암환자의 삶의 질 도구개발

태영숙,강은실,이명화,박금자 성인간호학회 2000 성인간호학회지 Vol.12 No.4

This study was undertaken to develop an instrument to be used for measuring the concept of duality of life of Korean patients with cancer multidimensionary and correctly. It can contribute in holistic nursing care for Korean cancer patients and also provide and validate basic data to help oncology nurses measure the outcome of nursing intervention correctly. To develop this instrument, the researchers first estabilished a conceptual framework based on the results of qualitative data analysis and indepth interview method Development of the scale was conducted using a method in which 31 items were assessed by subjects' self report using linear analogue scales. The subjects were 79 D.M. Patients. 103 patients with acute illness, and 91 cancer Patients residing in Busan, Korea. Data were collected during the period from July, 24 to August 14. 2000. This instrument consisted of 31 items with a self report stale. This instrument covered 4 dimensions of cancer patients : 1) Physical wellbeing 2) psychological wellbeing 3) social wellbeing and 4)spiritual wellbeing. Each item had a possible score of 10. The reliability of the scale was tested with Cronbach's alpha. Validity was evaluated by examining the relationships of this scale. Youn's Quality of Life Questionnare scores and the Simple Quality of Life scale. Two separate runs of multiple regression were used to predict scores on the Simple Quality of Life measuremend. Further validation was obtained by examining the correlation between the instrument subscores and Youn's Quality of Life measurement subscore for convergence of this scale. Examination of the discriminant. power of the instrument was done using ANOVA test. The results are summarized as follows : 1.The reliability of the instrument for the quality of life was 0.8321 (Cronbach's alpha.), physical wellbeing dimension 0.6343. Psychological wellbeing dimension 0.6501, spiritual wellbeing dimension 0.5883. 2.This instrument had a high correlation with Youn's Quality of Life measurement(r= 0.636) in cancer Patients, whereas it had a low correlation with Simple Quality of Life measurement(r= 0.455) in cancel patients. In the D.M. patients, the instrument correlated with both the Youn's Quality of Llfe measurement and Simple Quality of life measurement(r=0.313. r= 0.407) and in the acute stage patients. the instrument had no correlation. 3.Multiple regression of individual Items on the Simple Quality of Life scores accounted for 56.8% of the variance in the Simple Quality of Life measurement, whereas, Youn's Quality of Life measurement scores accounts for 31.7%. The correlations collected from the three group had the same patterns of variations but especially the instrument developed in this study had higher disciminant power than that of Youn's Quality of Life Measurement.

Initial Risk Assessment of Acetanilide with Respect to Ecological Integrity

Lee, Su-Rae,Choi, Seon-Ju,Lee, Mi-Kyung,Nam, U-Kyung,Chung, Sun-Hwa,Seog, Geum-Su,Park, Kwang-Sik,Kim, Kyun,Kim, Yong-Hwa 한국환경독성학회 2000 환경독성보건학회지 Vol.15 No.1

