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Da Seul KONG,Changman KIM,Eun Joo PACK,Jung Rae KIM 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
1,3-Propandiol is widely used as adhesives, laminates and food additives for three carbon sources. Sustainable and non-toxic regeneration of NADH is critical for efficient production of 1,3-PDO. Zero-Valent Iron(ZVI) can provide reducing equivalent for 1,3-PDO synthesis from glycerol as an electronic donor. Klebsiella Pneumaniae has 1,3-PDO production pathway from glycerol, and has been extensively investigated as exoelectrogens. In this study, we attempt to produce 1,3-PDO from un-purified crude glycerol produced from biodiesel process by using an electrochemically active strain, K. pneumoniae L17. The results showed significant increase of 1,3-PDO production using the ZVI. It seems that unidentified component in a crude glycerol may bring synergetic effects for both cell biomass and 1,3-PDO production. These results implicate that ZVI can regulate the bioconversion of electroactive strain such as L17, therefore improve crude glycerol conversion into value-added platform chemicals.
Genetic analysis of endangered species Crested Ibis (Nipponia nippon) microsatellite markers
Da Hye Kim(김다혜),Yi Seul Kim(김이슬),Joo Hee Seo(서주희),Sung Jin Kim(김성진),Hong Sik Kong(공홍식) 한국조류학회II 2018 한국조류학회지 Vol.25 No.2
세계적으로 멸종위기종인 따오기 (Nipponia Nippon)는 한국에서도 멸종위기종으로 구분되어 있으며, 이를 복원하기 위해 2008년 10월에 중국에서 따오기 1쌍을 도입하여 한국 최초로 인공번식에 성공하였다. 이 후 우포늪 따오기 종 복원을 통해 2017년까지 개체수가 200마리 이상으로 늘어났다. 본 연구에서는 우포늪 따오기 228 개체를 대상으로 성별을 결정하고, microsatellite 마커를 이용하여 유전적 유연관계를 분석하였다. 그 결과, 115마리의 암컷과 113마리의 수컷으로 판별되었으며, 2016년보다 2017년에 서식하고 있는 개체의 이형접합도와 다형성정보지수가 모두 감소한 것으로 나타났다. 이는 작은 집단으로부터 개체 수를 늘려나가다 보니 2017년에 근친율이 증가한 것으로 사료된다. 향 후 본 연구는 한국 따오기의 번식사업 및 복원사업을 위한 기초자료로 유용하게 활용될 것으로 기대된다. The Korean Crested ibis Nipponia Nippon is an endangered species. A pair of Crested ibis was introduced from China in October 2008, and a successful program of artificial incubation of the species, and over 200 animals have been successfully bred through the restoration project up to 2017 at Upo ibis restoration center. We assessed genetic diversity and sex determination in the Korean Crested ibis. In total, 228 Crested ibis (115 females and 113 males) were identified. And genetic diversity measures, observed heterozygosity, expected heterozygosity, and polymorphic information content values were lower in 2017 than those in 2016. The inbreeding coefficient showed that the degree of ancestry increased in 2017. The decrease in polymorphism and increase in the degree of ancestry is thought to be due to inbreeding in such a small group. In this study provided important insight into protocols for genetic management of the breeding population of Korean Crested ibis in Korea and will help in extending the restoration program.