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Heo, June Seok,Kim, Hyun Ok,Song, Seung Yong,Lew, Dae Hyun,Choi, Youjeong,Kim, Sinyoung Hindawi Publishing Corporation 2016 BioMed research international Vol.2016 No.-
<P>Mesenchymal stem cells (MSCs) possess great therapeutic potential. Efficient<I> in vitro</I> expansion of MSCs is however necessary for their clinical application. The extracellular matrix (ECM) provides structural and biochemical support to the surrounding cells, and it has been used as a coating substrate for cell culture. In this study, we have aimed to improve the functionality and stemness of MSCs during culture using poly-L-lysine (PLL). Functionality of MSCs was analysed by cell cycle analysis, differentiation assay, β-galactosidase staining, and RT-PCR. Furthermore, we assessed the global gene expression profile of MSCs on uncoated and PLL-coated plates. MSCs on PLL-coated plates exhibited a faster growth rate with increased S-phase and upregulated expression of the stemness markers. In addition, their osteogenic differentiation potential was increased, and genes involved in cell adhesion, FGF-2 signalling, cell cycle, stemness, cell differentiation, and cell proliferation were upregulated, compared to that of the MSCs cultured on uncoated plates. We also confirmed that MSCs on uncoated plates expressed higher β-galactosidase than the MSCs on PLL-coated plates. We demonstrate that PLL provides favourable microenvironment for MSC culture by reversing the replicative senescence. This method will significantly contribute to effective preparation of MSCs for cellular therapy.</P>
Seok Kim,Jaehoon Jung,Hwajin Kim,Rok Won Heo,Chin-ok Yi,Jung Eun Lee,Byeong Tak Jeon,Won-Ho Kim,Jong Ryeal Hahm,Gu Seob Roh 대한생리학회-대한약리학회 2014 The Korean Journal of Physiology & Pharmacology Vol.18 No.4
Exendin-4 (Ex-4), a glucagon-like peptide-1 receptor (GLP-1R) agonist, has been known to reverse hepatic steatosis in ob/ob mice. Although many studies have evaluated molecular targets of Ex-4, its mechanism of action on hepatic steatosis and fibrosis has not fully been determined. In the liver, glucose transporter 4 (GLUT4) is mainly expressed in hepatocytes, endothelial cells and hepatic stellate cells (HSCs). In the present study, the effects of Ex-4 on GLUT4 expression were determined in the liver of ob/ob mice. Ob/ob mice were treated with Ex-4 for 10 weeks. Serum metabolic parameters, hepatic triglyceride levels, and liver tissues were evaluated for hepatic steatosis. The weights of the whole body and liver in ob/ob mice were reduced by long-term Ex-4 treatment. Serum metabolic parameters, hepatic steatosis, and hepatic fibrosis in ob/ob mice were reduced by Ex-4. Particularly, Ex-4 improved hepatic steatosis by enhancing GLUT4 via GLP-1R activation in ob/ob mice. Ex-4 treatment also inhibited hepatic fibrosis by decreasing expression of connective tissue growth factor in HSCs of ob/ob mice. Our data suggest that GLP-1 agonists exert a protective effect on hepatic steatosis and fibrosis in obesity and type 2 diabetes.
HEO, JUNE SEOK,CHOI, YOUJEONG,KIM, HAN-SOO,KIM, HYUN OK UNKNOWN 2016 INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE Vol.37 No.1
<P>Mesenchymal stem cells (MSCs) are clinically useful due to their capacity for self-renewal, their immunomodulatory properties and tissue regenerative potential. These cells can be isolated from various tissues and exhibit different potential for clinical applications according to their origin, and thus comparative studies on MSCs from different tissues are essential. In this study, we investigated the immunophenotype, proliferative potential, multilineage differentiation and immunomodulatory capacity of MSCs derived from different tissue sources, namely bone marrow, adipose tissue, the placenta and umbilical cord blood. The gene expression profiles of stemness-related genes [octamer-binding transcription factor 4 (<I>OCT4</I>), sex determining region Y-box (<I>SOX)2</I>, <I>MYC</I>, Krüppel-like factor 4 (<I>KLF4</I>), <I>NANOG</I>, <I>LIN28</I> and <I>REX1</I>] and lineage-related and differentiation stage-related genes [<I>B4GALNT1 (GM2/GS2 synthase)</I>, inhibin, beta A (<I>INHBA</I>), distal-less homeobox 5 (<I>DLX5</I>), runt-related transcription factor 2 (<I>RUNX2</I>), proliferator-activated receptor gamma (<I>PPARG</I>), CCAAT/enhancer-binding protein alpha (<I>C/EBPA</I>), bone morphogenetic protein 7 (<I>BMP7</I>) and <I>SOX9</I>] were compared using RT-PCR. No significant differences in growth rate, colony-forming efficiency and immunophenotype were observed. Our results demonstrated