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Method of DNA Extraction from Pinus rigida Wood Pretreated with Sandpaper
( Jamin Lee ),( Tae-jong Kim ) 한국목재공학회 2018 목재공학 Vol.46 No.4
Species identification of wood provides important information for archaeology, restoration of cultural assets, preventing illegal logging, and more. Wood species are usually identified based on their anatomical features with the use of a microscope. However, this method may not be able to distinguish between anatomically similar species or subspecies. To overcome this problem, wood species need to be identified at the molecular level using DNA sequencing. However, unlike living plant cells, wood is difficult to pulverize using a mortar, and DNA extraction from dried wood is challenging. To solve these problems, we propose a pretreatment method in which wood is pulverized using 60-grit sandpaper and hydrated with water for 2 days. Using this method, we were able to stably amplify the rpoB gene from the extracted DNA of Pinus rigida. In addition, sequence analysis of the rpoB gene revealed six single nucleotide polymorphisms (SNPs), which classified the rpoB sequences in the genus Pinus into five groups. Our data indicate that although these SNPs were not suitable for species identification, they can potentially be used to determine the origin of different wood subspecies or individual samples of wood.
Bioconversion of Marin Wastes into Bioplastic Monomers
Jamin SHIN,Jueun JANG,Yebin JU,You-Kyung LEE,Jeongman SEOL,Soo Rin KIM 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
Sargassum horneri is inedible and invasive species of brown macroalgae that expands and drifts in Spring and Summer, causing damages to the ecosystem of the south coast of Korea, especially Jeju island. The Korean government is preventively eliminating a large amounts of S. horneri biomass before reaching to the coast and treating it in landfills or incineration, but this causes another environmental problem. In the present study, S. honeri biomass was tested as a raw material for the biological production of lactic acid used in various fields such as food additives, cosmetic and chemical industry. Simultaneous saccharification and fermentation (SSF) with lactic acid-producing engineered yeast was optimized in various conditions including type of commercial cellulases, solid loading, and scale-up fermentation. Finally, lactic acid was produced at a yield of 0.24 g lactic acid/g dried biomass and productivity of 0.73 g lactic acid/L-h, suggesting an industrial potential to biologically utilize S. honeri coastal waste.
Development of Clarification Methods for Apple Cider Production from Fresh Apples
Jamin SHIN,Jueun JANG,Soo Rin KIM 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
The apple is one of the antioxidant-rich crops which is widely grown up in the temperate climates, so many processed foods/beverages using apples have been developed. Among beverages the cider and apple-fermented alcoholic beverage, are commonly consumed beverages in Europe and Northern America. However, in the apple extract producing process of apple juice and cider, there is a low extract yield problem because of high pectin and glucan contents in apples. In this study, we made apple hydrolysates by treated pectinase and glucanase and filtrated the hydrolysate of apple extracts through centrifuge and syringe. As a result, through enzyme pre-treatment and treatment time optimizing, we improved the clarification process in apple cider manufacture. In a sensory test, apple cider which is made by the improved process has advantages than old one. In this study, we present optimized clarification process conditions in apple cider production.
A systematic approach towards accident analysis and prevention
Jamin Koo,Seunghyok Kim,Hyosuk Kim,Young-Hun Kim,En Sup Yoon 한국화학공학회 2009 Korean Journal of Chemical Engineering Vol.26 No.6
A systematic approach towards accident analysis and prevention has been developed. It relies on system theory as an incident causation model, and adopts a hybrid model for identifying elements of the safety management system. PDCA (Plan-Do-Check-Act) process, commonly practiced in business for quality control, has been applied to defining components of the system. Using the experts’ judgment, accident data and their reported causes are correlated to the defined components, with RBI (risk-based inspection) defined consequence scores as weighting factors. The application of this approach allows users such as governments and companies to identify and prioritize among causes of accidents and near-misses in the petrochemical industry. A case study using the accident data of Yeosu petrochemical complex from 1990 to 2004 has been applied to illustrate insights readily obtainable by using the developed analysis technique. The results suggest comprehensive identification and ranking of accident causes for effective prevention of accidents in the future.
Robotic Microsurgery Optimization
Jamin V Brahmbhatt,Ahmet Gudeloglu,Philippe Liverneaux,Sijo J Parekattil 대한성형외과학회 2014 Archives of Plastic Surgery Vol.41 No.3
The increased application of the da Vinci robotic platform (Intuitive Surgical Inc.) for microsurgery has led to the development of new adjunctive surgical instrumentation. In microsurgery, the robotic platform can provide high definition 12×−15× digital magnification, broader range of motion, fine instrument handling with decreased tremor, reduced surgeon fatigue, and improved surgical productivity. This paper presents novel adjunctive tools that provide enhanced optical magnification, micro-Doppler sensing of vessels down to a 1-mm size, vein mapping capabilities, hydro-dissection, micro-ablation technology (with minimal thermal spread−CO2 laser technology), and confocal microscopy to provide imaging at a cellular level. Microsurgical outcomes from the use of these tools in the management of patients with infertility and chronic groin and testicular pain are reviewed. All these instruments have been adapted for the robotic console and enhance the robot-assisted microsurgery experience. As the popularity of robot-assisted microsurgery grows, so will its breadth of instrumentation.
Ku, Jamin,Song, Suhee,Park, Sung Heum,Lee, Kwanghee,Suh, Hongsuk,Lansac, Yves,Jang, Yun Hee American Chemical Society 2015 The Journal of Physical Chemistry Part C Vol.119 No.25
<P>A 2,2-dimethyl-2H-benzimidazole (22MBI) pulling unit has been synthesized as a potential high-solubility substitute for benzothiadiazole and incorporated into a push pull-type copolymer used for decent-efficiency (3%) organic photovoltaic devices. We herein replace the two methyl side groups of 22MBI by longer alkyl (ethyl, butyl, and hexyl) side chains to further improve the solubility. However, the copolymers replaced by the new pulling units, 2,2-diethyl/dibutyl/dihexl-2H-benzimidazole (22EBI/22BBI/22HB1), lose favorable optical characteristics and exhibit negligible (<0.5%) power conversion efficiency. Intrigued by this anomalous side-group effect of 2,2-dialkyl-2H-benzimidazole (22BI), we carry out time-dependent density functional theory calculations on a series of 22BI-based copolymers with various lengths of 2,2-dialkyl side chains (methyl, ethyl, butyl, and hexyl), but no discernible difference in equilibrium structure nor in electronic structure is found between them. We hence formulate a hypothesis that 22BI may either isomerize into 1,2-dialkylbenzimidazole (12BI) or lose one of its alkyl (>= ethyl) chains as olefin to become 2-dialkylbenzimidazole (2BI) because these aromatic products, unlike the quinoid-type 22BI, would exhibit unfavorable electronic structure for organic photovoltaic (OPV) applications. Indeed, the absorption spectra measured for the 22BI-based copolymers with long dialkyl side chains are best reproduced by the calculations on 2BI- and 12BI-based copolymers, and the two side products are calculated to be more stable than 22BI, indicating the spontaneity of the proposed reactions. The activation barriers are prohibitively high (>29 kcal/mol) but could be reduced down to 8 kcal/mol in the presence of palladium-based polymerization catalysts. Indeed, the presence of the predicted 12BI-containing side products is confirmed by NMR spectra. A temperature-dependent polymerization experiment shows that 22MBI is in fact subject to the same type of isomerization when the temperature is raised to 150 degrees C above the original polymerization temperature (90-110 degrees C), further supporting the hypothesis from our calculations and explaining the observed anomalous side-group effect.</P>