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Hemasundar Alavilli,Kishor D.S,Kihwan Song 한국원예학회 2021 한국원예학회 학술발표요지 Vol.2021 No.10
The Cucumber is one of the widely cultivated and economically important vegetable crops across the world. Most of the cucumber cultivars have white flesh which is devoid of ß-carotene and contains lower amounts of total carotenoid contents compared to the orange or yellow-fleshed cucumbers which are augmented with ß-carotene. Considering the dietary and nutritional benefits of carotenoids, developing cucumber varieties with carotenoid enriched fruit flesh will serve as a nutrient-rich food source. In the line with findings previously, Lu et al. (2015) identified a genomic region in chromosome 7 that is responsible for yellow-fleshed fruit in cucumber. Further, to ascertain the responsible gene we performed high-throughput sequencing using CS-C (yellow-fleshed cucumber), along with JEF, and KWSA (white-fleshed cucumber) breeding lines using the HiSeq2000. The resultant reads were mapped to the ‘Chinese Long v3’ genome and found 7 DNA polymorphisms in 5 genes in specific to the CS-C breeding line within the previously reported genomic region. Among the 5 shortlisted genes, we found one of the gene with an SNP variation in the third exon resulted in an amino acid change in the CS-C breeding line. Further gene sequencing and segregation analysis also found to be following the Mendelian segregation ratio suggests, the hypothetical gene might be responsible for yellow endocarp phenotype in the CS-C line. In summary, our study identified a novel gene responsible for yellow endocarp cucumber and has a different genetic basis from the previously identified yellow fruit fleshed cucumbers. Our findings could provide a basis for future studies towards the breeding of carotenoid-rich cucumbers to counteract nutrient deficiency and malnutrition.
ALAVILLI HEMASUNDAR,Jeong‑Jin Lee,Chae‑Rin You,Sang‑Choon Lee,송기환 한국식물학회 2023 Journal of Plant Biology Vol.66 No.4
Chilling stress is one of the major abiotic constraints limiting cucurbit crop winter cultivation globally. Pumpkins (Cucurbita moschata) have been frequently used as a bloomless rootstock to enhance the fruit vigor and glossiness of cucumber (Cucumis sativus). Nonetheless, pumpkins are less tolerant to chilling stress than fig-leaf gourd (Cucurbita ficifolia), limiting their winter cultivation usage. Hence, it necessitates identifying chilling-tolerant pumpkin germplasm to identify the stress-responsive genes and to ease rootstock breeding in the face of unpredictable future climates. Our study considered the root fresh weight (RFW) and shoot fresh weight (SFW) growth under chilling stress as a tolerance index to assess the low-temperature tolerance to screen 407 pumpkin germplasm collection. Based on the preliminary characterization, we isolated the chilling-tolerant (IT 278592 [CT]) and the chilling-susceptible (IT 274039 [CS]) lines. The bulked-segregant analysis-assisted next-generation sequencing uncovered the existence of single nucleotide polymorphism (SNP) variations in three genes, including CmoCh06G003170 (encodes for F4P12_330), CmoCh16G010790 (Nucleoredoxin 1 [CmoNRX1]), and CmoCh15G013730 (DNA binding with one finger (Dof) protein 4.6 [CmoDof4.6]), while comparing the allelic variations between the CT and CS lines. Accordingly, Sanger sequencing and polymorphic marker analysis also asserted the presence of missense mutations in the three candidate genes. Despite SNP variation in three genes, the qRT-PCR analysis uncovered the chilling stress-responsive expression only in the CmoDof4.6 gene. The CmoDof4.6 gene was downregulated in the CT line compared to the CS line in response to the chilling stress implying that it could be a potential candidate gene and needed further experimental attention. Altogether, we identified a novel chilling stress-responsive gene in pumpkin germplasm that could facilitate the identification and breeding of chilling-tolerant pumpkin rootstock.
유채린,Hemasundar Alavilli,송기환 한국육종학회 2022 Plant Breeding and Biotechnology Vol.10 No.2
Pumpkin (Cucurbita moschata) has been increasingly used as bloomless rootstock for cucumber (Cucumis sativus), butit is sensitive to low-temperature, which is the major bottleneck for winter cultivation. Hence, to develop low-temperature tolerantrootstock varieties, it is needed to identify tolerant germplasm from a wide range of genetic resources. For this, we developed a selectioncriterion for a quick assessment of low-temperature tolerance in pumpkin germplasms from different geographical origins. Weconsidered various indexes for the fast evaluation of low-temperature tolerance, including seedling developmental stage, type ofseedling (excised or non-excised), growth space, etc. Under the testing condition (17℃/7℃, 8 hours light/16 hours dark, 150 μmol m-2s-1light intensity), we found a correlation (r=0.71*) in root growth between 3 weeks-cultured excised seedlings and 6 weeks-culturednon-excised seedlings. Therefore, we extrapolate that excised cotyledonary stage seedling treated for 3 weeks is sufficient todifferentiate the tolerant germplasms. Using this screening method, we identified the “S81015” could prevail low-temperature stress. Further, we tested grafting compatibility and growth of grafted cucumbers under the low-temperature condition to assess the effect ofrootstock and identify low-temperature tolerant and grafting-compatible rootstock germplasm. Upon grafting, we found a highcorrelation (r=0.97**) between the root fresh weight of 6 weeks-cultured non-excised seedlings and the shoot fresh weight of thegrafted cucumbers. In summary, we could identify the low-temperature tolerant pumpkin germplasms by screening at the earlydevelopmental stage. Further, as a rootstock, the tolerant pumpkins also fortified the low-temperature tolerance of grafted cucumbers.
OsREL2, a rice TOPLESS homolog functions in axillary meristem development in rice inflorescence
권예림,이병하,Si-in Yu,Joo-hyuk Park,Ying Li,Jin-Hee Han,Hemasundar Alavilli,Jung-Il Cho,김태훈,전종성 한국식물생명공학회 2012 Plant biotechnology reports Vol.6 No.3
The morphology of the rice inflorescence,called the panicle, is determined mainly by the activities of axillary meristems including primary, secondary, and spikelet meristems. Recently, in maize, the RAMOSA1ENHANCER LOCUS2 (REL2) gene, orthologous to the Arabidopsis shoot apical meristem fate-determining TOPLESS,was shown to be involved in the regulation of axillary meristem determinacy. In order to investigate the function of the rice REL2 homolog, we identified and characterized the rice REL2 gene (OsREL2). Compared to other rice TPL homologs, OsREL2 gene expression stayed relatively low throughout panicle development. We characterized a T-DNA insertion osrel2 mutant that showed pleiotropic phenotypic defects, such as defects in panicle heading, sterile lemma elongation, and panicle development,suggesting the OsREL2 functions in multiple developmental processes. In particular, osrel2 developed shorter axillary branches and reduced numbers of lateral organs on axillary branches in comparison to the wild-type,indicating that OsREL2 is important in axillary meristem maintenance. Interestingly, osrel2 produced more primary branches and fewer secondary branches than the wild-type. These results suggest that OsREL2 is involved in branch formation regulation, presumably by suppressing primary branch formation and promoting secondary branch formation.