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      • 漢江流域 豪雨의 空間特性

        鄭斗永,李在炯 全北大學校 1987 論文集 Vol.29 No.-

        By the spectral analysis of many heavy rains' recorded data, at Han river basin, an emprical spectral density function model is presented for the spatial distribution of storm. Using the storm data which was augmented by the Stochastic correlation with it's neighbors, the multiquadric random ndom surface of total storm depth is constructed. And to separate the local components from it's regionals and find the regional charcteristics, a double Fourier analysis was applied to the total depths. The local components, storm residuals of each storm was assumed to be an homogeneous random field and investigated with it's autocorrelation function. For the practical application, isotropic was assumed which was identifyed with emprical data. Coefficients of normalized autocorrelation for all storms were shown similar appearance. Using this emprical result, an example of the radial spectral density function is presented which represents the spatial characteristics of heavyrains over Han-river basin during 1975~1983.

      • 高等學校 生物 實驗實習敎材의 開發硏究 Ⅰ

        崔斗文,李元求,文炯泰 공주대학교 사범대학 과학교육연구소 1984 과학교육연구 Vol.16 No.1

        Teaching materials available for the effective performance of laboratory experimental work in high school biology were developed, laying emphasis on the following items : environmental pollution and natural conservations, biological concepts that the degree of difficulty is high, applications of teaching materials which are developed in foreign conturies, productions of teaching materials and models. The measuring methods of size of micrography without micrometer or with a hand-made glass micrometer were developed. By observing the morphological characters of liverwort, the methods of telling its sex, distingshing its life cycle into sexual reproduction and asexual reproduction, and comparing its life cycle with those of other plants were presented. By observing the morphological characters of moss, the methods of telling its sex and comparing its life cycle with those of liverwork, ferns and flowering plants were presented. A convenient observing method of cartilaginous and bony tissues in animals was developed using the easily available material in our surrounding The observing method of extension and contraction of chromatophores induced by environmental changes on freshwater fishes was developed. The methods of sorting and identifying the higher plants by observing their leaves and flowers were presented. The improved analytical method of plant community structure was presented by using the artificial plant community within laboratory. The assesing method of harmful effects on plant tissues by sulfur dioxide was improved.

      • KCI등재

        Rapid Identification and Validation of Xanthomonas oryzae pv. oryzae (Xoo) by using PCR-amplified Phage Integrase and Transposase A Gene

        Hyeong-Kwon Shim,Tae-Hwan Noh,Mi-Hyung Kang,Young-Jin Park,Du-Ku Lee,Byoung Moo Lee,Kuldeep Tyagi,Chae-Hoon Paik,Geon-Hwi Lee 한국국제농업개발학회 2012 韓國國際農業開發學會誌 Vol.24 No.2

        벼 흰잎마름병균의 정확한 진단을 위하여 PCR용 진단 kit를 개발하였다. 본 PCR kit를 개발하기 위하여 벼 흰잎마름 병균 유전체 정보 중 phage-related integrase and transposase gene의 염기서열을 이용하여 프라이머를 각각 제작하였다. 프라이머 염기서열은 XOP-F (5-CGG TCT GCT CAA TGA GGA AGA-3)와 XOP-R2 (5-TGC AAT TGG TGT TCTCCA GG-3), XOT-F (5-GTC ATA GGT GAG GCT TCCC-3)와 XOT-R2 (5-AGT GCG ATC TTT CAG CAG G-3)로 벼 흰잎마름병균의 DNA를 401bp와 492bp를 증폭하게 제작하였다. PCR 증폭은 벼 흰잎마름병균만 증폭하였으며 다른 세균인 Escherichia coli, Agrobacterim, Pectobacterium caratovora subsp. cartovorum, P. atrosepticum, Pseudomonasputida, P. syringae, P. savastanoi pv. phaeolicola, P. savastanoipv. savastanoi and P. marginalis pv. Marginalis 등은증폭되지 않아 특이성이 인정 되었다. 본 프라이머로 병이 의심되는 벼잎과 논물에서 병원균을 3시간 이내에 검출할 수 있었다. PCR-based specific identification of Xanthomonas oryzae pv. oryzae (Xoo), the pathogen responsible for bacterial blight (BB) of rice, was developed by amplifying the Xoo-specific phage-related integrase and transposase gene. Therefore, dual primers, XOP-F (5-CGGTCTGCTCAATGAGGAAGA-3) and XOP-R2 (5-TGCAATTGGTGTTCTCCAGG-3) and XOT-F (5-GTCATAGGTGAGGCTTCCC-3) and XOT-R2 (5-AGTGCGATCTTTCAGCAGG-3) were designed and found to specifically amplify 401bp and 492bp fragments from all strains of Xoo isolates from diverse regions in Korea. The PCR products were only amplified from Xoo among other bacterial strains including Escherichia coli, Agrobacterim, Pectobacterium caratovora subsp. cartovorum, P. atrosepticum, Pseudomonas putida, P. syringae, P. savastanoi pv. phaeolicola, P. savastanoi pv. savastanoi and P. marginalis pv. marginalis. This method could also be applied to detect the pathogen in infected rice leaves and paddy field water within 3 hr.

