WS-5 Extract of <i> Curcuma longa, Chaenomeles sinensis,</i> and <i> Zingiber officinale</i> Contains Anti-AChE Compounds and Improves <i>β</i> -Amyloid-Induced Memory Impairment in Mice

Kim, Ju Eun,Shrestha, Abinash Chandra,Kim, Hyo Shin,Ham, Ha Neul,Kim, Jun Hyeong,Kim, Yeong Jee,Noh, Yun Jeong,Kim, Su Jin,Kim, Dae Keun,Jo, Hyung Kwon,Kim, Dae Sung,Moon, Kwang Hyun,Lee, Jeong Ho,Jeo Hindawi 2019 Evidence-based Complementary and Alternative Medic Vol.2019 No.-

<P>Alzheimer's disease (AD) is linked to an extensive neuron loss via accumulation of amyloid-beta (A<I>β</I>) as senile plaques associated with reactive astrocytes and microglial activation in the brain. The objective of this study was to assess the therapeutic effect of WS-5 ethanol extract in vitro and in vivo against A<I>β</I>-induced AD in mice and to identify the extract's active constituents. In the present study, WS-5 exerted a significant inhibitory effect on acetylcholinesterase (AChE). Analysis by transmission electron microscopy (TEM) revealed that WS-5 prevented A<I>β</I> oligomerization via inhibition of A<I>β</I><SUB>1-42</SUB> aggregation. Evaluation of antioxidant activities using 1, 1-diphenyl-2-picrylhydrazyl (DPPH) demonstrated that WS-5 possessed a high antioxidant activity, which was confirmed by measuring the total antioxidant status (TAS). Furthermore, the anti-inflammatory properties of WS-5 were examined using lipopolysaccharide-stimulated BV-2 microglial cells. WS-5 significantly inhibited the lipopolysaccharide–induced production of nitric oxide and two proinflammatory cytokines, TNF-<I>α</I> and IL-6. The memory impairment in mice with A<I>β</I>-induced AD was studied using the Morris water maze and passive avoidance test. Immunohistochemistry was performed to monitor pathological changes in the hippocampus and cortex region of the mouse brain. The animal study showed that WS-5 (250 mg/kg) treatment improved learning and suppressed memory impairment as well as reduced A<I>β</I> plaque accumulation in A<I>β</I>-induced AD. HPLC analysis identified the extract's active compounds that exert anti-AChE activity. In summary, our findings suggest that WS-5 could be applied as a natural product therapy with a focus on neuroinflammation-related neurodegenerative disorders.</P>

[PB-0087] Efficiency Analysis of Agrobacterium Transformation of Korean Wheat Varieties By GUS System

Ha Neul Lee(Ha Neul Lee),Geon Hee Lee(Geon Hee Lee),Jae Yoon Kim(Jae Yoon Kim) 한국육종학회 2022 한국육종학회 공동학술발표집 Vol.2022 No.-

편측 요통을 호소하는 환자에 있어서 척추 주위 근육의 지방량과 통증과의 관계

김하늘 ( Ha Neul Kim ),김경훈 ( Kyoung Hun Kim ),김주원 ( Joo Won Kim ),진은석 ( Eun Seok Jin ),하인혁 ( In Hyuk Ha ),고동현 ( Dong Hyun Koh ),홍순성 ( Soon Sung Hong ),권혁준 ( Hyeok Joon Kwon ) 한방재활의학과학회 2009 한방재활의학과학회지 Vol.19 No.1

