MicroRNA expression profiles in conventional and micropropagated Dendrobium officinale

Zhili Yang,Dongfeng Yang,Xianfeng Ding,Yong Gao,Donghai Li,Tao Xu 한국유전학회 2015 Genes & Genomics Vol.37 No.4

MicroRNA (miRNA) is defined as an endogenous *22 nt noncoding RNA that has critical gene-regulatory functions in plants. Dendrobium officinale is among the most important herbs in traditional Chinese medicine. However, no miRNA of D. officinale has been reported to date. To identify the miRNA in D. officinale and to detect the differentially expressed miRNA between conventional and micropropagated D. officinale plants, miRNA microarray was performed following quantitative real-time RTPCR validation. A total of 120 miRNAs from 37 miRNA families were identified in both conventional and micropropagated D. officinale plants. Among the total number of miRNAs, 45 miRNAs from six families were differentially expressed, including five up-regulated families (miR156, miR164, miR171, miR827 and miR529) and one downregulated family (miR167) in micropropagated group. Web microrna designer and gene ontology term-enrichment analyses were applied for prediction and functional analysis of the target genes of differentially expressed miRNA. Such miRNAs are mainly related to hormone stimulus response, biological and metabolic process regulation, formation of intracellular organelle, and nucleic acid binding.

The Role of Gut Microbiota and Genetic Susceptibility in the Pathogenesis of Pancreatitis

Xu Fumin,Yang Chunmei,Tang Mingcheng,Wang Ming,Cheng Zhenhao,Chen Dongfeng,Chen Xiao,Liu Kaijun 거트앤리버 소화기연관학회협의회 2022 Gut and Liver Vol.16 No.5

Pancreatitis is one of the most common inflammatory diseases of the pancreas caused by autodigestion induced by excessive premature protease activation. However, recognition of novel pathophysiological mechanisms remains a still challenge. Both genetic and environmental factors contribute to the pathogenesis of pancreatitis, and the gut microbiota is a potential source of an environmental effect. In recent years, several new frontiers in gut microbiota and genetic risk assessment research have emerged and improved the understanding of the disease. These investigations showed that the disease progression of pancreatitis could be regulated by the gut microbiome, either through a translocation influence or in a host immune response manner. Meanwhile, the onset of the disease is also associated with the heritage of a pathogenic mutation, and the disease progression could be modified by genetic risk factors. In this review, we focused on the recent advances in the role of gut microbiota in the pathogenesis of pancreatitis, and the genetic susceptibility in pancreatitis.

Cross-Layer Cooperative Scheduling Scheme for Multi-channel Hybrid Ubiquitous Sensor Networks

Yingji Zhong,Qinghai Yang,곽경섭,Dongfeng Yuan 한국전자통신연구원 2008 ETRI Journal Vol.30 No.5

The multi-scenario topology of multi-channel hybrid ubiquitous sensor networks (USNs) is studied and a novel link auto-diversity cross-layer cooperative scheduling scheme is proposed in this paper. The proposed scheme integrates the attributes of the new performance evaluation link auto-diversity air-time metric and the topology space in the given multi-scenario. The proposed scheme is compared with other schemes, and its superiority is demonstrated through simulations. The simulation results show that relative energy consumption, link reception probability, and end-to-end blocking probability are improved. The addressing ratio of success with unchanged parameters and external information can be increased. The network can tolerate more hops to support reliable transportation when the proposed scheme is implemented. Moreover, the scheme can make the network stable. Therefore, the proposed scheme can enhance the average rate performance of the hybrid USN and stabilize the outage probability.

High-Frequency Isolated Medium-Voltage AC Grid-connected PV Power Generation System Structure based on DC Bus Collection

Lin. Lin,Qi. Chen,Xingang. Wu,Chuang. Liu,Haoran. Zhang,Dongfeng. Yang 전력전자학회 2019 ICPE(ISPE)논문집 Vol.2019 No.5

This paper proposes an isolated mediumvoltage AC grid-connected PV power generation system structure based on DC bus collection with high-frequency link concept. The proposed system structure consists of centralized photovoltaic power plant, inverter unit based on isolated bridge converter (I-BC), and capacitor at the junction of dc bus. PV plant is divided into many arrays, each PV array is connected in parallel with each phase inverter unit. Inverter unit is composed of two I-BC. Compare with conventional two-stage PV inverter, I-BC can totally eliminate the individual DC-link capacitors at the highvoltage side, which leads to extremely reduced system volume and voltage-balancing control between individual capacitors. Second harmonic power oscillations seen in typical system structure can also be eliminated or reduced through use of the proposed system. This paper presents the analysis and operation of the proposed PV system structure with experimental results on a scaled-down laboratory.

