RNA design rules from a massive open laboratory

Lee, Jeehyung,Kladwang, Wipapat,Lee, Minjae,Cantu, Daniel,Azizyan, Martin,Kim, Hanjoo,Limpaecher, Alex,Yoon, Sungroh,Treuille, Adrien,Das, Rhiju National Academy of Sciences 2014 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF Vol.111 No.6

<P>Self-assembling RNA molecules present compelling substrates for the rational interrogation and control of living systems. However, imperfect in silico models—even at the secondary structure level—hinder the design of new RNAs that function properly when synthesized. Here, we present a unique and potentially general approach to such empirical problems: the Massive Open Laboratory. The EteRNA project connects 37,000 enthusiasts to RNA design puzzles through an online interface. Uniquely, EteRNA participants not only manipulate simulated molecules but also control a remote experimental pipeline for high-throughput RNA synthesis and structure mapping. We show herein that the EteRNA community leveraged dozens of cycles of continuous wet laboratory feedback to learn strategies for solving in vitro RNA design problems on which automated methods fail. The top strategies—including several previously unrecognized negative design rules—were distilled by machine learning into an algorithm, EteRNABot. Over a rigorous 1-y testing phase, both the EteRNA community and EteRNABot significantly outperformed prior algorithms in a dozen RNA secondary structure design tests, including the creation of dendrimer-like structures and scaffolds for small molecule sensors. These results show that an online community can carry out large-scale experiments, hypothesis generation, and algorithm design to create practical advances in empirical science.</P>

Experimental Study of brake pipe oil leak by sealing performance impro vement

Jeehyung Lee(이지형),Ilmin Hong(홍일민),Sunho Kim(김선호) 한국자동차공학회 2013 한국자동차공학회 부문종합 학술대회 Vol.2013 No.5

The study presents how the characteristic of brake tube nut surface treatment can be affected brake oil leak at pipe connection area. Brake oil leak issue is one of the most chronic problems in Automobile manufacturing and as the issue is normally approached by torque increase for containment or improving part quality and assembly quality, the problem is fluctuated due to the quality control. According to vehicle corrosion requirement is gradually severe and workers require easier finger-start for brake pipe installation, brake tube nut surface treatment is selecting ZnNi rather than Zn. If we control the same coefficient of friction spec for both, there is no difference for sealing performance in theoretical. Even though Zn surface treatment is regarded better sealing performance than ZnNi empirically by sealing mark test result, there was no objective evidence of relationship between sealing mark and brake oil leak and it is very difficult to define with CAE analysis So, we approached this issue as below - Comparison brake pipe flare and tube nut - Comparison leak test with brake pipe and tube nut each supplier - Comparison leak test with different machining method to increase thread length - Comparison leak test with pipe misalignment condition - Comparison seal mark test according to tube nut friction of surface treatment - Comparison seal mark test for tube nut surface treatment (ZnNi vs Zn) From the experimental study, we verified what dominant factor is for brake pipe sealing. We checked the ZnNi surface treatment has lighter seal mark than Zn cause by increasing friction and found the friction increase because ZnNi layer of tube nut at thread area can easily be damaged at aluminum port without anodized surface. As a result of the experimental study, tube nut surface treatment shouldn’t be selected ZnNi because most of modulator material are aluminum without anodized surface. If ZnNi should be applied for corrosion improvement and easier finger-start, it should be considered the torque increasing.

