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Enhanced Production and Partial Purification of Glucoamylase from Mutated Bacillus sp. FME
Gudi Satheesh Kumar,Muni Ramanna Gari Subhosh Chandra,Yakasiri Nagasai Sujana,Bontha Rajasekhar Reddy,Yong Lark Choi 한국응용생명화학회 2009 Applied Biological Chemistry (Appl Biol Chem) Vol.52 No.5
In this study, a potent newly-isolated glucoamylase producing actively growing cells of Bacillus sp.FME was subjected to UV irradiation and ethidium bromide (EtBr) mutagenesis. The promising colonies were further screened for glucoamylase production via plate assays and submerged enzyme production at the flask level. Amongst all of the tested colonies, the best mutant, Bacillus sp. FME 2, selected from UV irradiation (20 min) and EtBr (1 mg/mL), was shown to be the most promising. The yield of glucoamylase generated by the mutant strain was approximately 3.0 fold and 1397 U/mL with an incubation period of 24 h, which was larger than the yield generated by the wild-type strain. The glucoamylase was partially purified using ammonium sulphate precipitation followed by gel exclusion chromatography. The enzyme was partially purified 3.0-fold to homogeneity with a final recovery of 66% and a specific activity of 1145 U/mg protein for the mutant strain. The molecular mass was approximately 67.1 kDa, as determined by SDS-PAGE. The active band was observed as a clear colorless area on zymogram analysis, which indicated an absence of glucoamylase isoforms. The results of thin-layer chromatography identified this enzyme as a glucoamylase.
Enhanced Production and Partial Purification of Glucoamylase from Mutated Bacillus sp. FME
( Gudi Satheesh Kumar ),( Muni Ramanna Gari Subhosh Chandra ),( Yakasiri Nagasai Sujana ),( Bontha Rajasekhar Reddy ),( Yong Lark Choi ) 한국응용생명화학회 2009 Applied Biological Chemistry (Appl Biol Chem) Vol.52 No.5
Enhanced Production and Partial Purification of Glucoamylase from Mutated Bacillus sp. FME
Kumar, Gudi Satheesh,Chandra, Muni Ramanna Gari Subhosh,Sujana, Yakasiri Nagasai,Reddy, Bontha Rajasekhar,Choi, Yong-Lark The Korean Society for Applied Biological Chemistr 2009 Applied Biological Chemistry (Appl Biol Chem) Vol.52 No.5
In this study, a potent newly-isolated glucoamylase producing actively growing cells of Bacillus sp. FME was subjected to UV irradiation and ethidium bromide (EtBr) mutagenesis. The promising colonies were further screened for glucoamylase production via plate assays and submerged enzyme production at the flask level. Amongst all of the tested colonies, the best mutant, Bacillus sp. FME 2, selected from UV irradiation (20 min) and EtBr (1 mg/mL), was shown to be the most promising. The yield of glucoamylase generated by the mutant strain was approximately 3.0 fold and 1397 U/mL with an incubation period of 24 h, which was larger than the yield generated by the wild-type strain. The glucoamylase was partially purified using ammonium sulphate precipitation followed by gel exclusion chromatography. The enzyme was partially purified 3.0-fold to homogeneity with a final recovery of 66% and a specific activity of 1145 U/mg protein for the mutant strain. The molecular mass was approximately 67.1 kDa, as determined by SDS-PAGE. The active band was observed as a clear colorless area on zymogram analysis, which indicated an absence of glucoamylase isoforms. The results of thin-layer chromatography identified this enzyme as a glucoamylase.
Gudi, Satheesh Kumar,Gurramkonda, Chandrasekhar,Rather, Gulam,Chandra, Muniramanna Gari Subohsh,Mangamuri, Usha Kiranmayi,Podha, Shdhakar,Choi, Yong-Lark 한국응용생명화학회 2013 Applied Biological Chemistry (Appl Biol Chem) Vol.56 No.4
Glucoamylase (EC 3.2.1.3) is an important group of enzymes in starch processing, also referred to as amyloglucosidases, which are exo-acting amylases that release glucose from the non-reducing end of starch and related oligosaccharides. The glucoamylase newly isolated from the Aspergillus niger FME) was reported for the first time. This enzyme was produced by detergent-mediated release and purified to ~9.11 fold using Sephadex-G 100 and ion-exchange chromatography. Molecular mass of the glucoamylase was ~36 kDa as determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The product of starch hydrolysis, analysed by thin-layer chromatography, showed the presence of glucose. The optimum pH and temperature for glucoamylase activity was 5.0 and $45^{\circ}C$, respectively. The $K_m$ and $V_{max}$ values of the enzyme were also determined using soluble starch as substrate as $94{\mu}g/mL$ and 39.02 U/mg, respectively. Moreover, glucoamylase was slightly activated by presence of Na and K ions and 10-20% inhibition was observed in presence of $Zn^{2+}$, $Sn^{2+}$, $Mg^{2+}$, $Ni^{2+}$, $Mn^{2+}$, and almost 80% with $Cu^{2+}$ ions, whereas the presence of ethylene diamine tetra acetic acid (EDTA) did not show significant inhibition. Glucoamylase, also assayed for surfactant property, shows significant surfactant tolerance at high concentrations of detergent and can retain 90% of its activity. Finally, secondary structure analysis of glucoamylase by circular dichroism spectroscopy showed the presence of 48% ${\alpha}$-helix, 11% ${\beta}$-sheet, and 41% random structure.
Satheesh Kumar Gudi,최용락,Chandrasekhar Gurramkonda,Gulam Rather,Muniramanna Gari Subohsh Chandra,Usha Kiranmayi Mangamuri,Shdhakar Podha 한국응용생명화학회 2013 Applied Biological Chemistry (Appl Biol Chem) Vol.56 No.4
Glucoamylase (EC 3.2.1.3) is an important group of enzymes in starch processing, also referred to as amyloglucosidases,which are exo-acting amylases that release glucose from the nonreducing end of starch and related oligosaccharides. The glucoamylase newly isolated from the Aspergillus niger FME)was reported for the first time. This enzyme was produced by detergent-mediated release and purified to ~9.11 fold using Sephadex-G 100 and ion-exchange chromatography. Molecular mass of the glucoamylase was ~36 kDa as determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The product of starch hydrolysis, analysed by thin-layer chromatography, showed the presence of glucose. The optimum pH and temperature for glucoamylase activity was 5.0 and 45oC,respectively. The Km and Vmax values of the enzyme were also determined using soluble starch as substrate as 94 μg/mL and 39.02 U/mg, respectively. Moreover, glucoamylase was slightly activated by presence of Na and K ions and 10–20% inhibition was observed in presence of Zn2+, Sn2+, Mg2+, Ni2+, Mn2+, and almost 80% with Cu2+ ions, whereas the presence of ethylene diamine tetra acetic acid (EDTA) did not show significant inhibition. Glucoamylase, also assayed for surfactant property, shows significant surfactant tolerance at high concentrations of detergent and can retain 90% of its activity. Finally, secondary structure analysis of glucoamylase by circular dichroism spectroscopy showed the presence of 48% α-helix, 11% β-sheet, and 41%random structure.