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A Novel Marine Bacterium Vibrio sp. Dhg as a Promising Host Strain for Brown Macroalgae Refinery
Dongyeop BAEK,Hyun Gyu LIM,Dong Hun KWAK,Sungwoo PARK,Sunghwa WOO,Jae-Seong YANG,Chae Won KANG,Beomhee KIM,Myung Hyun NOH,Sang Woo SEO,Gyoo Yeol JUNG 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
Although brown macroalgae holds potential as an alternative feedstock, its utilization by conventional microbial platforms has been limited due to the inability to metabolize one of the principal sugars, alginate. Here, we isolate Vibrio sp. dhg, a fast-growing bacterium that can efficiently assimilate alginate. Based on systematic characterization of the genomic information of Vibrio sp. dhg, we establish a genetic toolbox for its engineering. We also demonstrate its ability to rapidly produce ethanol, 2,3-butanediol, and lycopene from brown macroalgae sugar mixture with high productivities and yields. Collectively, Vibrio sp. dhg can be used as a platform for the efficient conversion of brown macroalgae sugars into diverse value-added biochemicals.
플라이애시 품질이 시멘트 페이스트 레올로지 특성에 미치는 영향
백병훈 ( Baek Byung-hoon ),한동엽 ( Han Dongyeop ) 한국건축시공학회 2017 한국건축시공학회지 Vol.17 No.2
플라이애시는 시멘트 계열 재료의 혼화재료로서 널리 사용되고 있는 재료이다. 다만, 플라이애시는 화력발전소의 석탄재를 포집하여 정제과정을 거친 재료로서 정제과정의 에너지 소모 및 유통과정에서의 품질 확보가 중요하다. 이에 본 연구에서는 플라이애시 생산과정 중 발생하는 세 가지 종류의 다른 품질의 석탄재를 대상으로 각각의 입자조건과 시멘트 페이스트 상태에서 레올로지 거동을 평가하였다. 실험결과 리젝트 애시는 작은 석탄재 입자가 응집되어 굳어진 다공질의 큰 입자로 구성되어 있었고, 그러한 이유로 시멘트페이스트에 포함될 경우 공기량이 높게 측정되는 경향이 나타났다. 유동성 측면에서는 석탄재가 치환되는 경우 유동성이 증진되는 양상을 보였으며 특히 점도 측면에서는 입자조건에 따라 점도를 조정할 수도 있었다. The aim of the research is to provide rheological properties of cement paste with various qualities of coal ash including fly ash, raw ash, and reject ash. Generally, fly ash is the well known supplementrary cementitious materials for concrete and is used to improve various properties. Although fly ash is obtained as a byproduct of fire powder plant, still reject ash is wasted from raw ash. In this research, thus, to provide a fundamental information on using not only fly ash but also raw ash or reject ash for cementitious materials, a rheological properties of cement paste was studied with three different coal ash. This research was conducted from particle conditions of three different coal ashes to rheological properties in cement paste phase. According to the expeirment, reject ash was consisted with large and coagulated particles although fly ash was consisted with a small and spherical shaped particles. based on the particle conditions of various coal ashes, rheological behaviors were tested, and it was shown as the coal ashes improved the fluidity of cement paste. Specifically, depending on the particle distributions of cement paste, it is considered that the viscosity of paste can be controlled.
Wookyong Kwon,Jaemin Baek,Yongsik Jin,Dongyeop Kang,Soohee Han 제어로봇시스템학회 2020 제어로봇시스템학회 국제학술대회 논문집 Vol.2020 No.10
In this paper, robust H∞ explicit input and output feedback control (EIOC) is investigated for discrete-time linear parameter varying (LPV) systems. LPV systems with bounded parameters are represented as multiple polytopic linear systems. For the systems, EIOC is designed considering H∞ performance. EIOC employs additional signals of the past intput and output signals with output feedback control, so widen the capability of the closed-loop systems. The proposed design methodology takes advantages of additional parameter to improve robustness. The effectiveness and feasibility is shown through the numerical example.
Mitochondrial chaperone HSP ‐60 regulates anti‐bacterial immunity via p38 MAP kinase signaling
Jeong, Dae‐,Eun,Lee, Dongyeop,Hwang, Sun‐,Young,Lee, Yujin,Lee, Jee‐,Eun,Seo, Mihwa,Hwang, Wooseon,Seo, Keunhee,Hwang, Ara B,Artan, Murat,Son, Heehwa G,Jo, Jay‐,Hyun,Baek, Haes Published for the European Molecular Biology Organ 2017 The EMBO journal Vol.36 No.8
<P>Mitochondria play key roles in cellular immunity. How mitochondria contribute to organismal immunity remains poorly understood. Here, we show that HSP-60/HSPD1, a major mitochondrial chaperone, boosts anti-bacterial immunity through the up-regulation of p38 MAP kinase signaling. We first identify 16 evolutionarily conserved mitochondrial components that affect the immunity of Caenorhabditis elegans against pathogenic Pseudomonas aeruginosa (PA14). Among them, the mitochondrial chaperone HSP-60 is necessary and sufficient to increase resistance to PA14. We show that HSP-60 in the intestine and neurons is crucial for the resistance to PA14. We then find that p38 MAP kinase signaling, an evolutionarily conserved anti-bacterial immune pathway, is down-regulated by genetic inhibition of hsp-60, and up-regulated by increased expression of hsp-60. Overexpression of HSPD1, the mammalian ortholog of hsp-60, increases p38 MAP kinase activity in human cells, suggesting an evolutionarily conserved mechanism. Further, cytosol-localized HSP-60 physically binds and stabilizes SEK-1/MAP kinase kinase 3, which in turn up-regulates p38 MAP kinase and increases immunity. Our study suggests that mitochondrial chaperones protect host eukaryotes from pathogenic bacteria by up-regulating cytosolic p38 MAPK signaling.</P>