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      • KCI등재

        Effect of water scarcity during thermal-humidity exposure on the mineral footprint of sheep

        GHASSEMINEJAD JALIL,이배훈,김지융,박규현,김원섭,성경일,이홍구 아세아·태평양축산학회 2020 Animal Bioscience Vol.33 No.12

        Objective: Combination of two stressors on alteration of mineral footprints in animals needs due attention to meet maximum production and welfare, particularly in grazing sheep. This study tested whether ewes (Ovis aries) exposed to water deprivation and thermal–humidity stressors had altered mineral footprints in their wool, serum, urine, and feces. Methods: Nine ewes (age = 3 years; mean body weight = 41±3.5 kg) were divided among a control group with free access to water, and treatment groups with water deprivation lasting either 2 h (2hWD) or 3 h (3hWD) after feeding. Using a 3×3 Latin square design, animals were assigned to treatment groups for three sampling periods of 21 days each (n = 9). Blood was collected by jugular venipuncture. Wool was collected at the end of periods 2 and 3. Metabolic crates designed with metal grated floors were used for urine and feces collection. We measured sodium (Na), magnesium (Mg), phosphorus (P), chloride (Cl), calcium (Ca), manganese (Mn), copper (Cu), iron (Fe), and zinc (Zn). Results: The wool mineral levels did not differ between the treatment groups, although K was marginally lower (p = 0.10) in the 2hWD group. The serum and urine mineral levels did not differ between the treatments (p>0.05). Fecal K was significantly lower in the 2hWD group than in the other groups (p≤0.05). Conclusion: In conclusion, water deprivation and thermal-humidity exposure altered the excretion of K, but not of other minerals, in the wool, urine, feces, or serum of ewes. Thus, no additional mineral supplementation is needed for water deprived ewes during thermal-humidity exposure.

      • KCI등재

        Effects of forage level and chromium-methionine chelate supplementation on performance, carcass characteristics and blood metabolites in Korean native (Hanwoo) steers

        성경일,GHASSEMINEJAD JALIL,홍석만,오상집,이배훈,PENG JINGLUN,지도현,김병완 한국축산학회 2015 한국축산학회지 Vol.57 No.3

        A feeding trial was carried out to determine the effects of chromium methionine (Cr-Met) chelate and forage levelover two years, 1st fattening and 2nd fattening period on growth parameters, carcass characteristics and bloodmetabolites of 46 Korean native (Hanwoo, Bos Taurus, BW = 183 ± 44 kg) steers. Treatments were: 1) Steers in thelow forage (LF) group were fed diets that consisted of 60% concentrate and 40% forage; 2) Steers in the highforage (HF) group were fed diets that consisted of 40% concentrate and 60% forage. Following the 1st fatteningperiod, steers (BW = 480 ± 37.6 kg) were randomly assigned to four treatment groups: LF (40 F plus no Cr-Metsupplementation in the 2nd fattening period), LFCM (40LF plus added 400 ppb of Cr-Met during the 2nd fatteningperiod), HF (60 F plus no added Cr-Met during the 2nd fattening period) and HFCM (60 F plus added 400 ppb ofCr-Met in the 2nd fattening period). Dry matter intake of the treatment diets did not differ during the raising and1th fattening period (P > 0.05). The ADG in the raising period showed no difference between the 40 F and 60 Fgroups (P > 0.05). Carcass characteristics including rib-eye area and meat yield index were higher in HF than theother treatment groups (P < 0.05). The HF group tended to show a higher (P = 0.08) marbling score than the LFgroup whereas the HFCM group showed a higher marbling score than the LFCM group (P < 0.05). HDL washigher and LDL lower in groups fed with Cr-Met than in other groups whereas glucose showed the lowest valuein HF group (P < 0.05). Triglyceride (TG), Cholesterol, PUN and total protein (TP) were the same among all treatmentgroups (P > 0.05). The Insulin concentration in the blood was significantly higher for the HFCM group than for the LF,LFCM and HF groups (P < 0.05). It is concluded that supplementation of chromium-methionine chelate could improvemeat quality in beef steers.

      • KCI우수등재

        Effects of feather processing methods on quantity of extracted corticosterone in broiler chickens

        Mohammad Ataallahi,GHASSEMINEJAD JALIL,Jun-Ik Song,Jin-Soo Kim,Kyu Hyun Park 한국축산학회 2020 한국축산학회지 Vol.62 No.6

        Corticosterone is known as a biological stress index in many species including birds. Feather corticosterone concentration (FCC) has increasingly been used as a measure for chronic stress status in broiler chickens. As sample preparation is the first step of analytical process, different techniques of feather matrix disruption need to be validated for obtaining better result in analysing corticosterone extraction. The current study was a validation of pulverizing the feather by bead beater (BB) and surgical scissors (SS) processing prior to corticosterone extraction in feather of broiler chickens. The type of feather processing prior to the hormone extraction may alter the final output. Thereby, finding a standard method according to laboratory facilities is pivotal. This study carried out to determine the effects of feather pulverization methods on the extraction amount of corticosterone in broiler chickens. Feathers were sampled from four weeks old Ross 308 broiler chickens (n = 12 birds). All broiler chickens were kept under the same environmental condition and had access to feed and water. Feather samples were assigned to one of the following processing methods 1) using a BB for pulverizing and 2) using a SS for chopping into tiny pieces. Each sample was duplicated into two wells during enzyme immunoassay (EIA) analysis to improve the accuracy of the obtained data. The results showed lower standard errors and constant output of FCC by using the BB method compared with the SS method. Overall comparison of FCC showed a significantly higher (p < 0.001) amount of the FCC in the BB compared with the SS. Overall, using the BB method is recommended over the SS method for feather processing due to the ability to homogenize a large number of samples simultaneously, ease of use and greater extraction of feather corticosterone.

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