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      • KCI등재

        Insect Resistance and Horticultural Trait Genetic Values of Potato Families

        Souza, Velci Queiroz de,Pereira, Arione da Silva,Silva, Giovani Olegario da,Carvalho, Fernando Iraja Felix de,Oliveira, Antonio Costa de The Korean Society of Crop Science 2008 Journal of crop science and biotechnology Vol.11 No.1

        This work aimed to evaluate 11 potato families for insect resistance horticultural traits. The families were derived from crosses between introduced insect resistant and adapted genotypes. A randomized complete block design, with three replications was used. The plot consisted of 25 genotypes of a family, summing up to 75 genotypes tested per family. Two susceptible cultivars were included in the experiment as checks. The genotypes were evaluated for insect resistance, tuber yield traits, tuber appearance and tuber skin smoothness. The genetic value for the intensity of insect attack in the leaves and in the tubers was lower(higher resistance) in the 11 families than in the checks. For yield traits, there was no predominant effect among the families regarding the origin of the resistant donor species(Solanum berthaultii and/or S. chacoense). However, there was a large contribution of the C-1485-16-87 recurrent parent in crosses with resistant genotypes for tuber yield and tuber number. In relation to tuber appearance, only the family derived form the C-1485-16-87/ ND140(S. berthaultii) cross did not differ from the checks. had genetic value tuber skin smoothness, it was identified that families derived from S. berthaultii resistant parents showed higher genetic values than the check cultivars. For tuber skin smoothness, the genetic values of the families neither differed significantly from each other nor from the checks.

      • KCI등재후보

        Insect Resistance and Horticultural Trait Genetic Values of Potato Families

        Velci Queiróz de Souza,Arione da Silva Pereira,Giovani Olegário da Silva,Fernando Irajá Félix de Carvalho,Antônio Costa de Oliveira 한국작물학회 2008 Journal of crop science and biotechnology Vol.11 No.1

        This work aimed to evaluate 11 potato families for insect resistance horticultural traits. The families were derived from crosses between introduced insect resistant and adapted genotypes. A randomized complete block design, with three replications was used. The plot consisted of 25 genotypes of a family, summing up to 75 genotypes tested per family. Two susceptible cultivars were included in the experiment as controls. The genotypes were evaluated for insect resistance, tuber yield traits, tuber appearance, and tuber skin smoothness. The genetic value for the intensity of insect attack in the leaves and in the tubers was lower (higher resistance) in the 11 families than in the controls. For yield traits, there was no predominant effect among the families regarding the origin of the resistant donor species (Solanum berthaultii and/or S. chacoense). However, there was a large contribution of the C-1485-16- 87 recurrent parent in crosses with resistant genotypes for tuber yield and tuber number. In relation to tuber appearance, only the family derived form the C-1485-16-87/ND140 (S. berthaultii) cross did not differ from the controls. For tuber skin smoothness, the genetic values of the families did not significantly from each other or from the controls. This work aimed to evaluate 11 potato families for insect resistance horticultural traits. The families were derived from crosses between introduced insect resistant and adapted genotypes. A randomized complete block design, with three replications was used. The plot consisted of 25 genotypes of a family, summing up to 75 genotypes tested per family. Two susceptible cultivars were included in the experiment as controls. The genotypes were evaluated for insect resistance, tuber yield traits, tuber appearance, and tuber skin smoothness. The genetic value for the intensity of insect attack in the leaves and in the tubers was lower (higher resistance) in the 11 families than in the controls. For yield traits, there was no predominant effect among the families regarding the origin of the resistant donor species (Solanum berthaultii and/or S. chacoense). However, there was a large contribution of the C-1485-16- 87 recurrent parent in crosses with resistant genotypes for tuber yield and tuber number. In relation to tuber appearance, only the family derived form the C-1485-16-87/ND140 (S. berthaultii) cross did not differ from the controls. For tuber skin smoothness, the genetic values of the families did not significantly from each other or from the controls.

