여대생에서 혈청 pro-hepcidin 농도와 철분 영양 상태와의 관계

정자용(Chung Jayong) 韓國營養學會 2005 Journal of Nutrition and Health Vol.38 No.9

Protective effects of baicalein treatment against the development of nonalcoholic steatohepatitis in mice induced by a methionine cholinedeficient diet

Jiwon Choi,Jayong Chung 한국영양학회 2023 Journal of Nutrition and Health Vol.56 No.6

Purpose: Baicalein, a natural flavone found in herbs, exhibits diverse biological activities. Nonalcoholic steatohepatitis (NASH) is an irreversible condition often associated with a poor prognosis. This study aimed to evaluate the effects of baicalein on the development of NASH in mice. Methods: Male C57BL/6J mice were randomly divided into four groups. Three groups were fed a methionine-choline-deficient (MCD) diet to induce NASH and were simultaneously treated with baicalein (at doses of 50 and 100 mg/kg) or vehicle only (sodium carboxymethylcellulose) through oral gavage for 4 weeks. The control group was fed a methionine-choline-sufficient (MCS) diet without the administration of baicalein. Results: The baicalein treatment significantly reduced serum levels of alanine aminotransferase and aspartate aminotransferase, suggestive of reduced liver damage. Histological analysis revealed a marked decrease in nonalcoholic fatty liver activity scores induced by the MCD diet in the mice. Similarly, baicalein treatment at both doses significantly attenuated the degree of hepatic fibrosis, as examined by Sirius red staining, and hepatocellular death, as examined by the terminal deoxynucleotidyl transferase dUTP nick end labeling assay. Baicalein treatment attenuated MCD-diet-induced lipid peroxidation, as evidenced by lower levels of hepatic malondialdehyde and 4-hydroxynonenal, demonstrating a reduction in oxidative stress resulting from lipid peroxidation. Moreover, baicalein treatment suppressed hepatic protein levels of 12-lipoxygenase (12-Lox) induced by the MCD diet. In contrast, baicalein enhanced the activities of antioxidant enzymes such as superoxide dismutase, catalase, and glutathione peroxidase. Additionally, baicalein treatment significantly reduced hepatic non-heme iron concentrations and hepatic ferritin protein levels in mice fed an MCD diet. Conclusion: To summarize, baicalein treatment suppresses hepatic lipid peroxidation, 12-Lox expression, and iron accumulation, all of which are associated with the attenuation of NASH progression.

The effects of time-restricted feeding (TRF) on glucose tolerance and skeletal muscle mass and function in sarcopenic obese mice

Sujin Gwon,Jayong Chung 한국식품영양과학회 2022 한국식품영양과학회 학술대회발표집 Vol.2022 No.10

Effects of Chronic Lead Exposure on the Expression of Brain Iron Transporters and Behavioral Development in Young Rats

Sugyoung Oh,Jayong Chung 한국식품영양과학회 2014 한국식품영양과학회 학술대회발표집 Vol.2014 No.10

Effects of Obesity on Zinc Nutritional Status and Zinc Transporter Expression in Mice Fed a High-Fat Diet

Byulchorong Min,Jayong Chung 동아시아식생활학회 2015 동아시아식생활학회 학술발표대회논문집 Vol.2015 No.10

고지방식이 유도 비만 마우스에서 아연 보충이 골격 근육의 아연 수송체 ZIP7 수준과 혈당 조절에 미치는 영향

주치앤징(Qianjing Zhu),정자용(Jayong Chung) 한국영양학회 2021 Journal of Nutrition and Health Vol.54 No.6

