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Preparation of GF37 for the improvement of respiratory inflammation
Ju Yeong So,Min Jung Kim,Go Woo Choi,You Jin Lee,Yun Ho Jo,Sejeong Kim,Danhui Kim,Sang Hun Lee,Een Yeong Park,Dae Seok Yoo,Jin Seong Kim,Sang Won Cho,Young Soo Kim,Hak Joo Choi,Mi Kyung Pyo 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10
Panax ginseng (PG) and Perilla frutesens (PF) leaves have antioxidant and anti-inflammatory action, which can alleviate various diseases caused by fine dust. Therefore, the purpose of this study is to find the optimal ratio of ginseng and perilla leaves to maximize the anti-inflammatory action. PG and PF were extracted three times for 6 h at 70℃ with 70% ethanol and concentrated in vacuum, respectively, and then powdered by spray dryer. PG:PF were each made at different ratios to make 9 samples; CHJ1 (1:9), CHJ2 (2:8), CHJ3 (3:7), CHJ4 (4:6), CHJ5 (5:5), CHJ6 (6:4), CHJ7 (7:3), CHJ8 (8:2), CHJ9 (9:1). Each sample was measured TNF-a, IL-6, and ROS against fine dust (PM10) using MH-S cells. Among the 9 mixed samples, CHJ2 and CHJ3 inhibited the inflammation and oxidative stress induced by fine dust the best. In a confirmation experiment comparing these samples with PG and PF samples, the CHJ2 sample was finally selected because it had the best anti-inflammatory and antioxidant effects at a low concentration. Finally, a CHJ2 sample in which PG and PF were mixed in a 3:7 ratio was selected as a material for improving respiratory inflammation, and was named GF37.