접착테이프 상에서의 패럴린 구조물로 이루어진 유연한 미세 유체 채널의 제작

이동희(Donghee Lee),김한별(Hanbyul Kim),성대경(Daekyung Sung),양성(Sung Yang) 대한기계학회 2013 대한기계학회 춘추학술대회 Vol.2013 No.12

가맹사업거래의 공정화에 관한 법률상 징벌적 손해배상에 대한 연구

양동희 ( Donghee Yang ),박승남 ( Seungnam Park ) 사단법인 아시아문화학술원 2021 인문사회 21 Vol.12 No.1

2011년 하도급법 제35조에 징벌적 손해배상제도가 도입된 후 개별 법률에 본 제도를 신설하였으나, 여전히 가해자의 불법행위로 다수피해자가 발생하고 있다. 사후적 전보배상 만으로는 가해자에 대한 충분한 처벌과 유사행위 억제를 담보하는데 한계가 있다는 비판에 따라 징벌적 손해배상 도입의 필요성이 제기되었다. 이에 법무부는 2020. 9. 28. 상법 제66조의2에 상인의 손해배상책임에 대한 특례를 신설한 상법 일부개정안을 제안하였다. 본 논문에서는 상법 개정안과 상법의 특별법인 가맹사업법 제37조의2 제2항을 비교하여 불공정거래행위의 근절과 과잉처벌 논란이 있는 징벌적 손해배상제도의 개정안을 제안한다. 이를 통해 가맹사업거래의 공정화에 관한 법률에 대한 관심과 가맹본부 및 가맹본부 임원 등의 고의적이고 악의적인 불법행위 억지, 실무계의 업무 부담을 덜 수 있기를 바란다. After the introduction of the punitive damages system in Article 35 of the Fair Transactions in Subcontracts Act in 2011, this system was newly established in individual laws, but many victims arise by the perpetrators’ illegal activities. The need for the introduction of punitive damages was proposed following criticism that ex-post compensation only has limitations in ensuring sufficient punishment for perpetrators and suppression of similar acts. In response, the Ministry of Justice proposed a partial amendment to a portion of the Commercial Act in particular Article 66-2 of the Commercial Act, which established a special case for merchants' liability for damages on September 28th, 2020. In this paper, we propose a revision to the punitive damages system, which is controversial because of the eradication of unfair trade practices and over-punishment by comparing the amendment to the Commercial Act and Article 37-2 (2) of the Fair Transactions in Franchise Business Act which is Special Act with Commercial Act. Through this, we hope to create interest in the law on the fairness of franchise business transactions, to deter deliberate and malicious illegal activities on the part of such things as affiliate headquarters and affiliate headquarters officers, and to lessen the burden of the working-level world.

On-Chip Parylene-C Microstencil for Simple-to-Use Patterning of Proteins and Cells on Polydimethylsiloxane

Lee, Donghee,Yang, Sung American Chemical Society 2013 ACS APPLIED MATERIALS & INTERFACES Vol.5 No.7

<P>Polydimethylsiloxane (PDMS) is widely used as a substrate in miniaturized devices, given its suitability for execution of biological and chemical assays. Here, we present a patterning approach for PDMS, which uses an on-chip Parylene-C microstencil to pattern proteins and cells. To implement the on-chip Parylene-C microstencil, we applied SiO<I>x</I>-like nanoparticle layers using atmospheric-pressure plasma-enhanced chemical vapor deposition (AP-PECVD) of tetraethyl orthosilicate (TEOS) mixed with oxygen. The complete removal of Parylene-C from PDMS following application of SiO<I>x</I>-like nanoparticle layers was demonstrated by various surface characterization analysis, including optical transparency, surface morphology, chemical composition, and peel-off force. Furthermore, the effects of the number of AP-PECVD treatments were investigated. Our approach overcomes the tendency of Parylene-C to peel off incompletely from PDMS, which has limited its use with PDMS to date. The on-chip Parylene-C microstencil approach that is based on this Parylene-C peel-off process on PDMS can pattern proteins with 2-μm resolution and cells at single-cell resolution with a vacancy ratio as small as 10%. This provides superior user-friendliness and a greater degree of geometrical freedom than previously described approaches that require meticulous care in handling of stencil. Thus, this patterning method could be applied in various research fields to pattern proteins or cells on the flexible PDMS substrate.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/aamick/2013/aamick.2013.5.issue-7/am4001166/production/images/medium/am-2013-001166_0012.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/am4001166'>ACS Electronic Supporting Info</A></P>

Reactive Oxygen Species Enhance TLR10 Expression in the Human Monocytic Cell Line THP-1

Kim, Donghee,Kim, Yeon Ju,Koh, Hyun Sook,Jang, Tae Yang,Park, Hyo Eun,Kim, Jae Young Molecular Diversity Preservation International (MD 2010 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.11 No.10

