http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Hee-Youl Chai,Seon-Gil Do,Jong-Han Kim,Dong-Seon Kim,Sookyung Sung,Young-Chul Lee,Sung-Sick Woo,Jong-Koo Kang 한국실험동물학회 2009 Laboratory Animal Research Vol.25 No.3
A novel composition of ginsenosides with high Rd and Rg3 content, UG0712 was evaluated for exercise capacity, and post-exercise fatigue recovery through in vivo treadmill maximum running test (MRT) under continuous training (n=9) or resting (n=10) conditions of the SD rats. The standardized ginseng root extract (UG0714) was used for efficacy comparison. Each animal was treated orally at the dose of 25 ㎎/ ㎏ for 8 weeks in exercise group or 9 weeks in resting group. Blood lactate dehydrogenase (LDH) activity and lactic acid concentration were measured to evaluate the post-exercise fatigue recovering effect after exhausted exercise. MRT of UG0712 group showed statistically significant 27.8% increase at week two (P<0.05) and maintained at an increased level in 8-weeks (27.6% increase, not significance) compared with vehicle treated control group. In resting group, MRT of UG0712 after 8-weeks treatment showed 23.0% increase compared with vehicle group (P<0.05) and 23.5% increase when compared with UG0714 group (P<0.05). While no significant changes were found in UG0714 group under either exercise or resting conditions. UG0712 group showed significantly decreased blood LDH levels compared with both vehicle (P<0.01) and UG0714 (P<0.05) groups under trained condition but no significant changes in resting condition. In addition, UG0712 significantly decreased blood lactic acid levels in trained (P<0.01) group, while UG0714 showed no significant changes. Considering the evaluation results from both exercise capability and post-exercise fatigue recovery status, it can be concluded that administration of a standardized Rd and Rg3 ginsenoside composition, UG0712 improves endurance exercise performance probably via increased aerobic energy metabolism, and accelerated post-exercise fatigue recovery.
Antitumor Activity of Pyridazine Derivative in Nude Mice
Chai, Hee-Youl,Sin, Ji-Soon,Kim, Tae Myoung,Kwon, Woon,Cho, Young Min,Choi, Ehn-Kyoung,Hwang, Seock-Yeon,Kim, Yun-Bae,Moon, Aree,Kwon, Soon-Kyoung,Kang, Jong-Koo 德成女子大學校 藥學硏究所 2004 藥學論文誌 Vol.15 No.1
In vitro cytotoxicity and in vivo growth-inhibitory effect of 3-methoxy-6-allylthiopyridazine (K6), an allylthiopyridazine derivative, were evaluated in human hepatocellular carcinoma Hep-G2 cell line and in nude mouse xenograft model, respectively, in comparison with doxorubicin. In vitro cytotoxicity, K6 (5-2,000μM) and doxorubicin (0.05-10μM) decreased tetrazolium conversion by viable cells to formazan in a dose-dependent manner. From a mechanistic study, the Hep-G2 cells exposed to K6 or doxorubicin underwent morphological changes, displaying elongation with filamentous protrusions. In addition, the cells showed chromatin condensation and fragmentation, producing apoptotic bodies. In vivo solid tumor xenograft model, the growth rate of tumor mass was significantly suppressed to the half level by daily oral administration of K6 (20-100mg/kg), which is comparable to the effect of intravenous treatment with doxorubicin (2mg/kg), resulting in the decrease in final tumor weights to 0.78 and 0.50 g by K6 (100mg/㎏) and doxorubicin, respectively, compared with 1.32 g of control. Also, mean survival time of mice of control group (14.4 days) was doubled by treatment with K6 (27.2-29.4 days) or doxorubicin (28.8 days), leading to 100% increase in life span. Interestingly, daily oral treatment with a high dose (100mg/kg) of K6 did not induce testicular toxicity, in contrast to full degenerations of germ cells in atrophic testes intravenously exposed to doxorubicin at 4-6-day intervals, in addition to the emaciation and decrease in body weights of the animals. Taken together, K6, an allylthiopyridazine derivative originated from dially sulfide in garlic oil, could be a promising candidate for chemotherapy of hepatocellular carcinomas as an oral regimen.
