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Cui, Lei,Wu, Song-quan,Zhao, Cheng-ai,Yin, Cheng-ri The Korean Society of Ginseng 2016 Journal of Ginseng Research Vol.40 No.4
Background: In this study, we screened and identified an endophyte JG09 having strong biocatalytic activity for ginsenosides from Platycodon grandiflorum, converted ginseng total saponins and ginsenoside monomers, determined the source of minor ginsenosides and the transformation pathways, and calculated the maximum production of minor ginsenosides for the conversion of ginsenoside Rb1 to assess the transformation activity of endophyte JG09. Methods: The transformation of ginseng total saponins and ginsenoside monomers Rb1, Rb2, Rc, Rd, Rg1 into minor ginsenosides F2, C-K and Rh1 using endophyte JG09 isolated by an organizational separation method and Esculin-R2A agar assay, as well as the identification of transformed products via TLC and HPLC, were evaluated. Endophyte JG09 was identified through DNA sequencing and phylogenetic analysis. Results: A total of 32 ${\beta}$-glucosidase-producing endophytes were screened out among the isolated 69 endophytes from P. grandiflorum. An endophyte bacteria JG09 identified as Luteibacter sp. effectively converted protopanaxadiol-type ginsenosides Rb1, Rb2, Rc, Rd into minor ginsenosides F2 and C-K, and converted protopanaxatriol-type ginsenoside Rg1 into minor ginsenoside Rh1. The transformation pathways of major ginsenosides by endophyte JG09 were as follows: $Rb1{\rightarrow}Rd{\rightarrow}F2{\rightarrow}C-K$; $Rb2{\rightarrow}C-O{\rightarrow}C-Y{\rightarrow}C-K$; $Rc{\rightarrow}C-Mc1{\rightarrow}C-Mc{\rightarrow}C-K$; $Rg1{\rightarrow}Rh1$. The maximum production rate of ginsenosides F2 and C-K reached 94.53% and 66.34%, respectively. Conclusion: This is the first report about conversion of major ginsenosides into minor ginsenosides by fermentation with P. grandiflorum endophytes. The results of the study indicate endophyte JG09 would be a potential microbial source for obtaining minor ginsenosides.
Overexpression of the AtSTK Gene Increases Salt, PEG and ABA Tolerance in Arabidopsis
Lei Bing,Cui-Cui Feng,Jing-Lan Li,Xiao-Xu Li,Baocun Zhao,Yin-Zhu Shen,Zhan-Jing Huang,Rong-Chao Ge 한국식물학회 2013 Journal of Plant Biology Vol.56 No.6
AtSTK (At5g02800), which is a serine-threonineprotein kinase gene of Arabidopsis thaliana, was cloned, andits function was studied. The study found that the overexpressionof AtSTK could significantly improve the ability of A. thaliana to tolerate salt, PEG, and ABA stresses. RT-PCRanalysis revealed that the expression of the AtSTK genecould be obviously induced by salt, PEG, and ABA. Theexamination of the physiological characteristics showed thatthe overexpression of AtSTK in Arabidopsis significantlyreduced the plasma membrane permeability, significantlyincreased the proline content, and decreased the MDA content. These changes may reflect the physiological mechanismsthrough which AtSTK overexpression improves stress resistancein Arabidopsis. In addition, the overexpression of the AtSTKgene significantly antagonised the inhibitory effect of highconcentrations of exogenous ABA on Arabidopsis seedgermination. The subcellular localisation results showed thatAtSTK is located in both the cytosol and the nucleus. Theexamination of its tissue-specific expression showed thatAtSTK is expressed in various Arabidopsis tissues and isparticularly strongly expressed in the vessels. The signallingpathway analysis indicated that AtSTK might transfer thesalt stress signal in Arabidopsis through the MAPK pathway.