목적: 요통은 임상에서 가장 흔하게 접할 수 있는 질환이며, 동시에 통증으로 인한 근무 결손도나 생활 불편감이 높은 질병이다. 그래서, 많은 연구들이 요통을 일으키는 원인 혹은 악화시키는 유발 인자를 밝혀내려는데 초점이 맞추어져 있다. 이번 연구의 목적은 편측 요통을 호소하는 환자에 있어서 MRI에서 조사된 척추 주위 근육의 지방량과 통증과의 관계에 대해서 알아보는데 있다. 방법: 2007년 11월부터 2008년 4월까지 본원에 요통으로 내원한 환자를 대상으로, 편측 요통을 호소하며, 체질량 지수가 25kg/㎡ 이하이며, 만 20세이상 40세미만인 환자 24명을 대상으로 연구를 진행하였다. MRI에서 조사되어진 L4-5 디스크 레벨의 척추 주위 근육의 지방량을 객관적인 pseudocoloring technique을 이용해 측정하고, paired t-test로 결과를 분석하여 요통이 유발된 측과 지방량의 관계에 대해서 연구하였다. 결과: 요통이 있는 측의 지방량과 요통이 없는 측의 지방량을 측정하여 데이터를 분석하였다. 양측의 지방량과 통증의 유무에 대한 관계는 통계적으로 유의한 수치를 나타냈다(P <0.05). 요통이 있는 측과 없는 측과의 성별, 나이, BMI의 통계적인 차이는 없었으며, 지방량 측정시 남자들이 여자들에 비해 지방량이 더 높게 측정되었다(남자 8.5±5.1%, 여자 6.5±3.6%). 결론: 이 연구를 통해 척추 주위 근육의 지방량과 요통에 대한 상관성의 증거를 제시하였다. 잘 선별되어진 환자의 MRI를 통해 객관적인 방법으로 지방량을 체크를 하였으며, 추후 더 많은 개체수를 이용해 보다 더 정확한 관련성을 파악하고, 나아가 지방량에 따른 통증 강도의 상관성에 대해서 연구가 필요하리라 생각된다. Objectives: Low back pain(LBP) is a common disabling disease in clinical practice and loss of working hours due to this condition is huge. The aim of this study was to determine if there was an association between fat deposit of paraspinal muscles as observed on MRI scans in patients presenting with unilateral LBP. Methods: 24 patients who visiting our hospital with a clinical presentation of unilateral LBP were recruited to the study. Patients were between 20 and 30 years and had a history of unilateral LBP within 12 months. After MRI scaning, the images were saved in DICOM file format for Picture Archiving and Communication System(PACS). The percentage of fat infiltrated area was measured using a pseudocoloring technique. Data were analyzed comparing the fat deposits of the muscles on the symptomatic and asymptomatic sides. Paired t-test was used to find the difference between the measurements of fat tissue in individual patients. Results: The amount of fat in the symptomatic side was 7.6±4.51%, asymptomatic side was 6.7±4.29%. There were increases, statistically significant, in the fat changes of the paraspinal muscles at the L4-5 disc level(P <0.05). Also, men were likely than women to have more fat deposit in symptomatic side(men 8.5±5.1%, women 6.5±3.6%). Conclusions: The amount of fat in the symptomatic side shows significantly increased than asymptomatic side in the paraspinal muscles at the L4-5 disc level. It suggested that fat infiltration in the muscles associated with LBP. Further studies will be needed to confirm the relationship between the muscle fatty changes and LBP in the large sample size. In addition, the correlation of pain severity with fat infiltration needs to be addressed.

Feasibility of Hounsfield Unit Values in Diagnosis of Fatty Liver

( Ha Neul Kim ),( Hei Gwon Choi ),( In Sun Kwon ),( Woo Sun Ryu ),( Seok Hyun Kim ),( Byung Seok Lee ),( Hyuk Soo Eun ) 대한간학회 2021 춘·추계 학술대회 (KASL) Vol.2021 No.1

Recombinant Human Acidic Fibroblast Growth Factor (aFGF) Expressed in Nicotiana benthamiana Potentially Inhibits Skin Photoaging

Ha, Jang-Ho,Kim, Ha-Neul,Moon, Ki-Beom,Jeon, Jae-Heung,Jung, Dai-Hyun,Kim, Su-Jung,Mason, Hugh,Shin, Seo-Yeon,Kim, Hyun-Soon,Park, Kyung-Mok Georg Thieme Verlag KG 2017 Planta medica Vol.83 No.10