      • KCI등재

        Impact of Genetically Modified Enterobacter cloacae on Indigenous Endophytic Community of Citrus sinensis Seedlings

        Fernando Dini Andreote,Marcelo Jose Mortatti Gullo,Andre Oliveira de Souza Lima,Walter Maccheroni Junior,Joao Lucio Azevedo,Welington Luiz Araujo 한국미생물학회 2004 The journal of microbiology Vol.42 No.3

        Enterobacter cloacae (strain PR2/7), a genetically modified endophyte (GME) in citrus plants, carrying different plasmids (pEC3.0/18, pCelE, pEglA and pGFP), was inoculated into Citrus sinensis seedlings under greenhouse conditions. The impact of this on the indigenous bacterial endophytic community was studied by analyses of 2 different morphologic groups. The germination rates of inoculated seeds were evaluated in greenhouse, and plasmid stability under in vitro conditions. Results demonstrated a great and diverse endophytic community inside plants, and specialization in tissue colonization by some bacterial groups, in different treatments. Shifts in seed germination rate were observed among treatments: in general, the PR2/7 harboring pEglA bacterial clone significantly reduced seed germination, compared to the PR2/7 harboring pEC3.0/18 clone. This suggests that the presence of the pEglA plasmid changes bacteria-seed interactions. The endophytic community of citrus seedlings changed according to treatment. In seedlings treated with the PR2/7 with pEglA clone, the population of group II decreased significantly, within the context of the total endophytic community. These results indicate that the application of GMEs induces shifts in the endophytic bacterial community of citrus seedlings.

      • KCI등재

        Control and Reorganization of Heterogeneous Vehicle Platoons after Vehicle Exits and Entrances

        Daniel Almeida Godinho,Armando Alves Neto,Leonardo Amaral Mozelli,Fernando de Oliveira Souza 제어·로봇·시스템학회 2022 International Journal of Control, Automation, and Vol.20 No.8

        Platoons are efficient solutions to improve road transportation, traffic throughput, and other demands. But problems such as environmental disturbances, network imperfections, and unaware surrounding vehicles increase the necessity for resilient methods that ensure stability and connectivity among the platoon members. Hence, in this paper, we study the problem of decentralized control for platoons of heterogeneous vehicles under a limited communication range with a constant spacing policy. The goals are to guarantee inter-vehicle distances in steady-state, and resourcefulness to overcome entrances and exits of vehicles. Our main result is a resilient strategy, composed of a reconfiguration protocol and some simple rules for tuning the control law, based on local information about the relative connectivity to neighbors. It guarantees, in a decentralized manner, the automatic reorganization of the platoon under the entry and exit of agents. We conclude by illustrating our approach with simulated experiments on platoons with different numbers of vehicles, and by comparing it with the current state-of-the-art.

      • KCI등재

        Molecular diversity of the VP2 of Carnivore protoparvovirus 1 (CPV-2) of fecal samples from Bogotá

        Cristian Camilo Galvis,Tatiana Jimenez-Villegas,Diana Patricia Reyes Romero,Alejandro Velandia,Sueli Taniwaki,Sheila Oliveira de Souza Silva,Paulo Brandão,Nelson Fernando Santana-Clavijo 대한수의학회 2022 Journal of Veterinary Science Vol.23 No.1

        Background: Carnivore protoparvovirus 1, also known as canine parvovirus type 2 (CPV-2), is the main pathogen in hemorrhagic gastroenteritis in dogs, with a high mortality rate. Three subtypes (a, b, c) have been described based on VP2 residue 426, where 2a, 2b, and 2c have asparagine, aspartic acid, and glutamic acid, respectively. Objectives: This study examined the presence of CPV-2 variants in the fecal samples of dogs diagnosed with canine parvovirus in Bogotá. Methods: Fecal samples were collected from 54 puppies and young dogs (< 1 year) that tested positive for the CPV through rapid antigen test detection between 2014–2018. Molecular screening was developed for VP1 because primers 555 for VP2 do not amplify, it was necessary to design a primer set for VP2 amplification of 982 nt. All samples that were amplified were sequenced by Sanger. Phylogenetics and structural analysis was carried out, focusing on residue 426. Results: As a result 47 out of 54 samples tested positive for VP1 screening, and 34/47 samples tested positive for VP2 980 primers as subtype 2a (n = 30) or 2b (n = 4); subtype 2c was not detected. All VP2 sequences had the amino acid, T, at 440, and most Colombian sequences showed an S514A substitution, which in the structural modeling is located in an antigenic region, together with the 426 residue. Conclusions: The 2c variant was not detected, and these findings suggest that Colombian strains of CPV-2 might be under an antigenic drift.

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