      • SCIESCOPUSKCI등재

        Accuracy of a direct drill-guiding system with minimal tolerance of surgical instruments used for implant surgery: a prospective clinical study

        Du-Hyeong Lee,Seo-Young An,Min-Ho Hong,Kyoung-Bae Jeon,Kyu-Bok Lee 대한치과보철학회 2016 The Journal of Advanced Prosthodontics Vol.8 No.3

        PURPOSE A recently introduced direct drill-guiding implant surgery system features minimal tolerance of surgical instruments in the metal sleeve by using shank-modified drills and a sleeve-incorporated stereolithographic guide template. The purpose of this study was to evaluate the accuracy of this new guided surgery system in partially edentulous patients using geometric analyses. MATERIALS AND METHODS For the study, 21 implants were placed in 11 consecutive patients using the direct drill-guiding implant surgery system. The stereolithographic surgical guide was fabricated using cone-beam computed tomography, digital scanning, computer-aided design and computer-assisted manufacturing, and additive manufacturing processes. After surgery, the positional and angular deviations between planned and placed implants were measured at the abutment level using implant-planning software. The Kruskal-Wallis test and Mann-Whitney U test were used to compare the deviations (α=.05). RESULTS The mean horizontal deviations were 0.593 mm (SD 0.238) mesiodistally and 0.691 mm (SD 0.344) buccolingually. The mean vertical deviation was 0.925 mm (SD 0.376) occlusogingivally. The vertical deviation was significantly larger than the horizontal deviation (P=.018). The mean angular deviation was 2.024 degrees (SD 0.942) mesiodistally and 2.390 degrees (SD 1.142) buccolingually. CONCLUSION The direct drill-guiding implant surgery system demonstrates high accuracy in placing implants. Use of the drill shank as the guiding component is an effective way for reducing tolerance

      • Acid-Promoted Crystallization of Amorphous Solid Water

        Lee, Du Hyeong,Kang, Heon American Chemical Society 2018 JOURNAL OF PHYSICAL CHEMISTRY C - Vol.122 No.42

        <P>In this study, the effect of externally added hydrogen chloride (HCl) on the crystallization of amorphous solid water (ASW) has been examined. ASW films containing small amounts of HCl, which dissociated into H<SUP>+</SUP> (excess protons) and Cl<SUP>-</SUP> ions, and having a thickness of 90-360 monolayers (ML) were prepared on a Pt(111) substrate under an ultrahigh vacuum environment. The location of HCl in the samples was varied by controlling the stacking sequence of H<SUB>2</SUB>O and HCl during the film growth. Crystallization kinetics of these samples were examined by conducting temperature-programmed desorption experiments and isothermal kinetic measurements with reflection-absorption infrared spectroscopy for the temperature range of 137-145 K. Crystallization behaviors of pure and NaCl-doped ASW films were also examined for comparison. The results indicated that the excess protons accelerated the crystallization of the ASW films, in contrast to the retardation effect of Na<SUP>+</SUP>. In the presence of 0.1 ML HCl, the overall activation energy of ASW crystallization was reduced from 63.4 kJ·mol<SUP>-1</SUP> in the absence of HCl to 48.5 kJ·mol<SUP>-1</SUP>, which is close to the activation energy of crystal growth. The crystallization started near the location of HCl injection in the samples, regardless of whether it was the vacuum/ASW interface or the film interior. These observations indicated that the excess protons facilitated the nucleation process and changed the rate-limiting step of ASW crystallization from nucleation to growth. An explanation based on thermodynamics has been proposed, that is, the configurational entropy of the excess protons in ice likely reduces the free-energy barrier of nucleation for the acid-doped ASW samples.</P> [FIG OMISSION]</BR>