that MSCs derived from bone marrow and adipose tissue shared not only <I>in vitro</I> trilineage differentiation potential, but also gene expression profiles. While there was considerable interdonor variation in <I>DLX5</I> expression between MSCs derived from different tissues, its expression appears to be associated with the osteogenic potential of MSCs. Bone marrow-derived MSCs (BM-MSCs) significantly inhibited allogeneic T cell proliferation possibly via the high levels of the immunosuppressive cytokines, <I>IL10</I> and <I>TGFB1</I>. Although MSCs derived from different tissues and fibroblasts share many characteristics, some of the marker genes, such as <I>B4GALNT1</I> and <I>DLX5</I> may be useful for the characterization of MSCs derived from different tissue sources. Collectively, our results suggest that, based on their tri-lineage differentiation potential and immunomodulatory effects, BM-MSCs and adipose tissue-derived MSCs (A-MSCs) represent the optimal stem cell source for tissue engineering and regenerative medicine.</P>
First Detection of bla(IMP-1) in Clinical Isolate Multiresistant Acinetobacter baumannii from Korea
( Seok Hoon Jeong ),( Il Kwon Bae ),( Seung Ghyu Sohn ),( Kwang Ok Park ),( Young Jun An ),( Kwang Hoon Sung ),( Seon Ju Jang ),( Myong Jin Heo ),( Ki Suk Yang ),( Sang Hee Lee ) 한국미생물생명공학회 2006 Journal of microbiology and biotechnology Vol.16 No.9
Application of Botulinum Toxin on Masticatory Muscle of Patients with Bruxism
Jang, Seok-Min,Jeon, Hye-Mi,Kim, Kyung-Hee,Ok, Soo-Min,Heo, Jun-Young,Jeong, Sung-Hee,Ahn, Yong-Woo Korean Academy of Orofacial Pain and Oral Medicine 2014 Journal of Oral Medicine and Pain Vol.39 No.2
Purpose: This study aims to evaluate the changes in soft tissue thickness of the masseteric region after injection of botulinum toxin type A (BTX-A). Methods: Twenty-four data acquired from medical records were classified into 4 groups: bruxer group that received masseter muscle injection only (M-B), bruxer group that received both masseter and temporalis muscle injections (MT-B), non-bruxer group that received masseter muscle injection only (M-NB) and non-bruxer group that received both masseter and temporalis muscle injections (MT-NB). Injection dose of BTX-A was 30 units for each masseter muscle and 20 units for each temporalis muscle. We measured the reduced thickness of the masseteric region before and after 12 weeks after injection using cone-beam computed tomography. Results: Among the patients that received both masseter and temporalis muscle injections, bruxer group showed a tendency to have more reduction in masseter muscle thickness than non-bruxer group. The difference in reduced thickness between M-B and MT-B tended to show greater than the difference between M-NB and MT-NB. Conclusions: In case of masseter hypertrothy patients with bruxism there was a tendency to show a difference in reduced thickness of soft tissue between the group that received both masseter and temporalis muscles injection and the group that received masseter muscle injection only hence a thorough inspection before the injection of BTX-A is condisered to be needed.
Kim, Seok,Jung, Jaehoon,Kim, Hwajin,Heo, Rok Won,Yi, Chin-Ok,Lee, Jung Eun,Jeon, Byeong Tak,Kim, Won-Ho,Hahm, Jong Ryeal,Roh, Gu Seob The Korean Society of Pharmacology 2014 The Korean Journal of Physiology & Pharmacology Vol.18 No.4
Exendin-4 (Ex-4), a glucagon-like peptide-1 receptor (GLP-1R) agonist, has been known to reverse hepatic steatosis in ob/ob mice. Although many studies have evaluated molecular targets of Ex-4, its mechanism of action on hepatic steatosis and fibrosis has not fully been determined. In the liver, glucose transporter 4 (GLUT4) is mainly expressed in hepatocytes, endothelial cells and hepatic stellate cells (HSCs). In the present study, the effects of Ex-4 on GLUT4 expression were determined in the liver of ob/ob mice. Ob/ob mice were treated with Ex-4 for 10 weeks. Serum metabolic parameters, hepatic triglyceride levels, and liver tissues were evaluated for hepatic steatosis. The weights of the whole body and liver in ob/ob mice were reduced by long-term Ex-4 treatment. Serum metabolic parameters, hepatic steatosis, and hepatic fibrosis in ob/ob mice were reduced by Ex-4. Particularly, Ex-4 improved hepatic steatosis by enhancing GLUT4 via GLP-1R activation in ob/ob mice. Ex-4 treatment also inhibited hepatic fibrosis by decreasing expression of connective tissue growth factor in HSCs of ob/ob mice. Our data suggest that GLP-1 agonists exert a protective effect on hepatic steatosis and fibrosis in obesity and type 2 diabetes.