Melatonin protects endothelial progenitor L255cells against AGE-induced apoptosis via autophagy flux stimulation and promotes wound healing in diabetic mice

Haiming Jin,Zengjie Zhang,Chengui Wang,Qian Tang,Jianle Wang,Xueqin Bai,Qingqing Wang,Majid Nisar,Naifeng Tian,Quan Wang,Cong Mao,Xiaolei Zhang,Xiangyang Wang 생화학분자생물학회 2018 Experimental and molecular medicine Vol.50 No.-

Wound healing is delayed in diabetic patients. Increased apoptosis and endothelial progenitor cell (EPC) dysfunction are implicated in delayed diabetic wound healing. Melatonin, a major secretory product of the pineal gland, promotes diabetic wound healing; however, its mechanism of action remains unclear. Here, EPCs were isolated from the bone marrow of mice. Treatment of EPCs with melatonin alleviated advanced glycation end product (AGE)-induced apoptosis and cellular dysfunction. We further examined autophagy flux after melatonin treatment and found increased light chain 3 (LC3) and p62 protein levels in AGE-treated EPCs. However, lysosome-associated membrane protein 2 expression was decreased, indicating that autophagy flux was impaired in EPCs treated with AGEs. We then evaluated autophagy flux after melatonin treatment and found that melatonin increased the LC3 levels, but attenuated the accumulation of p62, suggesting a stimulatory effect of melatonin on autophagy flux. Blockage of autophagy flux by chloroquine partially abolished the protective effects of melatonin, indicating that autophagy flux is involved in the protective effects of melatonin. Furthermore, we found that the AMPK/mTOR signaling pathway is involved in autophagy flux stimulation by melatonin. An in vivo study also illustrated that melatonin treatment ameliorated impaired wound healing in a streptozotocin-induced diabetic wound healing model. Thus, our study shows that melatonin protects EPCs against apoptosis and dysfunction via autophagy flux stimulation and ameliorates impaired wound healing in vivo, providing insight into its mechanism of action in diabetic wound healing.

Association of the XRCC1 c.1178G>A Genetic Polymorphism with Lung Cancer Risk in Chinese

Wang, Lei,Lin, Yong,Qi, Cong-Cong,Sheng, Bao-Wei,Fu, Tian Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.9

The X-ray repair cross-complementing group 1 protein (XRCC1) plays important roles in the DNA base excision repair pathway which may influence the development of lung cancer. This study aimed to evaluate the potential association of the XRCC1 c.1178G>A genetic polymorphism with lung cancer risk. The created restriction site-polymerase chain reaction (CRS-PCR) and DNA sequencing methods were utilized to evaluate the XRCC1 c.1178G>A genetic polymorphism among 376 lung cancer patients and 379 controls. Associations between the genetic polymorphism and lung cancer risk were determined with an unconditional logistic regression model. Our data suggested that the distribution of allele and genotype in lung cancer patients was significantly different from that of controls. The XRCC1 c.1178G>A genetic polymorphism was associated with an increased risk of lung cancer (AA vs GG: OR=2.91, 95%CI 1.70-4.98, p<0.001; A vs G: OR=1.52, 95%CI 1.22-1.90, p<0.001). The allele A and genotype AA may contribute to risk of lung cancer. These preliminary results suggested that the XRCC1 c.1178G>A genetic polymorphism is statistically associated with lung cancer risk in the Chinese population.

Study of the color features of the traditional clothing of the Tibet Autonomous Region of China

( Cong Wang ),( Youngjoo Na ) 한국감성과학회 2017 한국감성과학회 국제학술대회(ICES) Vol.2017 No.-

China is a multiracial nation consisting of the Han majority and 55 ethnic minorities, each of which possesses unique cultural characteristics. Among them, the Tibetan Plateau, Sichuan province, Yunnan Province and the current Chinese Tibet Autonomous Region have a long history, traditional culture and a wide range of natural environments. As an example, the Hollywood blockbuster <2012> that shows "the fall of mankind” has received a lot of criticism as the United States acknowledges the domination of the Tibet. However, people are reminded of the Tibet at the end when it becomes the last area with survivors. Due to these characteristics, Tibetan politics hold an important place in China's politics, society, economics and culture. The study uses empirical research methods by analyzing the literature of the Tibetan people. The study of their literature helps to understand the culture of Tibet and to interpret the meaning behind the uses of different colors. The empirical study uses pictures of Tibetan traditional clothes. A total of 85 photos were used for the study of the color palette, which belonged to the Google Art & Design Museum, the Museum of Photography, and the Photo-Friendly Exhibition Hall. By analyzing the results, we can see the use of all the hue-tone colors. Among them, red, orange, yellow, green, indigo, and purple are more likely to be used than other colors. From all the hues, it is easily appreciated that the V hues were the most used. In addition, many color applications were used as well. These different senses of color represent different religions, whose different characteristics appear also on the traditional clothes. Red represents the color of fire and is regarded as a symbol of power. When thinking about red flags in Tibet, they think of the dragon as a symbolic color. Amber also represents the Buddhist temple of Buddhism. Dark blue symbolizes the eldest son, or Chang Cheong, being the most frequently worn color of the Tibetan region. Dark blue represents mystery and the plateau, with the color of blue skies and lakes. Green is the most common color, meaning vitality in the color of grass. Green color is closer to the public, and it is more frequent in a wide range of nomadic areas. Gold and silver represent wealth and dignity, and clothes with those colors represent the wealth and riches of the family. Since white symbolizes life and luck, it is one of the most frequently used colors. Black is one of the most sophisticated colors in people's lives. Most of the great men and women are inseparably related. There are black tents used in the homes of nomads, the black border of Tibet, and a pair of black cranes made by men and women in rural areas.

