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Kim, Hyunji,Park, MinSeok,Park, Songyi,Jeong, Hoiwoun,Kim, Jungmin,Kim, Youhyun Nuclear Technology Pub 2013 Radiation protection dosimetry Vol.153 No.1
<P>With the introduction of digital radiography, patients undergoing radiographic procedures are subject to being overexposed to radiation. Therefore, it is necessary to estimate the absorbed organ dose and the effective dose, which are significant for patient health, along with body type. During chest radiographic examinations conducted in 899 patients for screening, the absorbed dose of the 13 major organs, the average whole-body dose, and two effective doses weighted by factors published in ICRP 60 and ICRP 103 were calculated on the basis of patient information such as height, weight and examination condition, including kilovolt potential, focus-skin distance and entrance surface dose (ESD), using a PC-based Monte Carlo program simulation. It was found that dose per unit ESD had a tendency to decrease with body mass index (BMI). In particular, the absorbed dose for most organs was larger at high voltages (140 kVp) than at low voltages (120 kVp, 100 kVp). In addition, the effective dose which was based on ICRP 60 and ICRP 103 also represented the same tendency in respect of BMI and tube voltage.</P>
Generation of Integration-free Induced Neural Stem Cells from Mouse Fibroblasts
Kim, Sung Min,Kim, Jong-Wan,Kwak, Tae Hwan,Park, Sang Woong,Kim, Kee-Pyo,Park, Hyunji,Lim, Kyung Tae,Kang, Kyuree,Kim, Jonghun,Yang, Ji Hun,Han, Heonjong,Lee, Insuk,Hyun, Jung Keun,Bae, Young Min,Scho American Society for Biochemistry and Molecular Bi 2016 The Journal of biological chemistry Vol.291 No.27
<P>The viral vector-mediated overexpression of the defined transcription factors, Brn4/Pou3f4, Sox2, Klf4, and c-Myc (BSKM), could induce the direct conversion of somatic fibroblasts into induced neural stem cells (iNSCs). However, viral vectors may be randomly integrated into the host genome thereby increasing the risk for undesired genotoxicity, mutagenesis, and tumor formation. Here we describe the generation of integration-free iNSCs from mouse fibroblasts by non-viral episomal vectors containing BSKM. The episomal vector-derived iNSCs (e-iNSCs) closely resemble control NSCs, and iNSCs generated by retrovirus (r-iNSCs) in morphology, gene expression profile, epigenetic status, and self-renewal capacity. The e-iNSCs are functionally mature, as they could differentiate into all the neuronal cell types both in vitro and in vivo. Our study provides a novel concept for generating functional iNSCs using a non-viral, non-integrating, plasmid-based system that could facilitate their biomedical applicability.</P>
HSPF의 surface FTABLE 기능을 활용한 저영향개발 기법 적용에 따른 수문요소 분석
이현지 ( Hyunji Lee ),강문성 ( Moon Seong Kang ),김학관 ( Hakkwan Kim ),김지혜 ( Jihye Kim ),김석현 ( Seokhyeon Kim ),곽지혜 ( Jihye Kwak ),김시내 ( Sinae Kim ) 한국농공학회 2022 한국농공학회 학술대회초록집 Vol.2022 No.-
도시화로 인해 유역의 불투수면적이 증가하였으며 이는 유역의 수문학적 요소에 큰 변화를 가져왔다. 이러한 증가된 불투수면적이 강우유출수의 수문에 미치는 영향을 완화하기 위해 최근 저영향개발(Low Impact Development; LID) 기법이 개발되어 적용되고 있다. LID 기법은 개발 이전의 수문순환 상태에 최대한 근사하도록 개발하는 기법으로 우수 유출 속도 감소, 유역에서의 저류, 침투 및 증발산 과정을 촉진시킨다. 효과적인 저영향개발 기법 적용을 위해서는 시설 설치 전 모형을 이용한 저영향개발 기법의 적용 효과를 파악해야 한다. 이에 따라 많은 저영향개발 기법 모형들이 개발되어 적용되고 있다. 기존 HSPF (Hydrological Simulation Program: FORTRAN) 모형 내 LID tool을 이용하여 LID 기법 적용 시 LID 시설을 통한 침투량이 유역의 중간유출이나 기저유출에 기여하지 못하는 문제가 있었다. 이에 본 연구에서는 HSPF 12.4 버전에 추가된 surface FTABLE 기능을 활용하여 LID 기법의 저류현상을 구현하고 기존의 LID tool을 활용한 방법과의 수문요소에 대한 영향을 분석하였다. HSPF 모형의 입력자료를 구축하여 모형을 구축하고 LID 기법 적용 전에 모형의 보정 및 검정을 수행하였으며, LID 기법을 적용하여 유역 내 수문요소들의 변화를 분석하였다. 지표유출의 경우 두 방법에서 모두 유사하게 감소하였다. 기존의 LID tool을 활용한 방법에서는 중간유출과 기저유출이 LID 기법을 적용하지 않은 경우와 유사한 값을 보이며 물수지 분석에서 LID 기법을 적용하지 않은 경우와 큰 차이가 발생하였다. 그러나 surface FTABLE을 활용한 방법에서는 LID 시설을 통한 침투량이 중간유출과 기저유출에 기여하여 그 값이 증가한 것을 확인할 수 있었으며 물수지 분석에서도 LID 기법을 적용하지 않은 경우와 유사한 값을 보였다. 본 연구의 결과는 향후 LID 효과분석을 위해 활용할 수 있을 것으로 사료된다.