Behavior Logic of Burmese Army Taking over Power in 2021——Based on Cost-Benefit Analysis
Cui Lei,Wang Miao 아시아사회과학학회 2022 Jornal of Asia Social Science Vol.7 No.3
Since Myanmar started the process of transforming its political system, the country has progressively developed a political structure of dual power centers (Diarchy), with the NLD and the military taking turns holding the reins of power. Both sides are engaged in what might be described as a a military-political game, and fight without breaking. However, on February 1, 2021, when the new federal parliament of Myanmar conducted its first meeting, the Myanmar military decided to take over the rule, and the country entered a one-year state of emergency, known as the "February 1" Incident. As a result of this action by Myanmar’s military, a political environment that was stable has been disrupted. The causes are discussed in academic circles. There are objective reasons that the development and changes of political environment at home and abroad have greatly reduced the political space of the Burmese army, and there are subjective reasons that Min Onlai’s personal political ambition or his fear of war crimes trial after retirement should not be underestimated. This instance of political power seizure has been researched by scholars. there is no dearth of explanatory power, yet there are still some flaws. Therefore, this paper based on a cost-benefit analysis conducted from the viewpoint of the Burmese military, that the cost of taking over the regime is less than its benefit. Therefore, when the net income is positive, there is a high probability of taking over the regime.
CHISELED NICKEL HYDROXIDE NANOPLATES GROWTH ON GRAPHENE SHEETS FOR LITHIUM ION BATTERIES
LEI-LEI TIAN,XIAN-YONG WEI,QUAN-CHAO ZHUANG,CHAO WU,RUI-LUN XIE,ZHI-MIN ZONG,YONG-LI CUI,SHI-GANG SUN 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2013 NANO Vol.8 No.6
The morphologies and structures of Ni(OH)2–graphene hybrid materials were tailored by using different mineralizers in this work. It was revealed that the synergic effects of the highly oxidized graphene sheets and the mineralizers played a crucial role in controlling the morphology and structure of the nanocomposites, and Na2CO3 is a very effective mineralizer for growing chiseled 2D nanoplates of Ni(OH)2 on graphene sheets. When produced with NaOH, fragmental Ni(OH)2 crystals with irregular shapes erratically decorated on graphene sheets. In contrast, chiseled Ni(OH)2 hexagonal nanoplates grown on graphene sheets were obtained when Na2CO3 was used as the mineralizer. These unique 2D–2D nanoarchitectures with higher contact area between the nanocrystals and graphene substrate can increase the interfacial interaction and then efficiently improve the structural stability of the composite material, thus exhibiting an enhanced Li storage capacity and excellent cycling performance of 562 mAh g-1 after the 36th cycle.
모래상자 수리모형실험을 통한 경계면 모델의 관정 염도 모의실험
시뢰 ( Lei Shi ),최뢰 ( Lei Cui ),이찬종 ( Chan Jong Lee ),홍성훈 ( Sung Hoon Hong ),박남식 ( Nam Sik Park ) 대한지질공학회 2009 지질공학 Vol.19 No.1
본 연구에서는 해안지역 대수층에서 담수와 해수의 흐름을 개략적으로 모의하는 경계면(sharp-interface)모델이 관정규모의 지하수 흐름 문제에도 적용될 수 있는 지 조사하였다. 해안 지역에서 지하수를 개발할 때 고려해야하는 중요한 인자 중의 하나는 해수침투이다. 관정규모의 지하수흐름 문제에서 해수침투 여부를 평가하기 위해서는 양수관정의 염분도를 모의할 수 있어야한다. 경계면 모델의 양수관정 염도 모의 능력을 검증하기 위하여 소규모 모래상자 수리모형실험을 수행하고 경계면모델의 모의능력을 평가하였다. 여러 가지 실험에서 측정된 관정의 염분도는 0%에서 12%의 범위를 보였으며 모의된 염분도는 측정치를 잘 나타내었다. In this work applicability of a sharp-interface model to well-scale problems is examined. In coastal areas one of the main concerns in groundwater development is saltwater intrusion. To determine the saltwater intrusion at a well one needs to evaluate the saltwater content from a pumpin g well. We conducted laboratory sand-tank experiments to determine if a sharp-interface model can simulate the saltwater content of a pumping well. Lateral saltwater intrusion experiments and freshwater lens experiments are conducted. A partially penetrating well and a fully penetrating well are used. Comparison between numerical results and observation results indicates that the sharp-interface model can determine saltwater contents reasonably well. We conclude that a sharp-interface model can be applied to well-scale groundwater flow problems in coastal areas.