p.R72P, PIN3 Ins16bp Polymorphisms of TP53 and CCR5Δ32 in North Indian Breast Cancer Patients

Guleria, Kamlesh,Sharma, Sarika,Manjari, Mridu,Uppal, Manjit Singh,Singh, Neeti Rajan,Sambyal, Vasudha Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.7

Background: The present study aimed to find the prognostic implications of two polymorphisms in TP53 (p.R72P, PIN3 Ins16bp) and one in CCR5 ($CCR5{\Delta}32$) in sporadic breast cancer patients. Methods: DNA samples of 80 breast cancer patients and 80 age and gender matched unrelated healthy control individuals from Punjab, North West India were analyzed. Results: For p.R72P, the genotype frequency was 13.8% (RR), 58.8% (RP), 27.5% (PP) in patients and 33.9% (RR), 40.0% (RP), 26.5% (PP) in controls. For PIN3 Ins16bp, the genotype frequencies were 53.75% (A1A1), 37.5% (A1A2), 8.75% (A2A2) in patients and 66.3% (A1A1), 31.3% (A1A2), 2.5% (A2A2) in controls. Only 4 (5%) breast cancer patients were heterozygous for $CCR5{\Delta}32$ deletion. Common RR-A1A1-WT/WT genotype was lower while RP-A1A2-WT/WT genotype was higher in patients as compared to controls. RP-A1A1-WT/WT genotype was significantly higher in patients as compared to control individuals (p = 0.008). Conclusion: Though a clear association of any particular genotype with sporadic breast cancer or stage was not apparent, the results of present study were suggestive that sporadic breast cancer patients with RR-A1A1-WT/WT genotype might have a better response to chemotherapy, thus improving their chances of survival.

Impact of VEGFA promoter polymorphisms on esophageal cancer risk in North-West Indians: a case-control study

Guleria Kamlesh,Kaur Simranjot,Mahajan Deepanshi,Sambyal Vasudha,Sudan Meena,Uppal Manjit Singh 한국유전학회 2022 Genes & Genomics Vol.44 No.8

Background: Angiogenesis play a critical role in the development and progression of tumors in solid tumors. Vascular endothelial growth factor (VEGF) is one of the most important endothelial cell mitogen which plays a critical role in normal physiological and tumor angiogenesis. Objectives: The objective of this case-control study was to investigate the association of VEGF-2578C/A, -2549 I/D, and -460T/C promoter polymorphisms with esophageal cancer risk in North-West Indians. Methods: In this study, 200 sporadic esophageal cancer patients and 200 healthy, unrelated, age and gender matched controls were analyzed. The genomic DNA was extracted from blood samples using phenol chloroform method. Genotyping of VEGF- 2549I/D polymorphism was carried out by direct polymerase chain reaction (PCR) whereas VEGF -2578C/A and VEGF-460T/C) polymorphisms were analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Results: AA genotype (p = 0.005) and A allele (p = 0.005) VEGF -2578 C/A, II genotype (p = 0.011) and I allele (p = 0.012) of VEGF - 2549 I/D and CC genotype (p = 0.013) and C allele of VEGF-460T/C polymorphisms were significantly associated with increased risk of esophageal cancer. Stratification of data on the basis of gender showed that VEGF -2578 AA genotype (p = 0.001) and A allele (p = 0.001); VEGF -2549 II genotype (p = 0.002) and I allele (p = 0.002) and VEGF- 460CC genotype (p = 0.001) and C allele (p = 0.002) was significantly associated with increased risk of esophageal cancer in female group. Haplotype analysis revealed that A-2578 I- 2549 C- 460 haplotype was significantly associated with increased risk for esophageal cancer in total samples (p = 0.008) as well as in female group (p = 0.001). Conclusions: The results of present study indicate that VEGF -2578C/A, - 2549I/D and -460T/C polymorphisms were significantly associated with increased risk of esophageal cancer in North-West Indians.