아세트아닐리드는 의약품과 염료의 합성과정에서 중간체로서 공기와 폐수를 통하여 환경 중에 방출 될 수 있다. 아세트아닐리드는 호기적 조건하에서 신속히 생분해되고 OH래디컬의 존재하에 간접적으로 광분해된다. 생물농축계수는 4.5로 추정되므로 수생생물에서의 생물농축은 낮을 것으로 예상된다. 아세트아닐리드에 관한 생태독성학적 데이터 조사결과 4종 어류에 대한 급성독성치만 보고되어 있으며, EUSES시스템에 의하면 어류에서의 최저 PNEC값 (예상 무작용농도)은 0.01㎎/l이고 표면수에서의 PEC값(예상 환경농도)은 지역수준에서 최악의 경우 9.1?0^(-5)㎎/l이다. 지역수준에서 표면수에 대한 아세트아닐리드의 RCR(위해성지수)은 9.1?0^(-3)으로 추정되어 어류에 대한 안전성을 충분하다. 그러나 국지 수준에서의 RCR은 물과 침적물에서 각각 1.3과 1.6이므로 제조공장 주변에서는 생태독성 위험이 존재할 것으로 추정된다. 아세트아닐리드의 환경위해성 평가를 보다 정확하기 하기 위해서는 물벼룩과 조류에 대한 급성독성 자료가 보완되어야 할 것이며, 따라서 이에 대한 실험이 진행되어야 할 것으로 사료된다. Acetanilide may be released into the environment through air and wastewater from its production and use sites as an intermediate in the synthesis of phar-maceuticals and dyes. Acetanilide is biodegraded rapidly under aerobic conditions and decomposed by indirect photolysis in the presence of OH radicals. An estimated bioconcentration factor of 4.5 suggests that bioaccumulation in aquatic organisms is low. Ecotoxicological data on acetanilide exist on acute toxicity to fishes of 4 species only. According to the EUSES system, the lowest PNEC (Predicted no effect concentration) in fishes is 0.01 mg/l and PEC (Predicted environmental concentration) for surface water on a regional scale is 9.1?0^(-5) mg/l as the worst case. RCR (Risk characterization ratio) of acetanilide for surface water on a regional scale was estimated as 9.1?0^(-3), which is safe enough C(=)or fishes. RCR on a local basis slightly exceeds the value I in water and sediment; that is, 1.3 and 1.6, respectively, which suggests the existence of ecotoxicological risk at the vicinity of the manufacturing site. For the refinement of environmental risk assessment on acetanilide, more data should be collected regarding prolonged fish toxicity, acute toxicity to ward daphnia and algae. It is, therefore, recommended that acetanilide should be a candidate for further work to supplement the lacking data until it is proved to be safe in the ecotoxicological aspects.

Platycodi Radix and its active compounds ameliorate against house dust mite-induced allergic airway inflammation and ER stress and ROS by enhancing anti-oxidation

Lee, Hwa-Young,Lee, Geum-Hwa,Kim, Hye-Kyung,Chae, Han-Jung Elsevier 2019 Food and chemical toxicology Vol.123 No.-

<P><B>Abstract</B></P> <P>Allergic airway inflammation is an increasing global health problem, and novel strategies to prevent or ameliorate the condition are needed. The endoplasmic reticulum (ER) is involved in protein synthesis and maturation, and is a susceptible to sub-organelle stress including inflammation and ROS-amplifying signaling. Here, the effects of Platycodi Radix extracts (PRE) on house dust mite (HDM) extract (<I>Dematophagoides pteronyssius</I>)-induced asthma were investigated. Following 50, 100, or 200 mg/kg-PRE-treatment, the infiltration of inflammatory cells, ER stress, and NF-κB signaling were controlled. The expression of inflammatory cytokines and mucin5AC was also inhibited in the presence of PRE. Consistently, in the HDM-exposed human bronchial epithelial cells, ER stress and its associated ROS were significantly increased along with NF-κB signaling, which was also attenuated by PRE and its components. This study suggests that PRE might be useful as a therapeutic/preventive agent in HDM-associated allergic airway inflammation. ER stress and its associated ROS signaling involved in inflammation provide additional mechanistic insight into the underlying molecular mechanism.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Platycodi Radix inhibits HDM-induced allergic airway inflammation response in mice. </LI> <LI> Platycodi Radix protects HDM-induced nuclear translocation of NF-κB p65 and cytokine expression in mice. </LI> <LI> Platycodi Radix regulates HDM-induced ER stress, lipid peroxidation and ROS accumulation in mice. </LI> <LI> Platycoside E, platycodin D<SUB>3</SUB> and Platycodi Radix inhibits HDM-induced allergic airway inflammation response in mice. </LI> </UL> </P>

The Influence of Genotype Polymorphism on Morphine Analgesic Effect for Postoperative Pain in Children

Lee, Mi Geum,Kim, Hyun Jung,Lee, Keun Hwa,Choi, Yun Suk The Korean Pain Society 2016 The Korean Journal of Pain Vol.29 No.1