      • SCIESCOPUSKCI등재

        The effect of 4,4'-bis(N,Ndiethylamino) benzophenone on the degree of conversion in liquid photopolymer for dental 3D printing

        Du-Hyeong Lee,Hang Nga Mai,Jin-Chul Yang,Tae-Yub Kwon 대한치과보철학회 2015 The Journal of Advanced Prosthodontics Vol.7 No.5

        PURPOSE. The purpose of this preliminary study was to investigate the effects of adding 4,4'-bis(N,Ndiethylamino) benzophenone (DEABP) as a co-initiator to a binary photoinitiating system (camphorquinoneamine) to analyze on the degree of conversion (DC) of a light-cured resin for dental 3D printing. MATERIALS AND METHODS. Cylindrical specimens (N=60, n=30 per group, ø5 mm × 1 mm) were fabricated using bisphenol A glycerolate dimethacrylate (BisGMA) both with and without DEABP. The freshly mixed resins were exposed to light in a custom-made closed chamber with nine light-emitting diode lamps (wavelength: 405 nm; power: 840 mW/cm2) for polymerization at each incidence of light-irradiation at 10, 30, 60, 180, and 300 seconds, while five specimens at a time were evaluated at each given irradiation point. Fourier-transform infrared (FTIR) spectroscopy was used to measure the DC values of the resins. Two-way analysis of variance and the Duncan post hoc test were used to analyze statistically significant differences between the groups and given times (α=.05). RESULTS. In the DEABP-containing resin, the DC values were significantly higher at all points in time (P<.001), and also the initial polymerization velocity was faster than in the DEABP-free resin. CONCLUSION. The addition of DEABP significantly enhanced the DC values and, thus, could potentially become an efficient photoinitiator when combined with a camphorquinone?amine system and may be utilized as a more advanced photopolymerization system for dental 3D printing.

      • SCIESCOPUSKCI등재

        The effect of 4,4'-bis(N,N-diethylamino)benzophenone on the degree of conversion in liquid photopolymer for dental 3D printing

        Lee, Du-Hyeong,Mai, Hang Nga,Yang, Jin-Chul,Kwon, Tae-Yub The Korean Academy of Prosthodonitics 2015 The Journal of Advanced Prosthodontics Vol.7 No.5

        PURPOSE. The purpose of this preliminary study was to investigate the effects of adding 4,4'-bis(N,N-diethylamino) benzophenone (DEABP) as a co-initiator to a binary photoinitiating system (camphorquinone-amine) to analyze on the degree of conversion (DC) of a light-cured resin for dental 3D printing. MATERIALS AND METHODS. Cylindrical specimens (N=60, n=30 per group, ${\phi}5mm{\times}1mm$) were fabricated using bisphenol A glycerolate dimethacrylate (BisGMA) both with and without DEABP. The freshly mixed resins were exposed to light in a custom-made closed chamber with nine light-emitting diode lamps (wavelength: 405 nm; power: $840mW/cm^2$) for polymerization at each incidence of light-irradiation at 10, 30, 60, 180, and 300 seconds, while five specimens at a time were evaluated at each given irradiation point. Fourier-transform infrared (FTIR) spectroscopy was used to measure the DC values of the resins. Two-way analysis of variance and the Duncan post hoc test were used to analyze statistically significant differences between the groups and given times (${\alpha}$=.05). RESULTS. In the DEABP-containing resin, the DC values were significantly higher at all points in time (P<.001), and also the initial polymerization velocity was faster than in the DEABP-free resin. CONCLUSION. The addition of DEABP significantly enhanced the DC values and, thus, could potentially become an efficient photoinitiator when combined with a camphorquinone-amine system and may be utilized as a more advanced photopolymerization system for dental 3D printing.

