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Dananjaya, S.H.S.,Udayangani, R.M.C.,Shin, S.Y.,Edussuriya, M.,Nikapitiya, C.,Lee, J.,De Zoysa, M. G. Fischer 2017 MICROBIOLOGICAL RESEARCH Vol.201 No.-
<P>Fusarium oxysporum is an ascomycete facultative fungus which generally affects to plants. However, it is recently known as a serious emerging opportunistic pathogen of human and other animals. F. oxysporum shows broad resistance to commonly used antifungal agents and therefore development of alternative therapeutic agents is required. In this study, we investigated the antifungal efficacy of plant based natural lawsone against pathogenic F. oxysporum. Antifungal susceptibility test determined the concentration dependent growth inhibition of lawsone against F. oxysporum with minimum inhibitory concentration (MIC) at 100 mu g/mL. Ultra-structural analysis indicates the prominent damage on cell wall of the mycelium after lawsone treatment, and suggests that it could increase the membrane permeability and disintegration of cells leading to cellular death. Propidium iodide (PI) uptake assay results showed the higher level of cell death in lawsone treated F. oxysporum which further confirms the loss of plasma membrane integrity. Also, detection of reactive oxygen species (ROS) using DCFH-DA has clearly indicated that lawsone (100 mu g/mL) can induce the ROS level in the filaments of F. oxysporum. MTT assay results showed the loss of viability and germination capacity of F. oxysporum spores by lawsone in concentration dependent manner. Moreover, lawsone treatment induced the mRNA expression of two autophagy related genes (ATG1 and ATG8) indicating that lawsone may activate the autophagy related pathways in F. oxysporum due to the oxidative stress generated by ROS. F. oxysporum infected zebrafish has recovered after lawsone therapy as a topical treatment suggesting that lawsone is a potential natural antifusariosis agent.</P>
Dananjaya, S.H.S.,Kumar, R. Saravana,Yang, Minyang,Nikapitiya, Chamilani,Lee, Jehee,De Zoysa, Mahanama Elsevier 2018 International journal of biological macromolecules Vol.108 No.-
<P><B>Abstract</B></P> <P>In this study, we investigated the antifungal activity and cytotoxicity of ZnO-chitosan nanocomposites (ZnO-C NCs) against <I>Candida albicans</I> and human epithelial type 2 (HEp2) cells, respectively. The crystalline phase, morphology, composition, particle size and optical absorption properties of the synthesized ZnO-C NCs were systematically investigated by various contemporary methods. The X-ray diffraction analysis results showed characteristic diffraction peaks corresponding to both ZnO and chitosan, while field-emission scanning electron microscopy (FESEM) displayed clusters of spherical shaped particulate morphology. UV–vis absorption spectra showed a shift in the optical absorption towards lower wavelength for ZnO-C NCs when compared to ZnO nanoparticles (NPs). The antifungal activity results (against <I>C. albicans</I>) showed that the minimum inhibitory concentration of ZnO NPs and ZnO-C NCs were 200μg/mL and 75μg/mL, respectively, suggesting the greater therapeutic potential of ZnO-C NCs. FESEM analysis results showed the substantial change in the external morphology of <I>C. albicans</I> after treatment with both ZnO NPs and ZnO-C NCs due to the fungal cell membrane damage. ZnO-C NCs displayed lower cytotoxicity with HEp2 cells indicating the good cytocompatibility of the synthesized ZnO-C NCs. It is expected that ZnO and chitosan complement each other and exhibit synergistic effects potential for antimicrobial and biomedical applications.</P> <P><B>Highlights</B></P> <P> <UL> <LI> ZnO NPs combined with low molecular weight chitosan to form ZnO- C NCs. </LI> <LI> ZnO-C NCs showed remarkable anti-candida activity compared to ZnO NPs. </LI> <LI> ZnO-C NCs showed negligible sensitivity to HEp2 cells. </LI> <LI> ZnO-C NCs could be successfully employed as an effective antimicrobial agent. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Dananjaya S.H.S.,GODAHEWAGELSHAN IMARSHANA,이영득,조종기,이제희,디조이사마하나마 한국임상수의학회 2014 한국임상수의학회지 Vol.31 No.6
Recently nano particles have proven for wide array of bioactive properties. In the present study, antibacterialproperties of chitosan silver nano composites (CAgNCs) were investigated against fish pathogenic Edwardsiella tarda. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of CAgNCs against E. tarda were 25 μg/mL and 125 μg/mL, respectively. The field emission scanning electron microscope (FE-SEM) imageof CAgNCs treated E. tarda showed the strongly damaged bacteria cells than non-treated bacteria. Furthermore,treatment of CAgNCs induced the level of intracellular reactive oxygen species (ROS) in E. tarda cells in concentrationand time dependent manner suggesting that it may generate oxidative stress leading to bacterial cell death. In addition,MTT assay results showed that the lowest cell viability at 100 μg/mL of CAgNCs treated E. tarda. Overall resultsof this study suggest that CAgNCs is a potential antibacterial agent to control pathogenic bacteria.