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Biswas, Dibyendu,So, Kyoung Ha,Hwang, Seon Ung,Yoon, Junchul David,Kim, Mirae,Kim, Dae Young,Hyun, Sang Hwan Elsevier 2018 Theriogenology Vol.120 No.-
<P><B>Abstract</B></P> <P>Current research suggests that supplementing <I>in vitro</I> culture (IVC) media with vascular endothelial growth factor (VEGF) may have beneficial effects on the development of porcine embryos <I>in vitro</I>. However, the molecular signaling mechanisms underlying this effect are unclear. Therefore, we aimed to investigate the effects of VEGF on molecular signaling events during <I>in vitro</I> embryonic development of porcine embryos. Porcine oocytes matured <I>in vitro</I> were fertilized, and the resultant zygotes were cultured with 5 ng/mL of VEGF supplemented with or without fetal bovine serum from day 4 till day 7. Without VEGF and/or FBS served as the control group. Real-time quantitative PCR was used to detect expression patterns of apoptosis- and oxidative stress-related genes in day 7 blastocysts (BLs). Early-stage apoptosis was detected by annexin-V assays in day 2 and day 7 embryos. We found that the addition of VEGF throughout the culture period with or without FBS supplementation significantly improved embryo survival and development. Supplementation with VEGF in the IVC medium significantly increased early BL formation (p < 0.05), although addition of FBS on day 4 significantly increased hatched BL formation (p < 0.05) regardless of VEGF supplementation. However, supplementation of media with both VEGF and FBS increased the formation of expanded BLs synergistically. The average total cell numbers per BL were significantly (p < 0.05) higher in embryos supplemented with VEGF and FBS than in those supplemented with either VEGF or FBS alone. We also found that accumulation of reactive oxygen species in VEGF-treated embryos was significantly lower (p < 0.05) than that in untreated embryos. The mRNA levels of caspase-3 were significantly lower (p < 0.05), and those of Bcl<I>-</I>2 and Nrf<I>-</I>2 were significantly higher (p < 0.05) in embryos grown in VEGF-supplemented media than in embryos grown in non-supplemented media. Furthermore, on day 2, the numbers of viable embryos (44.06 ± 3.94%) and blastomeres (67.18 ± 3.60%) were significantly higher (p < 0.05), and the numbers of early apoptotic embryos (55.94 ± 3.94) and blastomeres (23.23 ± 4.22) were significantly lower (p < 0.05) in VEGF-treated BLs than in controls. Furthermore, the numbers of early apoptotic cells in BLs on day 7 were also significantly lower (p < 0.05) in VEGF-treated BLs than in controls. Overall, our results indicate that supplementing IVC media with VEGF during <I>in vitro</I> culture of porcine embryos increases their developmental potential.</P>
Effect of Vascular Endothelial Growth Factor on Porcine In Vitro Maturation
Dibyendu Biswas,현상환 사단법인 한국동물생명공학회 2007 한국동물생명공학회지 Vol.22 No.4
This study was performed to investigate the effect of VEGF on in vitro maturation of porcine oocytes. The base medium for IVM, TCM-199 was supplemented with 0.6 mM cysteine, 0.91 mM pyruvate, 10 ng/ml epidermal growth factor, 75 μg/ ml kenamycin, 1 μg/ml insulin and 10% (V/V) porcine follicular fluid (pFF) as a Group A; Group B was consists of Group A plus 5 ng/ml VEGF; Group C was consists of replacement of pFF by 10% PVA and Group D: was consists of Group C plus 5 ng/ml VEGF. 1. The maturation rate was significantly higher (p<0.05) in control and VEGF+pFF group than other two groups (76.1± 9.6, 78.9±6.0 vs 60.4±14.2 and 58.3±14.3, respectively). 2. Addition of VEGF without pFF showed a negative effect on oocyte maturation and about 58.26% oocytes were reached to M-Ⅱ stage. 3. In the parthenogenetic development, the cleavage rate was significantly higher (p<0.05) in control and VEGF+ pFF group (73.2±1.8 and 64.6±1.1, respectively) than other groups (47.9± 1.8 and 48.3±1.7, respectively). 4. The blastocyst formation rate was significantly higher (p<0.05) in VEGF+pFF group (32.6±2.4) compared to control and other groups.5. There was no significant difference in cell numbers (inner cell mass or trophectoderm) among these groups.