Background: Although opioids are the most commonly used medications to control postoperative pain in children, the analgesic effects could have a large inter-individual variability according to genotypes. The aim of this study was to investigate the association between single nucleotide polymorphisms and the analgesic effect of morphine for postoperative pain in children. Methods: A prospective study was conducted in 88 healthy children undergoing tonsillectomy, who received morphine during the operation. The postoperative pain score, frequency of rescue analgesics, and side effects of morphine were assessed in the post-anesthesia care unit. The children were genotyped for OPRM1 A118G, ABCB1 C3435T, and COMT Val158Met. Results: Children with at least one G allele for OPRM1 (AG/GG) had higher postoperative pain scores compared with those with the AA genotype at the time of discharge from the post-anesthesia care unit (P = 0.025). Other recovery profiles were not significantly different between the two groups. There was no significant relationship between genotypes and postoperative pain scores in analysis of ABCB1 and COMT polymorphisms. Conclusions: Genetic polymorphism at OPRM1 A118G, but not at ABCB1 C3435T and COMT Val158Met, influences the analgesic effect of morphine for immediate acute postoperative pain in children.

Bax Inhibitor 1 Increases Cell Adhesion through Actin Polymerization: Involvement of Calcium and Actin Binding

Lee, Geum-Hwa,Ahn, Taeho,Kim, Do-Sung,Park, Seoung Ju,Lee, Yong Chul,Yoo, Wan Hee,Jung, Sung Jun,Yang, Jae-Seong,Kim, Sanguk,Muhlrad, Andras,Seo, Young-Rok,Chae, Soo-Wan,Kim, Hyung-Ryong,Chae, Han-Jun American Society for Microbiology 2010 Molecular and cellular biology Vol.30 No.7

<B>ABSTRACT</B><P>Bax inhibitor 1 (BI-1), a transmembrane protein with Ca<SUP>2+</SUP> channel-like activity, has antiapoptotic and anticancer activities. Cells overexpressing BI-1 demonstrated increased cell adhesion. Using a proteomics tool, we found that BI-1 interacted with γ-actin via leucines 221 and 225 and could control actin polymerization and cell adhesion. Among BI-1<SUP>−/−</SUP> cells and cells transfected with BI-1 small interfering RNA (siRNA), levels of actin polymerization and cell adhesion were lower than those among BI-1<SUP>+/+</SUP> cells and cells transfected with nonspecific siRNA. BI-1 acts as a leaky Ca<SUP>2+</SUP> channel, but mutations of the actin binding sites (L221A, L225A, and L221A/L225A) did not change intra-endoplasmic reticulum Ca<SUP>2+</SUP>, although deleting the C-terminal motif (EKDKKKEKK) did. However, store-operated Ca<SUP>2+</SUP> entry (SOCE) is activated in cells expressing BI-1 but not in cells expressing actin binding site mutants, even those with the intact C-terminal motif. Consistently, actin polymerization and cell adhesion were inhibited among all the mutant cells. Compared to BI-1<SUP>+/+</SUP> cells, BI-1<SUP>−/−</SUP> cells inhibited SOCE, actin polymerization, and cell adhesion. Endogenous BI-1 knockdown cells showed a similar pattern. The C-terminal peptide of BI-1 (LMMLILAMNRKDKKKEKK) polymerized actin even after the deletion of four or six charged C-terminal residues. This indicates that the actin binding site containing L221 to D231 of BI-1 is responsible for actin interaction and that the C-terminal motif has only a supporting role. The intact C-terminal peptide also bundled actin and increased cell adhesion. The results of experiments with whole recombinant BI-1 reconstituted in membranes also coincide well with the results obtained with peptides. In summary, BI-1 increased actin polymerization and cell adhesion through Ca<SUP>2+</SUP> regulation and actin interaction.</P>