      • SCIESCOPUSKCI등재

        Evaluation of internal fit of interim crown fabricated with CAD/CAM milling and 3D printing system

        Lee, Wan-Sun,Lee, Du-Hyeong,Lee, Kyu-Bok The Korean Academy of Prosthodonitics 2017 The Journal of Advanced Prosthodontics Vol.9 No.4

        PURPOSE. This study is to evaluate the internal fit of the crown manufactured by CAD/CAM milling method and 3D printing method. MATERIALS AND METHODS. The master model was fabricated with stainless steel by using CNC machine and the work model was created from the vinyl-polysiloxane impression. After scanning the working model, the design software is used to design the crown. The saved STL file is used on the CAD/CAM milling method and two types of 3D printing method to produce 10 interim crowns per group. Internal discrepancy measurement uses the silicon replica method and the measured data are analyzed with One-way ANOVA to verify the statistic significance. RESULTS. The discrepancy means (standard deviation) of the 3 groups are $171.6\;(97.4){\mu}m$ for the crown manufactured by the milling system and 149.1 (65.9) and $91.1\;(36.4){\mu}m$, respectively, for the crowns manufactured with the two types of 3D printing system. There was a statistically significant difference and the 3D printing system group showed more outstanding value than the milling system group. CONCLUSION. The marginal and internal fit of the interim restoration has more outstanding 3D printing method than the CAD/CAM milling method. Therefore, the 3D printing method is considered as applicable for not only the interim restoration production, but also in the dental prosthesis production with a higher level of completion.

      • SCIEKCI등재

        Induction of Systemic Resistance in Watermelon to Gummy Stem Rot by Plant Growth-Promoting Rhizobacteria

        Lee, Yong-Hoon,Lee, Wang-Hyu,Shim, Hyeong-Kwon,Lee, Du-Ku The Korean Society of Plant Pathology 2000 Plant Pathology Journal Vol.16 No.6

        The selected five plant growth-promoting rhizobacteria (PGPR) strains, WR8-3 (Pseudomonas fluorescens), WR8-6 (P. putida), WR9-9 (P. fluorescens), WR9-11 (Pseudomonas sp.), and WR9-16 (P. putida) isolated in the rhizosphere of watermelon plants were tested on their growth promotion and control effect against gummy stem rot of watermelon. Strains, WR8-3 and WR9-16 significantly increased stem length of watermelon, and there was a little increase in leaf area, fresh weight and root length when strains, WR8-3, WR9-9 and WR9-16 were treated. Generally, seed treatment was better for plant growth promotion than the soil drench, but there was no significant difference. Seed treatment and soil drench of each bacterial strain also significantly reduced the mean lesion area (MLA) by gummy stem rot, but there was no significant difference between the two treatments. At initial inoculum densities of each strain ranging from 10$^6\;to\;10^{15}$ cfu/g seed, approximately the same level of disease resistance was induced. But resistance induction was not induced at the initial inoculum density of 10$^3$ cfu/g seed. Resistance was induced by treating the strains, WR9-9, WR9-11 and WR9-16, on all of four watermelon varieties tested, and there was no significant difference in the decrease of gummy stem rot among varieties. Populations of the strains treated initially at log 9-10 cfu/g seed, followed with a rapid decrease from planting day to 1 week after planting, but the population density was maintained above log 5.0 cfu/g soil until 4 weeks after planting. Generally no or very weak in vitro antagonism was observed at the strains treated excepting WR9-11. Rifampicin-resistant bacteria which had been inoculated were not detected in the stems or leaves, which suggesting that the bacterium and the pathogens remained spatially separated during the experiment. This is the first report of rsistance induction in watermelon to gummy stem rot by PGPR strains.

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