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RISS 인기검색어
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- 저자 : Gongcheng Wang (검색결과 4 건)
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Chemical Composition, Antioxidant and Cytoprotective Activities of Lotus Receptacle
Weicheng Hu,Gongcheng Wang,Ting Shen,Yuning Wang,Boran Hu,Xinfeng Wang,Lei Wu,Pengxia Li,Lilian Ji 한국원예학회 2015 Horticulture, Environment, and Biotechnology Vol.56 No.5
The nutritional composition (ash, protein, fat, crude fiber, and carbohydrate) and antioxidant activity of lotus receptacles (LRs) were studied. The LRs contained 88.25% carbohydrates, 22.90% crude fiber, 5.79% crude ash, 0.77% crude protein content, and 5.19% crude lipid on a dry weight basis. The ethanolic extract of lotus receptacle (ELR) exerted strong scavenging effects on 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical, hydroxyl radical (•OH), superoxide anion (O2-), and hydrogen peroxide (H2O2), as well as high reducing power. ELR pretreatment effectively elevated cell viability and decreased malondialdehyde (MDA) levels in H2O2-treated RAW 264.7 cells. Additionally, ELR had high phenolic and flavonoid contents with values of 318.15 mg gallic acid equivalent/g and 239.06 mg rutin equivalent/g, respectively. Moreover, the bioactivity-guided study of ELR resulted in the isolation and identification of quercetin-3-O-β-D-glucopyranoside (QOG), a dietary flavonoid. QOG exhibited strong DPPH free radical scavenging activity, with an IC50 value of 24.26 μM; docking studies revealed that QOC formed one hydrogen bond with LYS263 in the active site, suggesting it is, in part, responsible for the free activity of LR.
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Li, Fu,Cao, Yufeng,Luo, Yanyan,Liu, Tingwu,Yan, Guilong,Chen, Liang,Ji, Lilian,Wang, Lun,Chen, Bin,Yaseen, Aftab,Khan, Ashfaq A.,Zhang, Guolin,Jiang, Yunyao,Liu, Jianxun,Wang, Gongcheng,Wang, Ming-Kui The Korean Society of Ginseng 2019 Journal of Ginseng Research Vol.43 No.4
Background: The leaves and roots of Panax ginseng are rich in ginsenosides. However, the chemical compositions of the leaves and roots of P. ginseng differ, resulting in different medicinal functions. In recent years, the aerial parts of members of the Panax genus have received great attention from natural product chemists as producers of bioactive ginsenosides. The aim of this study was the isolation and structural elucidation of novel, minor ginsenosides in the leaves of P. ginseng and evaluation of their antiinflammatory activity in vitro. Methods: Various chromatographic techniques were applied to obtain pure individual compounds, and their structures were determined by nuclear magnetic resonance and high-resolution mass spectrometry, as well as chemical methods. The antiinflammatory effect of the new compounds was evaluated on lipopolysaccharide-stimulated RAW 264.7 cells. Results and conclusions: Two novel, minor triterpenoid saponins, ginsenoside $LS_1$ (1) and 5,6-didehydroginsenoside $Rg_3$ (2), were isolated from the leaves of P. ginseng. The isolated compounds 1 and 2 were assayed for their inhibitory effect on nitric oxide production in LPS-stimulated RAW 264.7 cells, and Compound 2 showed a significant inhibitory effect with $IC_{50}$ of $37.38{\mu}M$ compared with that of NG-monomethyl-L-arginine ($IC_{50}=90.76{\mu}M$). Moreover, Compound 2 significantly decreased secretion of cytokines such as prostaglandin $E_2$ and tumor necrosis factor-${\alpha}$. In addition, Compound 2 significantly suppressed protein expression of inducible nitric oxide synthase and cyclooxygenase-2. These results suggested that Compound 2 could be used as a valuable candidate for medicinal use or functional food, and the mechanism is warranted for further exploration.
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Fuli Li,Yufeng Cao,Tingwu Liu,Guilong Yan,Liang Chen,Lilian Ji,Lun Wang,Bin Chen,Aftab Yaseen,Ashfaq A. Khan,Guo-Lin Zhang,Yunyao Jiang,Jianxun Liu,Gongcheng Wang,Ming-Kui Wang,Weicheng Hu 고려인삼학회 2019 Journal of Ginseng Research Vol.43 No.4
Background: The leaves and roots of Panax ginseng are rich in ginsenosides. However, the chemical compositionsof the leaves and roots of P. ginseng differ, resulting in different medicinal functions. In recent years,the aerial parts of members of the Panax genus have received great attention fromnatural product chemistsas producers of bioactive ginsenosides. The aim of this study was the isolation and structural elucidation ofnovel, minor ginsenosides in the leaves of P. ginseng and evaluation of their antiinflammatory activity in vitro. Methods: Various chromatographic techniques were applied to obtain pure individual compounds, andtheir structures were determined by nuclear magnetic resonance and high-resolution mass spectrometry,as well as chemical methods. The antiinflammatory effect of the new compounds was evaluated onlipopolysaccharide-stimulated RAW 264.7 cells. Results and conclusions: Two novel, minor triterpenoid saponins, ginsenoside LS1 (1) and 5,6-didehydroginsenoside Rg3 (2), were isolated from the leaves of P. ginseng. The isolated compounds 1and 2 were assayed for their inhibitory effect on nitric oxide production in LPS-stimulated RAW 264.7cells, and Compound 2 showed a significant inhibitory effect with IC50 of 37.38 mM compared with that ofNG-monomethyl-L-arginine (IC50 ¼ 90.76 mM). Moreover, Compound 2 significantly decreased secretionof cytokines such as prostaglandin E2 and tumor necrosis factor-a. In addition, Compound 2 significantlysuppressed protein expression of inducible nitric oxide synthase and cyclooxygenase-2. These resultssuggested that Compound 2 could be used as a valuable candidate for medicinal use or functional food,and the mechanism is warranted for further exploration.
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Lei Wu,Gongcheng Wang,Ting Shen,Long You,Weicheng Hu,Chuan-Ling Si 한국원예학회 2017 Horticulture, Environment, and Biotechnology Vol.58 No.3
Mahonia bealei (Fort.) Carr., is an economic plant cultivated in Southwest China commonly used in traditionalChinese medicine. In this study, a response surface methodology was used to optimize experimental conditions forthe extraction of phenolic compounds from the leaves of Mahonia bealei (Fort.) Carr. The highest extraction ratio ofphenolic compounds yielded 39.1 mg gallic acid equivalent/g of dry weight using an ethanol concentration of 30%(v/v) as a solvent and a liquid-to-solid ratio of 21:1 (mL·g-1) for 2 h in 63°C. The crude material was extractedunder optimal conditions, enriched, and then purified through a D-101 macroporous adsorption resin, giving a phenoliccompound-enriched fraction we named TPMB. Evaluation of its in vitro antioxidant activity suggested that TPMBsignificantly scavenged the 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical, superoxide radical, and hydrogen peroxidein a concentration-dependent manner. In addition, TPMB also exhibited a strong reducing ability and provided protectionagainst oxidative damage induced by oxidative stress in cellular antioxidant activity assays. The results from this studyindicate the suitability of the response surface methodology in optimizing the solvent extraction of phenolic compoundsfrom M. bealei. Further research showed that TPMB possesses a strong anti-radical activity and may be an effectiveoxidation resistance treatment in the medical and food industries.
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