본 연구는 고지방식이로 유도한 비만 동물모델에서 아연의 식이를 통한 보충 급여가 혈당 조절과 골격 근육의 ZIP7의 작용에 미치는 영향에 대해 살펴보았다. 고지방식이를 공급한 HF군은 정상 대조군에 비하여 단위 체중당 골격 근육 무게가 유의하게 감소하였으며, 혈당은 유의적으로 증가하였다. 고지방식이와 함께 아연을 보충 공급한 HF+Zn군은 아연을 보충하지 않은 HF군과 비교하여, 공복 혈당과 경구 포도당 부하 후 혈당 증가 면적이 유의하게 감소하였다. 또한, HF+Zn군은 HF군에 비해 골격 근육의 ZIP7 단백질 수준이 유의하게 증가하였으며, AKT 활성과 GLUT4 단백질 수준도 유의하게 증가하는 것으로 나타났다. 이상의 결과를 종합해 볼 때, 아연 보충은 비만으로 인한 고혈당 증세를 완화하는 효과를 나타내며, 이는 골격 근육에서의 ZIP7 아연 수송체에 의한 당 대사 조절과 관련이 있을 것으로 생각된다. Purpose: The zinc transporter ZIP7 is known to regulate glucose metabolism in skeletal muscles, and skeletal muscles are known to play a critical role in glycemic control. The present study examines the effects of dietary zinc supplementation on the blood glucose concentration and expression of ZIP7 in skeletal muscle obtained from obese mice fed a high-fat diet (HF). Methods: C57BL/6J male mice were divided into three groups and were administered either a HF (60% of total calories from fat), HF supplemented with zinc (HF+Zn, 60% calories from fat + 300 mg zinc/kg diet), or low-fat diet (CON, 10% calories from fat), for 15 weeks. Results: Compared to CON group mice, the final body weights and adipose tissue weights were significantly increased, while the skeletal muscle weights were significantly decreased in mice belonging to the HF and HF+Zn groups. The HF+Zn group had significantly lower levels of fasting blood glucose concentrations than the HF group. Similarly, zinc supplementation significantly decreased the HF-elevated area under the curve values obtained from the oral glucose tolerance test. Skeletal muscle protein levels of ZIP7 in samples obtained from the HF group were significantly decreased as compared to the CON group. Conversely, the skeletal ZIP7 protein levels in the HF+Zn group were significantly increased as compared to the HF group. Moreover, the protein levels of phosphorylated-AKT and glucose transporter 4 in the skeletal muscle were significantly increased subsequent to zinc supplementation. Conclusion: Our data demonstrates that zinc supplementation up-regulates the skeletal muscle ZIP7 expression, which is associated with improved glucose tolerance in the obesity.

Effects of various metal ions on the gene expression of iron exporter ferroportin-1 in J774 macrophages

Bo-Yeon Park,Jayong Chung 한국영양학회 2008 Nutrition Research and Practice Vol.2 No.4

Macrophages play a key role in iron metabolism by recycling iron through erythrophagocytosis. Ferroportin-1 (FPN1) is a transporter protein that is known to mediate iron export from macrophages. Since divalent metals often interact with iron metabolism, we examined if divalent metals could regulate the expression of FPN1 in macrophages. J774 macrophage cells were treated with copper, manganese, zinc, or cobalt at 10, 50, or 100 μM for 16 to 24 h. Then, FPN1 mRNA and protein levels were determined by quantitative real-time PCR and Western blot analyses, respectively. In addition, effects of divalent metals on FPN1 promoter activity were examined by luciferase reporter assays. Results showed that copper significantly increased FPN1 mRNA levels in a dose-dependent manner. The copper-induced expression of FPN1 mRNA was associated with a corresponding increase in FPN1 protein levels. Also, copper directly stimulated the activity of FPN1 promoter-driven reporter construct. In contrast, manganese and zinc had no effect on the FPN1 gene expression in J774 cells. Interestingly, cobalt treatment in J774 cells decreased FPN1 protein levels without affecting FPN1 mRNA levels. In conclusion, our study results demonstrate that divalent metals differentially regulate FPN1 expression in macrophages and indicate a potential interaction of divalent metals with the FPN1-mediated iron export in macrophages.

Co-supplementation of coenzyme Q and selenium ameliorates the hepatic ferroptosis and the development of non-alcoholic fatty liver disease (NAFLD) in mice

Hyewon Choi,Jiwon Choi,Jayong Chung 한국식품영양과학회 2022 한국식품영양과학회 학술대회발표집 Vol.2022 No.10

한국 청소년의 과일, 채소 섭취빈도와 정신건강: 제10-13차 (2014-2017) 청소년건강행태조사를 이용하여

오지원(Jiwon Oh),정자용(Jayong Chung) 한국영양학회 2020 Journal of Nutrition and Health Vol.53 No.5

