The Effect of Seoritae Extract in Men with Mild to Moderate Lower Urinary Tract Symptoms Suggestive of Benign Prostatic Hyperplasia

Bae, Woong Jin,Park, Hyo Jung,Koo, Hye Cheong,Kim, Do Ram,Ha, U-Syn,Kim, Kang Sup,Kim, Su Jin,Cho, Hyuk Jin,Hong, Sung Hoo,Lee, Ji Youl,Hwang, Sung Yeoun,Kim, Sae Woong Hindawi Publishing Corporation 2016 Evidence-based Complementary and Alternative Medic Vol.2016 No.-

<P>We evaluated the effects of Seoritae extract (SE) on mild to moderate lower urinary tract symptoms (LUTS) suggestive of benign prostatic hyperplasia (BPH). Seventy-six subjects with mild to moderate LUTS suggestive of BPH were prospectively recruited from the urology outpatient clinic and assigned to either SE (4200 mg or 6 tablets 3 times a day) or matching placebo. The primary outcome variable, the International Prostatic Symptom Score (IPSS), was evaluated at baseline and at 4 and 12 weeks. Postvoid residual volume (PVR), maximum urine flow rate (<I>Q</I><SUB>max</SUB>), and prostate-specific antigen (PSA) levels were evaluated. IPSSs decreased significantly from baseline to 12 weeks within the SE group. Significant improvements in IPSS voiding scores at 4 and 12 weeks were also observed in the SE group compared to the placebo group. IPSS storage and quality of life scores were also significantly decreased at 12 weeks in the SE group. There was no change in <I>Q</I><SUB>max</SUB> or PVR in both groups after 12 weeks. Administration of SE for 12 weeks led to significant improvements in LUTS, and it can be concerned as a reasonable and safe alternative for men with mild to moderate LUTS.</P>

Antioxidant and Antifibrotic Effect of a Herbal Formulation <i>In Vitro</i> and in the Experimental Andropause via Nrf2/HO-1 Signaling Pathway

Bae, Woong Jin,Zhu, Guan Qun,Choi, Sae Woong,Jeong, Hyun Cheol,Bashraheel, Fahad,Kim, Kang Sup,Kim, Su Jin,Cho, Hyuk Jin,Ha, U Syn,Hong, Sung Hoo,Lee, Ji Youl,Oh, Hyun-A,Koo, Hye Cheong,Kim, Do Ram,Hw Hindawi 2017 Oxidative medicine and cellular longevity Vol.2017 No.-

<P>The Korean herbal formulation Ojayeonjonghwan is used for improving late-onset hypogonadism (LOH) symptoms such as erectile dysfunction (ED). A previous research suggested that a modified Ojayeonjonghwan (KH-204) could be used as an alternative to the treatment for ED. The pharmacological effects were examined in different conditions, including in vitro and in vivo. We measured the survival rate of TM3 Leydig cells under the oxidative stress condition. The s.c. injection of leuprorelin was used to induce androgen deprivation. We measured serum testosterone levels, oxidative stress, and apoptosis. The results of the treatment by KH-204 (1) preserved TM3 cells from oxidative stress by improving the expression of nuclear factor erythroid 2-related factor 2 (Nrf2)/heme oxygenase-1 (HO-1); (2) lowered the expression of transforming growth factor-beta (TGF-<I>β</I>) 1/SMAD; (3) increased the average of serum testosterone in androgen-deprived male rats; (4) kept the activation of spermatogenesis; (5) upgraded the contents of 8-hydroxy-20-deoxyguanosine (8-OHdG) and degraded the contents of superoxide dismutase (SOD); and (6) reduced apoptosis. We studied that KH-204 improved testicular dysfunction in LOH. It is likely, at least in part, to degrade oxidative stress through the Nrf2/HO-1 pathway. These findings may offer credible evidences for the use of new alternative therapies to treat LOH.</P>

Thermodynamical and Rheological Properties of Wheat Flour Dough Depending on Salt Level

Ha Ram Kim,A-Reum Ryu,Ji-Eun Bae,Ah Leum Kim,Jung Sun Hong,Hee-Don Choi 한국식품영양과학회 2022 한국식품영양과학회 학술대회발표집 Vol.2022 No.10

Biofilm Formation and Cell Surface Properties of Staphylococcus aureus Isolates from Various Sources