<P>We investigated TLR10 expression in human monocytes, THP-1 cells, cultured in hypoxia (3% O<SUB>2</SUB>). Levels of both TLR10 mRNA and protein in THP-1 cells cultured in hypoxia were significantly higher than those cultured in normoxia (20% O<SUB>2</SUB>). We examined intracellular reactive oxygen species (ROS) content in hypoxic cells, and TLR10 expression in cells treated with hydrogen peroxide (H<SUB>2</SUB>O<SUB>2</SUB>), to determine whether the increase in TLR10 expression observed with hypoxia was due to an increase in intracellular ROS levels. We found that the level of intracellular ROS in cells subject to hypoxia was significantly higher than in normoxia. Experiments with ROS synthesis inhibitors revealed that hypoxia induced ROS production is mainly due to NADPH oxidase activity. TLR10 mRNA expression was increased by treatment with H<SUB>2</SUB>O<SUB>2</SUB> at concentrations ranging from 50 to 250 μM. We screened the TLR10 promoter and found putative binding sites for transcription factors (TFs), such as NF-κB, NF-AT and AP-1. Next, we examined TF activities using a luciferase reporter assay. Activities of NF-κB, NF-AT and AP-1 in the cells treated with H<SUB>2</SUB>O<SUB>2</SUB> were significantly higher than in untreated cells. The experiment with TF inhibitors revealed that ROS-induced upregulation of TLR10 expression is mainly due to NF-κB activation. Overall, our results suggest that hypoxia or ROS increase TLR10 expression in human monocytes and the transcriptional activities of NF-κB are involved in this process. Therefore, it is suggested that ROS produced by various exogenous stimuli may play a crucial role in the regulation of expression and function of TLR10 as second messengers.</P>

Study of properties and toxicity of reduced Cr(III) combined with organic materials eluted from pine bark by biosorption

Hanui Yang,Namgyu Kim,Donghee Park 대한환경공학회 2019 대한환경공학회 학술발표논문집 Vol.2019 No.-

Bioresorbable Electronic Stent Integrated with Therapeutic Nanoparticles for Endovascular Diseases

Son, Donghee,Lee, Jongha,Lee, Dong Jun,Ghaffari, Roozbeh,Yun, Sumin,Kim, Seok Joo,Lee, Ji Eun,Cho, Hye Rim,Yoon, Soonho,Yang, Shixuan,Lee, Seunghyun,Qiao, Shutao,Ling, Daishun,Shin, Sanghun,Song, Jun- American Chemical Society 2015 ACS NANO Vol.9 No.6

<P>Implantable endovascular devices such as bare metal, drug eluting, and bioresorbable stents have transformed interventional care by providing continuous structural and mechanical support to many peripheral, neural, and coronary arteries affected by blockage. Although effective in achieving immediate restoration of blood flow, the long-term re-endothelialization and inflammation induced by mechanical stents are difficult to diagnose or treat. Here we present nanomaterial designs and integration strategies for the bioresorbable electronic stent with drug-infused functionalized nanoparticles to enable flow sensing, temperature monitoring, data storage, wireless power/data transmission, inflammation suppression, localized drug delivery, and hyperthermia therapy. <I>In vivo</I> and <I>ex vivo</I> animal experiments as well as <I>in vitro</I> cell studies demonstrate the previously unrecognized potential for bioresorbable electronic implants coupled with bioinert therapeutic nanoparticles in the endovascular system.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/ancac3/2015/ancac3.2015.9.issue-6/acsnano.5b00651/production/images/medium/nn-2015-00651k_0006.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/nn5b00651'>ACS Electronic Supporting Info</A></P>

고체추진 핀틀 추력기의 저주파 비음향 불안정 해석에 관한 연구

이동희(Donghee Lee),주성민(Seongmin Joo),김준성(Junseong Kim),류승현(Seunghyun Ryu),성홍계(Honggye Sung),문희장(Heejang Moon),양준서(Juneseo Yang) 한국추진공학회 2015 한국추진공학회 학술대회논문집 Vol.2015 No.11

