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Xu, Hongtao,Wu, Changjin,Xiahou, Zhao,Jung, Ranju,Li, Ying,Liu, Chunli Springer US 2017 Nanoscale research letters Vol.12 No.1
<P>Five percent of Fe-doped ZnO (ZnO:Fe) thin films were deposited on Pt/TiO<SUB>2</SUB>/SiO<SUB>2</SUB>/Si substrates by a spin-coating method. The films were annealed without (ZnO:Fe-0T) and with a pulsed magnetic field of 4 T (ZnO:Fe-4TP) to investigate the magnetic annealing effect on the resistance switching (RS) behavior of the Pt/ZnO:Fe/Pt structures. Compared with the ZnO:Fe-0T film, the ZnO:Fe-4TP film showed improved RS performance regarding the stability of the set voltage and the resistance of the high resistance state. Transmission electron microscopy and X-ray photoelectron spectroscopy analyses revealed that the ZnO:Fe-4TP film contains more uniform grains and a higher density of oxygen vacancies, which promote the easier formation of conducting filaments along similar paths and the stability of switching parameters. These results suggest that external magnetic fields can be used to prepare magnetic oxide thin films with improved resistance switching performance for memory device applications.</P>
Molecular discrimination of Panax ginseng cultivar K-1 using pathogenesis-related protein 5 gene
Hongtao Wang,Fengjiao Xu,Xinqi Wang,권우생,Deok Chun Yang 고려인삼학회 2019 Journal of Ginseng Research Vol.43 No.3
Background: The mixed-cultivation of different Panax ginseng cultivars can cause adverse effects onstability of yield and quality. K-1 is a superior cultivar with good root shape and stronger diseaseresistance. DNA markers mined from functional genes are clearly desirable for K-1, as they may associatewith major traits and can be used for marker-assisted selection to maintain the high quality of Koreanginseng. Methods: Five genes encoding pathogenesis-related (PR) proteins of P. ginseng were amplified andcompared for polymorphism mining. Primary, secondary, and tertiary structures of PR5 protein wereanalyzed by ExPASy-ProtParam, PSSpred, and I-TASSER methods, respectively. A coding single nucleotidepolymorphism (SNP)ebased specific primer was designed for K-1 by introducing a destabilizingmismatch within the 30 end. Allele-specific polymerase chain reaction (PCR) and real-time allele-specificPCR assays were conducted for molecular discrimination of K-1 from other cultivars and landraces. Results: A coding SNP leading to the modification of amino acid residue from aspartic acid to asparaginewas exploited in PR5 gene of K-1 cultivar. Bioinformatics analysis showed that the modification of aminoacid residue changed the secondary and tertiary structures of the PR5 protein. Primer KSR was designedfor specific discrimination of K-1 from other ginseng cultivars and landraces. The developed real-timeallele-specific PCR assay enabled easier automation and accurate genotyping of K-1 from a large numberof ginseng samples. Conclusion: The SNP marker and the developed real-time allele-specific PCR assay will be useful not onlyfor marker-assisted selection of K-1 cultivar but also for quality control in breeding and seed programs ofP. ginseng.
Hongtao Shen,Weicheng Hu,Qing-shan Yang,Fucheng Yang,Kunpeng Guo,Tong Zhou,Guowei Qian,Qinggen Xu,Ziting Yuan 한국풍공학회 2022 Wind and Structures, An International Journal (WAS Vol.35 No.6
In wind-resistant designs, wind velocity is assumed to be a Gaussian process; however, local complex topography may result in strong non-Gaussian wind features. This study investigates the non-Gaussian wind features over complex terrain under atmospheric turbulent boundary layers by the large eddy simulation (LES) model, and the turbulent inlet of LES is generated by the consistent discretizing random flow generation (CDRFG) method. The performance of LES is validated by two different complex terrains in Changsha and Mianyang, China, and the results are compared with wind tunnel tests and onsite measurements, respectively. Furthermore, the non-Gaussian parameters, such as skewness, kurtosis, probability curves, and gust factors, are analyzed in-depth. The results show that the LES method is in good agreement with both mean and turbulent wind fields from wind tunnel tests and onsite measurements. Wind fields in complex terrain mostly exhibit a left-skewed Gaussian process, and it changes from a softening Gaussian process to a hardening Gaussian process as the height increases. A reduction in the gust factors of about 2.0%-15.0% can be found by taking into account the non-Gaussian features, except for a 4.4% increase near the ground in steep terrain. This study can provide a reference for the assessment of extreme wind loads on structures in complex terrain.
Xu Tao,Wang Chutong,Li Minying,Wei Jing,He Zixuan,Qian Zhongqing,Wang Xiaojing,Wang Hongtao 한국미생물학회 2024 The journal of microbiology Vol.62 No.1
Tuberculosis (TB), a bacterial infectious disease caused by Mycobacterium tuberculosis (M. tuberculosis), is a significant global public health problem. Mycobacterium tuberculosis expresses a unique family of PE_PGRS proteins that have been implicated in pathogenesis. Despite numerous studies, the functions of most PE_PGRS proteins in the pathogenesis of mycobacterium infections remain unclear. PE_PGRS45 (Rv2615c) is only found in pathogenic mycobacteria. In this study, we successfully constructed a recombinant Mycobacterium smegmatis (M. smegmatis) strain which heterologously expresses the PE_PGRS45 protein. We found that overexpression of this cell wall-associated protein enhanced bacterial viability under stress in vitro and cell survival in macrophages. MS_PE_PGRS45 decreased the secretion of pro-inflammatory cytokines such as IL-1β, IL-6, IL-12p40, and TNF-α. We also found that MS_PE_PGRS45 increased the expression of the anti-inflammatory cytokine IL-10 and altered macrophage-mediated immune responses. Furthermore, PE_PGRS45 enhanced the survival rate of M. smegmatis in macrophages by inhibiting cell apoptosis. Collectively, our findings show that PE_PGRS45 is a virulent factor actively involved in the interaction with the host macrophage.
