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WonJin Kim(김원진),JiUn Lee(이지운),Gi-Hoon Yang(양지훈),Hyeongjin Lee(이형진),YongBok Kim(김용복),Minseong Kim(김민성),YoungWon Koo(구영원),GeunHyung Kim(김근형) 대한기계학회 2016 대한기계학회 춘추학술대회 Vol.2016 No.12
Collagen- based cell-printing technology has provided a new strategy for tissue engineering. However, although collagen-based scaffolds can provide an outstanding biofunctional benefits, their low mechanical strength and poor controllability have been limitation for their usage as hard tissue regeneration. To overcome this limitation, α-tricalcium phosphate (α-TCP) has been used for biomedical scaffolds. α-TCP is biocompatible and soluble material, and hydrolyzed rapidly to calcium-deficient hydroxyapatite which makes α-TCP a useful material for bone tissue regeneration. in this study, we fabricate 3-dimensional (3D) scaffold based on α-TCP/collagen struts coated with collagen-based bioink. To compare the physical and cellular activities, we used a scaffold composed of α-TCP/collagen scaffold coated with cell-embedded collagen. Following fabrication of the cell (MC3T3-E1)-embedded a-TCP/collagen scaffold, the cellular activities were evaluated in vitro.
골 조직 재생을 위한 전기수력학적 공정을 이용한 섬유구조의 PCL/ceramic 세포담체 제작
김민성(Minseong Kim),이형진(Hyeongjin Lee),전호준(HoJun Jeon),여명구(MyungGu Yeo),김용복(YongBok Kim),양지훈(Gi-Hoon Yang),김원진(WonJin Kim),김근형(GeunHyung Kim) 대한기계학회 2016 대한기계학회 춘추학술대회 Vol.2016 No.12
In this study, we fabricated a new ceramic fibrous scaffold, using the initial jet of an electrospinning process and ethanol media as a target. The fabricated three-dimensional (3D) fibrous ceramic structure was configured with multilayered micro-sized struts consisting of randomly entangled micro/nanofibrous architecture, similar to that of native extracellular matrixes (ECMs). The fabrication of the ceramic structure was highly dependent on various processing parameters, such as the surface tension of the target media, and the flow rate and weight fraction of the polymer solution. As a tissue regenerative material, the 3D fibrous ceramic scaffold was cultured with pre-osteoblasts to observe the initial cellular activities in comparison with a solid freeform fabricated 3D scaffold sharing a similar structural geometry. The micro/nanofibrous 3D fibrous ceramic strut scaffold exhibited significantly high initial cell attachment, proliferation, and viability compared to solid freeform fabricated 3D scaffold.
김예슬(YeSeul Kim),김민성(Minseong Kim),전호준(Hojun Jeon),김용복(YongBok Kim),이형진(Hyeongjin Lee),황헌(Heon Hwang),조재열(JaeYoul Cho),김근형(GeunHyung Kim) 대한기계학회 2013 대한기계학회 춘추학술대회 Vol.2013 No.12
The inherent properties of nanosized silica (sil), such as high biocompatibility, chemical and colloidal stability, and easy surface modification, have provided silica materials with a tremendous potential in biomedical applications. In this study, the biocomposites consisting of poly (ε-caprolactone) (PCL) and Sil fabricated by a melt-plotting/coating process can be applied as a potential scaffold for bone tissue regeneration. The pore size and strut diameter of the multi-layered biocomposites were fixed at approximately 300㎛ and 300㎛, respectively, and the morphology, hydrophilic properties, water-absorption, and mechanical strength of various compositions (1.8, 4.8, 9.4wt% of sil) in the composites were evaluated. Through the water-contact angle and water-absorption, the bio-composites displayed dramatically increased hydrophilic properties, and highly roughened surface compared to the pure PCL scaffold. The in vitro biocompatibilities (cell proliferation and mineralization) of the bio-composites wereexamined using pre-osteoblasts (MC3T3E1). Based on scanning electron microscope images, the cells were more easily adhered and grown on the surface of the bio-composites, showing enhanced mineral deposition compared to the pure PCL scaffold after 14 days of cell culture. These results were because the coated sil component in the bio-composites could induce the osteogensis of the composites. Based on the physical and biological activities, we believe that the biocomposite will be a potential biomaterial for enhancing bone tissue regeneration.
