Prevalence and Risk Factors of Cerebral Small Vessel Disease in a Chinese Population-Based Sample

Fei Han,Fei-Fei Zhai,Quan Wang,Li-Xin Zhou,Jun Ni,Ming Yao,Ming-Li Li,Shu-Yang Zhang,Li-Ying Cui,Zheng-Yu Jin,Yi-Cheng Zhu 대한뇌졸중학회 2018 Journal of stroke Vol.20 No.2

Background and Purpose Epidemiological data of cerebral small vessel disease (CSVD) in the general population of China are lacking. We report on the prevalence of lacunes, white matter hyperintensity (WMH), and cerebral microbleeds (CMBs) in a community-based sample in China and compare the results with those of other studies. Methods This was a cross-sectional analysis of the population-based Shunyi Study in China. A total of 1,211 stroke-free participants (mean age, 55.6±9.3 years; 37.4% men) with available 3 Tesla (3T) magnetic resonance images were included in this analysis. Demographic information and risk factor data were assessed. The overall and age-specific prevalence of lacunes, WMH, and CMBs was evaluated. Associations between cardiovascular risk factors and the presence of these lesions were analyzed by multiple logistic regression. Results Our study showed a prevalence of 14.5% for lacunes, 72.1% for periventricular hyperintensity (PVH), 65.4% for deep white matter hyperintensity (DWMH), and 10.6% for CMBs. When compared with other community-based samples, individuals in the same age group showed a higher burden of lacunes and a relatively lower prevalence of CMBs. Advanced age was independently associated with the prevalence of these CSVD markers, while the presence of hypertension increased the risk of lacunes, PVH/DWMH, and CMBs in deep or infratentorial locations. Conclusions A higher burden of lacunes but a relatively lower prevalence of CMBs was observed in this Chinese population. This notable result highlights the challenge of CSVD prevention in China. Chinese have a risk factor profile for CSVD similar to those in other populations.

Effects of the NQO1 609C>T Polymorphism on Leukemia Susceptibility: Evidence from a Meta-analysis

Han, Fei-Fei,Guo, Chang-Long,Gong, Li-Li,Jin, Zhu,Liu, Li-Hong Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.9

A functional polymorphism in the NQO1 gene, featuring a 609C>T substitution,leading to proline to serine amino-acid and enzyme activity changes, has been implicated in cancer risk. However, individually published investigations showed inconclusive results, especially for leukemia. In this study, we therefore performed a meta-analysis of 21 publications with a total of 3,634 cases and 4,827controls, mainly for leukemia. We summarized the data on the association between the NQO1 609C>T polymorphism and risk of leukemia and performed subgroup analyses by ethnicity and leukemia type. We found that the variant TT homozygous genotype o was associated with a modestly increased risk of leukemia (TT versus CT/CC: OR=1.23, 95%CI=1.00-1.51, heterogeneity=0.76; $I^2$=0%). Following further stratified analyses, increased risk was only observed in subgroups of Caucasians. This meta-analysis suggests that the NQO1 609T allele is a high-penetrance risk factor for leukemia in Caucasians. The effect on leukemia may be modified by ethnicity and leukemia type, and the small sample sizes of the subgroup analyses suggest that further larger studies are needed.

Hsp90 Inhibitor Geldanamycin Enhances the Antitumor Efficacy of Enediyne Lidamycin in Association with Reduced DNA Damage Repair

Han, Fei-Fei,Li, Liang,Shang, Bo-Yang,Shao, Rong-Guang,Zhen, Yong-Su Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.17

Inhibition of heat shock protein 90 (Hsp90) leads to inappropriate processing of proteins involved in DNA damage repair pathways after DNA damage and may enhance tumor cell radio- and chemotherapy sensitivity. To investigate the potentiation of antitumor efficacy of lidamycin (LDM), an enediyne agent by the Hsp90 inhibitorgeldanamycin (GDM), and possible mechanisms, we have determined effects on ovarian cancer SKOV-3, hepatoma Bel-7402 and HepG2 cells by MTT assay, apoptosis assay, and cell cycle analysis. DNA damage was investigated with H2AX C-terminal phosphorylation (${\gamma}H2AX$) assays. We found that GDM synergistically sensitized SKOV-3 and Bel-7402 cells to the enediyne LDM, and this was accompanied by increased apoptosis. GDM pretreatment resulted in a greater LDM-induced DNA damage and reduced DNA repair as compared with LDM alone. However, in HepG2 cells GDM did not show significant sensitizing effects both in MTT assay and in DNA damage repair. Abrogation of LDM-induced $G_2/M$ arrest by GDM was found in SKOV-3 but not in HepG2 cells. Furthermore, the expression of ATM, related to DNA damage repair responses, was also decreased by GDM in SKOV-3 and Bel-7402 cells but not in HepG2 cells. These results demonstrate that Hsp90 inhibitors may potentiate the antitumor efficacy of LDM, possibly by reducing the repair of LDM-induced DNA damage.