Analytical parameters to check association between load carriage system and bag packs-influenced musculoskeletal complications among school going students: a scoping review

Mahima Guleria,Shashi Prakash Sharma,Amit Kumar 한국운동영양학회 2023 Physical Activity and Nutrition (Phys Act Nutr) Vol.27 No.3

[Purpose] This study aimed to investigate the correlation between load-carriage systems and backpacks on musculoskeletal complications among school-going students. Additionally, the purpose was to identify research gaps and provide recommendations for future research. [Methods] To conduct this study, we conducted a thorough search of five databases for literature published between January 2010 and December 2022. Our focus was on original published articles that reported musculoskeletal complications using analytical parameters or outcome measures among school-going students. Two reviewers independently extracted data for eligibility. The studies selected for the analysis focused on musculoskeletal complications and the outcome measures to report these complications. [Results] A total of 3541 titles were screened; of which, 18 observational studies were included. The musculoskeletal complications identified in these studies included lower back, shoulder, and neck pain and disturbed posture. Overall, the quality of the reporting in these studies was deemed satisfactory. [Conclusion] Our analysis revealed that pain and postural abnormalities were the most frequently reported complications. Hence, we recommend that future studies incorporate multiple evaluations rather than concentrating on a single symptom.

A novel approach for prediction of surface roughness in turning of EN353 steel by RVR-PSO using selected features of VMD along with cutting parameters

Vikrant Guleria,Vivek Kumar,Pradeep K. Singh 대한기계학회 2022 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.36 No.6

The abrupt changes in tool-workpiece interaction during machining process induce variation in the surface quality of work material. These interactions include built-up edge formation and their break-off, environmental conditions (use of coolant, rise of temperature etc.), material imperfections, improper structural fitness of machine & tool components, etc. This study presents prediction of surface roughness in turning of EN353 steel implementing the variational mode decomposition (VMD) for processing the vibration data, followed by estimation of the surface roughness using the relevance vector regression (RVR) optimized by particle swarm optimization (PSO). The raw vibration data has been decomposed in five discrete sets of frequency components known as variational mode functions (VMFs). A set of twenty-one statistical features in each three axes have been extracted for raw data and each VMF. The RVR has been trained using these 21×3 = 63 features and 3 cutting parameters – cutting speed, feed depth of cut. The RVR has also been trained separately using top 5 features selected through RreliefF algorithm. The optimal decomposition level has been determined to minimize the noise and predict the surface finish accurately. The results obtained in 1st VMF (high frequency, low amplitude) using its top 5 features for prediction have been found to be reliable with higher prediction accuracy.

Mapping of Brain Activations to Rectal Balloon Distension Stimuli in Male Patients with Irritable Bowel Syndrome Using Functional Magnetic Resonance Imaging

( Anupam Guleria ),( Arun Karyampudi ),( Rajan Singh ),( Chunni L Khetrapal ),( Abhai Verma ),( Uday C Ghoshal ),( Dinesh Kumar ) 대한소화기기능성질환·운동학회(구 대한소화관운동학회) 2017 Journal of Neurogastroenterology and Motility (JNM Vol.23 No.3

Background/Aims Irritable bowel syndrome (IBS) is associated with exaggerated cerebral response including emotional processing following visceral stimulation; though data on this issue is available in female IBS patients, it is scanty among males. Hence, we aimed to study brain response of male IBS patients following rectal balloon distension as compared to healthy controls using functional magnetic resonance imaging (fMRI). Data between diarrhea and constipation predominant IBS (IBS-D and IBS-C) were also compared. Methods Rectal balloon distension threshold was assessed in 20 male IBS patients (10 IBS-C and 10 IBS-D) and 10 age-matched male healthy controls. Subsequently, fMRI on all the participants was performed at their respective rectal pain threshold. The fMRI data were analysed using the Statistical Parametric Mapping software. Results IBS patients showed greater cerebral activations in insula, middle temporal gyrus, and cerebellum in the left hemisphere compared to healthy controls. Neural activation was found in bilateral precuneus/superior parietal lobules in controls but not in patients with IBS. The brain activation differed among IBS-C and IBS-D patients; while the right mid-cingulate cortex was activated in IBS-C, the left inferior orbito-frontal cortex, left calcarine, and bilateral fusiform gyri were activated among patients with IBS-D following rectal balloon distension. Conclusions Brain response to rectal balloon distension differed among male patients with IBS and controls and among patients with IBS-C and IBS-D. Differential activation among patients with IBS-C and IBS-D was seen in the brain regions controlling affective motivation, homeostatic emotions, and autonomic responses to pain. (J Neurogastroenterol Motil 2017;23:415-427)

