초임계 유체를 이용한 두 종류의 항암제가 함유된 고분자 미립자 제조

유종훈 水原大學校 2012 論文集 Vol.26 No.-

Doxorubicin (DOX) is an anthracycline drug commonly used in the treatment of a large spectrum of tumors. It works by interfering with the growth of cancer cells. It binds and intercalates into the DNA strand, thus inhibiting further DNA and RNA biosynthesis, eventually causing cell death. Gemcitabine (GEM) is a synthetic pyrimidine nucleoside analogue with a high antitumor efficacy for ovarian, breast, bladder, and pancreatic cancer. It replaces one of the building blocks of nucleic acids, in this case cytidine, during DNA replication. The process arrests tumor growth, as new nucleosides cannot be attached to the faulty nucleoside, resulting in apoptosis. The purpose of this study is to prepare biodegradable poly(L-lactic acid) microparticles containing two different drugs by a supercritical aerosol solvent extraction system (ASES) process. The effects of operating parameters such as temperature, pressure, and drug composition on the morphology and characteristics of microparticles were studied in detail. The entrapment efficiency and in vitro release profiles of two anticancer drugs from the microparticles were also determined using HPLC. The entrapment efficiency increased with decreasing temperature. The entrapment efficiency of DOX was found to be higher than that of GEM, mainly due to larger molecular weight of DOX. On the other hand, GEM showed higher solubility in aqueous solution, thereby resulting in a faster release compared with DOX.

감염 조절용 차단막의 두께가 광중합기의 중합광에 미치는 영향

장훈상,이석련,홍성옥,류현욱,송창규,민경산 大韓齒科保存學會 2010 Restorative Dentistry & Endodontics Vol.35 No.5

연구목적: 본 연구는 감염 조절용 차단막을 여러 겹으로 사용했을 때 광중합기의 광강도와 파장, light diffusion 등에 미치는 영향에 대해 조사하였다. 연구 재료 및 방법: 감염 조절용 차단막은 투명 랩 (크린랩)을 사용하였고 광중합기는 할로겐 광중합기 (Optilux 360)와 LED 광중합기 (Elipar FreeLight 2)를 사용하였다. 차단막을 1겹, 2겹, 4겹, 8겹으로 광중합기의 광섬유말단을 감싸고 휴대용 광강도 측정기 (Cure Rite)로 광중합기의 광강도를 측정하였다. 광중합기를 주문제작한 optical breadboard에 고정시킨 후 휴대용 spectroradiometer (CS-1000)를 이용하여 광중합기의 파장을 측정하였고, DSLR (Nikon D70s)을 이용하여 광중합기의 light diffusion을 사진 촬영하였다. 결과: 광강도 측정 결과는 차단막의 두께가 증가할수록 광강도가 유의하게 감소하였으나 할로겐 광중합기에서 1겹과 2겹 사이에는 유의차가 없었으며, 4겹 이상의 차단막을 투과할 때 광강도가 더 많이 감소하였다. 여러 겹의 차단막을 투과한 광중합기의 전체적인 파장 형태와 peak wavelength의 변화는 관찰되지 않았다. Light diffusion 사진 촬영 시, LED 광중합기에서는 차단막의 두께가 미치는 영향이 없었으나 할로겐 광중합기에서는 차단막을 4겹 사용했을 때부터 중합광이 조사되는 각도가 감소하기 시작하여 8겹 사용했을 때 통계적으로 유의하게 감소하는 것을 볼 수 있었다 (p < 0.05). 결론: 광중합형 복합레진을 광중합할 경우 감염 조절용 차단막이 찢어지는 경우를 대비하여 1겹으로 사용하기 보다는 2겹으로 사용하는 것이 환자간의 교차감염을 예방하는데 유리할 것으로 사료된다. Objectives: This study investigated the effect of infection control barrier thickness on power density, wavelength, and light diffusion of light curing units. Materials and Methods: Infection control barrier (Cleanwrap) in one-fold, two-fold, four-fold, and eightfold, and a halogen light curing unit (Optilux 360) and a light emitting diode (LED) light curing unit (Elipar FreeLight 2) were used in this study. Power density of light curing units with infection control barriers covering the fiberoptic bundle was measured with a hand held dental radiometer (Cure Rite). Wavelength of light curing units fixed on a custom made optical breadboard was measured with a portable spectroradiometer (CS-1000). Light diffusion of light curing units was photographed with DSLR (Nikon D70s) as above. Results: Power density decreased significantly as the layer thickness of the infection control barrier increased, except the one-fold and two-fold in halogen light curing unit. Especially, when the barrier was four-fold and more in the halogen light curing unit, the decrease of power density was more prominent. The wavelength of light curing units was not affected by the barriers and almost no change was detected in the peak wavelength. Light diffusion of LED light curing unit was not affected by barriers, however, halogen light curing unit showed decrease in light diffusion angle when the barrier was four-fold and statistically different decrease when the barrier was eight-fold (p < 0.05). Conclusions: It could be assumed that the infection control barriers should be used as two-fold rather than one-fold to prevent tearing of the barriers and subsequent cross contamination between the patients.

