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RISS 인기검색어
- 검색키워드
- 저자 : Jawale, Chetan Vilas (검색결과 17 건)
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Jawale, Chetan V.,Lee, John Hwa American Society for Microbiology 2014 CLINICAL AND VACCINE IMMUNOLOGY Vol.21 No.6
<P>The <I>Escherichia coli</I> heat-labile enterotoxin B subunit (LTB) is a potent vaccine adjuvant. <I>Salmonella enterica</I> serovar Enteritidis ghosts carrying LTB (<I>S</I>. Enteritidis-LTB ghosts) were genetically constructed using a novel plasmid, pJHL187-LTB, designed for the coexpression of the LTB and E lysis proteins. <I>S</I>. Enteritidis-LTB ghosts were characterized using scanning electron microscopy to visualize their transmembrane tunnel structures. The expression of LTB in <I>S</I>. Enteritidis-LTB ghost preparations was confirmed by immunoblot and enzyme-linked immunosorbent assays. The parenteral adjuvant activity of LTB was demonstrated by immunizing chickens with either <I>S</I>. Enteritidis-LTB ghosts or <I>S</I>. Enteritidis ghosts. Chickens were intramuscularly primed at 5 weeks of age and subsequently boosted at 8 weeks of age. In total, 60 chickens were equally divided into three groups (<I>n</I> = 20 for each): group A, nonvaccinated control; group B, immunized with <I>S</I>. Enteritidis-LTB ghosts; and group C, immunized with <I>S</I>. Enteritidis ghosts. Compared with the nonimmunized chickens (group A), the immunized chickens (groups B and C) exhibited increased titers of plasma IgG and intestinal secretory IgA antibodies. The CD3<SUP>+</SUP> CD4<SUP>+</SUP> subpopulation of T cells was also significantly increased in both immunized groups. Among the immunized chickens, those in group B exhibited significantly increased titers of specific plasma IgG and intestinal secretory IgA (sIgA) antibodies compared with those in group C, indicating the immunomodulatory effects of the LTB adjuvant. Furthermore, both immunized groups exhibited decreased bacterial loads in their feces and internal organs. These results indicate that parenteral immunization with <I>S</I>. Enteritidis-LTB ghosts can stimulate superior induction of systemic and mucosal immune responses compared to immunization with <I>S</I>. Enteritidis ghosts alone, thus conferring efficient protection against salmonellosis.</P>
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Tightly regulated bacteriolysis for production of empty Salmonella Enteritidis envelope
Jawale, C.V.,Kim, S.W.,Lee, J.H. Elsevier Scientific Pub. Co 2014 Veterinary microbiology Vol.169 No.3
To avoid leaky expression of the bacterial host-toxic PhiX174 lysis gene E from the λpR promoter, a convergent promoter construct was made in which gene E was placed between a sense λpR promoter and an anti-sense P<SUB>araBAD</SUB> promoter. In the presence of l-arabinose, leaky transcription of lysis gene E at 28<SUP>o</SUP>C from the sense λpR promoter was repressed by an anti-sense RNA simultaneously expressed from the P<SUB>araBAD</SUB> promoter. The stringent repression of lysis gene E in the absence of induction temperature resulted into higher concentration of bacteria in culture suspension, and consequently higher and stable production of a Salmonella Enteritidis (S. Enteritidis) ghost. The immunogenicity of the S. Enteritidis ghost was evaluated by immunizing chickens. Chickens from the immunized group demonstrated a significant increase in the levels of S. Enteritidis-specific plasma IgG, intestinal sIgA, and lymphocyte proliferative response. After virulent S. Enteritidis challenge, the immunized group exhibited decreased bacterial recovery from organs compared with the non-immunized group. Together, these results demonstrate that the stringent molecular control over leaky transcription of lysis gene E enabled the stable production of S. Enteritidis ghost, and immunization with the S. Enteritidis ghost can protect chickens by inducing robust humoral and cellular immune responses.