Adenine base editing in mouse embryos and an adult mouse model of Duchenne muscular dystrophy
Ryu, Seuk-Min,Koo, Taeyoung,Kim, Kyoungmi,Lim, Kayeong,Baek, Gayoung,Kim, Sang-Tae,Kim, Heon Seok,Kim, Da-eun,Lee, Hyunji,Chung, Eugene,Kim, Jin-Soo Nature Publishing Group, a division of Macmillan P 2018 Nature biotechnology Vol.36 No.6
<P>Adenine base editors (ABEs) composed of an engineered adenine deaminase and the Streptococcus pyogenes Cas9 nickase enable adenine-to-guanine (A-to-G) single-nucleotide substitutions in a guide RNA (gRNA)-dependent manner. Here we demonstrate application of this technology in mouse embryos and adult mice. We also show that long gRNAs enable adenine editing at positions one or two bases upstream of the window that is accessible with standard single guide RNAs (sgRNAs). We introduced the Himalayan point mutation in the Tyr gene by microinjecting ABE mRNA and an extended gRNA into mouse embryos, obtaining Tyr mutant mice with an albino phenotype. Furthermore, we delivered the split ABE gene, using trans-splicing adenoassociated viral vectors, to muscle cells in a mouse model of Duchenne muscular dystrophy to correct a nonsense mutation in the Dmd gene, demonstrating the therapeutic potential of base editing in adult animals.</P>
Lee, Jaemin,Kang, Tae Heung,Yoo, Wonbeak,Choi, Hyunji,Jo, Seongyea,Kong, Kyungsu,Lee, Sang-Rae,Kim, Sun-Uk,Kim, Ji-Su,Cho, Duck,Kim, Janghwan,Kim, Jeong-Yoon,Kwon, Eun-Soo,Kim, Seokho AMERICAN ASSOCIATION FOR CANCER RESEARCH 2019 CANCER IMMUNOLOGY RESEARCH Vol.7 No.2
<P>The homing of natural killer (NK) cells is often inhibited by pancreatic cancer tumors. A mesothelin-directed antibody conjugated to a cleavable NK cell—recruiting chemokine increased NK-cell infiltration of PDAC tumors, reduced tumor burden, and improved survival.</P><P>Natural killer (NK) cells are primary immune cells that target cancer cells and can be used as a therapeutic agent against pancreatic cancer. Despite the usefulness of NK cells, NK-cell therapy is limited by tumor cell inhibition of NK-cell homing to tumor sites, thereby preventing a sustained antitumor immune response. One approach to successful cancer immunotherapy is to increase trafficking of NK cells to tumor tissues. Here, we developed an antibody-based NK-cell–homing protein, named NK-cell–recruiting protein-conjugated antibody (NRP-body). The effect of NRP-body on infiltration of NK cells into primary and metastatic pancreatic cancer was evaluated <I>in vitro</I> and in murine pancreatic ductal adenocarcinoma models. The NRP-body increased NK-cell infiltration of tumors along a CXCL16 gradient (CXCL16 is cleaved from the NRP-body by furin expressed on the surface of pancreatic cancer cells). CXCL16 induced NK-cell infiltration by activating RhoA via the ERK signaling cascade. Administration of the NRP-body to pancreatic cancer model mice increased tumor tissue infiltration of transferred NK cells and reduced the tumor burden compared with that in controls. Overall survival of NRP-body–treated mice (even the metastasis models) was higher than that of mice receiving NK cells alone. In conclusion, increasing NK-cell infiltration into tumor tissues improved response to this cancer immunotherapy. The combination of an NRP-body with NK-cell therapy might be useful for treating pancreatic cancer.</P>