Molecular discrimination of Panax ginseng cultivar K-1 using pathogenesis-related protein 5 gene
Wang, Hongtao,Xu, Fengjiao,Wang, Xinqi,Kwon, Woo-Saeng,Yang, Deok-Chun The Korean Society of Ginseng 2019 Journal of Ginseng Research Vol.43 No.3
Background: The mixed-cultivation of different Panax ginseng cultivars can cause adverse effects on stability of yield and quality. K-1 is a superior cultivar with good root shape and stronger disease resistance. DNA markers mined from functional genes are clearly desirable for K-1, as they may associate with major traits and can be used for marker-assisted selection to maintain the high quality of Korean ginseng. Methods: Five genes encoding pathogenesis-related (PR) proteins of P. ginseng were amplified and compared for polymorphism mining. Primary, secondary, and tertiary structures of PR5 protein were analyzed by ExPASy-ProtParam, PSSpred, and I-TASSER methods, respectively. A coding single nucleotide polymorphism (SNP)-based specific primer was designed for K-1 by introducing a destabilizing mismatch within the 3' end. Allele-specific polymerase chain reaction (PCR) and real-time allele-specific PCR assays were conducted for molecular discrimination of K-1 from other cultivars and landraces. Results: A coding SNP leading to the modification of amino acid residue from aspartic acid to asparagine was exploited in PR5 gene of K-1 cultivar. Bioinformatics analysis showed that the modification of amino acid residue changed the secondary and tertiary structures of the PR5 protein. Primer KSR was designed for specific discrimination of K-1 from other ginseng cultivars and landraces. The developed real-time allele-specific PCR assay enabled easier automation and accurate genotyping of K-1 from a large number of ginseng samples. Conclusion: The SNP marker and the developed real-time allele-specific PCR assay will be useful not only for marker-assisted selection of K-1 cultivar but also for quality control in breeding and seed programs of P. ginseng.
Fengyu Xu,Xingsong Wang,Hongtao Wu 대한기계학회 2012 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.26 No.3
A nondestructive testing technique based on magnetic flux leakage is presented to inspect automatically the stay cables with large diameters of a cable-stayed bridge. Using the proposed inspection method, an online nondestructive testing (NDT) modular sensor is developed. The wreath-like sensor is composed of several sensor units that embrace the cable at equal angles. Each sensor unit consists of two permanent magnets and a hall sensor to detect the magnetic flux density. The modular sensor can be installed conveniently on cables with various diameters by increasing the number of sensor units and adjusting the relative distances between adjacent sensor units. Results of the experiments performed on a man-made cable with faults prove that the proposed sensor can inspect the status signals of the inner wires of the cables. To filter the interfering signals, three processing algorithms are discussed, including the moving average method,improved detrending algorithm, and signal processing based on a digital filter. Results show that the developed NDT sensor carried by a cable inspection robot can move along the cable and monitor the state of the stay cables.
( Tao Xu ),( Minying Li ),( Chutong Wang ),( Meili Yuan ),( Xianyou Chang ),( Zhongqing Qian ),( Baiqing Li ),( Meiqun Sun ),( Hongtao Wang ) 한국미생물 · 생명공학회 2021 Journal of microbiology and biotechnology Vol.31 No.11
Studies have demonstrated that PE_PGRS45 is constitutively expressed under various environmental conditions (such as nutrient depletion, hypoxia, and low pH) of the in vitro growth conditions examined, indicating that PE_PGRS45 protein is critical to the basic functions of Mycobacterium tuberculosis. However, there are few reports about the biochemical function and pathogenic mechanism of PE_PGRS45 protein. The fact that this M. tuberculosis gene is not easily expressed in E. coli may be mainly due to the high content of G+C and the use of unique codons. Fusion tags are indispensable tools used to improve the soluble expression of recombinant proteins and accelerate the characterization of protein structure and function. In the present study, His6, Trx, and His6-MBP were used as fusion tags, but only MBP-PE_PGRS45 was expressed solubly. The purification using His6-MBP tag-specific binding to the Ni column was easy to separate after the tag cleavage. We used the purified PE_PGRS45 to immunize New Zealand rabbits and obtained anti- PE_PGRS45 serum. We found that the titer of polyclonal antibodies against PE_PGR45 was higher than 1:256000. The result shows that purified PE_PGRS45 can induce New Zealand rabbits to produce high-titer antibodies. In conclusion, the recombinant protein PE_PGRS45 was successfully expressed in E. coli and specific antiserum was prepared, which will be followed by further evaluation of these specific antigens to develop highly sensitive and specific diagnostic tests for tuberculosis.