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- 검색키워드
- 저자 : Di-Hua Chen (검색결과 6 건)
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Chen, Peng,Wang, Xiu-Li,Ma, Zhong-Sen,Xu, Zhong,Jia, Bo,Ren, Jin,Hu, Yu-Xin,Zhang, Qing-Hua,Ma, Tian-Gang,Yan, Bing-Di,Yan, Qing-Zhu,Li, Yan-Lei,Li, Zhen,Yu, Jin-Yan,Gao, Rong,Fan, Na,Li, Bo,Yang, Jun Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.7
HMGN5 is a typical member of the HMGN (high mobility group nucleosome-binding protein) family which may function as a nucleosomal binding and transcriptional activating protein. Overexpression of HMGN5 has been observed in several human tumors but its role in tumorigenesis has not been fully clarified. To investigate its significance for human lung cancer progression, we successfully constructed a shRNA expression lentiviral vector in which sense and antisense sequences targeting the human HMGN5 were linked with a 9-nucleotide loop. Inhibitory effects of siRNA on endogenous HMGN5 gene expression and protein synthesis were demonstrated via real-time RT-PCR and western blotting. We found HMGN5 silencing to significantly inhibit A549 and H1299 cell proliferation assessed by MTT, BrdU incorporation and colony formation assays. Furthermore, flow cytometry analysis showed that specific knockdown of HMGN5 slowed down the cell cycle at the G0/G1 phase and decreased the populations of A549 and H1299 cells at the S and G2/M phases. Taken together, these results suggest that HMGN5 is directly involved in regulation cell proliferation in A549 and H1299 cells by influencing signaling pathways involved in cell cycle progression. Thus, our finding suggests that targeting HMGN5 may be an effective strategy for human lung cancer treatment.
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Fabrication of nonlinear optical films based on methacrylate/silica hybrid matrix
Di Wang,Xiaodong Chen,Xingwen Zhang,Wunjun Wang,Yunlong Liu,Lijiang Hua 한국물리학회 2009 Current Applied Physics Vol.9 No.3
A free-radical polymerization was performed to fabricate nonlinear optical (NLO) hybrid films based on nanostructural building blocks and an interpenetrating methacrylate/silica hybrid matrix (IHM). A bulky chromophore (disperse orange 3) bonded with ally glycidyl ether (AGE) was incorporated into the hybrid matrix in combination with (γ-methacrylpropyl)-silsesquioxane through the polymerization. Different amounts of polyhedral oligomeric silsesquioxane as modifiers were added into the IHM to enhance properties. It has been shown that the IHM increases the crosslink density of the network and the interaction potential between dipoles to help maintain the orientational order, and the generated NLO materials enhance the Tg, nonresonant second-order nonlinearity and temporal stability. A free-radical polymerization was performed to fabricate nonlinear optical (NLO) hybrid films based on nanostructural building blocks and an interpenetrating methacrylate/silica hybrid matrix (IHM). A bulky chromophore (disperse orange 3) bonded with ally glycidyl ether (AGE) was incorporated into the hybrid matrix in combination with (γ-methacrylpropyl)-silsesquioxane through the polymerization. Different amounts of polyhedral oligomeric silsesquioxane as modifiers were added into the IHM to enhance properties. It has been shown that the IHM increases the crosslink density of the network and the interaction potential between dipoles to help maintain the orientational order, and the generated NLO materials enhance the Tg, nonresonant second-order nonlinearity and temporal stability.
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Di Xu,Xiaoli Wu,Bo Li,Peng Li,Xing Ming,Tingtao Chen,Hua Wei,Feng Xu 한국식품과학회 2013 Food Science and Biotechnology Vol.22 No.2
Campylobacter jejuni is a worldwide foodborne pathogen recognized as a leading cause of human gastrointestinal enteritis. A rapid, sensitive, and specific method is required to monitor food and water in cases of contamination by this pathogen. This report presents a novel immunochromatographic test (ICT) using fluorescent microspheres labeled with polyclonal antibodies of C. jejuni as the capture reagent dispensed onto the conjugate pad. Polyclonal antibodies against the outer membrane protein PEB1 of C. jejuni were used as the detective reagent at the test line, whereas the goat anti-rabbit IgG was used on the control line. PEB1 was obtained by gene cloning and expression to prepare its antibody. In this study, a simple and rapid ICT is reported for detecting C. jejuni for the first time with a detection limit of 106 CFU/mL.