<B>Abstract</B><P>Responding to the need for recombinant acidic fibroblast growth factor in the pharmaceutical and cosmetic industries, we established a scalable expression system for recombinant human aFGF using transient and a DNA replicon vector expression in Nicotiana benthamiana. Recombinant human-acidic fibroblast growth factor was recovered following Agrobacterium infiltration of N. benthamiana. The optimal time point at which to harvest recombinant human acidic fibroblast growth factor expressing leaves was found to be 4 days post-infiltration, before necrosis was evident. Commassie-stained SDS-PAGE gels of His-tag column eluates, concentrated using a 10 000 molecular weight cut-off column, showed an intense band at the expected molecular weight for recombinant human acidic fibroblast growth factor. An immunoblot confirmed that this band was recombinant human acidic fibroblast growth factor. Up to 10 µg recombinant human-acidic fibroblast growth factor/g of fresh leaves were achieved by a simple affinity purification protocol using protein extract from the leaves of agroinfiltrated N. benthamiana. The purified recombinant human acidic fibroblast growth factor improved the survival rate of UVB-irradiated HaCaT and CCD-986sk cells approximately 89 and 81 %, respectively. N. benthamiana-derived recombinant human acidic fibroblast growth factor showed similar effects on skin cell proliferation and UVB protection compared to those of Escherichia coli-derived recombinant human acidic fibroblast growth factor. Additionally, N. benthamiana-derived recombinant human acidic fibroblast growth factor increased type 1 procollagen synthesis up to 30 % as well as reduced UVB-induced intracellular reactive oxygen species generation in fibroblast (CCD-986sk) cells.</P><P>UVB is a well-known factor that causes various types of skin damage and premature aging. Therefore, the present study demonstrated that N. benthamiana-derived recombinant human acidic fibroblast growth factor effectively protects skin cell from UVB, suggesting its potential use as a cosmetic or therapeutic agent against skin photoaging.</P>

Electroacupuncture Potentiates the Antiallodynic Effect of Intrathecal Neostigmine in a Rat Model of Neuropathic Pain

Kim, Ha Neul,Park, Jung Hyuk,Kim, Sun Kwang,Sun, Boram,Koo, Sungtae,Choi, Sun Mi,Bae, Hyunsu,Min, Byung-Il The Physiological Society of Japan 2008 JOURNAL OF PHYSIOLOGICAL SCIENCES Vol.58 No.5

<P>This study was performed to examine whether electroacupuncture potentiates the neostigmine-induced antiallodynia in neuropathic pain rats. Although intrathecal neostigmine (0.05, 0.1, and 0.3 μg) dose-dependently relieved cold allodynia, 0.3 μg neostigmine caused side effects. The coapplication of 0.1 μg neostigmine and electroacupuncture, however, produced potent antiallodynia, which was parallel to the effect of 0.3 μg neostigmine, without side effects. These results indicate that electroacupuncture can enhance the antiallodynic action of intrathecal neostigmine.</P>

Renoprotective and antioxidant effects of Saururus chinensis Baill in rats fed a high-fructose diet

Ha-Neul Choi,Yong-Hyun Park,Ji-Hye Kim,Min-Jung Kang,Soo-Mi Jeong,Hyeon Hoe Kim,Jung-In Kim 한국영양학회 2011 Nutrition Research and Practice Vol.5 No.4

This study investigated the preventive effect of Saururus chinensis Baill against renal damage induced by a high-fructose diet in rats. The rats (n = 30) were fed either a cornstarch-based (65%), high-fructose (65%), or high-fructose (64.5%) diet with 0.5% S. chinensis Baill extract for 10 weeks. Twenty-four hour urine collections were obtained and the animals were sacrificed after an overnight fast. Serum urea and creatinine and urine albumin were measured using colorimetric methods, and creatinine clearance was determined. In addition, thiobarbituric acid reactive substances (TBARS), reduced glutathione (GSH), and the activity of superoxide dismutase (SOD) in the kidney were determined. Kidney samples were also examined histologically. The fructose-fed rats showed renal dysfunction, indicated by decreased creatinine clearance, increased albumin in the urine, and increased urea and creatinine in the serum. These renal function parameters were comparable to control levels in rats that consumed S. chinensis Baill. Fructose consumption increased renal TBARS and reduced GSH and SOD activity, whereas these levels were near-normal in the rats consuming S. chinensis Baill. The kidneys of fructose-fed rats showed glomerular basement membrane thickening, mesangial matrix expansion, and tubule dilation. These pathological changes were not seen in the rats that consumed S. chinensis Baill. Therefore, S. chinensis Baill effectively alleviated fructose-induced renal damage in these rats, at least partially due to antioxidant activity.