An Epigenetic Mechanism Underlying Doxorubicin Induced EMT in the Human BGC-823 Gastric Cancer Cell

Han, Rong-Fei,Ji, Xiang,Dong, Xing-Gao,Xiao, Rui-Jing,Liu, Yan-Ping,Xiong, Jie,Zhang, Qiu-Ping Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.10

The epithelial to mesenchymal transition (EMT) is a key step during embryonic morphogenesis and plays an important role in drug resistance and metastasis in diverse solid tumors. We previously reported that 48 h treatment of anti-cancer drug doxorubicin could induce EMT in human gastric cancer BGC-823 cells. However, the long term effects of this transient drug treatment were unknown. In this study we found that after 48 h treatment with $0.1{\mu}g/ml$ doxorubicin, most cells died during next week, while a minor population of cells survived and formed colonies. We propagated the surviving cells in drug free medium and found that these long term cultured drug survival cells (abbreviated as ltDSCs) retained a mesenchymal-like cell morphology, and expressed high levels of EMT-related molecules such as vimentin, twist and ${\beta}$-catenin. The expression of chromatin reprogramming factors, Oct4 and c-myc, were also higher in ltDSCs than parental cells. We further demonstrated that the protein level of p300 was upregulated in ltDSCs, and inhibition of p300 by siRNA suppressed the expression of vimentin. Moreover, the ltDSCs had higher colony forming ability and were more drug resistant when compared to parental cells. Our results suggested that an epigenetic mechanism is involved in the EMT of ltDSCs.

An Ultra-narrow Bandwidth Filter for Daytime Wind Measurement of Direct Detection Rayleigh Lidar

Fei Han,Hengjia Liu,Dongsong Sun,Yuli Han,Anran Zhou,Nannan Zhang,Jiaqi Chu,Jun Zheng,Shan Jiang,Yuanzu Wang 한국광학회 2020 Current Optics and Photonics Vol.4 No.1

A Rayleigh Lidar used for wind detection works by transmitting laser pulses to the atmosphere and receiving backscattering signals from molecules. Because of the weak backscattering signals, a lidar usually uses a high sensitivity photomultiplier as detector and photon counting technology for signal collection. The capturing of returned extremely weak backscattering signals requires the lidar to work on dark background with a long time accumulation to get high signal-to-noise ratio (SNR). Because of the strong solar background during the day, the SNR of lidar during daytime is much lower than that during nighttime, the altitude and accuracy of detection are also restricted greatly. Therefore this article describes an ultra-narrow bandwidth filter (UNBF) that has been developed on 354.7 nm wavelength of laser. The UNBF is used for suppressing the strong solar background that degrades the performance of Rayleigh wind lidar during daytime. The optical structure of UNBF consists of an interference filter (IF), a low resolution Fabry-Perot interferometer (FPI) and a high resolution FPI. The parameters of each optical component of the UNBF are presented in this article. The transmission curve of the aligned UNBF is measured with a tunable laser. Contrasting the result of with-UNBF and with-IF shows that the solar background received by a Licel transient recorder decreases by 50~100 times and that the SNR with-UNBF was improved by 3 times in the altitude range (35 km to 40 km) compared to with-IF at 10:26 to 10:38 on August 29, 2018. By the SNR comparison at four different times of one day, the ratio-values are larger than 1 over the altitude range (25~50 km) in general, the results illustrate that the SNR with-UNBF is better than that with-IF for Rayleigh Lidar during daytime and they demonstrate the effective improvements of solar background restriction of UNBF.

An Ultra-narrow Bandwidth Filter for Daytime Wind Measurement of Direct Detection Rayleigh Lidar

Han, Fei,Liu, Hengjia,Sun, Dongsong,Han, Yuli,Zhou, Anran,Zhang, Nannan,Chu, Jiaqi,Zheng, Jun,Jiang, Shan,Wang, Yuanzu Optical Society of Korea 2020 Current Optics and Photonics Vol.4 No.1

