Functional Angioplasty: Definitions, Historical Overview, and Future Perspectives

Hanbit Park,Do-Yoon Kang,Cheol Whan Lee 대한심장학회 2022 Korean Circulation Journal Vol.52 No.1

Percutaneous coronary intervention (PCI) is used to treat obstructive coronary artery disease (CAD). The role of PCI is well defined in acute coronary syndrome, but that for stable CAD remains debatable. Although PCI generally relieves angina in patients with stable CAD, it may not change its prognosis. The extent and severity of CAD are major determinants of prognosis, and complete revascularization (CR) of all ischemia-causing lesions might improve outcomes. Several studies have shown better outcomes with CR than with incomplete revascularization, emphasizing the importance of functional angioplasty. However, different definitions of inducible myocardial ischemia have been used across studies, making their comparison difficult. Various diagnostic tools have been used to estimate the presence, extent, and severity of inducible myocardial ischemia. However, to date, there are no agreed reference standards of inducible myocardial ischemia. The hallmarks of inducible myocardial ischemia such as electrocardiographic changes and regional wall motion abnormalities may be more clinically relevant as the reference standard to define ischemia-causing lesions. In this review, we summarize studies regarding myocardial ischemia, PCI guidance, and possible explanations for similar findings across studies. Also, we provide some insights into the ideal definition of inducible myocardial ischemia and highlight the appropriate PCI strategy.

Synthesis of Ultra-Small Palladium Nanoparticles Deposited on CdS Nanorods by Pulsed Laser Ablation in Liquid: Role of Metal Nanocrystal Size in the Photocatalytic Hydrogen Production

Park, Hanbit,Reddy, D. Amaranatha,Kim, Yujin,Lee, Seunghee,Ma, Rory,Kim, Tae Kyu Wiley - VCH Verlag GmbH & Co. KGaA 2017 Chemistry Vol. No.

Differential Diagnosis of a Left Atrial Mass after Surgical Excision of Myxoma: a Remnant or a Thrombus?

Hanbit Park,Seokjung Jo,Yun Kyung Cho,Jongkwan Kim,Sangcheol Cho,Ju Hyeon Kim,Yeong Jin Jeong,Jae-Kwan Song 대한심장학회 2016 Korean Circulation Journal Vol.46 No.6

1Division of Cardiology, Department of Internal Medicine, 2Department of Internal Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea Echocardiographic diagnosis of atrial myxoma may not always be straightforward, and the distinction between myxoma and thrombi is not easy, especially when we observe a mass after successful surgery. Our report describes a 72-year-old woman who presented with right upper limb hemiparesis and was subsequently diagnosed as having transient ischemic attack due to a left atrial myxoma. One month after successful surgical resection of the tumor, the patient developed left-sided weakness. Echocardiography revealed a left atrial mass attached to the interatrial septum. Intravenous heparin was administered as a therapeutic trial for postoperative thrombi, which resulted in a decrease in mass size within a week. Anticoagulation with warfarin was continued, and complete resolution was demonstrated on a 4-month follow-up transesophageal echocardiography. This case highlights the fact that thrombus formation at the surgical site should be considered an unusual but potential complication after surgical resection of left atrial myxomas.

Binding Specificity of Philyra pisum Lectin to Pathogen-Associated Molecular Patterns, and Its Secondary Structure

Park, Byung Tae,Kim, Byung Sun,Park, Heajin,Jeong, Jaehoon,Hyun, Hanbit,Hwang, Hye Seong,Kim, Ha Hyung The Korean Society of Pharmacology 2013 The Korean Journal of Physiology & Pharmacology Vol.17 No.6

We recently reported a Philyra pisum lectin (PPL) that exerts mitogenic effects on human lymphocytes, and its molecular characterization. The present study provides a more detailed characterization of PPL based on the results from a monosaccharide analysis indicating that PPL is a glycoprotein, and circular dichroism spectra revealing its estimated ${\alpha}$-helix, ${\beta}$-sheet, ${\beta}$-turn, and random coil contents to be 14.0%, 39.6%, 15.8%, and 30.6%, respectively. These contents are quite similar to those of deglycosylated PPL, indicating that glycans do not affect its intact structure. The binding properties to different pathogen-associated molecular patterns were investigated with hemagglutination inhibition assays using lipoteichoic acid from Gram-positive bacteria, lipopolysaccharide from Gram-negative bacteria, and both mannan and ${\beta}$-1,3-glucan from fungi. PPL binds to lipoteichoic acids and mannan, but not to lipopolysaccharides or ${\beta}$-1,3-glucan. PPL exerted no significant antiproliferative effects against human breast or bladder cancer cells. These results indicate that PPL is a glycoprotein with a lipoteichoic acid or mannan-binding specificity and which contains low and high proportions of ${\alpha}$-helix and ${\beta}$-structures, respectively. These properties are inherent to the innate immune system of P. pisum and indicate that PPL could be involved in signal transmission into Gram-positive bacteria or fungi.