Bo-Ram Kim,Young-Min Bae,Jin-Ha Hwang,이선영 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.2

This study investigated biofilm formation, cell surface hydrophobicity, colony spreading, and slime production for 112 Staphylococcus aureus strains isolated from various sources (leaf vegetables, pea leaf, perilla leaf, Kim-bab, person, and animal). When biofilm formation was classified by origin, S. aureus isolated from animal origin showed a significantly higher level of biofilm formation than others (p≤0.05). When S. aureus groups with different levels of biofilm formation (very strong, strong, moderate, and weak) were evaluated for the correlation with cell surface properties, there was a positive correlation between biofilm formation and hydrophobicity (r=0.926). Biofilm formation and colony spreading on tryptic soy broth (without dextrose) also showed positive correlation (r=0.863). In contrast, biofilm formation and slime production were negatively correlated (r= −0.973). Based on these results, the biofilm forming ability of S. aureus differs depending on their origin and might be affected by cell surface properties such as cell surface hydrophobicity.

영역확장법 및 로그격자를 이용한 수치모델링 효율 향상 연구

홍보람(Bo-Ram Hong),배호석(Ho-Seuk Bae),하완수(Wan-Soo Ha),정우근(Woo-Keen Chung) 한국마린엔지니어링학회 2016 한국마린엔지니어링학회지 Vol.40 No.1

Therapeutic effect of a novel histone deacetylase 6 inhibitor, CKD-L, on collagen-induced arthritis in vivo and regulatory T cells in rheumatoid arthritis in vitro

Oh, Bo Ram,Suh, Dong-hyeon,Bae, Daekwon,Ha, Nina,Choi, Young Il,Yoo, Hyun Jung,Park, Jin Kyun,Lee, Eun Young,Lee, Eun Bong,Song, Yeong Wook BioMed Central 2017 Arthritis research & therapy Vol.19 No.-

<P><B>Background</B></P><P>Histone deacetylase (HDAC) inhibitor has recently been reported to have a therapeutic effect as an anti-inflammatory agent in collagen-induced arthritis (CIA). We investigated the therapeutic effect of a new selective HDAC6 inhibitor, CKD-L, compared to ITF 2357 or Tubastatin A on CIA and regulatory T (Treg) cells in patients with rheumatoid arthritis (RA).</P><P><B>Methods</B></P><P>CIA was induced by bovine type II collagen (CII) in DBA/1 J mice. Mice were treated with HDAC inhibitor for 18 days. Arthritis score was assessed and histological analysis was performed by hematoxylin and eosin (H&E) stain. Cytotoxic T-lymphocyte associated protein (CTLA)-4 expression in induced Treg cells was analyzed and suppression assay was analyzed using Treg cells and effector T (Teff) cells isolated from naive C57BL/6 mice by flow cytometry. Cytokines were analyzed in peripheral blood mononuclear cells (PBMC) of five patients with RA by enzyme-linked immunosorbent assay (ELISA) and real-time polymerase chain reaction (PCR). Tumor necrosis factor (TNF) was analyzed using PMA- activated THP-1 cells by ELISA. Suppression assay was analyzed using Treg cells and Teff cells isolated from RA patients by flow cytometry.</P><P><B>Results</B></P><P>In the CIA model, CKD-L and Tubastatin A significantly decreased the arthritis score. CKD-L increased CTLA-4 expression in Foxp3<SUP>+</SUP> T cells and inhibited the proliferation of Teff cells in the suppression assay. In RA PBMC, CKD-L significantly inhibited TNF and interleukin (IL)-1β, and increased IL-10. CKD-L and Tubastatin A inhibited TNF secretion from PMA-activated THP-1 cells. CKD-L and ITF 2357 inhibited the proliferation of Teff cells in RA patients in the suppression assay. Tubastatin A had no effect on inhibition of proliferation.</P><P><B>Conclusion</B></P><P>CKD-L decreased the arthritis score in CIA, reduced the expression of TNF and IL-1β, and increased the expression of IL-10 in PBMC from RA patients. CKD-L increased CTLA-4 expression and the suppressive function of Treg cells. These results suggest that CKD-L may have a beneficial effect in the treatment of RA.</P>

6-sialyllactose ameliorates dihydrotestosterone-induced benign prostatic hyperplasia through suppressing VEGF-mediated angiogenesis