A Rayleigh Lidar used for wind detection works by transmitting laser pulses to the atmosphere and receiving backscattering signals from molecules. Because of the weak backscattering signals, a lidar usually uses a high sensitivity photomultiplier as detector and photon counting technology for signal collection. The capturing of returned extremely weak backscattering signals requires the lidar to work on dark background with a long time accumulation to get high signal-to-noise ratio (SNR). Because of the strong solar background during the day, the SNR of lidar during daytime is much lower than that during nighttime, the altitude and accuracy of detection are also restricted greatly. Therefore this article describes an ultra-narrow bandwidth filter (UNBF) that has been developed on 354.7 nm wavelength of laser. The UNBF is used for suppressing the strong solar background that degrades the performance of Rayleigh wind lidar during daytime. The optical structure of UNBF consists of an interference filter (IF), a low resolution Fabry-Perot interferometer (FPI) and a high resolution FPI. The parameters of each optical component of the UNBF are presented in this article. The transmission curve of the aligned UNBF is measured with a tunable laser. Contrasting the result of with-UNBF and with-IF shows that the solar background received by a Licel transient recorder decreases by 50~100 times and that the SNR with-UNBF was improved by 3 times in the altitude range (35 km to 40 km) compared to with-IF at 10:26 to 10:38 on August 29, 2018. By the SNR comparison at four different times of one day, the ratio-values are larger than 1 over the altitude range (25~50 km) in general, the results illustrate that the SNR with-UNBF is better than that with-IF for Rayleigh Lidar during daytime and they demonstrate the effective improvements of solar background restriction of UNBF.

The Improvement and Perfection of Civil Judicial Application System

Fei Han 한국유통과학회 2017 한국유통과학회 학술대회 논문집 Vol.2017 No.-

In the application of civil justice, the opinions of accounting should establish the basic principles of fairness and neutrality, and ensure the correct application in civil cases. In addition to sticking to the basic principles, it should also be used for accounting qualification, starting mode, Opinions on the application of civil justice system to improve and perfect. The principle of accounting appraisal in civil litigation mainly refers to the accounting appraisal opinion in the application process, combined with its own characteristics should comply with the guidelines, so as to protect the role of its evidence to play. In addition to some general principles, the author believes that the accounting of the views of the application of civil justice should follow the unique principles:

Miraculin gene transformation in embryogenic Citrus callus and regeneration

Fei Li,Seong Beom Jin,Myeung-Seung Kim,Song-I Han,Hyeon-Jin Sun,Jae Hoon Kim 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07

Miraculin can modify a sour taste into a sweet taste and is an alternative to traditional sweeteners. Mirculin gene was introduced into the Miyagawa Wase Satsuma mandarin (Citrus unshiu Marc.) callus by Agrobacterum-mediated transformation to produce citrus transgenic plant. Transgenic plant candidates were selected via hygromycin resistance. Transformation was confirmed by Polymerase Chain Reaction, Southern blotting, and Western blot analysis. Expression of this gene in transgenic citrus resulted in the accumulation of miraculin protein in the leaves. Multiple Shoots of transgenic citrus planets were micrografted onto trifoliate rootstocks in the sterile soil. Plants were established in the greenhouse 2 years after planting.

Numerical and Experimental Parametric Study on the Mechanism of Web-Warping in Chain-Die Forming with Variable Cross-Section

Fei Han,Zhen Qian,Yun Wang,Xiu-Kun Wang,Si-chao Mu,Shi-Chao Ding 한국정밀공학회 2023 International Journal of Precision Engineering and Vol.24 No.2

Chain-die forming is a new sheet metal forming process, which can be used to produce complex and variable cross-section UHSS automotive structural parts. In this paper, the web-warping and the longitudinal strain developed in the sheet metal during the chain-die forming process are investigated by the finite element analysis, and the accuracy of the model is verified by the experimental work by comparing the longitudinal strain of the product with a forming angle of 60°. The reasons for web-warping in chain-die forming and the effects of different process parameters on web-warping are analyzed. The results show that the side leg height has the most significant effect on the longitudinal strain development, followed by material strength, sheet metal thickness and turning radius. Likewise, the linear regression model confirms that the material strength has the most significant effect on the web-warping height while the forming angle and side leg height have less influence during chain-die forming variable cross-section profile.

Mechanical Bending Property of Ultra-High Strength Steel Sheets IN Roll Forming Process

Fei Han,Yun Wang,Zai-Lin Wang 한국정밀공학회 2018 International Journal of Precision Engineering and Vol.19 No.12

Ultra-high strength steel (UHSS) plays an important role in the automotive lightweight industry owing to its insubstantial weight and high specific strength. Roll forming is one of the effective forming processes for UHSS in the steel industry. In this study, the minimum bending radius is determined by roll forming experiments, taking into account the 1200MPa level of the V-profiled parts of UHSS. The ductile fracture criterion (DFC), which considers both the “tensile ductile fracture” and “shear ductile fracture,” is applied to predict the fracture of sheets in the roll forming process, where bending is the main deformation mode. A set of rolls is designed to investigate the fracture behavior in the roll forming process. In addition, finite element models (FEMs) are built and four sets of experiments are conducted to calibrate the fracture of the V-profiled parts for R/T = 1, 2, 3, and 4. Furthermore, the fracture time and fracture location of the V-profiled parts for R/T = 1 and 2 are predicted and the prediction results agree with the experimental results.