Fractional Photothermolytic Effect of Sellas Laser Device on Guinea Pig Skin
Hee-Youl Chai,Soon Sook Nah,Seung Hwan Son,Gyeong Seok Jo,Mi Hyun Kang,Tae Hee Lee,Hyeong Jin Ji,Kwon Pyo Hong,Eui-Tai Lee,Dae Joong Kim,Hyung Geun Song 한국실험동물학회 2009 Laboratory Animal Research Vol.25 No.3
This study was designed to evaluate photothermolytic effects of a fractional laser device and to evaluate healing process after fractional laser treatment on guinea pig skin. Eleven guinea pigs received a single treatment with a prototype diode laser (Sellas<SUP>?</SUP>) emitting a wavelength of 1,550 ㎚, delivering 10 mJ and Excisional biopsies were done immediately after (0 day), 1, 3 and 7 days and analyzed by two blind pathologists using hematoxylin and eosin and Van Gieson stain. Definitive MTZs were observed in both 10 and 20 mJ energy with sparing normal tissue between MTZs. Average depths and diameters of microbeam produced were 308.46, 105.61 ㎛ in 10 mJ and 414.15, 130.57 ㎛ in 20 mJ, respectively. Degeneration of dermal collagen in MTZ is evident, revealing loss of collagen birefringence under polarizing microscope and lack of red staining on Van Gieson stain. One day after fractional laser treatment regeneration of epidermis was completed without disruption of stratum corneum. Microscopic epidermal necrotic debris (MENDs) were noted 1 day on the laser treated epidermis and disappear within 3 days after the treatment. Areas of each microscopic treatment zones were rapidly reduced by replacing newly formed collagen. Repair rate reached 30% at 1 day after laser treatment and at day 7 a wound healing process was almost completed. A single treatment with fractional laser device reveals fractional photothermolytic effects and wound healing responses in both epidermis and dermis.
Antitumor Activity of K6, an Allylthiopyridazine Derivative, in Hep-G2 Cells-transplanted Nude Mice
Hee-Youl Chai,Ji-Soon Sin,Tae Myoung Kim,Woon Kwon,Young Min Cho,Ehn-Kyoung Choi,Seock-Yeon Hwang,Yun-Bae Kim,Jong-Koo Kang 한국실험동물학회 2004 Laboratory Animal Research Vol.20 No.1
In vitro cytotoxicity and in vivo growth-inhibitory effect of 3-methoxy-6-allylthiopyridazine (K6), an allylthiopyridazine derivative, were evaluated in human hepatocellular carcinoma Hep-G2 cell line and in nude mouse xenograft model, respectively, in comparison with doxorubicin. In vitro cytotoxicity, K6 (5-2,000 μM) and doxorubicin (0.05-10 μM) decreased tetrazolium conversion by viable cells to formazan in a dose-dependent manner. From a mechanistic study, the Hep-G2 cells exposed to K6 or doxorubicin underwent morphological changes, displaying elongation with filamentous protrusions. In addition, the cells showed chromatin condensation and fragmentation, producing apoptotic bodies. In vivo solid tumor xenograft model, the growth rate of tumor mass was significantly suppressed to the half level by daily oral administration of K6 (20-100 ㎎/㎏), which is comparable to the effect of intravenous treatment with doxorubicin (2 ㎎/㎏), resulting in the decrease in final tumor weights to 0.78 and 0.50 g by K6 (100 ㎎/㎏) and doxorubicin, respectively, compared with 1.32 g of control. Also, mean survival time of mice of control group (14.4 days) was doubled by treatment with K6 (27.2-29.4 days) or doxorubicin (28.8 days), leading to 100% increase in life span. Interestingly, daily oral treatment with a high dose (100 ㎎/㎏) of K6 did not induce testicular toxicity, in contrast to full degenerations of germ cells in atrophic testes intravenously exposed to doxorubicin at 4-6-day intervals, in addition to the emaciation and decrease in body weights of the animals. Taken together, K6, an allylthiopyridazine derivative originated from dially sulfide in garlic oil, could be a promising candidate for chemotherapy of hepatocellular carcinomas as an oral regimen.