http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Jin, Hanyong,Won, Miae,Shin, Eunkyoung,Kim, Hong-Man,Lee, Kangseok,Bae, Jeehyeon Elsevier 2017 Biochemical and biophysical research communication Vol. No.
<P><B>Abstract</B></P> <P>Pituitary gonadotropins are key hormones that orchestrate the growth and development of ovarian follicles. However, limited information is available on intra-ovarian factors that mediate the actions of gonadotropins. In this study, we identified that the early growth response 2 gene (<I>EGR2</I>) is a gonadotropin-inducible gene in granulosa cells of rats and humans. Analysis of consensus EGR-binding elements (EBEs) showed that the immediate early response 3 gene (<I>IER3</I>) is a novel transcriptional target gene of EGR2 as confirmed by the luciferase assay, electrophoretic mobility-shift assay (EMSA), chromatin immunoprecipitation (ChIP), and western blot analysis. Overexpression of EGR2 promoted survival of KGN human granulosa-derived cells in which IER3 acts as a mediator; knockdown of EGR2 induced death in KGN cells. Additionally, EGR2 was found to regulate the expression of myeloid cell leukemia 1 (MCL-1), which belongs to the BCL-2 family of proteins regulating cell survival. Thus, this study identified a novel signaling axis, comprised of gonadotropins-EGR2-IER3, which is important for the survival of granulosa cells during folliculogenesis.</P>
Vibrotactile cues using tactile illusion for motion guidance
Yeansub Jin,Hanyong Chun,Gi-Hun Yang,Sungchul Kang 제어로봇시스템학회 2013 제어로봇시스템학회 국제학술대회 논문집 Vol.2013 No.10
In this paper, we have developed motion guidance device with phantom sensation and sensory saltation. Phantom sensation and sensory saltation are famous tactile illusion effect for generating spatial and directional information. The device consists of 4 vibrotactile actuators, controller and small battery. It guides the estimated arm motions with vibrotactile cues. We were able to generate continuous vibration with the tactile illusion effect which induces the intentional motion. In order to evaluate the device and motion guidance algorithm, we measured total movement time in visual, acoustic and tactile cues on the elbow joint, forearm rotation and shoulder roll. Implications for the further tactile display method and applications with this device are discussed.
Excimer Emission-Based Fluorescent Probe Targeting Caspase-3
Kim, Tae-Il,Jin, Hanyong,Bae, Jeehyeon,Kim, Youngmi American Chemical Society 2017 ANALYTICAL CHEMISTRY - Vol.89 No.19
<P>A fluorescent probe based on an excimer-forming benzothiazolyl-cyanovinylene (<B>CV</B>) dye was developed to target the apoptotic protease caspase-3. Upon the action of caspase-3, the water-soluble fluorescent probe Ac-DEVD-<B>NH-CV</B>, which is weakly green emissive in aqueous solution, is converted to hydrophobic <B>CV-NH<SUB>2</SUB></B>, which spontaneously aggregates. Aggregation of <B>CV-NH<SUB>2</SUB></B> promotes excimer emission of the <B>CV</B> dye, which allows for the study of caspase-3 activity <I>in vitro</I> and for imaging the activity of the enzyme in living cells because of the large red shift and enhanced fluorescence signal of the probe.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/ancham/2017/ancham.2017.89.issue-19/acs.analchem.7b02790/production/images/medium/ac-2017-02790a_0006.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/ac7b02790'>ACS Electronic Supporting Info</A></P>
Kim, Ka Young,Jin, Hanyong,Park, Jaehyeon,Jung, Sung Ho,Lee, Ji Ha,Park, Hyesong,Kim, Sung Kuk,Bae, Jeehyeon,Jung, Jong Hwa SPRINGER SCIENCE + BUSINESS MEDIA 2018 NANO RESEARCH Vol.11 No.2
<P>Subcellular organelle-specific nanoparticles for simultaneous tumor targeting, imaging, and drug delivery are of enormous interest in cancer therapy. Herein, we report a selective mitochondria-targeting probe 1, which was synthesized by incorporating a triphenyl phosphine with a cyanostilbene and a long alkyl chain moiety. Probe 1 was found to display fluorescence via aggregation-induced emission (AIE). The low molecular-weight cyanostilbene-based probe 1, with and without an anticancer drug, formed a narrow homogeneous nanorod with ca. 110 nm of length or nanoparticles with ca. 20 nm diameter in aqueous media. The self-assembled cyanostilbene nanoparticles (N1) selectively accumulated in the mitochondria of cancer cells and emitted fluorescence. N1 was also able to deliver an anticancer drug, doxorubicin (DOX), to the mitochondria with high efficiency. More importantly, N1 exhibited highly selective cytotoxicity for cancer cells over normal cells. The great potential applications of this self-assembled nanoparticle to biological systems result from its ability to aggregate in the mitochondria. This aggregation led to a significant increase in the generation of intracellular reactive oxygen species and to a decrease in the mitochondrial membrane potential in cancer cells. Furthermore, tumor tissue uptake experiments in mice proposed that the self-assembled N1 had the ability to internalize and deliver the anticancer drug into tumor tissues effectively. Moreover, both N1 and N1-encapsulated doxorubicin (N1-DOX) effectively suppressed tumor growth in a xenograft model in vivo. Taken together, our findings indicate that applications of N1 as a mitochondrial targeting probe, drug delivery platform, and chemotherapeutic agent provide a unique strategy for potential image-guided therapy as well as a site-specific delivery system to cancer cells.</P>
Lee, Ji Ha,Kim, Ka Young,Jin, Hanyong,Baek, Yeong Eun,Choi, Yeonweon,Jung, Sung Ho,Lee, Shim Sung,Bae, Jeehyeon,Jung, Jong Hwa American Chemical Society 2018 ACS APPLIED MATERIALS & INTERFACES Vol.10 No.4
<P>The development of specifically targeted nano particles for subcellular organelles modified with a low molecular-weight organic compound as drug nanocarriers can bring about wide applications in cancer therapy. However, their utility has been hampered by low selectivity, poor biodistribution, and limited efficiency. Herein, we report the aggregation behavior of a triphenylphosphonium-appended coumarin probe (TPP-C) in an aqueous solution and its applications as a mitochondria-targeting probe, and drug delivery carrier, which is a rare example for a low molecular-weight organic compound. The TPP-C formed homogeneous nanoparticles with small diameters in water as well as in mixtures of organic solvents and water. In pure water, the homogeneous nanopartides induced J-aggregation, whereas in mixed solvents, the homogeneous nanoparticles induced H-aggregation. The luminescence intensities of nanoparticles originated from the aggregation-induced emission (ATE) effect in pure water and also in mixtures of organic solvents and water. These findings indicate that the AIE effect of TPP-C was dependent on the solvent. More interestingly, the TPP-C nanoparticles selectively accumulated in mitochondria. The TPP-C nanoparticles alone exhibited noncytotoxicity toward cancer cells. However, with the encapsulation of the anticancer drug doxorubicin (DOX) into the TPP-C nanoparticles, the DOX was efficiently delivered to the mitochondria. These results indicated that the proposed system demonstrates promise as a platform for future clinical medication, particularly for specific suborganelle-targeted drug delivery systems for cancer therapy.</P>