[PG-0007] Identification and Determination of Phenolic compounds in Rapeseed (Brassica napus L.) Mutant Lines Derived from Gamma-ray

Jaihyunk Ryu(Jaihyunk Ryu ),Baul Yang(Baul Yang ),Ye-Jin Lee(Ye-Jin Lee ),Dong-Gun Kim(Dong-Gun Kim ),Sang Hoon Kim(Sang Hoon Kim ),Sang-Il Han(Sang-Il Han ),Bo-Keun Ha(Bo-Keun Ha ),Si-Yong Kang(Si-Yo 한국육종학회 2022 한국육종학회 공동학술발표집 Vol.2022 No.-

Anti-stress Effect of Scutellaria baicalensis in SD Rats and ICR Mice

( Jong Hoon Ryu ),( Blendyl Saguan Tan Lee ),( Ji Wook Jung ),( Nam Yoon Ahn ),( Seung Joo Lee ),( Gu Young Yu ),( Shin Ha Han ),( Jeong Hoon Lee ),( Geum Seon Lee ),( Jae Hoon Cheong ) 한국응용약물학회 2004 Biomolecules & Therapeutics(구 응용약물학회지) Vol.12 No.1

N/A Pyroligneous liquid(PL) is produced by carbonizing Oak in 350∼400℃. It is traditionally used for treating stress-related disorder, hepatic disease, immune disorder, G-I disorder and inflammatory disease. The aim of this study is to investigate anti-stress effects of PL. The experiments were performed with the use of young(9 weeks of age) male rats of SD strain and the male ICR mice (20-25g). Animals of the normal group were not exposed to any stress and the control group were exposed to stress. The rats of the Ginseng, diazepam(BZ) and PL supplementary group were orally administered once a day 100g of Ginseng extract-kg body weight, 5 mg of BZ/kg body weight and 1 ml of PL/100 g body weight and then exposed to stress. The mice of the Ginseng, BZ and PL supplementary group were given water containing 100 g of Ginseng extract/100 ml potable water, 5 mg of BZ/kg 100 ml of drinking water and 10 ml of PL/100 ml of drinking water and exposed to stress. Animals were given materials for 7 days after stabilizing them, and then were given supplementary materials for 5 days with stress. They were stressed by immobilization for 30 minutes and then the animals were exposed to electroshocks for 5 minutes. We recorded stress-related behavioral changes of experimental animals by stressing them using the Etho-vision system and measured the levels of corticosterone in blood While stress suppressed locomotor activity of animals, PL-supplementation partially blocked the stress effect of locomotion in rats and mice, and also partially blocked stress-induced behavioral changes such as freezing, burrowing, smelling and rearing activity in rats and in closed area it increased relatively in elevated plus maze test. However, these changes also partially were blocked by PL-supplementation. PL-supplementation decreased levels of blood corticosterone increased by stress in rats. These results suggest that PL protects partially the living organism from stress attack in some cases.

