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銅鑛山廢水로부터 分離한 구리 耐性菌(P.stutzeri)의 菌體內 구리 蓄積 特性
許鍾秀,曺周植,韓文圭,李洪宰,林永成 경상대학교 환경보전연구소 1994 環境保全硏究所報 Vol.2 No.1
Of the copper-tolerant 215 bacterial strains isolated from polluted river water, wastewater and activated sludges of wastewater treatment plants, or wastewater and soils of the copper mine aera in Gyeongnam province a strain, which showed considerably higher growth rate in the agar plate containing 2000ppm of copper than any other strains, was identified as a Pseudomonas stutzeri or its similar strain when analyzed by taxonomical charicteristics. Optimum pH and temperature for the growth of the P. stutzeri were 7.0 and 30℃ respectively. This strain was resistant to antibiotics, such as ampicillin and tetracycline, and heavy metals, such as lead and chromium. This strain utilized phenol benzen or toluene as a sole carbon source. The rate of copper accumulation in P. stutzeri cell was enhanced at low concentration of Cu in the growth media. The maximum copper absorption by this strain grown in 10ppm of Cu at 3 days after culture was 49%, but in 50ppm and 100ppm of Cu was 38% and 32%, respectively. The optimal temprature and pH for the effective intracellular accumulation of copper were 30℃ and initial pH 8.0 under shaking culture.
구리 耐性菌(Pseudomonas stutzeri)의 菌體內 구리 蓄積機作
許鍾秀,曺周植,韓文圭,李洪宰,林永成 경상대학교 환경보전연구소 1994 環境保全硏究所報 Vol.2 No.1
In order to study the mechanism of the copper accumulation in the cell of copper-tolerant bacterium, Pseudomonas stutzeri, distribution of the copper in the cell were investigated. Approximately 61% of the accumulated copper was distributed on the cell wall and the other 39% portion was in cytoplasm. 78% of the copper in the cell wall fractions was present in the polyphosphate and polysaccharide fractions, but most of copper in the cytoplasm fractions was in protein and nucleic acid. Copper affected the protein synthesis in P. stutzeri. The intracellular protein content was decreased by copper addition, but the soluble protein precipitated by ammonium sulfate(30-75% saturation) was increased as compared to that from the cells grown without copper. Furthermore, in the cells grown with copper, high-molecular-weight soluble protein was increased, compared with the cells grown without copper, but low-molecular -weight soluble protein was decreased. These results indicate that copper inhibited the intracellular protein biosynthesis but enhanced the biosynthesis of the high-molecular-weight soluble protein precipitated by ammonium sulfate(30-75% saturation).
Pro-Inflammatory Role of S1P3 in Macrophages
( Jae-yeong Heo ),( Dong-soon Im ) 한국응용약물학회 2019 Biomolecules & Therapeutics(구 응용약물학회지) Vol.27 No.4
Sphingosine kinase 1 and its product, sphingosine 1-phosphate (S1P), as well as their receptors, have been implicated in inflammatory responses. The functions of receptors S1P<sub>1</sub> and S1P<sub>2</sub> on cell motility have been investigated. However, the function of S1P3 has been poorly investigated. In this study, the roles of S1P<sub>3</sub> on inflammatory response were investigated in primary peritoneal macrophages. S1P<sub>3</sub> receptor was induced along with sphingosine kinase 1 by stimulation of lipopolysaccharide (LPS). LPS treatment induced inflammatory genes, such iNOS, COX-2, IL-1β, IL-6 and TNF-α. TY52156, an antagonist of S1P<sub>3</sub> suppressed the induction of inflammatory genes in a concentration dependent manner. Suppression of iNOS and COX-2 induction was further confirmed by western blotting and NO measurement. Suppression of IL-1β induction was also confirmed by western blotting and ELISA. Caspase 1, which is responsible for IL-1β production, was similarly induced by LPS and suppressed by TY52156. Therefore, we have shown S1P<sub>3</sub> induction in the inflammatory conditions and its pro-inflammatory roles. Targeting S1P<sub>3</sub> might be a strategy for regulating inflammatory diseases.