DTMF Based Irrigation Water Pump Control System

Mandeep Guleria,Akanksha Rathi,Deepraj Singh Sajwan,Jasbir Singh Negi,Nidhi Chauhan 사단법인 미래융합기술연구학회 2017 아시아태평양융합연구교류논문지 Vol.3 No.3

As a result of advancement in technology it became easy for human beings to ease their work and at the same time complete the workwith less human force, less time and with better results. In this project we look forward to telephonic signaling approach using Dual tone multi-frequency technique in controlling the various electrical loads such as irrigation water pumps situated in inaccessible areas. The present work is based on the principle of Dual tone multi-frequency (DTMF) signal received from any mobile phone to switch on-off the desired electrical loads such as in our farms, in factories, tube wells, irrigation water pumps etc. In our agricultural fields the tube wells are spread away from one another and thus operating them becomes very hectic as the person has to run from one place to another to operate the loads. Similar thing happens in our households also. Keeping this thing in mind a system has been designed so that it can ease the farmer as well as save time using the DTMF technology to control the loads remotely.

Study of Flexural Strength and Leachate Analysis of Fly Ash-Lime-Gypsum Composite Mixed with Treated Tire Chips

S. P. Guleria,R. K. Dutta 대한토목학회 2013 KSCE Journal of Civil Engineering Vol.17 No.4

The paper presents the study of flexural strength of reference mix containing fly ash +8% lime +0.9% gypsum mixed with treated tire chips along with leachate analysis. The tire chips used in this investigation were treated with water, sodium hydroxide and carbon tetra chloride. The tire chip content was varied from 5% to 15%, curing period from 7 to 180 days and cured by two different methods (in dessicator and water-filled container) of curing. The results of this study revealed that flexural strength of the reference mix mixed with treated tire chips was higher in comparison to the reference mix with dry tire chips. Flexural strength increased with the increase in curing period and change in curing method from dessicator to water-filled container. Results further reveal that flexural strength decreases with the increase in the tire chip content. Further inclusion of tire chips in the reference mix changes the cracking pattern and deflection. The results of leachate analysis reveal that the reference mix with/without treated tire chips has shown a reduction in the leachablity of sodium and zinc in comparison to pure tire chips and the reference mix, respectively. The leachablity of calcium, sulphate, manganese in comparison to pure tire chips was higher. The composite made from reference mix mixed with treated tire chips has shown a reduction in the leaching of iron in comparison to the reference mix. A low level of leaching of manganese was observed from the reference mix with and without dry/treated tire chips. Further, no evidence of presence of harmful metals like copper, lead, chromium, cadmium and nickel were found in the leachate of the composite.

Association of the -2518 A/G Polymorphism of MCP-1 with Breast Cancer in Punjab, North-West India

Sambyal, Vasudha,Guleria, Kamlesh,Kapahi, Ruhi,Manjari, Mridu,Sudan, Meena,Uppal, Manjit Singh,Singh, Neeti Rajan Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.16

Background: Monocyte chemoattractant protein-1 (MCP-1) is a major chemokine thought to be responsible for monocyte and T-lymphocyte recruitment in acute inflammatory conditions and recruitment of macrophages in tumors. It is also implicated in cardiovascular disease, rheumatoid arthritis and chronic obstructive pulmonary disease. The aim of the present study was to investigate the correlation between MCP-1 -2518 A/G polymorphism and breast cancer risk in patients from Amritsar city of Punjab state in North-West India. Materials and Methods: We screened DNA samples of 200 sporadic breast cancer patients and 200 age and gender matched unrelated healthy individuals for MCP-1 -2518 A/G polymorphism using the PCR-RFLP method. Results: A significantly increased frequency of the GG genotype was observed in patients as compared to controls. Individuals carrying the MCP1 -2518GG genotype had a two fold risk for breast cancer (OR=2.06, 95%CI, 1.06-3.98; p=0.03). Genetic models analysis revealed a significant association between MCP-1 -2518 A/G polymorphism and cancer risk in homozygous co-dominant (OR=2.06, 95%CI, 1.06-3.98; p=0.03) and recessive (OR=1.97, 95%CI, 1.05-3.70; p=0.03) models. Conclusions: We conclude that the GG genotype of the MCP-1-2518 A/G polymorphism is associated with increased risk to breast cancer in Punjab, North-West India.