The Tumor Suppressor, p53, Negatively Regulates Non-Canonical NF-κB Signaling through miRNA-Induced Silencing of NF-κB-Inducing Kinase

Jang, Hanbit,Park, Seulki,Kim, Jaehoon,Kim, Jong Hwan,Kim, Seon-Young,Cho, Sayeon,Park, Sung Goo,Park, Byoung Chul,Kim, Sunhong,Kim, Jeong-Hoon Korean Society for Molecular and Cellular Biology 2020 Molecules and cells Vol.43 No.1

NF-κB signaling through both canonical and non-canonical pathways plays a central role in immune responses and inflammation. NF-κB-inducing kinase (NIK) stabilization is a key step in activation of the non-canonical pathway and its dysregulation implicated in various hematologic malignancies. The tumor suppressor, p53, is an established cellular gatekeeper of proliferation. Abnormalities of the TP53 gene have been detected in more than half of all human cancers. While the non-canonical NF-κB and p53 pathways have been explored for several decades, no studies to date have documented potential cross-talk between these two cancer-related mechanisms. Here, we demonstrate that p53 negatively regulates NIK in an miRNA-dependent manner. Overexpression of p53 decreased the levels of NIK, leading to inhibition of the non-canonical NF-κB pathway. Conversely, its knockdown led to increased levels of NIK, IKKα phosphorylation, and p100 processing. Additionally, miR-34b induced by nutlin-3 directly targeted the coding sequences (CDS) of NIK. Treatment with anti-miR-34b-5p augmented NIK levels and subsequent non-canonical NF-κB signaling. Our collective findings support a novel cross-talk mechanism between non-canonical NF-κB and p53.

Electrical modulation of a photonic crystal band-edge laser with a graphene monolayer

Kim, Hanbit,Lee, Myungjae,Jeong, Hyunhak,Hwang, Min-Soo,Kim, Ha-Reem,Park, Seondo,Park, Yun Daniel,Lee, Takhee,Park, Hong-Gyu,Jeon, Heonsu The Royal Society of Chemistry 2018 Nanoscale Vol.10 No.18

<P>The electrical control of photonic crystal (PhC) lasers has been an attractive but challenging issue. Laser operation by electrical injection is of key importance for the viability and applicability of the PhC lasers. Another key factor is the electrical modulation of the laser output. The Fermi level of a graphene monolayer can be controlled by electrical gating, which adjusts its optical absorption. In this study, a graphene monolayer sheet is integrated on top of a two-dimensional PhC structure composed of InGaAsP multiple-quantum-wells (MQWs) in order to demonstrate the electrical modulation of a high-power (microwatt-scale) PhC band-edge laser. The introduced dielectric spacer layer presets the delicate balance between the optical gain from the MQWs and optical loss at the graphene monolayer. The proposed device is covered by an ion-gel film, which enables a low-voltage laser modulation at |<I>V</I>g|≤1 V. The modulation is extensively investigated experimentally, and the obtained results are confirmed by performing numerical simulations.</P>

Binding Specificity of Philyra pisum Lectin to Pathogen-Associated Molecular Patterns, and Its Secondary Structure

Byung Tae Park,Byung Sun Kim,Heajin Park,Jaehoon Jeong,Hanbit Hyun,Hye Seong Hwang,Ha Hyung Kim 대한생리학회-대한약리학회 2013 The Korean Journal of Physiology & Pharmacology Vol.17 No.6

We recently reported a Philyra pisum lectin (PPL) that exerts mitogenic effects on human lym-phocytes, and its molecular characterization. The present study provides a more detailed charac-terization of PPL based on the results from a monosaccharide analysis indicating that PPL is a glycoprotein, and circular dichroism spectra revealing its estimated <em>α</em>-helix,<em> β</em>-sheet,<em> β</em>-turn, and random coil contents to be 14.0%, 39.6%, 15.8%, and 30.6%, respectively. These contents are quite similar to those of deglycosylated PPL, indicating that glycans do not affect its intact structure. The binding properties to different pathogen-associated molecular patterns were investigated with hemagglutination inhibition assays using lipoteichoic acid from Gram-positive bacteria, lipopoly-saccharide from Gram-negative bacteria, and both mannan and<em> β</em>-1,3-glucan from fungi. PPL binds to lipoteichoic acids and mannan, but not to lipopolysaccharides or<em> β</em>-1,3-glucan. PPL exerted no significant antiproliferative effects against human breast or bladder cancer cells. These results indicate that PPL is a glycoprotein with a lipoteichoic acid or mannan-binding specificity and which contains low and high proportions of <em>α</em>-helix and<em> β</em>-structures, respectively. These properties are inherent to the innate immune system of <em>P. pisum</em> and indicate that PPL could be involved in signal transmission into Gram-positive bacteria or fungi.