요양소의 담을 넘어

이지형(Jeehyung Lee) 동아시아일본학회 2018 일본문화연구 Vol.0 No.68

본 논문은 1950년대 일본 한센병문학 위기 논쟁의 실체와 진행과정을 한센병문학이 태동된 1930년대부터 50년대에 이르기까지의 한센병자 당사자 담론을 중심으로 고찰하였다. 특히 한센병문학의 정의, 한센병문학의 소멸 위기 및 사회화 문제에 대해 어떻게 진단, 모색하는지에 초점을 맞춰 살펴보았다. 논쟁의 계보를 살펴보면, 1930년대는 『생명의 초야』의 작가 호조 다미오의 등장과 더불어 한센병문학의 정의 및 의의에 대한 근본적 논의가 촉발되었다. 나문학은 결코 특수한 문학이 아니라는 주장에도 불구하고 나병 없이는 잉태될 수 없었던 한센병문학의 딜레마가 여실히 확인된다. 1953년 나병예방법 개정을 둘러싼 공방 속에 논쟁은 더욱 심화된다. 그 종착점이 1958년의 특집기획 좌담회이다. 좌담회 내용이 실린 두 특집기사, 「문학에의 지향」과 「「문학에의 지향」을 읽고」에서 주목되는 것은 고이즈미 다카유키와 나가카미 게스케이다. 요양소 내부에 여전히 무게중심을 두는 나가카미의 입장에서 ‘세계의 일원이라는 자각’에 기초해 요양소의 담을 넘어 바깥세계를 지향해야 한다는 고이즈미의 주장으로 이동해 가는 과정이 곧 논쟁 전체를 상징적으로 대표하고 있기 때문이다. This paper examines the reality of the 1950`s Japanese Hansen`s disease literacy crisis and its progress. The main consideration is the discourse of the Hansen`s disease patient parties from the 1930"s until the 1950"s when the Hansen`s literature began. In particular, I focused on the definition of Hansen`s disease literature, how to diagnose and search for the crisis of extinction and socialization of Hansen`s disease literature. Looking at the genealogy of controversy, in the 1930s, with the emergence of Tamio Hojo, writer of “The Beginning Night of Life,” a fundamental debate about the definition and significance of Hansen`s disease literacy was sparked. Despite the claim that Hansen`s literature is not a special literature, the dilemma of the Hansen`s disease literacy that could not have been conceived without Hansen`s disease is confirmed. The controversy deepens in the struggle over the revision of the ‘leprosy prevention law’ in 1953. Its end point is the special planning symposium of 1958. It is Takayuki Koizumi and Nagakami Gesuke who are attracting attention in two feature articles on which the content of the symposium was posted, “the direction to literature” and “reading 「the direction to literature」”. Because the process of moving to the claim of Koizumi that Hansen’s disease literature should go beyond the walls of the sanatorium to the outside world based on the “awareness of being a member of the world” from Nagakami"s position still centered on the inside of the sanatorium represents the structure of entire controversy.

보령호의 수질과 수자원 보호를 위한 유역모델(HSPF)과 호수모델(EFDC)의 통합 적용 방안

이어진 ( Lee Eojin ),이승문 ( Lee Seungmoon ),이지형 ( Lee Jeehyung ),김재영 ( Kim Jaeyoung ),서동일 ( Seo Dongil ) 한국물환경학회 2024 한국물환경학회·대한상하수도학회 공동 춘계학술발표회 Vol.2024 No.-

Lateral Control for Autonomous Lane Keeping System on Highways

Chang Mook Kang,Jeehyung Lee,Sung Gu Yi,Soo Jung Jeon,Young Seop Son,Wonhee Kim,Seung-Hi Lee,Chung Choo Chung 제어로봇시스템학회 2015 제어로봇시스템학회 국제학술대회 논문집 Vol.2015 No.10

In this paper, we propose a lateral control scheme for autonomous lane keeping system on highways. Three main techniques are proposed, to improve the lane-keeping performance and and to reduce the ripple in the yaw rate on highways. First, we propose a virtual lane prediction method to cope with the momentary failure of lane detection. Second, we innovate an approach to steering wheel angle control based on torque overlay for the EPS of the LKS. Finally, the multi-rate lane-keeping control scheme is proposed to improve the lane-keeping performance and to reduce the ripple in the yaw rate. The performance of the proposed method was evaluated via experiments.

한국인에서 시행한 가동성 슬관절 단일 구획 치환술의 합병증

김경태(Kyung Tae Kim),이송(Song Lee),김지형(Jeehyung Kim),강민수(Min Su Kang),구기혁(Ki Hyuk Koo) 대한정형외과학회 2017 대한정형외과학회지 Vol.52 No.4