Analysis of TP53 Polymorphisms in North Indian Sporadic Esophageal Cancer Patients

Kaur, Sukhpreet,Sambyal, Vasudha,Guleria, Kamlesh,Manjari, Mridu,Sudan, Meena,Uppal, Manjit Singh,Singh, Neeti Rajan,Singh, Gursimran,Singh, Harpreet Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.19

Background: To investigate the relationship of five TP53 polymorphisms (p.P47S, p.R72P, PIN3 ins16bp, p.R213R and r.13494g>a) with the esophageal cancer (EC) risk in North Indians. Materials and Methods: Genotyping of p.P47S, p.R72P, PIN3 ins16bp, p.R213R and r.13494g>a polymorphisms of TP53 in 136 sporadic EC patients and 136 controls using polymerase chain reaction and PCR-RFLP. Results: The frequencies of genotype RR, RP and PP of p.R72P polymorphism were 16.91 vs 26.47%, 58.82 vs 49.27% and 24.27 vs 24.27% among patients and controls respectively. We observed significantly increased frequency of RP genotype in cases as compared to controls (OR=1.87, 95% CI, 1.01-3.46, p=0.05). The frequencies of genotype A1A1, A1A2 and A2A2 of PIN3 ins16bp polymorphism were 69.12 vs 70.59%, 27.20 vs 25% and 3.68 vs 4.41% among patients and controls. There was no significant difference among genotype and allele distribution between patients and controls. The frequencies of genotype GG, GA and AA of r.13494g>a polymorphism were 62.50 vs 64.70%, 34.56 vs 30.15% and 2.94 vs 5.15% among patients and controls respectively. No significant difference between genotype and allele frequency was observed in the patients and controls. For p.P47S and p.R213R polymorphisms, all the cases and controls had homozygous wild type genotype. The RP-A1A1-GG genotype combination shows significant risk for EC (OR=2.01, 95%CI: 1.01-3.99, p=0.05). Conclusions: Among the five TP53 polymorphisms investigated, only p.R72P polymorphism may contributes to EC susceptibility.

TP53 Polymorphisms in Sporadic North Indian Breast Cancer Patients

Sharma, Sarika,Sambyal, Vasudha,Guleria, Kamlesh,Manjari, Mridu,Sudan, Meena,Uppal, Manjit Singh,Singh, Neeti Rajan,Bansal, Darpan,Gupta, Arun Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.16

Background: The purpose of this study was to evaluate the potential association of five (p.P47S, p.R72P, PIN3 Ins16bp, p.R213R and r.13494g>a) polymorphisms of TP53 with the risk of developing breast cancer in North Indian Punjabi population. Methods: We screened DNA samples of 200 sporadic breast cancer patients (197 females and 3 males) and 200 unrelated healthy, gender and age matched individuals for the polymorphisms. Results: For the p.P47S polymorphism, we observed the PP genotype in 99.5% of the patients and PS genotype in only 1 patient. All the controls had the wild type PP genotype. The frequency of RR, RP and PP genotype of p.R72P was 23.5% vs 33.5%, 51.5% vs 45.5% and 25% vs 21% in patients and controls respectively. Heterozygous (RP) genotype was increased in breast cancer patients as compared to controls (51.5 vs 45.5%) and showed 1.61 fold significantly increased risk for breast cancer (OR=1.61, 95% CI, 1.01-2.58, p=0.04). In breast cancer patients the frequencies of A1A1, A1A2 and A2A2 genotypes of PIN3 Ins16bp polymorphism were 67%, 26% and 7% respectively whereas in controls the genotype frequencies were 68.5%, 27.5% and 4% respectively, with no significant difference. For p.R213R (c.639A>G), all individuals had homozygous wild type genotype. The frequencies of GG, GA and AA genotypes of TP53 r.13494g>a polymorphism were 62 vs 67.5%, 33 vs 28% and 5 vs 4.5% in patients and controls respectively, again without significant difference. We observed that RP-A1A1 genotype combination of p.R72P and PIN3 Ins16bp and RP-GG combination of p.R72P and r.13494g>a polymorphism showed significant risk of breast cancer (OR=1.65, 95%CI: 0.98-2.78, p=0.05; OR=1.72, 95%CI: 1.01-2.92, p=0.04). Conclusion: The results of present study indicated that among the five TP53 polymorphisms investigated, the p.R72P polymorphism, and the RP-A1A1 and RP-GG genotype combination contribute to breast cancer susceptibility in North Indians.