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Synthetic Route for New (Z)-5-[4-(2-Chloroquinolin-3-yl) Methoxy]benzylidinethiazolidine-2,4-diones
Jawale, Dhanaji V.,Pratap, Umesh R.,Mane, Ramrao A. Korean Chemical Society 2011 Bulletin of the Korean Chemical Society Vol.32 No.7
Synthetic route has been developed for the synthesis of new (Z)-5-[4-(2-chloroquinolin-3-yl) methoxy]benzyl-idinethiazolidine-2,4-diones (6a-h) starting from 2-chloro-3-hydroxymethyl quinolines (2a-h). The hydroxy methyl quinolines on tosylation yielded (3a-h). Condensation of the tosyl intermediates with 4-hydroxy benzaldehydes has been carried in DMF in presence of $K_2CO_3$ and obtained 4-quinolinyl methoxy benzaldehydes (4a-h). Conveniently Knoevenagel condensation of quinolinyl methoxy benzaldehydes (4a-h) and 2, 4-thiazolidinedione (5) has been carried in PEG-400 in presence of L-proline and obtained better yields of the titled compounds (6a-h).
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Jawale, C.V.,Lee, J.H. Pergamon Press 2014 Comparative immunology, microbiology and infectiou Vol.37 No.3
The superior conservation of antigenic determinants on the surface of genetically inactivated bacterial ghosts makes them attractive immunogenic inactivated vaccine candidates. The efficacy of Salmonella Enteritidis (SE) ghost vaccination was evaluated in chickens by characterizing the nature of the adaptive immune response. Chickens from the immunized group demonstrated significant increases in SE-specific plasma IgG, intestinal secretory IgA, and lymphocyte proliferative response. The populations of CD4, CD8, and TCR γδ T-cells in immunized chickens were significantly greater than in the controls. Increased levels of IFN-γ, IL-2, IL-6 and IL-10 were observed in peripheral blood mononuclear cells stimulated with SE specific antigen. After virulent SE challenge, the immune system of immunized chickens was rapidly stimulated, as indicated by significantly increased population of CD4 and CD8 T-cells. Furthermore, the immunized group exhibited decreased challenge strain recovery of the internal organs compared to the non-immunized group. Together, these data indicate that the immunization induced humoral and cell-mediated immunity might be responsible for significant reduction of the virulent challenge strain load in the internal organs of immunized chickens.
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Jawale, Chetan V.,Somsanith, Nithiphonh,Eo, Seong Kug,Park, Sang-Youel,Lee, John Hwa Akademiai Kiado Zrt. 2015 ACTA VETERINARIA HUNGARICA Vol.63 No.4
<P><I>Escherichia coli</I> heat-labile enterotoxin B subunit (LTB) protein is a potent adjuvant. <I>Salmonella</I> Gallinarum ghosts carrying LTB (<I>S</I>. Gallinarum-LTB ghosts) were genetically constructed using a plasmid, pJHL187-LTB, designed for the co-expression of the LTB and E lysis proteins. This study evaluates the immunopotentiating effects of Montanide™ ISA 70 VG on S. Gallinarum-LTB ghost vaccination against fowl typhoid. Five-week-old layer chickens were injected intramuscularly with sterile PBS (non-immunised control, Group A), <I>S</I>. Gallinarum-LTB ghost (Group B) or <I>S</I>. Gallinarum-LTB ghost emulsified with Montanide™ ISA 70 VG adjuvant (Group C). Chickens from both Groups B and C showed significant induction of antigen-specific systemic IgG response compared to controls; in addition, Group C showed enhanced induction of systemic IgG response compared to Group B. We observed significant induction of antigen-specific lymphocyte proliferative response and increased mRNA levels of Th1 cytokines (IFN-γ and IL2) in both Groups B and C. Furthermore, in the challenge experiment with a virulent strain of <I>S</I>. Gallinarum, Group C showed higher survival rates compared with other groups. These results indicate that vaccination with the <I>S</I>. Gallinarum-LTB ghost in combination with Montanide™ ISA 70 VG may enhance the protective immunity against fowl typhoid.</P>