A Patient with Pyridoxine-Dependent Epilepsy Who Was Treated with Triple Therapy

Minsun Ryu(Minsun Ryu),Ji-Hoon Na(Ji-Hoon Na),Hyunjoo Lee(Hyunjoo Lee),Young-Mock Lee(Young-Mock Lee) 대한소아신경학회 2022 대한소아신경학회지 Vol.30 No.4

[Poster Presentation] NEUTRAL DEINKING OF OLD NEWSPRINTS CONTAMINATED WITH FLEXO INK

Hoon Ryu,Hak Lae Lee 한국펄프·종이공학회 1999 한국펄프종이학회 기타 간행물 Vol.- No.-

Presence of small amount of old newspapers printed with waterbased flexographic inks leads to a significant loss of brightness of the deinked pulp by flotation process. Recently there has been an increased interests in employing neutral deinking processes to solve the problems associated with waterbased flexo ink.<br/> A comparative experimental study was performed to evaluate the efficiency of neutral de inking and conventional alkaline de inking for ONP furnishes contaminated with flexo ink. Effects of the de inking processes on pulp quality and white water characteristics were examined. It was shown that neutral deinking would provide significant improvement in brightness, yield, and freeness as compared with conventional alkaline deinking. There was no increase change in brightness when OMG was added in the furnish up to 20%, and it was attributed to the redeposition of flexo inks on chemical pulp fibers.

Absolute Configuration of ${\beta}$-agarofuran nucleus of euojaponine C by CD exciton chirality method

Ryu, Jae-Ha,Ryu, Shi-Yong,Han, Yong-Nam,Han, Byung-Hoon The Pharmaceutical Society of Korea 1997 Archives of Pharmacal Research Vol.20 No.1

A new celastraceae alkaloid, euojaponine C has been isolated from the methanol extract of the root bark of Euonymus japonica. With the relative stereochemistry of euojaponine C established by NOESY data, the absolute stereochemistry has been determined by circular dichroism (CD) exciton chirality method. The CD of the 2, 5-bis-phenyl benzoate of triacetonide derived from the LiAlH$_{4}$, hydrolysate, euonyminol shows that the configuration of C-2 and C-5 are both R.

Successful one-step endoscopic treatment of esophageal web of plummer-vinson syndrome and concomitant gastric carcinoid

Hoon Jai Chun,Kwang Gyun Lee,Ju Hyung Kim,Jong Jin Hyun,Eun Sun Kim,Sung Chul Park,Sang Hoon Park,Bo Ra Keum,Yeon Seok Seo,Yoon Tae Jeen,Soon Ho Um,Chang Duck Kim,Ho Sang Ryu 대한내과학회 2009 대한내과학회 추계학술대회 Vol.2009 No.-

A Hexane Fraction of Guava Leaves (Psidium guajava L.) Induces Anticancer Activity by Suppressing AKT/Mammalian Target of Rapamycin/Ribosomal p70 S6 Kinase in Human Prostate Cancer Cells

Ryu, Nae-Hyung,Park, Kyung-Ran,Kim, Sung-Moo,Yun, Hyung-Mun,Nam, Dong-Woo,Lee, Seok-Geun,Jang, Hyeung-Jin,Ahn, Kyoo-Seok,Kim, Sung-Hoon,Shim, Bum-Sang,Choi, Seung-Hoon,Mosaddik, Ashik,Cho, So-Mi K.,Ah The Korean Society of Food Science and Nutrition 2012 Journal of medicinal food Vol.15 No.3