( Eun-yeong Kim ),( Bo-ram Jin ),( Tae-wook Chung ),( Sung-jin Bae ),( Hyerin Park ),( Dongryeol Ryu ),( Ling Jin ),( Hyo-jin An ),( Ki-tae Ha ) 생화학분자생물학회(구 한국생화학분자생물학회) 2019 BMB Reports Vol.52 No.9

Benign prostatic hyperplasia (BPH), a common disease in elderly males, is accompanied by non-malignant growth of prostate tissues, subsequently causing hypoxia and angiogenesis. Although VEGF-related angiogenesis is one of the therapeutic targets of prostate cancer, there is no previous study targeting angiogenesis for treatment of BPH. Dihydrotestosterone (DHT)- induced expressions of vascular endothelial growth factor (VEGF) in prostate epithelial RWPE-1 cells and human umbilical vascular endothelial cells (HUVECs). Conditioned media (CM) from DHT-treated RWPE-1 cells were transferred to HUVECs. Then, 6SL inhibited proliferation, VEGFR-2 activation, and tube formation of HUVECs transferred with CM from DHT-treated RWPE-1 cells. In the rat BPH model, 6SL reduced prostate weight, size, and thickness of the prostate tissue. Formation of vessels in prostatic tissues were also reduced with 6SL treatment. We found that 6SL has an ameliorative effect on in vitro and in vivo the BPH model via inhibition of VEGFR-2 activation and subsequent angiogenesis. These results suggest that 6SL might be a candidate for development of novel BPH drugs. [BMB Reports 2019; 52(9): 560-565]

Trehalose biosynthesis gene otsA protects against stress in the initial infection stage of the Burkholderia-bean bug symbiosis

Junbeom Lee,Bohyun Jeong,Ha Ram Bae,Ho Am Jang,Jiyeun Kate Kim 한국응용곤충학회 2023 한국응용곤충학회 학술대회논문집 Vol.2023 No.04

Humulus japonicus extract exhibits antioxidative and anti-aging effects via modulation of the AMPK-SIRT1 pathway

SUNG, BOKYUNG,CHUNG, JI WON,BAE, HA RAM,CHOI, JAE SUE,KIM, CHEOL MIN,KIM, NAM DEUK Spandidos Publications 2015 Experimental and therapeutic medicine Vol.9 No.5

<P>The perennial herb, Humulus japonicus, has been previously described as possessing potential antituberculosis and anti-inflammatory properties. In the present study, the anti-aging activity of ethanol extracts from the leaves of H. japonicus (HJE) was evaluated in yeast and human fibroblast cells. In addition, the antioxidant activity of HJE was analyzed using free radical scavenging assays. Furthermore, the mechanism underlying the hypothesized HJE-associated extension of lifespan was investigated, and the results indicated that HJE was able to extend the lifespan of yeast cells. Further experiments demonstrated that HJE upregulated the longevity-associated proteins, sirtuin 1 and AMP-activated protein kinase, and effectively inhibited the generation of reactive oxygen species (ROS). In addition, the antioxidative potential of the active constituents of HJE, including luteolin, luteolin 7-glycoside, quercetin and quercitrin, was evaluated and the results demonstrated that these flavonoids were able to scavenge ROS in cell-free and intracellular systems. In summary, the results revealed that HJE possessed the potential for antioxidative activity; however, further in vivo investigations are required with the aim of developing safe, high-efficacy anti-aging agents.</P>

일반논문 : 헌법재판과 사회과학적 연구의 활용; 소수자 인권의 관점에서

권민지 ( Min Ji Kwon ),배정훈 ( Jeong Hun Bae ),장윤호 ( Yoon Ho Chang ),조성제 ( Seong Je Jo ),하보람 ( Bo Ram Ha ) 서울대학교 BK21 법학연구단 공익인권법연구센터 2013 공익과 인권 Vol.13 No.-

