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Shirazy, Najmeh Hadizadeh,Ranjbar, Bijan,Hosseinkhani, Saman,Khalifeh, Khosrow,Madvar, Ali Riahi,Naderi-Manesh, Hossein Korean Society for Biochemistry and Molecular Biol 2007 Journal of biochemistry and molecular biology Vol.40 No.4
Bacterial luciferase is a heterodimeric enzyme, which catalyzes the light emission reaction, utilizing reduced FMN (FMNH2), a long chain aliphatic aldehyde and $O_2$, to produce green-blue light. This enzyme can be readily classed as slow or fast decay based on their rate of luminescence decay in a single turnover. Mutation of Glu175 in $\alpha$ subunit to Gly converted slow decay Xenorhabdus Luminescence luciferase to fast decay one. The following studies revealed that changing the luciferase flexibility and lake of Glu-flavin interactions are responsible for the unusual kinetic properties of mutant enzyme. Optical and thermodynamics studies have caused a decrease in free energy and anisotropy of mutant enzyme. Moreover, the role of Glu175 in transition state of folding pathway by use of stopped-flow fluorescence technique has been studied which suggesting that Glu175 is not involved in transition state of folding and appears as surface residue of the nucleus or as a member of one of a few alternative folding nuclei. These results suggest that mutation of Glu175 to Gly extended the structure of Xenorhabdus Luminescence luciferase, locally.
Mehdi Mohammadimehr,Mojtaba Mehrabi,Hasan Hadizadeh,Hossein Hadizadeh 국제구조공학회 2018 Steel and Composite Structures, An International J Vol.26 No.4
In this article, static, buckling and free vibration analyses of a sinusoidal micro composite beam reinforced by single-walled carbon nanotubes (SWCNTs) with considering temperature-dependent material properties embedded in an elastic medium in the presence of magnetic field under transverse uniform load are presented. This system is used at micro or sub micro scales to enhance the stiffness of micro composite structures such as bar, beam, plate and shell. In the present work, the size dependent effects based on surface stress effect and modified strain gradient theory (MSGT) are considered. The generalized rule of mixture is employed to predict temperature-dependent mechanical and thermal properties of micro composite beam. Then, the governing equations of motions are derived using Hamilton's principle and energy method. Numerical results are presented to investigate the influences of material length scale parameters, elastic foundation, composite fiber angle, magnetic intensity, temperature changes and carbon nanotubes volume fraction on the bending, buckling and free vibration behaviors of micro composite beam. There is a good agreement between the obtained results by this research and the literature results. The obtained results of this study demonstrate that the magnetic intensity, temperature changes, and two parameters elastic foundations have important effects on micro composite stiffness, while the magnetic field has greater effects on the bending, buckling and free vibration responses of micro composite beams. Moreover, it is shown that the effects of surface layers are important, and observed that the changes of carbon nanotubes volume fraction, beam length-to-thickness ratio and material length scale parameter have noticeable effects on the maximum deflection, critical buckling load and natural frequencies of micro composite beams.
Elham Khodaverdi,Melika Javan,Sayyed A. Sajadi Tabassi,Bahman Khameneh,Hossein Kamali,Farzin Hadizadeh 한국약제학회 2017 Journal of Pharmaceutical Investigation Vol.47 No.3
Formulating a polypeptide gastrointestinal drug delivery system have been persistent challenges. To overcome these challenges, in situ-forming gels are more attractive because of their biodegradability and easy preparation and administration. In this study, biodegradable triblock copolymer polycaprolactone–polyethylene glycol–polycaprolactone [PCL–PEG–PCL (PCEC)] was synthesized under microwave irradiation and their gelation with different concentrations of a-cyclodextrin (CD) was investigated. PCEC was characterized by 1HNMR and gel permeation chromatography. The hydrogel and copolymer were also evaluated using differential scanning colorimetery, X-ray diffraction study and scanning electron microscopy. Also rheological properties were investigated. Four different concentrations of hydrogels consisting of the copolymer [5 and 7.5% (w/w)] and a-CD [10 and 14% (w/ w)] were studied to evaluate insulin cumulative release profiles during 37 days. Finally, CD spectrum and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDSPAGE) tests were performed to approve the stability of released insulin. It was demonstrated that the synthesis of PCEC via microwave irradiation was fast and efficient. Copolymer and a-CD concentrations are important in forming supramolecular hydrogels. Rheographs indicated the injectability and thixotropic behavior. In vitro release studies affirmed the sustained release profile of insulin. Both polymer degradation and drug diffusion are involved in it. Results of stability tests confirmed the stability of insulin following release.