핀틀 추력기는 노즐목 면적의 변화로 추력제어를 함으로써 자세제어 및 궤도천이가 가능한 고체로켓 시스템이다. 고체로켓 시스템은 고고도(high altitude)에서 낮은 압력으로 운영되며 이 경우 L<SUP>*</SUP> 불안정이 나타날 수 있다. 본 연구는 대표적인 저주파 비음향 불안정인 L<SUP>*</SUP> 불안정 이론을 고체추진 핀틀 추력기에 적용하여 연소실의 L<SUP>*</SUP> 안정도 판별에 대한 연구를 수행하였다. 핀틀 추력기의 설계 파라미터와 추진제 물성치를 이용하여 L<SUP>*</SUP> 안정도 판별 곡선을 도출하였고 핀틀 위치가 0 mm, 10 mm, 20 mm, 30 mm, 40 mm일 때 추력기의 L<SUP>*</SUP> 안정도를 판단하였다. 또한, 추력기의 L<SUP>*</SUP> 불안정 임계점을 파악하였으며 이에 따른 핀틀의 안정적인 구동 범위를 제시하였다. A study of L<SUP>*</SUP> instability, a common low-frequency non-acoustic instability, was investigated and applied to a solid propellant pintle thruster. Parameters of the thruster chamber and propellant are used to fraw the L<SUP>*</SUP> stability criteria curve, which will determine the L<SUP>*</SUP> stability at each position of the pintle(0 mm, 10 mm, 20 mm, 30 mm, 40 mm). Results show the critical point and proposes a stable operating range of the pintle.

Improvement Characteristics of Bio-active Materials Coated Fabric on Rat Muscular Mitochondria

Lee, Donghee,Kim, Young-Won,Kim, Jung-Ha,Yang, Misuk,Bae, Hyemi,Lim, Inja,Bang, Hyoweon,Go, Kyung-Chan,Yang, Gwang-Wung,Rho, Yong-Hwan,Park, Hyo-Suk,Park, Eun-Ho,Ko, Jae-Hong The Korean Society of Pharmacology 2015 The Korean Journal of Physiology & Pharmacology Vol.19 No.3

This study surveys the improvement characteristics in old-aged muscular mitochondria by bio-active materials coated fabric (BMCF). To observe the effects, the fabric (10 and 30%) was worn to old-aged rat then the oxygen consumption efficiency and copy numbers of mitochondria, and mRNA expression of apoptosis- and mitophagy-related genes were verified. By wearing the BMCF, the oxidative respiration significantly increased when using the 30% materials coated fabric. The mitochondrial DNA copy number significantly decreased and subsequently recovered in a dose-dependent manner. The respiratory control ratio to mitochondrial DNA copy number showed a dose-dependent increment. As times passed, Bax, caspase 9, PGC-$1{\alpha}$ and ${\beta}$-actin increased, and Bcl-2 decreased in a dose-dependent manner. However, the BMCF can be seen to have had no effect on Fas receptor. PINK1 expression did not change considerably and was inclined to decrease in control group, but the expression was down-regulated then subsequently increased with the use of the BMCF in a dose-dependent manner. Caspase 3 increased and subsequently decreased in a dose-dependent manner. These results suggest that the BMCF invigorates mitophagy and improves mitochondrial oxidative respiration in skeletal muscle, and in early stage of apoptosis induced by the BMCF is not related to extrinsic death-receptor mediated but mitochondria-mediated signaling pathway.

Spherical Macroporous Carbon Nanotube Particles with Ultrahigh Sulfur Loading for Lithium–Sulfur Battery Cathodes

Gueon, Donghee,Hwang, Jeong Tae,Yang, Seung Bo,Cho, Eunkyung,Sohn, Kwonnam,Yang, Doo-Kyung,Moon, Jun Hyuk American Chemical Society 2018 ACS NANO Vol.12 No.1

<P>A carbon host capable of effective and uniform sulfur loading is the key for lithium–sulfur batteries (LSBs). Despite the application of porous carbon materials of various morphologies, the carbon hosts capable of uniformly impregnating highly active sulfur is still challenging. To address this issue, we demonstrate a hierarchical pore-structured CNT particle host containing spherical macropores of several hundred nanometers. The macropore CNT particles (M-CNTPs) are prepared by drying the aerosol droplets in which CNTs and polymer particles are dispersed. The spherical macropore greatly improves the penetration of sulfur into the carbon host in the melt diffusion of sulfur. In addition, the formation of macropores greatly develops the volume of the micropore between CNT strands. As a result, we uniformly impregnate 70 wt % sulfur without sulfur residue. The S-M-CNTP cathode shows a highly reversible capacity of 1343 mA h g<SUP>–1</SUP> at a current density of 0.2 C even at a high sulfur content of 70 wt %. Upon a 10-fold current density increase, a high capacity retention of 74% is observed. These cathodes have a higher sulfur content than those of conventional CNT hosts but nevertheless exhibit excellent performance. Our CNTPs and pore control technology will advance the commercialization of CNT hosts for LSBs.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/ancac3/2018/ancac3.2018.12.issue-1/acsnano.7b05869/production/images/medium/nn-2017-05869j_0007.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/nn7b05869'>ACS Electronic Supporting Info</A></P>

0.1 MWth 가압 순산소 연소 시스템의 연소 및 NOx 배출 특성 연구

김동희(Donghee Kim),박진제(Jinje Park),양원(Won Yang),허강열(Kang Y. Huh),이영재(Youngjae Lee) 한국연소학회 2020 KOSCOSYMPOSIUM논문집 Vol.2020 No.9