipid peroxidation, uric acid synthesis, and tumor necrosis factor-α production. The anti-inflammatory properties of ETP were further confirmed in vivo in a rat carrageenan edema model, in which the extract exhibited a potent activity. These results support the idea that T. panurensis bark extract could be beneficial for treating inflammation and are in agreement with one of the main traditional uses of this plant.

      • KCI등재

        Sex differences in QEEG in adolescents with conduct disorder and psychopathic traits

        Ana Calzada-Reyes,Alfredo Alvarez-Amador,Lidice Galán-García,Mitchell Valdés-Sosa 대한임상신경생리학회 2019 Annals of Clinical Neurophysiology Vol.21 No.1

        Background: Sex influences is important to understand behavioral manifestations in a large number of neuropsychiatric disorders. We found electrophysiological differences specifically related to the influence of sex on psychopathic traits. Methods: The resting electroencephalography (EEG) activity and low-resolution brain electromagnetic tomography (LORETA) for the EEG spectral bands were evaluated in 38 teenagers with conduct disorder (CD). The 25 male and 13 female subjects had psychopathic traits as diagnosed using the Antisocial Process Screening Device. All of the included adolescents were assessed using the Diagnostic and Statistical Manual of Mental Disorders, Fourth Edition, Text Revision (DSM-IV-TR) criteria. The visually inspected EEG characteristics and the use of frequency- domain quantitative analysis techniques are described. Results: Quantitative EEG (QEEG) analysis showed that the slow-wave activities in the right frontal and left central regions were higher and the alpha-band powers in the left central and bitemporal regions were lower in the male than the female psychopathic traits group. The current source density showed increases in paralimbic areas at 2.73 Hz and decreases in the frontoparietal area at 9.37 Hz in male psychopathics relative to female psychopathics. Conclusions: These findings indicate that QEEG analysis and techniques of source localization can reveal sex differences in brain electrical activity between teenagers with CD and psychopathic traits that are not obvious in visual inspections.

      • KCI등재

        A Finite Element-Based Methodology for the Thermo-mechanical Analysis of Early Age Behavior in Concrete Structures

        H. Cifuentes,F. Montero-Chacón,J. Galán,J. Cabezas,A. Martínez-De la Concha 한국콘크리트학회 2019 International Journal of Concrete Structures and M Vol.13 No.6

        This paper presents a general procedure based on fracture mechanics models in order to analyze the level of crack-ing and structural safety in reinforced concrete elements at early ages, depending on the stripping time. Our proce-dure involves the development of a thermo-mechanical numerical model based on the finite element method that accounts for the change in the mechanical properties of concrete with time. Moreover, fracture mechanisms are analyzed by means of a material damage model, which is characterized via specific experimental results obtained for standard specimens and notched beams under three-point bending testing. The loading conditions are both thermal and mechanical, and are obtained from the hydration process for a given concrete dosage. The presented methodol-ogy allows for the determination of the optimal stripping time, whereas it helps assessing the analysis of the cracking and the stress states of the elements under consideration. A practical application, namely the analysis of a retaining wall, is used to validate our methodology, showing its suitability in engineering practice.

      • KCI등재

        새우젓으로부터 혈전과 chitin 분해능을 지닌 균주 Bacillus licheniformis SC082의 분리 및 특성

        조은경(Eun Kyung Cho),정유정(Yu Jung Jung),갈상완(Sang-Wan Gal),최영주(Young Ju Choi) 한국생명과학회 2009 생명과학회지 Vol.19 No.10

        전통발효식품인 새우젓으로부터 혈전과 chitin 분해력이 우수한 균을 분리하였으며 16S rRNA 염기서열 분석으로 B. licheniformis와 가장 유사한 균주임을 확인하였다. 이 균주를 B. licheniformis SC082로 명명하였고, 최적 생육조건은 37℃, pH 7.0, 염 농도 6%로 확인되었다. 이 균주의 기질특이성을 조사한 결과, 우수한 혈전분해력을 나타냈으며 1% 농도의 colloidal chitin의 첨가에 의하여 chitinase 활성이 증가됨을 관찰할 수 있었다. 또한 지질 분해능은 없었고 약한 skim milk 분해력을 가지고 있었다. SDS-PAGE와 zymogram 분석 결과, 이 균은 혈전분해효소 isozyme과 chitinase isozyme을 생성하는 균주로 확인되었다. 그 대략적인 분자량은 각각 22.0, 66.0, 72.0 kDa과 55.0, 62.0 kDa이었다. B. licheniformis SC082 균주가 생성하는 혈전분해효소는 pH 9.0 그리고 50℃까지 안정하게 유지되었고, 이와 더불어, chitinase 활성은 pH 5, 45℃일 때 높게 나타났다. B. licheniformis SC082 균주의 DPPH 전자공여능법에 의한 항산화력은 농도의 증가에 따라 상승되었는데 20 ㎍의 균상등액에 대한 항산화력은 약 31%으로 나타났다. Shrimp Jeot-Gal is a popular traditional Korean fermented seafood and has been used for seasoning. We isolated a bacterium showing strong extra-cellular fibrinolysis and chitinase activity from shrimp Jeot-Gal and the strain was designated SC082. SC082 was identified as Bacillus licheniformis by 16S rRNA sequence homology search. B. licheniformis SC082 exhibited optimum temperature, pH, and salt concentration at 37℃, pH 7.0, and 6%, respectively. Substrate specificity of the culture supernatant from B. licheniformis SC082 was detected in fibrin, skim milk, and chitin plate. The fibrinolytic activity was highly maintained up to 50℃ at a pH of 7.0 for 3 hr and was stable up to pH 9.0 at 37℃ for 3 hr. The chitinase activity was remarkably induced by addition of 1.0% colloidal chitin and the pH and temperature optima of the enzyme were 5.0 and 45℃, respectively. In sodium dodecyl sulfate-polyacrylamide gel electrophoresis and zymogram analysis, this strain produced three fibrinolytic isozymes and two chitinase isozymes. The approximate molecular weights of the putative fibrinolytic enzymes were 23.0, 62.0, and 72.0 kDa and those of the chitinases were 62.0 and 55.0 kDa, respectively. The antioxidant activity of SC082 was also measured by using 2,2-diphenyl-l-picryl-hydrazyl (DPPH) free radical. The DPPH radical scavenging was slightly increased in a dose-dependent manner.