Investigation of the optimal fabrication of a single-carrier encapsulated fucoxanthin based on colloidal nanoparticles

Xin Zhang,Minghao Fan,Yongkai Yuan,Jianjun Dong,Hua Yin,Yang He,Lei Mao,Dongfeng Wang,Junhong Yu 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.114 No.-

Fucoxanthin (FX) is a carotenoid with antioxidant, anti-obesity, anti-diabetic, anti-cancer and antibacterialactivities. It is poorly water soluble and highly sensitive to light, heat and the surrounding environment. Therefore, how to effectively encapsulate it and stabilize it for entry into the human body hasbecome a key research question at present. This study developed a single-carrier to encapsulate FX andshowed excellent characterization results. The experimental results showed that the diameters of FX-ZHparticles prepared by the four encapsulation processes ranged from 143.83 to 216.93 nm. Comparison ofantioxidant activity and stability to temperature and pH of the four complexes showed significant differences,with the best performance of the nanoparticles prepared using water-soluble method and a slowspeedstirrer. The nanocomplexes were proved to be more stable and more bioavailable, with a significant34.41% increase in FX content relative to free FX in the intestinal phase. Inhibition of human leukaemiacells HL-60 cells remained high, with 11.14% ± 6.03% inhibition at FX concentrations of 1 lg/mL. Thisstudy encapsulated FX based on colloidal nanoparticle systems for the first time using a single-carriermaterial, an innovation and breakthrough that could simplify the experimental steps and provide thenecessary basis for industrial realization.

Genome-Wide Association Study Meta-Analysis Reveals Transethnic Replication of Mean Arterial and Pulse Pressure Loci

Kelly, Tanika N.,Takeuchi, Fumihiko,Tabara, Yasuharu,Edwards, Todd L.,Kim, Young Jin,Chen, Peng,Li, Huaixing,Wu, Ying,Yang, Chi-Fan,Zhang, Yonghong,Gu, Dongfeng,Katsuya, Tomohiro,Ohkubo, Takayoshi,Gao American Heart Association, Inc. 2013 Hypertension Vol.62 No.5

<P>We conducted a genome-wide association study meta-analysis of mean arterial pressure and pulse pressure among 26 600 East Asian participants (stage 1) followed by replication study of up to 28 783 participants (stage 2). For novel loci, statistical significance was determined by a <I>P</I><5.0×10<SUP>–8</SUP> in joint analysis of stage 1 and stage 2 data. For loci reported by the previous mean arterial and pulse pressure genome-wide association study meta-analysis in Europeans, evidence of transethnic replication was determined by consistency in effect direction and a Bonferroni-corrected <I>P</I><1.4×10<SUP>–3</SUP>. No novel loci were identified by the current study. Five independent mean arterial pressure variants demonstrated robust evidence for transethnic replication including rs17249754 at <I>ATP2B1</I> (<I>P</I>=7.5×10<SUP>–15</SUP>), rs2681492 at <I>ATP2B1</I> (<I>P</I>=3.4×10<SUP>–7</SUP>), rs11191593 at <I>NT5C2</I> (1.1×10<SUP>–6</SUP>), rs3824755 at <I>CYP17A1</I> (<I>P</I>=1.2×10<SUP>–6</SUP>), and rs13149993 at <I>FGF5</I> (<I>P</I>=2.4×10<SUP>–4</SUP>). Two additional variants showed suggestive evidence of transethnic replication (consistency in effect direction and <I>P</I><0.05), including rs319690 at <I>MAP4</I> (<I>P</I>=0.014) and rs1173771 at <I>NPR3</I> (<I>P</I>=0.018). For pulse pressure, robust evidence of replication was identified for 2 independent variants, including rs17249754 at <I>ATP2B1</I> (<I>P</I>=1.2×10<SUP>–5</SUP>) and rs11191593 at <I>NT5C2</I> (<I>P</I>=1.1×10<SUP>–3</SUP>), with suggestive evidence of replication among an additional 2 variants including rs3824755 at <I>CYP17A1</I> (<I>P</I>=6.1×10<SUP>–3</SUP>) and rs2681492 at <I>ATP2B1</I> (<I>P</I>=9.0×10<SUP>–3</SUP>). Replicated variants demonstrated consistency in effect sizes between East Asian and European samples, with effect size differences ranging from 0.03 to 0.24 mm Hg for mean arterial pressure and from 0.03 to 0.21 mm Hg for pulse pressure. In conclusion, we present the first evidence of transethnic replication of several mean arterial and pulse pressure loci in an East Asian population.</P>