Ginger Extract Ameliorates Lipid Accumaulation and Endoplasmic Reticulum Stress of Adipose Tissues by SIRT-1-PGC-1α Signaling via AMPK Activation in ob/ob mice

Hwa-Jin Kim,Han-Jung Chae,Geum-Hwa Lee,Soon-Yeon Jeong,Seon-Ah Park,Hwa-Young Lee,The-Hiep Hoang,Young-Je Lim,Ji-Hyun Kim 한국식품영양과학회 2022 한국식품영양과학회 학술대회발표집 Vol.2022 No.10

Hepatoprotective and anti-ageing effects of EUL extract in aged rat

Geum-Hwa Lee,Hwa-Young Lee,Seon-Ah Park,Han-Jung Chae 한국식품영양과학회 2018 한국식품영양과학회 학술대회발표집 Vol.2018 No.10

D-Allulose ameliorates hyperglycemia through IRE1α sulfonation-RIDD- sirt1 decay axis in the skeletal muscle

Geum-Hwa Lee,Hwa-Young Lee,Han-Jung Chae 한국실험동물학회 2022 한국실험동물학회 학술발표대회 논문집 Vol.2022 No.7

4-phenylbutyric Acid Regulates Collagen Synthesis and Secretion Induced by High Concentrations of Glucose in Human Gingival Fibroblasts

Geum-Hwa Lee,Hyo-Won Oh,Hyun-Dae Lim,Wan Lee,Han-Jung Chae,Hyung-Ryong Kim 대한생리학회-대한약리학회 2011 The Korean Journal of Physiology & Pharmacology Vol.15 No.6

High glucose leads to physio/pathological alterations in diabetes patients. We investigated collagen production in human gingival cells that were cultured in high concentrations of glucose. Collagen synthesis and secretion were increased when the cells were exposed to high concentrations of glucose. We examined endoplasmic reticulum (ER) stress response because glucose metabolism is related to ER functional status. An ER stress response including the expression of glucose regulated protein 78 (GRP78), C/EBP homologous protein (CHOP), inositol requiring enzyme alpha (IRE-1Ձ) and phosphor- eukaryotic initiation factor alpha (p-eIF-2Ձ) was activated in the presence of high glucose. Activating transcription factor 4 (ATF-4), a downstream protein of p-eIF-2Ձ as well as a transcription factor for collagen, was also phosphorylated and translocalized into the nucleus. The chemical chaperone 4-PBA inhibited the ER stress response and ATF-4 phosphorylation as well as nuclear translocation. Our results suggest that high concentrations of glucose-induced collagen are linked to ER stress and the associated phosphorylation and nuclear translocation of ATF-4.

BI-1 enhances Fas-induced cell death through a Na+/H+-associated mechanism

( Geum Hwa Lee ),( Hyung Ryong Kim ),( Han Jung Chae ) 생화학분자생물학회(구 한국생화학분자생물학회) 2014 BMB Reports Vol.47 No.7

The role of Bax inhibitor-1 (BI-1) in the protective mechanism against apoptotic stimuli has been studied; however, as little is known about its role in death receptor-mediated cell death, this study was designed to investigate the effect of BI-1 on Fas-induced cell death, and the underlying mechanisms. HT1080 adenocarcinoma cells were cultured in high concentration of glucose media and transfected with vector alone (Neo cells) or BI-1-vector (BI-1 cells), and treated with Fas. In cell viability, apoptosis, and caspase-3 analyses, the BI-1 cells showed enhanced sensitivity to Fas. Fas significantly decreased cytosolic pH in BI-1 cells, compared with Neo cells, and this decrease correlated with BI-1 oligomerization, mitochondrial Ca2+ accumulation, and significant inhibition of sodium-hydrogen exchanger (NHE) activity. Compared with Neo cells, a single treatment of BI-1 cells with the NHE inhibitor EIPA or siRNA against NHE significantly increased cell death, which suggests that the viability of BI-1 cells is affected by the maintenance of intracellular pH homeostasis through NHE. [BMB Reports 2014; 47(7): 393-398]