본 연구는 2014–2017년도 청소년건강행태조사 원시자료를 이용하여 만 12–18세에 해당하는 남학생 137,101명, 여학생 130,806명을 대상으로 과일과 채소 섭취빈도와 주관적 행복상태, 스트레스 인지, 우울 증상 경험 및 자살 생각과의 관련성을 알아보고자 수행되었다. 연구 결과, 우리나라 청소년의 단 66%가 행복한 편이라고 답하였으며, 2.7명 중 1명은 과도한 스트레스, 4명 중 1명은 우울 증상, 8명 중 1명은 자살 생각을 경험하는 등의 정신건강 문제에 노출되어 있었다. 과일과 채소 섭취빈도는 성별, 나이, 가정경제 수준, 주거 형태, 주관적 학업 성취도, 비만도, 흡연 여부 및 음주 여부에 따라 유의적인 차이가 있었으며, 가당음료, 우유, 패스트푸드 섭취 및 아침 결식 여부와 같은 다른 식습관 요인에 의해서도 유의적인 차이가 있었다. 인구사회학적 특성과 생활습관 변수를 보정한 후 과일과 채소 섭취빈도에 따른 정신건강과의 관계를 살펴본 결과, 남학생과 여학생에서 모두 과일과 채소 섭취가 증가함에 따라 비섭취군 대비 주관적 행복상태에 대한 오즈비는 유의적으로 증가하고, 스트레스 인지, 우울 증상, 자살 생각에 대한 오즈비는 유의적으로 감소하였다. 이러한 관계는 다른 식생활 요인 변수들을 추가로 보정한 후에도 여전히 유의적으로 나타나, 과일과 채소 섭취빈도 증가가 청소년의 긍정적인 정신건강과 관련성이 있는 주요 요인임을 확인하였다. 이상의 연구 결과를 종합해 볼 때, 청소년기 건강한 정신건강을 위해 과일과 채소의 섭취가 중요한 것으로 생각되며, 청소년의 과일과 채소 섭취를 증가시킬 수 있는 효과적인 정책과 교육방안이 개발되어야 할 것으로 생각된다. Purpose: This study examined the association between fruit and vegetable intake and mental health in Korean adolescents. Methods: This study used the data from the 2014-2017 Korea Youth Risk Behavior Survey, a national cross-sectional survey on 137,101 boys and 130,806 girls aged 12-18. Fruit and vegetable intake was assessed based on the frequency of consumption. The outcome variables were the perceived happiness, perceived stress, depressive symptom and suicidal ideation over the previous 12 months. Logistic regression models were used after adjusting for the demographic, life style and other dietary factors. Results: Only 34% and 29% of Korean adolescents consumed fruits more than 5 times/week and vegetables more than 2 times/day, respectively; whereas 37%, 25% and 12.2% of Korean adolescents had perceived stress, depressive symptom and suicidal ideation, respectively. After adjusting for the confounding variables, the greater consumption of fruit and vegetable were all associated with a higher odds of perceived happiness; the adjusted odds ratios (AORs) (95% CI) were 1.53 (1.46-1.60) in boys and 1.82 (1.73-1.90) in girls who consumed fruit ≥ 5 times/week, and 1.65 (1.54-1.76) in boys and 1.62 (1.51-1.72) in girls who consumed vegetable ≥ 2 times/day. In contrast, the consumption of fruit or vegetable were all significantly associated with a lower odds of perceived stress, depressive symptom, and suicidal ideation; the AOR (95% CI) were 0.70 (0.67-0.73), 0.88 (0.84-0.93), and 0.78 (0.73-0.83) in boys who consumed fruit 3-4 times/week, and 0.71 (0.67-0.76), 0.88 (0.81-0.94), and 0.68 (0.62-0.74) in boys who consumed vegetable 5-7 times/week. Similar associations of fruit or vegetable consumption with perceived stress, depressive symptom, or suicidal ideation were found in girls. Conclusion: These findings provide evidence that increasing fruit and vegetable intake is important for better mental health among adolescents.

Cadmium increases ferroportin-1 gene expression in J774 macrophage cells via the production of reactive oxygen species

Bo-yeon Park,Jayong Chung 한국영양학회 2009 Nutrition Research and Practice Vol.3 No.3

Cadmium intoxication has been associated with the dysregulation of iron homeostasis. In the present study, we investigated the effect of cadmium on the expression of ferroportin 1 (FPN1), an important iron transporter protein that is involved in iron release from macrophages. When we incubated cadmium with J774 mouse macrophage cells, FPN1 mRNA levels were significantly increased in a dose- and time-dependent manner. Furthermore, the cadmium-induced FPN1 mRNA expression was associated with increased levels of FPN1 protein. On the other hand, cadmium-mediated FPN1 mRNA induction in J774 cells was completely blocked when cells were co-treated with a transcription inhibitor, acitomycin D. Also, cadmium directly stimulated the activity of the FPN1-promoter driven luciferase reporter, suggesting that the cadmium up-regulates FPN1 gene expression in a transcription-dependent manner. Finally, cadmium exposure to J774 macrophages increased intracellular reactive oxygen species (ROS) levels by ~ 2-fold, compared to untreated controls. When J774 cells were co-treated with antioxidant N-acetylcystein, the cadmium-induced FPN1 mRNA induction was significantly attenuated. In summary, the results of this study clearly demonstrated that cadmium increased FPN1 expression in macrophages through a mechanism that involves ROS production, and suggests another important interaction between iron and cadmium metabolism.