Optimization Design of an Ultra-Low Specific-Speed Centrifugal Pump Using Entropy Production Minimization and Taguchi Method

Cong Wang,Yongxue Zhang,Hucan Hou,Zhiyi Yuan,Ming Liu 한국유체기계학회 2020 International journal of fluid machinery and syste Vol.13 No.1

To improve the energy conversion efficiency and cavitation performance of the ultra-low specific-speed centrifugal pump (ULSSCP), the impeller-volute interaction has been studied. Blade outlet setting angle (β₂), wrap angle (φ), volute inlet width (b₃) and throat area (St) were addressed as the design parameters. The entropy production at 0.5 Q0 and NPSHc (critical net positive suction head) at 1.5 Q0 were selected as the target to characterize the energy loss and cavitation performance. The L9 (3⁴) orthogonal matrix was established via Taguchi method. Results show that the contribution ratio of φ on S/NS is the most vital, followed by b₃ and St, while the influence of β₂ is relatively small, and the design parameter combination with β₂=19°, φ=220°, b₃=12mm and St=190㎟ is the best choice for the lowest entropy production at 0.5Q0 and NPSHc. At last, the optimization design reduces the loss greatly before and after cavitation by alleviation of vortex generation and backflow intensity.

A network meta-analysis of the short-term efficacy of five chemotherapy regimens based on cisplatin and fluorouracil for esophagogastric junctional adenocarcinoma

Cong Wang,Dong-Jian Song,Zhi-Li Xu,Shu-Ping Xie,Jun-Hong Hu 생화학분자생물학회 2017 Experimental and molecular medicine Vol.49 No.-

The primary purpose of this study was to explore the short-term efficacy of different cisplatin and fluorouracil-based chemotherapy regimens in the treatment of patients with esophagogastric junctional adenocarcinoma (EGJA) using a network meta-analysis (NMA). Randomized controlled trials (RCTs) related to chemotherapy regimens based on cisplatin and fluorouracil for EGJA were included from the PubMed, EMBASE and Cochrane Library electronic databases (from inception to June 2016). Direct and indirect evidence were combined to calculate the pooled odds ratio (OR) and its 95% confidence interval (95% CI) as well as to draw the surface under the cumulative ranking (SUCRA) curves. This NMA finally enrolled ten eligible RCTs with the following five regimens: cisplatin plus fluorouracil (cisplatin+fluorouracil), cisplatin+fluorouracil-based chemotherapy (cisplatin+fluorouracil+docetaxel/epirubicin/irinotecan), fluorouracil-based chemotherapy (fluorouracil+docetaxel/doxorubicin/ methotrexate/irinotecan), cisplatin-based chemotherapy (cisplatin+docetaxel/epirubicin/irinotecan/capecitabine/s-1) and other drug-based chemotherapy (docetaxel/irinotecan/capecitabine). These results revealed that compared with a cisplatin+ fluorouracil-based chemotherapy regimen, the fluorouracil-based chemotherapy regimen had a lower overall response rate (ORR) and partial response (PR) for EGJA patients (ORR: OR=0.43, 95% CI=0.22–0.86; PR: OR=0.46, 95% CI=0.23–0.91). Cluster analyses suggested that the cisplatin+fluorouracil-based chemotherapy regimen had the best short-term efficacy for EGJA in terms of the complete response (CR), PR, ORR, stable disease (SD) and progression disease (PD). Our results indicated that cisplatin+fluorouracil-based chemotherapy regimens may have the best short-term efficacy in the treatment of EGJA

Application and Simulation of Distribution Function in Grape Drying Process

Cong Wang 보안공학연구지원센터 2016 International Journal of u- and e- Service, Scienc Vol.9 No.10