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Chaudhari, Atul A,Jawale, Chetan V,Kim, Sam Woong,Lee, John Hwa BioMed Central 2012 VETERINARY RESEARCH Vol.43 No.-
<P>In order to develop a novel, safe and immunogenic fowl typhoid (FT) vaccine candidate, a <I>Salmonella</I> Gallinarum ghost with controlled expression of the bacteriophage PhiX174 lysis gene <I>E</I> was constructed using pMMP99 plasmid in this study. The formation of the <I>Salmonella</I> Gallinarum ghost with tunnel formation and loss of cytoplasmic contents was observed by scanning electron microscopy and transmission electron microscopy. No viable cells were detectable 24 h after the induction of gene <I>E</I> expression by an increase in temperature from 37 °C to 42 °C. The safety and protective efficacy of the <I>Salmonella</I> Gallinarum ghost vaccine was tested in chickens that were divided into four groups: group A (non-immunized control), group B (orally immunized), group C (subcutaneously immunized) and group D (intramuscularly immunized). The birds were immunized at day 7 of age. None of the immunized animals showed any adverse reactions such as abnormal behavior, mortality, or signs of FT such as anorexia, depression, or diarrhea. These birds were subsequently challenged with a virulent <I>Salmonella</I> Gallinarum strain at 3 weeks post-immunization (wpi). Significant protection against the virulent challenge was observed in all immunized groups based on mortality and post-mortem lesions compared to the non-immunized control group. In addition, immunization with the <I>Salmonella</I> Gallinarum ghosts induced significantly high systemic IgG response in all immunized groups. Among the groups, orally-vaccinated group B showed significantly higher levels of secreted IgA. A potent antigen-specific lymphocyte activation response along with significantly increased percentages of CD4<SUP>+</SUP> and CD8<SUP>+</SUP> T lymphocytes found in all immunized groups clearly indicate the induction of cellular immune responses. Overall, these findings suggest that the newly constructed <I>Salmonella</I> Gallinarum ghost appears to be a safe, highly immunogenic, and efficient non-living bacterial vaccine candidate that protects against FT.</P>
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Nandre, R.M.,Jawale, C.V.,Lee, J.H. Pergamon Press 2013 Comparative immunology, microbiology and infectiou Vol.36 No.5
Escherichia coli heat-labile enterotoxin B subunit (LTB) protein is a potent mucosal adjuvant. In this study, the effect of an attenuated Salmonella secreting LTB protein as an adjuvant strain (JOL1228) for a live Salmonella Enteritidis (SE) vaccine candidate (JOL919) was evaluated. In a single immunization experiment, chickens immunized with a mixture of JOL919 (5 parts) and JOL1228 (1 part) showed enhanced mucosal and cellular immune responses and efficient protection against salmonellosis as compared to those unimmunized control chickens. In further analysis, chickens were primed at one day of age and were boosted at the fifth week of age to prolong immune responses and to maximize the protection efficacy against salmonellosis. The immunized groups B (prime and booster with JOL919), C (prime with JOL919-JOL1228 mixture and booster with JOL919), and D (prime and booster with JOL919-JOL1228 mixture) showed significantly higher humoral and cellular immune responses as compared to those in the unimmunized control group A. In addition, immunized groups C and D showed fewer gross lesions in the liver and spleen and a lower number of SE-positive organs, with the lowest bacterial counts in the SE challenge strain as compared to the control group. These results indicate that SE vaccination with the LTB strain can have an adjuvant effect on the vaccine candidate by enhancing immune responses, and that a prime-boost strategy with the addition of the adjuvant strain can efficiently protect birds against salmonellosis.
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