Jae-Yeong Heo,RAJANGAMVINODH,Hee-JeKim,Rajendran Suresh Babu,Kungumaraj Krishna Kumar,Chandu V.V. Muralee Gopi,김성신 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.106 No.-
Herein, we synthesized 1D bimetallic hydrogen phosphate [CoxNix(HPO4)] nanorods by using a simpleand effective chemical bath deposition method for supercapacitor applications. The preparedCoxNix(HPO4) was analyzed by Fourier transform infrared (FT-IR) spectroscopy and X-ray diffraction(XRD) pattern. The surface morphology was envisaged by scanning electron microscopy (SEM) and transmissionelectron microscopy (TEM) methods. The porous nature and surface area of the materials werecharacterized by nitrogen sorption isotherm and a high specific surface area of 153 m2 g1 was foundto be for Co0.75Ni0.25(HPO4). The Co0.75Ni0.25(HPO4) displays a maximum specific capacity of475 mA h g1 at 1 A g1 in a three-electrode configuration using 3 M KOH as the electrolyte. Co0.75Ni0.25(HPO4) exhibits almost 94.8% of its initial specific capacity over 5000 GCD cycles at 10 Ag1. Furthermore, the fabricated asymmetric supercapacitor (ASC) with Co0.75Ni0.25(HPO4) and activatedcarbon (AC) showed a high specific capacitance of 182.5F g1 at 0.5 A g1. The ASC device delivered amaximum energy density of 64.88 Wh kg1 at a power density of 800 W kg1.
SrGa<sub>2</sub>S<sub>4</sub> 형광체의 합성과 발광 특성
허영덕(Heo, Yeong-Deok),심재훈(Sim, Jae-Hun),도영락(Do, Yeong-Rak) 대한화학회 2002 대한화학회지 Vol.46 No.2
:Eu는 녹색을 발광하는 형광체로 전계 방출 디스플레이, 음극선 발광 분야에 널리 응용되고 있다. 일반적으로 :Eu의 합성은 ,, 그리고 를 와 Ar 가스를 흘려주면서 고온에서 소송하는 고상반응법으로 합성하였다. 본 연구에서는 SrS, Eu 착물, 그리고 Ga 착물의 분해 반응을 통해서 :Eu 형광체를 합성하였다. 이 방법의 장점은 Ar 가스 뿐 만 아니라 독성의 를 사용하지 않는 것이다. :Eu 형광체의 합성 조건과 발광 특성을 검토하였다. SrGaS : Eu is a green emitting phosphor which is applied for field emission display, and cathodoluminescence. Conventionally, SrGaS : Eu is synthesized by solid state reaction, in which a mixture of SrCO, GaO, and EuO is fired at high temperatures under flowing HS and Ar gases. In this study,SrGaS : Eu phosphor is synthesized by using a decomposition method, where SrS, Eu complex, and Ga com-plex are used. The advantage of this method is that toxic HS gas and Ar gas are not used. The synthetic con-ditions and luminescent properties of SrGaS : Eu phosphor are also investigated.
A Morphometric Study of the Obturator Nerve around the Obturator Foramen
Jo, Se Yeong,Chang, Jae Chil,Bae, Hack Gun,Oh, Jae-Sang,Heo, Juneyoung,Hwang, Jae Chan The Korean Neurosurgical Society 2016 Journal of Korean neurosurgical society Vol.59 No.3
Objective : Obturator neuropathy is a rare condition. Many neurosurgeons are unfamiliar with the obturator nerve anatomy. The purpose of this study was to define obturator nerve landmarks around the obturator foramen. Methods : Fourteen cadavers were studied bilaterally to measure the distances from the nerve root to relevant anatomical landmarks near the obturator nerve, including the anterior superior iliac spine (ASIS), the pubic tubercle, the inguinal ligament, the femoral artery, and the adductor longus. Results : The obturator nerve exits the obturator foramen and travels infero-medially between the adductors longus and brevis. The median distances from the obturator nerve exit zone (ONEZ) to the ASIS and pubic tubercle were 114 mm and 30 mm, respectively. The median horizontal and vertical distances between the pubic tubercle and the ONEZ were 17 mm and 27 mm, respectively. The shortest median distance from the ONEZ to the inguinal ligament was 19 mm. The median inguinal ligament lengths from the ASIS and the median pubic tubercle to the shortest point were 103 mm and 24 mm, respectively. The median obturator nerve lengths between the ONEZ and the adductor longus and femoral artery were 41 mm and 28 mm, respectively. Conclusion : The obturator nerve exits the foramen 17 mm and 27 mm on the horizontal and sagittal planes, respectively, from the pubic tubercle below the pectineus muscle. The shallowest area is approximately one-fifth medially from the inguinal ligament. This study will help improve the accuracy of obturator nerve surgeries to better establish therapeutic plans and decrease complications.