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Construction of composite comparative genetic maps for ten legume species
Dongwoon Yu,Chaeyoung Lee,Daejin Hyung,Jinhyun Kim,Yejin Jo,Jusuk Park,Eunji Kwon,Hongkyu Choi 한국육종학회 2013 한국육종학회 심포지엄 Vol.2013 No.07
The legume family is the third largest group, including approximately 650 genera and 18,000 species, in the flowering plants and the second important crops to the Poaceae in the agricultural economy. Comparative analysis is a useful tool to understand cross-species genomic structure and alterations during organism’s evolutionary history. In this study, we constructed a composite comparative map of ten legume species, including Medicago truncatula, Medicago sativa, Lens culinaris, Pisum sativum, Lotus japonicus, Cicer arietinum, Vicia faba L, Vigna radiata, Phaseolus vulgaris and Glycine max. Of these species, M. truncatula, which is a representative model system, played a central role to develop the cross-genome amplifiable PCR gene markers for the purpose of transferring them to other related legume species. A total of 108 cross-species core markers were employed to analyze genomic colinearity across this broad array of legume species. The comparative map demonstrates a diverse array of evolutionary events, such as duplications, inversions and reciprocal translocations. It is anticipated that resulting maps would provide a broader insights into the lineage-specific genomic organization of these glalegoid/phaseoloid legumes, which are two clades containing almost all crop legumes of economic importance, and can further used for the molecular breeding through translating genomic information into other orphan legumes.
Development of 5 D.O.F Robot Hand for an Android robot
Dongwoon Che,HyunSub Park,Dong-Wook Lee,Jun-Young Jung,Ho-Gil Lee 제어로봇시스템학회 2009 제어로봇시스템학회 국제학술대회 논문집 Vol.2009 No.8
In this paper, we propose 5 D.O.F robot hand for an android robot. A robot hand is the most complicated part in robot and it required many functions like grasping, detailed motion and so on. An android robot is one of humanoid robot but it required more similar appearance than one of humanoid robot thus, a hand of an android robot is required human like shape more than a hand of humanoid robot. The hand of android needs human like shape and complication, therefore it is difficult to develop a hand of an android robot. In this research, we develop a hand of an android robot which has 5 D.O.F fingers, human like shape and skin. Each finger has link structure driven by DC motor and worm gear. The finger is designed independent module structure so, hand is only combination of finger modules. This module structure gives small size, easy modification and convenience of maintenance. The skin is made of silicon complex material and it designed based on real human hand. The size of this hand based on standard size of Korean woman.
Cyst Nematode: A big problem that’s getting bigger in Korea
DongWoon Lee,Md. Faisal Kabir,Mun Gi Chung 한국응용곤충학회 2017 한국응용곤충학회 학술대회논문집 Vol.2017 No.10
Cyst nematodes are well known to cause serious yield losses in many important crops producing in Korea such assoybeans and Chinese cabbage. Recently, a new cyst nematode Heterodera trifolii has been recorded in the highland vegetablesgrowing area occurring severe yield losses. As a newly recorded pest, the severity of the nematode is still not clear tomost of the farmers. Identification of this nematode is another big issue as the nematode existed in the field with Heteroderaschachtii. Recent reports revealed that the pest is steadily spreading within the highland areas and has a huge possibilityto spread over Korea. Therefore, it is the high time to set a monitoring or warning systems and test more feasible managementstrategies to prevent the dispersal of this nematode.
Chaeyoung Lee,Daejin Hyung,Jinhyun Kim,Dongwoon Yu,Jeonghoon Park,Meejin Kim,Hyun-A So,Eunsook Jeong,Howon Chung,Youngsoo Chung,Dohoon Kim,Jaihun Lee,Hongkyu Choi 한국육종학회 2012 한국육종학회 심포지엄 Vol.2012 No.07
For purposes of studying intron structures and predicting consensus splice motifs, a total of 102 legume species were used to isolate introns across the family. Of 196 gene-targeted PCR primer pairs, we successfully amplified 118 intron-containing genes (60.2%) and obtained a total of 1,870 introns with an average size of 143 nucleotides. Species-based compilation of 5’- and 3’-splicing motifs showed lineage-specific conservation in each splicing motif. Compilation of the entire intron set permitted prediction of the consensus sequences of splicing signal motifs in legumes, AYGWGTABABGH and TVNC/TAGGHTV for the 5’- and 3’-splicing motifs, respectively. Interestingly, these consensus motifs are very similar to the corresponding splicing signals of two model systems, Arabidopsis and rice. This result is suggestive of conservation of pre-mRNA splicing mechanisms in higher plants. Multiple alignments of CALTL introns demonstrated that the region from the branch point to 3’ splice site was relatively more conserved than the region from5’ splice site to the branch point. Phylogenetic analysis demonstrated that each of three splicing motifs, 5’-splice sites, 3’-splice sits, and branch site, was relevant to evolutionary divergence of species and phylogenetically informative, suggesting that splice signal sequences would be useful as a potential tool for the molecular phylogenetic analysis.