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Hwang, You-Jin,Yang, Jae-Hun,Kim, Sang-Ok,Kim, Bo-Kyung,Choi, Seon-Kyu,Park, Choon-Keun,Kim, Dae-Young 韓國受精卵移植學會 2009 한국동물생명공학회지 Vol.24 No.4
The beneficial effect of glycerol as a cryoprotectant, especially for sperm cryopreservation, has been shown in many studies. However, glycerol is toxic to living cells, and boar sperm in particular show greater sensitivity to glycerol than sperm from other domestic animals. Amides have been studied as alternative cryoprotectants for freezing stallion sperm. Sperm frozen in methylformamide or dimethylformamide as cryoprotectants show similar motility when thawed compared with sperm frozen in glycerol. We evaluated the cryoprotective effects of dimethylformamide on boar sperm freezing. To test the effect of amides, the concentration of boar semen was adjusted to , and seminal plasma was removed using Hulsen solution. After centrifugation, the pellet was diluted in modified-Modena B extender. Lactose-egg yolk (LEY) extender was used as the cooling extender. The freezing extender was madeed aaddition of the optimal amount of glycerol and amides to LEY-Glycerol-Orvus ES Paste extender, and this extender was used for the second dilution. Diluted sperm were frozen in liquid nitrogen using the 0.5 mL straw method. Sperm frozen in extender with glycerol as a cderol were compared with those frozen in extender including the different amides. Sperm were tested for motility, viability, the sperm chromatin structure assay, and normal apical ridge after thawing. The percent of motile sperm diluted in glycerol was as high as that in the stallion study (61%). Dimethylformamide showed positive effects on sperm quality and was better than glycerol. Methylformamide provided similar sperm quality as glycerol. Therefore, dimethylformamide is useful for reducing cryoinjury in boar sperm and is expected to be useful as an alternative cryoprotectant.
THE EFFECTS OF CAPACITY RATIO ON THE PERFORMANCE OF ALKALINE BATTERIES
Hwang, Sung Min,Kim, Jae Geon,Kim, Sun Geon Kim,Yeu, Tae Whan 한국화학공학회 1995 Korean Journal of Chemical Engineering Vol.12 No.2
The effects of cathode/anode capacity ratio on the performance of mercury-free alkaline zinc-manganese dioxide batteries were studied. An alternating current impedance analysis and a continuous discharge test with constant electrical load were employed. The discharge and leakage performances of alkaline batteries varied strongly with capacity ratios and discharge rates. The lower capacity ratios favored discharge performance at high rates, whereas discharge performance at low rates depended only on the quantity of manganese dioxide present. In addition, the lower capacity ratios yielded high in-cell gassing with severe leakage problems, especially at partially discharged cell.
Hwang, Yo-Sep,Bang, Seok Jin,Kang, Tae Yun,Choi, Jae Hyeok,Jung, Sang Mok,Kang, In Sung,Jeon, Se young,Park, Kwan Ha,Choi, Sanghoon The Korean Society of Fish Pathology 2019 한국어병학회지 Vol.32 No.1
The study investigated the dietary effects of medicinal herbs extract and multiple probiotics mixture on the growth performance, innate immune response and antibacterial activity of nile tilapia Oreochromis niloticus. Tilapia were divided in four groups. The first is a fish group fed a basal diet added with 40% medicinal herbs extract (MHE). The second is a fish group fed a basal diet supplied with $2{\times}10^8CFU/g$ of 2 Bacillus sp, 2 Lactobacillus sp and 2 Yeast sp, respectively (PB). The third group was fed with a mixture of probiotics (2 Bacillus sp, 2 Lactobacillus sp and 2 Yeast sp) with the medicinal herbs extract added in basal diet (MHE+PB). The fourth group was fed only a basal diet (C). In a non-specific immune parameters analysis, respiratory burst activity, lysozyme activity, phagocytic activity (PA), alternative complement pathway activity ($ACH_{50}$) and superoxide dismutase (SOD) activity were significantly (p<0.05) increased in the group MHE+PB compared to other groups. Both PB and MHE groups showed a significant (p<0.05) increase in respiratory burst activity, lysozyme activity compared to the control C group, whereas no significant differences were observed in PA, $ACH_{50}$ and SOD activity compared to the control group. In challenging test, fish were administered with Edwardsiella tarda (E. tarda) on 30 days after feeding with each experimental diet and viable E. tarda cell reduction was checked over 21 days post injection. MHE+PB group showed a significantly (p<0.05) reduced E. tarda cells compared to other groups. No significant antibacterial difference (p>0.05) was observed between PB and MHE only treated group. Compared to the control, a significant antibacterial difference (p<0.05) appeared in PB but not in MHE (p>0.05). The results suggest that the probiotics and MHE mixture could be utilized as an alternative to antibiotics in the control of fish diseases caused by E. tarda.