In order to investigate the influence factors of Weibull distribution function and its application in drying, the drying process of grape slices in different microwave vacuum drying power (330, 460, 590W) and vacuum degree (-50, -70, -90kPa) was taken as the research object, and the Weibull distribution function was used to simulate and analyze its drying kinetic curves, the results showed that the Weibull distribution function was able to simulate microwave vacuum drying process of grape slice well: scaling parameterαwas related with microwave power and vacuum degree, and decreased with the rise of them; the influence of drying variable on the form parameterβwas relatively low. The estimated effective moisture diffusivity coefficient in the dry process was obtained by calculating, its value increased with the rise of microwave power and vacuum degree in the scope from 3.45078×10-7 to 6.74613×10-7m2/s; through Arrhenius equation, the activation energy of drying was calculated respectively 1.34701, 1.49099 and 1.57108 W/g under the vacuum degree of -50, -70 and -90kPa. This study has provided the technical basis for the application of Weibull distribution function in microwave vacuum drying technology of grape slices.

Design of High-performance Wilkinson Power Divider on SI-GaAs Substrate using Integrated Passive Technology

Wang, Cong,Kim, Nam-Young Taylor Francis 2012 IETE journal of research Vol.58 No.4

PREPARATION AND CHARACTERIZATION OF SURFACE-FUNCTIONALIZATION OF SILICA-COATED MAGNETITE NANOPARTICLES FOR DRUG DELIVERY

CONG-WANG ZHANG,CHANG-CHUN ZENG,YING XU 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2014 NANO Vol.9 No.4

Fe 3 O 4 – SiO 2 core – shell structure nanoparticles containing magnetic properties were investigatedfor their potential use in drug delivery. The Fe 3 O 4 – SiO 2 core – shell structure nanoparticles weresuccessfully synthesized by a simple and convenient way. The Fe 3 O 4 – SiO 2 nanoparticles showedsuperparamagnetic behavior, indicating a great application potential in separation technologies. From the application point of view, the prepared nanoparticles were found to act as an e±cientdrug carrier. Speci¯cally, the surface of the core – shell nanoparticles was modi¯ed with aminogroups by use of silane coupling agent 3-aminopropyltriethoxysilane (APTS). Doxorubicin(DOX) was successfully grafted to the surface of the core – shell nanoparticles after the decorationwith the carboxyl acid groups on the surface of amino-modi¯ed core – shell structure nano-particles. Moreover, the nanocomposite showed a good drug delivery performance in the DOX-loading e±ciency and drug release experiments, con¯rming that the materials had a great ap-plication potential in drug delivery. It is envisioned that the prepared materials are the idealagent for application in medical diagnosis and therapy.

Adaptive robust target tracking control of marching tank under high-speed maneuvering condition

Cong Li,Guolai Yang,Xiuye Wang,Yuze Ma,Liqun Wang,Quanzhao Sun 대한기계학회 2022 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.36 No.6

This paper focuses on the problem of target tracking control of marching tanks under the condition of high-speed maneuver based on sliding mode disturbance observer. First, the dynamic model of marching tank is established by using multi-body dynamics software. The nonlinear factors such as barrel flexibility, clearance between barrel and bushing, and uncertainty sources such as road excitation are considered. Second, the mechatronics model of tank bidirectional stabilization system considering transmission clearance and friction is established in numerical simulation software. Aiming at the tank bidirectional stabilization system, an adaptive robust controller based on sliding mode disturbance observer is designed. Finally, the real-time control and target tracking of the tank bidirectional stabilization system are realized by co-simulation. The results show that the sliding mode disturbance observer improves the stability accuracy of the system by 43.22 %. Comparing the simulation results with the PID control, the controller designed in this paper has the characteristics of rapid tracking speed and high robustness, and the advantage is more obvious under the condition of highspeed maneuver.

High Performance Wilkinson Power Divider Using Integrated Passive Technology on SI-GaAs Substrate

Cong Wang,Cheng Qian,De-Zhong Li,Wen-Cheng Huang,Nam-Young Kim 한국전자파학회JEES 2008 Journal of Electromagnetic Engineering and Science Vol.8 No.3

An integrated passive device(IPD) technology by semi-insulating(SI)-GaAs-based fabrication has been developed to meet the ever increasing needs of size and cost reduction in wireless applications. This technology includes reliable NiCr thin film resistor, thick plated Cu/Au metal process to reduce resistive loss, high breakdown voltage metalinsulator-metal(MIM) capacitor due to a thinner dielectric thickness, lowest parasitic effect by multi air-bridged metal layers, air-bridges for inductor underpass and capacitor pick-up, and low chip cost by only 6 process layers. This paper presents the Wilkinson power divider with excellent performance for digital cellular system(DCS). The insertion loss of this power divider is ?0.43 ㏈ and the port isolation greater than ?22 ㏈ over the entire band. Return loss in input and output ports are ?23.4 ㏈ and ?25.4 ㏈, respectively. The Wilkinson power divider based on SI-GaAs substrates is designed within die size of 1.42 ㎟.