      • KCI우수등재

        경찰관의 직무몰입이 직무스트레스, 직무만족 및 조직몰입에 미치는 영향

        제갈욱 ( Gal Uk Jae ),김병규 ( Byung Kyu Kim ),제갈돈 ( Gal Don Jae ) 한국행정학회 2015 韓國行政學報 Vol.49 No.4

        많은 조직행태론적 연구에 의하면 조직구성원들이 자신들의 직무에 대하여 심리적 확신을 가지지 못하고 있으면 조직의 기능적 운영에 손상을 가져 올 것이라고 기대할 수 있다. 아마 자신들의 직무에 보다 많이 몰입하는 조직구성원들은 그렇지 못한 사람들에 비하여 보다 낮은 수준의 직무스트레스를 느끼는 반면 보다 높은 수준의 직무만족과 조직몰입을 가질 것으로 예상할 수 있다. 일반 조직구성원 특히 일반 공무원들보다 경직된환경에서 근무하는 경찰관의 경우도 일반적인 가정에 부합하는지가 본 연구의 의문이다. 이를 위하여 본 연구는 부산 지역의 경찰을 중심으로 직무스트레스, 직무만족 및 조직몰입에 대한 직무몰입의 효과를 조사하였다. 경찰관에 대한 설문자료에 대한 다변량분석 결과에 의하면, 직무몰입은 연구의 가정과는 달리 직무스트레스에 대한 부정적인 영향을 발견할 수 없었다. 하지만 직무몰입의 직무만족과 조직몰입에 대한 긍정적인 영향은 확인되었다. 향후 경찰조직에 대한 연구 및 실제를 위한 연구결과의 정책적 함의도 제기되었다. Police officers who harbor negative attitudes toward their jobs can be detrimental to the functional operation of the organization. Presumably, personnel who are more involved with their work should have lower levels of stress and higher levels of job satisfaction and organizational commitment, compared with those who are not as involved. This study examined the effects of job involvement on job stress, job satisfaction, and organizational commitment at a large police system in Pusan. Based on multivariate analyses of police officer survey data, the results revealed that job involvement was negatively related to job stress and positively related to both job satisfaction and organizational commitment. The implications of these findings for police research and practices are also considered.

      • KCI등재

        지역사회경찰활동의 성공적인 집행을 위한 경찰의 역할

        제갈돈(Jae gal Don),제갈욱(Jae gal Uk),장석헌(Chang Suk Heon) 한국공안행정학회 2005 한국공안행정학회보 Vol.20 No.-

        급속한 사회변화는 경찰의 새로운 기능과 역할을 요구하게 되었고, 이러한 요구에 부응하기 위하여 경찰은 과거의 단순한 역할에서 탈피하여 경찰서비스를 질적으로 개선하려고 노력하여야 한다. 본 연구의 목적은 지역사회경찰활동의 정의를 명확히 하고, 성공적인 지역사회경찰활동과 연관된 경찰조직의 특징을 경험적으로 파악하며, 실무자들에게 어떤 변수들이 성공적인 지역사회경찰활동에 영향을 주는지를 이해하는 것이다. 우리나라 경찰에 대한 조사에서는 문제해결과정에서 일선경찰관의 재량권 허용이 13개 지역사회경찰활동의 성공적 집행과 관련된 변수들 중에서 가장 저조한 참여율을 나타내고 있다. 따라서 지역사회경찰활동의 집행을 외형적으로 보여줄 수 있는 프로그램의 적극적인 시행 이전에 지역사회경찰활동의 성공을 위한 근본적인 조직문화의 변화가 지역사회경찰활동의 성공적 집행을 보장해 줄 수 있을 것이다. 또한 지역사회경찰활동의 재교육 프로그램 개선을 위한 방안을 모색하여야 할 것이다. A rapidly changing society needs new functions and roles of police. Corresponding to this demands, police must change simple roles of police in the past and try to improve the quality of police service. The purpose of this study is to provide an understanding of the factors related to the successful implementation of community policing. More specifically, this study include clarifying the definition of community policing, empirically identifying the characteristics of police departments that are associated with successful community policing, and providing practitioners with an understanding of how characteristics influence successful community policing. Research findings say that, among 13 factors related to the success of community policing, policemen's discretion resolving community problems appears the major problem for the successful community policing. Prior to implementing community policing programs, for the successful implementation of community policing, it can be essential to change fundamental organization culture and to improve reeducation programs of community policing.

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