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Peptidoglycans Promotes Human Leukemic THP-1 Cell Apoptosis and Differentiation
Wang, Di,Xiao, Pei-Ling,Duan, Hua-Xin,Zhou, Ming,Liu, Jin,Li, Wei,Luo, Ke-Lin,Chen, Jian-Jun,Hu, Jin-Yue Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.12
The innate immune system coordinates the inflammatory response to pathogens. To do so, its cells must discriminate self from non-self utilizing receptors that identify molecules synthesized exclusively by microbes. Toll-like receptors have a crucial role in the detection of microbial infection in mammals and insects. In mammals, they have evolved to recognize conserved products unique to microbial metabolism. These include lipopolysaccharide (LPS), lipotechoic acids, and peptidoglycans (PGN). We show here that TLRs, including TLR2, are expressed on the THP-1 human leukemia cell line. Activation of TLR2 signaling in THP-1 by PGN induces the synthesis of various soluble factors and proteins including interleukin-$1{\beta}$, interleukin-8 and TNF-${\alpha}$ and apoptosis of THP-1 with PGN dose and time dependence. Moreover, in this study we show that PGN induces apoptosis of THP-1 cells in a TNF-${\alpha}$-dependent manner. These findings indicate that TLR2 signaling results in a cascade leading to tumor apoptosis and differentiation, which may suggest new clinical prospects using TLR2 agonists as cytotoxic agents in certain cancers.
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Rui-Le Pan,Di-Hua Chen,Jian-Yong Si,Xiao-Hong Zhao,Zhan Li,Li Cao 대한약학회 2009 Archives of Pharmacal Research Vol.32 No.2
Two new cyclolanostane diglycosides, cimifoetiside A (1) and cimifoetiside B (2), were isolated from an 80% ethanolic extract of the aerial part of Cimicifuga foetida L. (Ranuculaceae). Using spectral data and chemical analysis, the structures of 1 and 2 were identified as (23R, 24S) cimicigenol 3-O-β-D-glucopyranosyl-(1"→3')-β-D-xylopyranoside and (23R, 24S) cimicigenol 3-O-β-D-glucopyranosyl-(1"→2')-β-D-xylopyranoside, respectively. The in vitro immunosuppressive effects of the two new compounds 1 and 2, as well as four other known cyclolanostane saponins 3-6 on T cells were evaluated. All the agents tested effectively inhibited the proliferation of murine splenocytes induced by Concanavalin A (ConA), with IC50 values ranging from 12.7 nM to 33.3 nM.
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Hydrogen sulfide inhibits the growth of Escherichia coli through oxidative damage
Liu-Hui Fu,Zeng-Zheng Wei,Kang-Di Hu,Lan-Ying Hu,Yan-Hong Li,Xiao-Yan Chen,Zhuo Han,Gai-Fang Yao,Hua Zhang 한국미생물학회 2018 The journal of microbiology Vol.56 No.4
Many studies have shown that hydrogen sulfide (H2S) is both detrimental and beneficial to animals and plants, whereas its effect on bacteria is not fully understood. Here, we report that H2S, released by sodium hydrosulfide (NaHS), significantly inhibits the growth of Escherichia coli in a dose-dependent manner. Further studies have shown that H2S treatment stimulates the production of reactive oxygen species (ROS) and decreases glutathione (GSH) levels in E. coli, resulting in lipid peroxidation and DNA damage. H2S also inhibits the antioxidative enzyme activities of superoxide dismutase (SOD), catalase (CAT) and glutathione reductase (GR) and induces the response of the SoxRS and OxyR regulons in E. coli. Moreover, pretreatment with the antioxidant ascorbic acid (AsA) could effectively prevent H2S-induced toxicity in E. coli. Taken together, our results indicate that H2S exhibits an antibacterial effect on E. coli through oxidative damage and suggest a possible application for H2S in water and food processing.
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