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RISS 인기검색어
- 검색키워드
- 저자 : Erbersdobler, Andreas (검색결과 3 건)
- 무료
- 기관 내 무료
- 유료
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Studies on Heated Protein Quality Using Homoarginine Method
Kyung-Hea Lee,Helmut Erbersdobler 한국식품영양과학회 1996 Preventive Nutrition and Food Science Vol.1 No.1
To determine the quality of heated protein, in vitro method, including lysine, lysinoalanine, and fructoselysine as well as homoarginine by guanidination of lysine, was assessed using heated casein with or without glucose. In vivo methods such as PER, digestibility and BV were also tried on homoarginine, lysinoalanine, fructoselysine, and lysine. The nonreactive lysine for guanidination was hardly digestive, while the non heat damaged lysine side chains in the protein were accessible for guanidination as well as for the digestion. A linear correlation(r=0.80) was obtained between PER and digestibility of the analysed lysine. Digestibility of homoarginine was higher than that of true protein. However, in the guanidinated heated casein with glucose, digestibility of homoarginine was significantly reduced. It is suggested that the homoarginine method may mislead to over- or underestimation of the damaged protein quality.
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Suitable reference genes for relative quantification of miRNA expression in prostate cancer
Annika Schaefer,Monika Jung,Kurt Miller,Michael Lein,Glen Kristiansen,Andreas Erbersdobler,Klaus Jung 생화학분자생물학회 2010 Experimental and molecular medicine Vol.42 No.11
Real time quantitative PCR (qPCR) is the method of choice for miRNA expression studies. For relative quantification of miRNAs, normalization to proper reference genes is mandatory. Currently, no validated reference genes for miRNA qPCR in prostate cancer are available. In this study, the expression of four putative reference genes (hsa-miR-16, hsa-miR-130b, RNU6-2,SNORD7) was examined with regard to their use as normalizer. After SNORD7 was already shown an inappropriate reference gene in preliminary experiments using total RNA pools, we studied the expression of the putative reference genes in tissue and normal adjacent tissue sample pairs from 76 men with untreated prostate carcinoma collected after radical prostatectomy. hsa-miR-130b and RNU6-2 showed no significantly different expression between the matched malignant and non-malignant tissue samples, whereas hsa-miR-16was significantly underexpressed in malignant tissue. Softwares geNorm and Normfinder predicted hsamiR-130b and the geometric mean of hsa-miR-130b and RNU6-2 as the most stable reference genes. Normalization of the four miRNAs hsa-miR-96, hsamiR-125b, hsa-miR-205, and hsa-miR-375, which were previously shown to be regulated, shows that normalization to hsa-mir-16 can lead to biased results. We recommend using hsa-miR-130b or the geometric mean of hsa-miR-130b and small RNA RNU6-2 for normalization in miRNA expression studies of prostate cancer.
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Suitable reference genes for relative quantification of miRNA expression in prostate cancer
Schaefer, Annika,Jung, Monika,Miller, Kurt,Lein, Michael,Kristiansen, Glen,Erbersdobler, Andreas,Jung, Klaus Korean Society for Biochemistry and Molecular Bion 2010 Experimental and molecular medicine Vol.42 No.11
Real time quantitative PCR (qPCR) is the method of choice for miRNA expression studies. For relative quantification of miRNAs, normalization to proper reference genes is mandatory. Currently, no validated reference genes for miRNA qPCR in prostate cancer are available. In this study, the expression of four putative reference genes (hsa-miR-16, hsa-miR-130b, RNU6-2, SNORD7) was examined with regard to their use as normalizer. After SNORD7 was already shown an inappropriate reference gene in preliminary experiments using total RNA pools, we studied the expression of the putative reference genes in tissue and normal adjacent tissue sample pairs from 76 men with untreated prostate carcinoma collected after radical prostatectomy. hsa-miR-130b and RNU6-2 showed no significantly different expression between the matched malignant and non-malignant tissue samples, whereas hsa-miR-16 was significantly underexpressed in malignant tissue. Softwares geNorm and Normfinder predicted hsamiR-130b and the geometric mean of hsa-miR-130b and RNU6-2 as the most stable reference genes. Normalization of the four miRNAs hsa-miR-96, hsamiR-125b, hsa-miR-205, and hsa-miR-375, which were previously shown to be regulated, shows that normalization to hsa-mir-16 can lead to biased results. We recommend using hsa-miR-130b or the geometric mean of hsa-miR-130b and small RNA RNU6-2 for normalization in miRNA expression studies of prostate cancer.
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