Space Radiation Measurement on the Polar Route onboard the Korean Commercial Flights
Hwang, Jung-A,Lee, Jae-Jin,Cho, Kyung-Suk,Choi, Ho-Sung,Rho, Su-Ryun,Cho, Il-Hyun The Korean Space Science Society 2010 Journal of Astronomy and Space Sciences Vol.27 No.1
This study was performed by the policy research project of Ministry of Land, Transport and Maritime Affairs, which title is "Developing safety standards and management of space radiation on the polar route". In this research, total six experiments were performed using Korean commercial flights (B747). Three of those are on the polar route and the other three are on the north pacific route. Space radiation exposure measured on the polar route is the average 84.7 uSv. The simulation result using CARI-6M program gives 84.9 uSv, which is very similar to measured value. For the departure flight using the north pacific route, the measured space radiation is the average 74.4 uSv. It seems that is not so different to use the polar route or not for the return flight because the higher latitude effect causing the increase of space radiation is compensated by the shortened flight time effect causing decreasing space radiation exposure.
Sex Ratio Determination by Quantitative Real Time PCR using Amelogenin Gene in Porcine Sperm
Hwang, You-Jin,Bae, Mun-Sook,Yang, Jae-Hun,Kim, Bo-Kyoung,Kim, Sang-Ok,Lee, Eun-Soo,Choi, Sun-Gyu,Kwon, Ye-Ri,Seo, Min-Hae,Park, Choon-Keun 韓國受精卵移植學會 2009 한국동물생명공학회지 Vol.24 No.3
Sex-sorting of sperm is an assisted reproductive technology (ART) used by the livestock industry for the mass production of animals of a desired sex. The standard method for sorting sperm is the detection of DNA content differences between X and Y chromosome-bearing sperm by flow cytometry. However, this method has variable efficiency and therefore requires verification by a second method. We have developed a sex determination method based on quantitative real-time polymerase chain reaction (qPCR) of the porcine amelogenin (AMEL) gene. The AMEL gene is present on both the X and the Y chromosome, but the length and sequence of its noncoding regions differ between the X and Y chromosomes. By measuring the threshold cycle (Ct) of qPCR, we were able to calculate the relative frequency of X chromosome. Two sets of AMEL primers were used in these studies. One set (AME) targeted AMEL gene sequences present in both X and Y chromosome, but produced PCR products of different lengths for each chromosome. The other set (AXR) bound to AMEL gene sequences present on the X chromosome but absent esholthe Y-chromosome. Relative product levels were calculated by normalizing the AXR fluorescence to the AME fluorescence. The AMEL method accurately predicted the sex ratios of boar sperm, demonstrating that it has potential value as a sex determination method.
Effects of oxygen pressure on the crystalline of ZnO lms grown on sapphire by PLD method
Jae Hyung Jo,Hyung Kook Kim,Dae Young Kwon,Jin Sung Kwak,Tae-Bong Hur,Yoon-Hwae Hwang 한국물리학회 2005 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.47 No.2
We studied the effects of oxygen pressure on the crystallinity of ZnO films. ZnO films on sapphire(001) substrates were deposited by the pulsed laser deposition (PLD) technique and were grown at various oxygen pressures ranging from 5 mTorr to 150 mTorr to investigate the effect of ambient O2 pressure on the structural properties of the films. We found that the lattice constant along the c-axis and the crystallinity of ZnO films were sensitive to the O2 pressure.
Sang Jin Lee,Jae Sung Lee,Kwang-Jin Hwang,Young Kwan Kim,Young Sik Kim,Noh Gill Park,Eun Ju Shin,Seung Hee Lee 한국물리학회 2005 Current Applied Physics Vol.5 No.1
The preparation and photophysics of organometallic Ir(III) complexes with 3-phenylpyridazine (H6Clppdz) and its derivative arereported. IrCl3 ÆnH2O reacts with H6Clppdz, and 3-chloro-6-(30-methoxyphenyl)-4-methyl-pyridazine (MCOMppdz) to give thecorresponding chloride-bridged dimers, (6Clppdz)2Ir(l -Cl)2Ir(6Clppdz)2 and (MCOMppdz) 2Ir(l -Cl)2Ir(MCOMppdz)2 in goodyields. These chloride-bridged dimers are cleaved with 2-pyrazinecarboxylic acid (Hpyzc), 2-picolinic acid (Hpic), acetylacetone(Hacac), and 2,2,6,6-tetramethyl-3,5-heptanedione (Htmd) to give the corresponding monomer, (6Clppdz)2Ir(pyzc), (6Clppdz)2-Ir(pic), (6Clppdz)2Ir(acac), (6Clppdz)2Ir(tmd), (MCOMppdz)2Ir(acac), and (MCOMppdz)2Ir(tmd), respectively. The complexesshow bright orange to red luminescence at room temperature and the emission wavelenghts are aected by the ancillary ligands aswell as cyclometalating ligands (593664 nm).