Triallyl Borate as an Effective Separator/Cathode Interphase Modifier for Lithium-ion Batteries

Ha Neul Kim,Hye Rim Lee,Taeeun Yim The Korean Electrochemical Society 2023 Journal of electrochemical science and technology Vol.14 No.3

Ni-rich layered oxides cathode has recently gained attention as an advanced cathode material due to their applicable energy density. However, as the Ni component in the layered site is increased, the high reactivity of Ni⁴⁺ results in parasitic reaction associated with decomposing electrolyte, which leads to a rapid decreasing the lifespan of the cell. The electrolyte additive triallyl borate (TAB) improves interfacial stability, leading to a stable cathode-electrolyte interphase (CEI) layer on the LNCM83 cathode. A multi-functionalized TAB additive can produce a uniformly distributed CEI layer via electrochemical oxidation, which implies an increase in long-term cycling performance. After 100 cycles at elevated temperature, the cell tested by 0.75 TAB retained 88.3% of its retention ratio, whereas the cell performed by TAB-free electrolyte retained 64.1% of its retention. Once the TAB additive formed CEI layers on the LNCM83 cathode, it inhibited the decomposition of carbonate-based solvents species in addition to the dissolution of transition metal components from the cathode. The addition of TAB to LNCM83 cathode material is believed to be a promising way to increase the electrochemical performance.

The catalytic role of additive components for the nitridation of silicon/additive mixture

KIM, Ha-Neul,PARK, Young-Jo,KIM, Jin-Myung,LEE, Jae-Wook,KO, Jae-Woong,KIM, Hai-Doo,LEE, Seung-Jun,BAEK, Seung-Su,SEO, Il-Sung Ceramic Society of Japan 2016 JOURNAL OF THE CERAMIC SOCIETY OF JAPAN - Vol.124 No.3

<P>The nitriding behavior of silicon/additives mixture with varied composition was investigated by focusing on the catalytic effect of sintering additives (Y2O3 and Al2O3) and diluents (Si3N4). While sintering additives have originally been mixed to form a liquid phase to promote both densification and grain growth during post-sintering, it was revealed that the addition of Y2O3 enhances the nitriding reaction by eliminating the SiO2 and Si3N4 layer on the surface of silicon particles. Further, a synergistic effect resulting in an increased nitridation was verified by concurrent addition of sintering additives and diluents. The contribution of each component of the compact to the promoted nitriding reaction was elucidated in quasi in-situ manner by the combination of phase analysis and thermogravimetric analysis. (C) 2016 The Ceramic Society of Japan. All rights reserved.</P>

Characterization of porous sintered reaction-bonded silicon nitride containing three different rare-earth oxides

Kim Ha-Neul,Kim Mi-Ju,Park Young-Jo,Ko Jae-Woong 한국세라믹학회 2021 한국세라믹학회지 Vol.58 No.1

The eff ect of rare-earth oxides on the characteristics of porous sintered reaction-bonded silicon nitride (SRBSN) was investigated. Three types of raw Si powder mixtures containing diff erent rare-earth oxide (La 2 O 3 , Er 2 O 3 , and Yb 2 O 3 ) were prepared and nitrided in the form of compacts. The nitriding profi les of the respective raw powder mixtures with elevating temperature indicated that Yb 2 O 3 clearly promoted the nitridation of Si compacts at low temperature compared with other rare-earth oxides, and the β-Si 3 N 4 ratio after completion of the nitriding reaction was diff erent at which temperature the major nitridation occurred. Yb 2 O 3 was found to be the most eff ective additive to achieve a strong porous SRBSN, having the fl exural strength of 441 MPa. The reason why Yb 2 O 3 promotes the nitridation reaction and has excellent mechanical properties after post-sintering is that oxygen was removed during the nitriding reaction, which was supported by the analysis of oxygen content via the laser-induced breakdown spectroscopy.