Dibyendu Biswas,Zahed Md.Malekur Rahman 강원대학교 산림과학연구소 2018 Journal of Forest Science Vol.34 No.1
Rabies causes the highest mortality of all viral diseases in the world unless the victim has been protected either by active immunization or post-exposure immunoprophylaxis. Infected stray dogs, raccoons, skunks, foxes and bats are the demonstrated carriers of most cases of rabies. It is difficult to diagnose a rabid animal in the field unless characteristic clinical signs are evident. However, this study used a commercial fast check kit comprised of immunochromatographic test (ICT) strips (ICTS) to diagnose rabies infection in clinically suspected samples obtained from a wildebeest. A 10-year old male wildebeest (approximate weight, 150 kg) died at Bangabandhu Sheikh Mujib Safari (BSMS) Park, Cox’s Bazar, Bangladesh with a clinical history of severe excitation and abundant oral secretions. A gross pathological examination revealed no specific lesions indicating any fatal diseases. The entire brain was collected within 6 hours of death, and the brain sample was tested using the ICT strips following the manufacturer’s directions. The rabies viral antibody was detected within the brain stem and medulla of the brain tissue of the dead wildebeest.
Effect of Vascular Endothelial Growth Factor on Porcine In Vitro Maturation
Biswas, Dibyendu,Hyun, Sang-Hwan 韓國受精卵移植學會 2007 한국동물생명공학회지 Vol.22 No.4
This study was performed to investigate the effect of VEGF on in vitro maturation of porcine oocytes. The base medium for IVM, TCM-199 was supplemented with 0.6 mM cysteine, 0.91 mM pyruvate, 10 ng/ml epidermal growth factor, kenamycin, insulin and 10% (V/V) porcine follicular fluid (pFF) as a Group A; Group B was consists of Group A plus 5 ng/ml VEGF; Group C was consists of replacement of pFF by 10% PVA and Group D: was consists of Group C plus 5 ng/ml VEGF. 1. The maturation rate was significantly higher (p<0.05) in control and VEGF+pFF group than other two groups (, respectively). 2. Addition of VEGF without pFF showed a negative effect on oocytes maturation and about 58.26% oocytes were reached to M-II stage. 3. In the parthenogenetic development, the cleavage rate was significantly higher (p<0.05) in control and VEGF+pFF group (, respectively) than other groups (, respectively). 4. The blastocyst formation rate was significantly higher (p<0.05) in VEGF+pFF group () compared to control and other groups. 5. There was no significant difference in cell numbers (inner cell mass or trophectoderm) among these groups.
Biswas, Dibyendu,Rahman, Zahed Md.Malekur Institute of Forest Science 2018 Journal of Forest Science Vol.34 No.1
Rabies causes the highest mortality of all viral diseases in the world unless the victim has been protected either by active immunization or post-exposure immunoprophylaxis. Infected stray dogs, raccoons, skunks, foxes and bats are the demonstrated carriers of most cases of rabies. It is difficult to diagnose a rabid animal in the field unless characteristic clinical signs are evident. However, this study used a commercial fast check kit comprised of immunochromatographic test (ICT) strips (ICTS) to diagnose rabies infection in clinically suspected samples obtained from a wildebeest. A 10-year old male wildebeest (approximate weight, 150 kg) died at Bangabandhu Sheikh Mujib Safari (BSMS) Park, Cox's Bazar, Bangladesh with a clinical history of severe excitation and abundant oral secretions. A gross pathological examination revealed no specific lesions indicating any fatal diseases. The entire brain was collected within 6 hours of death, and the brain sample was tested using the ICT strips following the manufacturer's directions. The rabies viral antibody was detected within the brain stem and medulla of the brain tissue of the dead wildebeest.
Polar Body: Indicator of Oocyte's Maturation, Have Any Function on Oocyte?
Dibyendu, Biswas,Hyun, Sang-Hwan 韓國受精卵移植學會 2009 한국동물생명공학회지 Vol.24 No.4
Polar body was usually used as a determinant of oocyte's maturation. Polar body morphology could reflect the embryo quality and implantation competence. This review only focuses on morphology of the first polar body and embryo developmental rate in the presence or absence of polar body. However, it is very difficult to describe whether polar body has any effects on embryo development in vitro or in vivo. Further intensive research is needed to determine its function on embryo development.