헌법재판소의 결정에 사회과학적 논의들을 반영하여 설득력을 높여야 한다는 주장은 2000년대 이후로 지속적으로 제기되어 왔다. 그러나 이와 관련한 기존 연구들은 헌법재판 과정에서 사회과학적 논증과 변론의 유용성을 탐구하거나 헌법재판소의 결정례를 사회과학적 연구의 대상으로 삼는 데 그친다. 헌법재판제도의 개선과 발전을 모색하기 위해서는 사회과학적 논의의 활용이 실제 헌법재판과정에서 소수자의 인권 보장에 어떻게 기여할 수 있을지 탐색할 필요가 있다. 헌법재판은 기본적으로 국가권력으로부터 헌법이 보장하는 각 개인의 고유한 기본권이 침해당하지 않도록 공권력을 통제하는 제도다. 헌법재판소는 사법작용을 하는 ‘비다수파 기관(non-majoritarian institution)’으로서 소수의 의견에 충분히 귀를 기울일 수 있고, 다수가 만들어낸 법률에 대한 위헌심사권을 가지고 있기 때문에 소수자의 인권을 보호하는 데 가장 적합한 헌법기관이다. 그러므로 헌법은 헌법재판의 본질 또는 목적을 소수자와 사회적 약자의 기본적 인권을 보장하는 데 두고 있다고 볼 수 있다. 그런데 현행 헌법재판에서는 이러한 본질을 달성할 수 없게 하는 문제들이 복합적으로 작동하고 있다. 이 글에서는 헌법재판소의 현행 운영상 발견되는 세 가지 차원의 한계들이 헌법재판제도가 소수자 인권을 보호하는 데 장애물로 기능하고 있음을 보이고자 한다. 세 가지 차원의 한계란, 첫째, 논증의 불성실함에서 비롯되는 논리적 한계, 둘째, 헌법재판관들의 인권과 소수자 문제에 대한 이해의 부족에서 나오는 내재적 한계, 마지막으로 권력분립원칙과 민주적 정당성의 부재라는 외재적 한계이다. 이러한 한계들을 극복하기 위해서는 기본적으로 소수자 문제에 관한 사회과학적 논의를 활용하여 논증의 성실함을 높이고, 재판관들의 소수자 인권문제에 대한 주의를 환기시켜야 한다. 더 나아가 결정과정에 실질적인 변화를 불러오기 위해서는 객관규범과 주관규범을 구분하고 구체적 주관과 추상적 주관을 분리하는 새로운 논증과정, 분리논증을 채택할 필요가 있다. 분리논증과 사회과학적 논의가 헌법재판에 폭넓게 반영된다면, 헌법재판소는 본질적 사명인 소수자와 사회적약자의 인권보장에 더욱 가까워질 수 있을 것이다. Arguments that the Consitutional Court`s decision should embrace or reflect social-scientific findings have continuously been raised since the 2000s. However, previous studies have been limited to researching the usefulness of social-scientific arguments and pleading in the course of the constitutional trial, or to merely treating the constitutional court`s decisions as a research subject. In order to seek progress and improvement of constitutional court system, there is a pressing need to study how the utilization of social-scientific studies can contribute to the protection of rights of minorities. The constitutional trial is basically a system to control governmental force, thereby preventing the violation of constitutional rights of each and every individuals. The Constitutional Court is the most suitable constitutional institution for protecting rights of minorities: as a non-majoritarian institution, it is capable of remaining sensitive and responsive to the needs of the minorities, and it is also authorized to rule on the constitutionality of laws legislated by the majority. Therefore, it can be argued that the essence or aim of the Consitutional Court lies in the protection of basic rights of minorities and the socially disadvantaged. However, under the current constitution trial, there are multiple and complex obstacles that threaten the achievement of such fundamental aims of the Court. This paper will discuss that three levels of limitations function as obstacles to the protection of the rights of minorities, under the current operation of the Constitutional Court. First, there is a limitation on the logical level, which arises as a result of insufficient or insincere argumentation. Second, limitation exists on an internal level as judges lack understanding on the issues of human rights and minorities. Lastly, external limitation arises from the absence of democratic legitimacy and division of powers. In order to overcome such limitations, it is necessary to utilize social-scientific arguments in strengthening and improving the judges` argumentation. Moreover, it is also necessary to call the constitutional judges` attention to the issues of minorities and human rights. Furthermore, a new reasoning method, separate-reasoning, which separates objective and subjective norms and divides subjective and abstract processes, needs to be adopted in order to induce substantial changes in the decision-making process, If separation-reasoning and social-scientific discussions become broadly reflected in the constitutional trial, the Constitutional Court will be closer to ensuring the rights of minorities and the underprivileged.