This study was carried out to evaluate the anticancer effect of guava leaf extracts and its fractions. The chemical compositions of the active extracts were also determined. In the present study, we set out to determine whether the anticancer effects of guava leaves are linked with their ability to suppress constitutive AKT/mammalian target of rapamycin (mTOR)/ribosomal p70 S6 kinase (S6K1) and mitogen-activated protein kinase (MAPK) activation pathways in human prostate cancer cells. We found that guava leaf hexane fraction (GHF) was the most potent inducer of cytotoxic and apoptotic effects in PC-3 cells. The molecular mechanism or mechanisms of GHF apoptotic potential were correlated with the suppression of AKT/mTOR/S6K1 and MAPK signaling pathways. This effect of GHF correlated with down-regulation of various proteins that mediate cell proliferation, cell survival, metastasis, and angiogenesis. Analysis of GHF by gas chromatography and gas chromatography-mass spectrometry tentatively identified 60 compounds, including <TEX>${\beta}$</TEX>-eudesmol (11.98%), <TEX>${\alpha}$</TEX>-copaene (7.97%), phytol (7.95%), <TEX>${\alpha}$</TEX>-patchoulene (3.76%), <TEX>${\beta}$</TEX>-caryophyllene oxide (CPO) (3.63%), caryophylla-3(15),7(14)-dien-6-ol (2.68%), (E)-methyl isoeugenol (1.90%), <TEX>${\alpha}$</TEX>-terpineol (1.76%), and octadecane (1.23%). Besides GHF, CPO, but not phytol, also inhibited the AKT/mTOR/S6K1 signaling pathway and induced apoptosis in prostate cancer cells. Overall, these findings suggest that guava leaves can interfere with multiple signaling cascades linked with tumorigenesis and provide a source of potential therapeutic compounds for both the prevention and treatment of cancer.

Modulation of Neuroendocrinological Function by Psychosomatic Training : I.Acute Effect of Qi-Training on Growth Hormone,Insuline-Like Growth Factor(Igf)-I, and Insulin-Like Growth Factor Binding Protein (Igfbp)-3 in Men I.기수련이 성장호르몬, 인슐린양 성장인자-1 및 성장인자 결합단백질-3에 미치는 영향

Ryu, Hoon,Lee, Myeong-Soo,Park, Jae-Whang,Chung, Hun-Taeg 圓光大學校 醫科學硏究所 1998 圓光醫科學 Vol.14 No.2

신경내분비계는 심리적상태와 면역계를 연결시켜주는 중요한 고리로 작용하고 있다. 본 논문은 전통적심신수련인 기수련이 성장호르몬, 인슐린양성장인자-Ⅰ과 인슐린양성장인자의 결합단백질-3에 대한 효과를 살펴보았다. 수련전, 중, 후의 이들의 변화양상을 조사하기 위하여 방사면역측정법과 western ligand blot (WLB)분석을 사용하였다. 성장호르몬은 수련전에 비해 수련중에 유의하게 증가하였으며 (P<0.05), 인슐린양 성장인자-Ⅰ도 수련중 (P<0.01)과 수련후 (P<0.01)에 유의하게 증가하였다. 또한 성장호르몬과 인슐린양 성장인자-Ⅰ 사이에는 수련후에 유의한 상호관계가 있었다 (r=0.69, p<0.01). 인슐린양성장인자의 결합단백질-3은 수련중에 43-kDa 부분에서 상대적인 변화가 존재하였다(P<0.01). 이러한 결과는 기수련이 뇌-표적 기관 축의 변화를 통하여 성장호르몬, 인슐린양성장인자-Ⅰ과 인슐린양성장인자의 결합단백질-3에 변화를 주는 것으로 사료된다. The neuroendocrine system is regarded as the major link between the psychological state and the immune system in man. The present study was undertaken to examine the acute effect of Qi-training, a traditional psychosomatic training, on the plasma level of growth hormone (GH), insulin-like growth factor (IGF)-Ⅰ and insulin like growth factor binding protein (IGFBP)-3 in young men. To characterize the plasma level of hormones and the pattern of IGFBP-3 at pre- (-10 min), mid-time (40 min), and immediately after (post-time, +10 min) Qi-training, radioimmunoassay (RIA) and western ligand blot (WLB) analysis were used. The plasma level of GH at the mid-time was significantly increased (P<0.05) compared to the level at pre-time of Qi-training. The plasma level of IGF-Ⅰ was also increased at mid-time (P<0.01) and immediately after Qi-training. There was a significant correlation between the levels of GH and IGF-Ⅰ immediately after Qi-training (r=0.69, P<0.01). In this study the change of IGFBP-3 among the several IGFBPs was determined. There was a relative shift in IGFBP-3 for the 43-kDa fraction during mid-time of Qi-training (P<0.01). These results suggest that Qi-training influences the growth hormone, IGF-Ⅰ and IGFBP-3 status via brain-target organ axis in man.