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- 저자 : Huchen Chen (검색결과 5 건)
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Chen, Huchen,Zhang, Shuhan,He, Shengnan,A, Runa,Wang, Mingyang,Liu, Shouan The Korean Society of Ginseng 2022 Journal of Ginseng Research Vol.46 No.6
Background: Panax ginseng Meyer is one of the most valuable medicinal plants which is enriched in anti-microbe secondary metabolites and widely used in traditional medicine. Botrytis cinerea is a necrotrophic fungus that causes gray mold disease in a broad range of hosts. B. cinerea could overcome the ginseng defense and cause serious leaf and root diseases with unknown mechanism. Methods: We conducted simultaneous transcriptomic and metabolomic analysis of the host to investigate the defense response of ginseng affected by B. cinerea. The gene deletion and replacement were then performed to study the pathogenic gene in B. cinerea during ginseng - fungi interaction. Results: Upon B. cinerea infection, ginseng defense responses were switched from the activation to repression, thus the expression of many defense genes decreased and the biosynthesis of antifungal metabolites were reduced. Particularly, ginseng metabolites like kaempferol, quercetin and luteolin which could inhibit fungi growth were decreased after B. cinerea infection. B. cinerea quercetin dioxygenase (Qdo) involved in catalyzing flavonoids degradation and ∆BcQdo mutants showed increased substrates accumulation and reduced disease development. Conclusion: This work indicates the flavonoids play a role in ginseng defense and BcQdo involves in B. cinerea virulence towards the P. ginseng. B. cinerea promotes disease development in ginseng by suppressing of defense related genes expression and reduction of antifungal metabolites biosynthesis.
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Botrytis cinerea hypovirulent strain BcSpd1 induced Panax ginseng defense
Shuhan Zhang,Junyou Han,Ning Liu,Jingyuan Sun,Huchen Chen,Jinglin Xia,Huiyan Ju,Shouan Liu 고려인삼학회 2023 Journal of Ginseng Research Vol.47 No.6
Background: Gray mold, caused by Botrytis cinerea, is one of the major fungal diseases in agriculture. Biological methods are preferred over chemical fungicides to control gray mold since they are less toxicto the environment and could induce the resistance to pathogens in plants. In this work, we try tounderstand if ginseng defense to B. cinerea could be induced by fungal hypovirulent strain △BcSpd1. BcSpd1 encodes Zn(II)2Cys6 transcription factor which regulates fungal pathogenicity and we recentlyreported △BcSpd1 mutants reduced fungal virulence. Methods: We performed transcriptomic analysis of the host to investigate the induced defense responseof ginseng treated by B. cinerea △BcSpd1. The metabolites in ginseng flavonoids pathway were determinedby UPLC-ESI-MS/MS and the antifungal activates were then performed. Results: We found that △BcSpd1 enhanced the ginseng defense response when applied to healthyginseng leaves and further changed the metabolism of flavonoids. Compared with untreated plants, theapplication of △BcSpd1 on ginseng leaves significantly increased the accumulation of p-coumaric acidand myricetin, which could inhibit the fungal growth. Conclusion: B. cinerea△BcSpd1 could effectively induce the medicinal plant defense and is referred to asthe biological control agent in ginseng disease management.
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Efficient Driving of Tracking Robot Using Kalman Filters
Hu Chen,Hyun-seop Lim,Seung-ik Hwang,Jang-myung Lee 제어로봇시스템학회 2013 제어로봇시스템학회 국제학술대회 논문집 Vol.2013 No.10
Path planning of mobile robot has a purpose to design an optimal path from initial position to target point. Driving distance is considered when choose the optimal path. In this paper, we proposed efficient driving trajectory when mobile robot follows moving object in real time. First, position and distance of moving object can be acquired using one web camera and rotation angular velocity, linear velocity is estimated using Kalman filter to predict trajectory of moving object. Finally mobile robot follows moving object using proposed tracking control method by estimating trajectory of moving object.
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HU CHEN,YUNFEI LIU,YINONG LU,HONGMING WU,HAO QIAN,BAOSHUAI HUANG 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2014 NANO Vol.9 No.4
The Eu3+-doped CdTe quantum dots (QDs) have been successfully synthesized in aqueous phase through microwave-assisted hydrothermal method. The as-prepared products were characterized by X-ray diffraction (XRD) technique, high-resolution transmission electron microscope (HRTEM), energy dispersive spectrum (EDS), X-ray photoclectron spectrum (XPS), UV-visble (UV-Vis) absorption spectrum and photoluminescence (PL). As the results show, the average sizes of the cubic phase CdTe and CdTe:1%Eu3+ are an average size of 3.7 nm and 3.5 nm, respectively. The average lattice parameter of CdTe:Eu3+ decreases with increasing Eu3+ ions concentration. Furthermore, compared to green emission of the host CdTe, the CdTe:1%Eu3+ presents additional orange fluorescence emission of Eu3+ ions. Recombination luminescence color is almost located in the yellow region of the CIE 1931, and the CIE 1931 coordinate is (0.448, 0.513). The effect of different Eu3+ doping concentration on the luminescence of CdTe:Eu3+ QDs was discussed in detail. It suggests that CdTe:Eu3+ QDs are potential phosphors for white light-emitting materials.
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Path Planning of 5-DOF Manipulator Based on Maximum Mobility
Hu Chen,이장명 한국정밀공학회 2014 International Journal of Precision Engineering and Vol.15 No.1
In this paper, a new path-planning algorithm is proposed based on inverse kinematics (IK) and end effector maximum mobility direction. As a fundamental tool, the IK is used in many fields, forexample, path planning. In order to find the manipulator configuration, first, a 5-DOF humanoid manipulator IK solver is obtained and verified. According to Yoshikawa’s manipulability definition, the mobility of the end effector in a desired direction can be measured. If the end effector moves along the maximum mobility direction, the motion generation efficiency in the Cartesian space is optimal. However, the maximum mobility direction is defined locally for the current configuration of the manipulator. If the start configuration and the traveling time are pre-determined, the path can be determined uniquely, and the end effector may not reach the precise goal position. Therefore, a unit direction vector is used for revising the direction and making the end effector move close to the goal position. The simulation results demonstrate the effectiveness of this path-planning algorithm.
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