N-glycans and their structural effects on the chicken egg allergen ovotransferrin

Hye Seong Hwang,Heajin Park,Byung Sun Kim,Jaehoon Jeong,Hanbit Hyun,Byung Tae Park,Ho-Young Park,Hee-Don Choi,HaHyung Kim 한국당과학회 2013 한국당과학회 학술대회 Vol.2013 No.1

Ovotransferrin (OT), a multifunctional glycoprotein with defensive and protective activities, accounts for approximately 13% of chicken egg-white proteins and is known to be a major egg-associated allergen along with ovomucoid (OM). In contrast to the well-characterized N-glycans of OM, the N-glycan structure of OT has not been reported. This study used a high-performance liquid chromatography (HPLC) system equipped with a fluorescence detector and mass spectrometric analysis in combination with exoglycosidase digestion to investigate the N-glycan type and branching pattern of OT, and compared them with those of OM. The HPLC peak area was used to calculate the relative amount of each glycan. Seventeen N-glycans were identified, including 11 glycans (1core structure and 10 complex-type oligosaccharides) that are common to both OT and OM. Six characteristic glycans (two truncated structures, one complex-type oligosaccharide, and three hybrid-type oligosaccharides) in OT and eight characteristic glycans in OM were classified. OT contains the following branched complex-type structures : mono- (13.2%), bi- (23.9%), tri- (9.0%), tetra- (2.7%), and penta- (2.8%) antennary oligosaccharides; whereas OM contains mostly tri- (33.5%) and penta- (31.2%) antennary oligosaccharides. The N-glycan-containing bisecting N-acetylglucosamine comprised 43.4% and 79.8% of the total glycans in OT and OM, respectively. Moreover, circular dichroism analysis revealed that the secondary structure of the deglycosylated OT differs markedly from that of the intact protein. To our knowledge this is the first study to compare N-glycans between OT and OM.

차량용 디스커넥터 장치의 제어방법 및 시스템

이한빛(Hanbit Lee),박정우(Jungwoo Park),김경하(Kyungha Kim),백현준(Hyunjun Baek) 한국자동차공학회 2020 한국자동차공학회 학술대회 및 전시회 Vol.2020 No.11

Determining the Reliability of a New Method for Measuring Joint Range of Motion Through a Randomized Controlled Trial

안소영,Hanbit Ko,Jeong Oh Yoon,Sun Ung Cho,Jong Hyun Park,Kang Hee Cho 대한재활의학회 2019 Annals of Rehabilitation Medicine Vol.43 No.6

Objective To compare the reliability and validity of the Korean range of motion standard protocol (KRSP) for measuring joint range of motion (ROM) with those of the conventional ROM measurement using a goniometer. Methods We conducted a randomized controlled trial involving 91 healthy elderly individuals. We compared two strategies of measuring joint ROM to evaluate the reliability and validity of each standardized protocol: first, the KRSP based on the Chungnam National University guidelines and second, handheld goniometric measurement. In the first strategy, 3 examiners (1 rehabilitation doctor, 1 physical therapist, and 1 physical therapy student) independently measured joint ROM in 46 randomly selected subjects; in the second strategy, another 3 examiners (1 rehabilitation doctor, 1 physical therapist, and 1 physical therapy student) measured joint ROM in 45 randomly selected subjects. The reliability of each protocol was calculated using intraclass correlation coefficient, ICC(2,1), and root mean square error (RMSE). Results Both protocols showed good to excellent intra-rater reliability. With goniometer use, the inter-rater reliability was low—ICC(2,1), 95% confidence interval ranged from 0.643 (0.486–0.783) to -0.078 (-0.296–0.494)—and RMSE was high. With the KRSP, the inter-rater reliability ranged from 0.846 (0.686–0.931) to 0.986 (0.972–0.994) and RMSE was low. Conclusion ROM measurements using the KRSP showed excellent reliability. These results indicate that this protocol can be the reference standard for measuring ROM in clinical settings as an alternative to goniometers.