목적: 한국인에서 시행한 가동형 슬관절 단일 구획 치환술 후 발생한 발생한 합병증의 원인, 종류 및 양상 등을 분석하고 이를 외국에서 발표된 기존 논문들과 비교하여 한국인에서 시행한 가동형 슬관절 단일 구획 치환술의 적합성 여부를 알아보고자하 였다. 대상 및 방법: 2002년 1월부터 2015년 12월까지 본원 정형외과에서 가동형 치환물을 사용하여 내측 슬관절 단일 구획 치환술을 시행하고 1년 이상 추시가 가능했던 1,325명(1,560예)을 대상으로 하였다. 정기적 추시를 통해 임상적 평가 및 방사선적 평가를 시행하고자 하였고 추시 중 합병증이 발생한 예들을 후향적으로 분석하여 합병증의 원인을 조사하고 확인하였으며 수술 후 합병증이 발생한 기간을 측정하고 시행한 치료 방법을 분석하였다. 결과: 총 1,560예의 가동형 슬관절 단일 구획 치환술 후 101예(6.5%)에서 합병증이 발생하였다. 가동성 삽입물의 단독 탈구가 52예(3.3%)로 가장 많았으며 전체 합병증 중 절반 이상(51.5%)을 차지하였다. 그 외 합병증으로 치환물의 해리 16예(1.0%), 반대측 구획으로의 관절염의 진행 9예(0.6%), 폴리에틸렌 삽입물의 마모 및 파손 5예(0.3%), 치환물 주위 골절 4예(0.3%), 치환물의 충돌 3예(0.2%), 내측 인대 손상 2예(0.1%), 관절 강직 1예(0.1%) 및 원인을 알 수 없는 통증으로 인한 실패가 1예(0.1%) 등이 있었으며 감염으로 인한 실패는 8예(0.5%)였다. 가동형 슬관절 단일 구획 치환술 후 합병증이 발생할 때까지의 기간은 평균 5.2년이었고 그 중 가동성 삽입물의 단독 탈구는 수술 후 평균 4.3년, 치환물의 해리는 수술 후 평균 6.5년, 반대측 구획으로의 관절염으로 인한 실패는 수술후 평균 11.2년에 발생하였다. 총 101예의 합병증 중 68예는 전치환술로, 1예는 단일 구획 치환술로 재치환술을 시행하여 치료하였으며, 23예는 단순 가동성 삽입물의 교환으로 치료하였다. 그 외 관절경술 3예, 내측 인대 봉합술 2예, 관혈적 정복술 및 내고정술 2예, 도수정복술 및 내고정술 1예, 도수 조작술 1예 등을 시행하였다 결론: 한국인에서 시행한 가동형 슬관절 단일 구획 치환술 후 발생한 합병증을 분석한 결과 가동성 삽입물의 탈구가 특히 많이 발생함을 알 수 있었으며 반대측 구획으로의 관절염의 진행 또는 폴리에틸렌 삽입물의 마모로 인한 실패는 상대적으로 적었다. 따라서 한국인에서 가동형 슬관절 단일 구획 치환술을 시행할 경우 가동성 삽입물의 탈구에 대한 충분한 대비와 함께 환자에 대한 사전 설명과 교육이 필요할 것으로 생각된다. Purpose: To evaluate the causes and modes of complications after unicompartmental knee arthroplasty (UKA) in Korean patients, and to identify the adaptability of mobile-bearing UKA for Korean patients by analyzing its complications. Materials and Methods: Between January 2002 and December 2015, a total of 1,325 patients (1,560 cases) who underwent mobile-bearing medial UKA and were followed-up for more than 1 year were included in this study. We analyzed the complications of UKA retrospectively, and investigated the mode of complications, mean time to reoperation, and method of treatment. Results: We observed a total of 101 complications (6.5%) after mobile-bearing UKA. The most prevalent complication was dislocation of mobile-bearing (n=52, 3.3%), accounting for more than half of the complications. Other complications include component loosening (n=16, 1.0%), progression of arthritis in lateral compartment (n=9, 0.6%), polyethylene wear and breakage (n=5, 0.3%), periprosthetic fracture (n=4, 0.3%), impingement (n=3, 0.2%), medial collateral ligament (MCL) injury (n=2, 0.1%), arthrofibrosis (n=1, 0.1%), unexplained pain (n=1, 0.1%) and infection (n=8, 0.5%). At a mean of 5.2 years post-UKA, complications occurred in our patients. The mean time interval from UKA to the development of mobile-bearing dislocation, component loosening, and progression of arthritis to the lateral compartment was a 4.3 years, 6.5 years, and 11.2 years, respectively. Complications were treated with a conversion to total knee arthroplasty in 68 cases, revision UKA in 1 case, and simple bearing change in 23 cases. Remaining complications were treated with arthroscopic management (n=3), MCL repair (n=2), open reduction and internal fixation (n=2), closed reduction and internal fixation (n=1), and manipulation (n=1). Conclusion: The incidence of mobile-bearing dislocation after mobile-bearing UKA was especially higher in Korean patients. However, the progression of arthritis in the lateral compartment and polyethylene wear were relatively lower compared with Western counterparts. Therefore, it is encouraged that Korean patients are provided with sufficient preparation for mobile-bearing dislocation and education prior to surgery when performing mobile-bearing UKA.