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Asjad Ali,Eun Mi Yang,Sun Woong Bang,Sang-Min Chung,Jack E. Staub 한국원예학회 2014 원예과학기술지 Vol.32 No.2
The responses to chilling temperature of 12 Korean cucumber varieties was compared to those of two U.S.A. (previously determined cold tolerant NC76 and ‘Chipper’), and Chinese and Japanese germplasms. Seedlings of each entry were exposed to 4°C (Experiment 1) and 1°C (Experiments 2 and 3) at the first-true leaf stage for eight and nine hours, respectively, under 80% relative humidity (RH) and 149 μmoles・m<SUP>-2</SUP>・s<SUP>-1</SUP> photosynthetic photon flux (PPF). The chilling response [damage rating (DR)] of each accession was based on visual ratings (1 to 5) after treatment, where 1 = no damage, 2 = slight, 3 = moderate, 4 = advanced, and 5 = severe damage. Predictably the cumulative average DR of chilling tolerant line NC76 and ‘Chipper’ after chilling w as 1 a nd 1 .1, respectively . Korean ‘Nacdongchungjang’ w as m ost sensitive to c hilling temperatures [DR = 2 .3] when compared to the other entries examined. The sensitivity to chilling of ‘Nacdongchungjang’ was followed by Chinese ‘Dongguan’ [DR = 1.7]. In contrast, ‘Saeronchungjang’ (DR = 1) and ‘Janghyungnachap’ (DR = 1) were the most chilling tolerant of the Korean accessions examined and equivalent to the response of line NC76 and ‘Chipper’. Nevertheless, chloroplast type genotyping of these accessions with known chilling-linked sdCAPS genomic markers revealed genotypic differences between chilling tolerant lines (NC76 and ‘Chipper’) and all Korean lines examined.
A method for selection of restriction enzymes for sdCAPS marker construction
Jeong, YeSol,Lee, SunYoung,Choi, InHye,Lim, YongPyo,Hur, YoonKang,Staub, Jack E.,Chung, SangMin Blackwell Publishing Ltd 2011 Plant breeding Vol.130 No.3
<P>With 2 figures</P><P><B>Abstract</B></P><P>Development of PCR‐based markers for single‐nucleotide polymorphism (SNP) detection is prerequisite for various genetic analyses. The use of restriction enzymes (REs) following PCR amplification is a common and relatively low cost method for SNP detection. Simple and cost‐effective methodologies for SNP marker development that would enhance the use of SNP‐based technologies are desirable. As an alternative analytical method for selection of REs for recognition of SNP motifs for marker development that does not require computer‐based selection of REs is herein described. Given that only 12 REs are required for the detection of any SNP motif, the method described in this study is relatively inexpensive and technically simple.</P>
Ali, Asjad,Yang, Eun Mi,Bang, Sun Woong,Chung, Sang-Min,Staub, Jack E. Korean Society of Horticultural Science 2014 원예과학기술지 Vol.32 No.2
The responses to chilling temperature of 12 Korean cucumber varieties was compared to those of two U.S.A. (previously determined cold tolerant NC76 and 'Chipper'), and Chinese and Japanese germplasms. Seedlings of each entry were exposed to $4^{\circ}C$ (Experiment 1) and $1^{\circ}C$ (Experiments 2 and 3) at the first-true leaf stage for eight and nine hours, respectively, under 80% relative humidity (RH) and $149{\mu}moles{\cdot}m^{-2}{\cdot}s^{-1}$ photosynthetic photon flux (PPF). The chilling response [damage rating (DR)] of each accession was based on visual ratings (1 to 5) after treatment, where 1 = no damage, 2 = slight, 3 = moderate, 4 = advanced, and 5 = severe damage. Predictably the cumulative average DR of chilling tolerant line NC76 and 'Chipper' after chilling w as 1 and 1.1, respectively. Korean 'Nacdongchungjang' was most sensitive to chilling temperatures [DR = 2.3] when compared to the other entries examined. The sensitivity to chilling of 'Nacdongchungjang' was followed by Chinese 'Dongguan' [DR = 1.7]. In contrast, 'Saeronchungjang' (DR = 1) and 'Janghyungnachap' (DR = 1) were the most chilling tolerant of the Korean accessions examined and equivalent to the response of line NC76 and 'Chipper'. Nevertheless, chloroplast type genotyping of these accessions with known chilling-linked sdCAPS genomic markers revealed genotypic differences between chilling tolerant lines (NC76 and 'Chipper') and all Korean lines examined.