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RISS 인기검색어
- 검색키워드
- 저자 : Hironobu Sasano (검색결과 2 건)
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Moon, Ju-Yeon,McNamara, Keely May,Lee, Jung-Jin,Chung, Bong Chul,Sasano, Hironobu,Choi, Man Ho Elsevier 2018 The Journal of steroid biochemistry and molecular Vol.178 No.-
<P><B>Abstract</B></P> <P>Sex steroids in clinical endocrinology have been mainly investigated with peripheral blood and urine samples, while there is limited information regarding the local levels within tissues. To improve analytical properties of sex steroids from trace amounts of tissue samples, two-phase extractive ethoxycarbonlyation and subsequent pentafluoropropionyl derivatization coupled to gas chromatography–tandem mass spectrometry (GC–MS/MS) was developed. The optimized analytical conditions led to excellent chromatographic separation of 15 estrogens, 6 androgens, and 2 progestins. The quantitative results were calculated based on <I>in-house</I> control samples as the steroid-free tissues, and the precision and accuracy were 4.2%–26.8% and 90.8%–116.4%, respectively. The on-column limit of quantification was from 180 fg to 0.5 pg for androgens and estrogens, and 1.25 pg for progestins, which were found to be linear (<I>r</I> <SUP>2 </SUP>> 0.990). The validated method was then applied to quantify 7 sex steroids from three 100-μm-thick frozen breast tissue slices from postmenopausal patients with breast cancer. This is the first report on the improved GC–MS/MS method for the detection of androgens and pregnenolone from breast cancer tissues, and it can be a useful technique to measure the local levels of sex steroids, thus, enhancing our understanding of the pathophysiological significances of steroidogenesis.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Sex steroids were measured from trace amounts of tissue samples. </LI> <LI> Extractable alkyloxycarbonylation improved chromatographic properties. </LI> <LI> Pentafluroropropionylation produces characteristic ions in mass spectrometry. </LI> <LI> GC–MS/MS resulted in a sensitive detection of sex steroids from frozen breast tissue slices. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
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Xin Gao,Yuto Yamazaki,Yuta Tezuka,Kei Omata,Yoshikiyo Ono,Ryo Morimoto,Yasuhiro Nakamura,Fumitoshi Satoh,Hironobu Sasano 대한내분비학회 2021 Endocrinology and metabolism Vol.36 No.1
Primary aldosteronism (PA) is the most common cause of secondary hypertension, and is associated with an increased incidence of cardiovascular events. PA itself is clinically classified into the following two types: unilateral PA, mostly composed of aldosteroneproducing adenoma (APA); and bilateral hyperaldosteronism, consisting of multiple aldosterone-producing micronodules (APMs) and aldosterone-producing diffuse hyperplasia. Histopathologically, those disorders above are all composed of compact and clear cells. The cellular morphology in the above-mentioned aldosterone-producing disorders has been recently reported to be closely correlated with patterns of somatic mutations of ion channels including KCNJ5, CACNA1D, ATP1A1, ATP2B3, and others. In addition,in non-pathological adrenal glands, APMs are frequently detected regardless of the status of the renin-angiotensin-aldosterone system (RAAS). Aldosterone-producing nodules have been also proposed as non-neoplastic nodules that can be identified by hematoxylin and eosin staining. These non-neoplastic CYP11B2-positive nodules could represent possible precursors of APAs possibly due to the presence of somatic mutations. On the other hand, aging itself also plays a pivotal role in the development of aldosterone-producing lesions. For instance, the number of APMs was also reported to increase with aging. Therefore, recent studies indicated the novel classification of PA into normotensive PA (RAAS-independent APM) and clinically overt PA.
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