Fabrication of a biocomposite reinforced with hydrophilic eggshell proteins
Kim, GeunHyung,Min, Taijin,Park, Su A,Kim, Wan Doo,Koh, Young Ho Institute of Physics Publishing Ltd 2007 Biomedical Materials Vol.2 No.4
<P>Soluble eggshell proteins were used as a reinforcing material of electrospun micro/nanofibers for tissue engineering. A biocomposite composed of poly(ϵ-caprolactone) (PCL) micro/nanofibers and soluble eggshell protein was fabricated with a two-step fabrication method, which is an electrospinning process followed by an air-spraying process. To achieve a stable electrospinning process, we used an auxiliary cylindrical electrode connected with a spinning nozzle. PCL biocomposite was characterized in water contact angle and mechanical properties as well as cell proliferation for its application as a tissue engineering material. It showed an improved hydrophilic characteristic compared with that of a micro/nanofiber web generated from a pure PCL solution using a typical electrospinning process. Moreover, the fabricated biocomposite had good mechanical properties compared to a typical electrospun micro/nanofiber mat. The fabricated biocomposite made human dermal fibroblasts grow better than pure PCL. From the results, the reinforced polymeric micro/nanofiber scaffold can be easily achieved with these modified processes.</P>
Highly porous 3D nanofiber scaffold using an electrospinning technique
Kim, GeunHyung,Kim, WanDoo Wiley Subscription Services, Inc., A Wiley Company 2007 Journal of Biomedical Materials Research Part B Vol. No.
<P>A successful 3D tissue-engineering scaffold must have a highly porous structure and good mechanical stability. High porosity and optimally designed pore size provide structural space for cell accommodation and migration and enable the exchange of nutrients between the scaffold and environment. Poly(ε-carprolactone) fibers were electrospun using an auxiliary electrode and chemical blowing agent (BA), and characterized according to porosity, pore size, and their mechanical properties. We also investigated the effect of the BA on the electrospinning processability. The growth characteristic of human dermal fibroblasts cells cultured in the webs showed the good adhesion with the blown web relative to a normal electrospun mat. The blown nanofiber web had good tensile properties and high porosity compared to a typical electrospun nanofiber scaffold. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2006</P>
Injectable hierarchical micro/nanofibrous collagen-based scaffolds
Kim, Minseong,Choe, YoungEun,Kim, GeunHyung Elsevier 2019 Chemical Engineering Journal Vol. No.
<P><B>Abstract</B></P> <P>Collagen exhibits an exceptional biocompatible performance that can be used in tissue regeneration applications. Therefore, it is widely used in various structural shapes, e.g., nanofibers and microscale struts in multi-layered structure. Here, we developed a unique hierarchical collagen structure consisting of spatially coiled microfibers and inter-laden nanofibers with collagen type-I by using a modified electrohydrodynamic-printing process. The hierarchical collagen scaffolds showed not only an outstanding mechanical flexibility and creep recovery, demonstrating good injectable property, but also excellent cellular activities compared to those of the controls (an electrospun collagen nanofibrous mat and a three-dimensionally printed collagen scaffold). To demonstrate its feasibility as an injectable scaffold, it was injected and deployed through micro-sized needles. An exceptional injectability was observed without any breakage of the initial pore geometry. Here, the bone marrow-derived mesenchymal stem cells were cultured on the collagen scaffold for 1 and 3 days. According to several cellular activities including cell-viability, the cultured cells were well protected for the injection process and exhibited excellent biocompatibilities as compared to those of the controls.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Hierarchical micro/nanofibrous collagen was fabricated for tissue regeneration. </LI> <LI> The fibrous collagen structure showed shape recoverable properties. </LI> <LI> The hierarchical collagen scaffolds exhibited significant biological activities. </LI> </UL> </P>