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Cervid satellite DNA and karyotypic evolution of Indian muntjac
Yueh-Chun Li,Chyi-Chyang Lin 한국유전학회 2012 Genes & Genomics Vol.34 No.1
Five satellite DNA families (designated as satellite I-V) have been identified in the Cervidae so far. Among those, satellite I, II and IV are centromere specific. Satellite I and II are shared by large number of deer species, where satellite IV is highly conserved among several deer species examined. Satellite III was initially thought to be roe deer specific but later identified in Chinese water deer as well. SatelliteV is Y-chromosome specific for several Asian deer species examined but also found in the pericentric region of Indian muntjac chromosome 3 and in X chromosome of Chinese water deer. The observation of interstitial hybridization sites on Indian muntjac chromosomes with satellite DNA I probe generated from Chinese muntjac provides the first molecular evidence supporting the tandem fusion theory that 2n=6♀/7♂of Indian muntjac karyotype could derive from an ancestral Chinese muntjac-like species with 2n=46. Interspecies chromosome painting study and the maximum number of interstitial hybridization detected with satellite I and satellite II DNA probes lend support to the hypothesis that the Indian muntjac karyotype could evolve directly from an ancestral Chinese water deer-like species with 2n=70. Such hypothesis is further substantiated by the finding of satellite V signals presented in specific chromosome regions between the Chinese water deer and the Indian muntjac chromosomes.
The Effect of Swirling Flow on Elutriation in a Vortexing Fluidized Bed
Wan, Hou Peng,Chyang, Chien Song 한국화학공학회 1999 Korean Journal of Chemical Engineering Vol.16 No.5
The elutriation of fine particles in a vortexing fluidized bed (VFB) was studied by a batch and binary system. The diameter of the coarse particles was 545㎛, and the diameter of the fine particles for the elutriation test was 81, 97, 115, 137, 163, and 193㎛, respectively. It was found that the swirling flow caused by the secondary air injection is the dominating factor to influence the elutriation rates. The effect of primary air velocity, swirling flow, injection angle of secondary air nozzle, and diameter of fine particles on the elutriation rate constant was also studied. The Taguchi experimental method and Regular analysis are used to identify the effects of various operating variables. A correlation was developed to estimate the specific elutriation rate constant (K^*_∞) in the vortexing fluidized bed. The specific elutriation rate constant (K^*_∞) was found to be a function of the primary air velocity, the diameter of fine particles, the secondary air velocity, and the height of secondary air injection.
The Simulation Study of Circulating Tumor Cells Collection using Electrophoresis Method
( Guo-hao Lu ),( Deng-chyang Wu ),( Chao-wang Young ) 한국농업기계학회 2018 한국농업기계학회 학술발표논문집 Vol.23 No.1
Cancer is one of the leading causes of morbidity and mortality worldwide. The most method in the treatment of cancer is early detection, and the concentration of circulating tumor cells (CTCs) in the peripheral blood is the important factor. CTCs are cells that have shed into the vasculature or lymphatic from a primary cancer cells and are carried around the body in the circulation system. CTCs thus constitute seeds for the subsequent growth of additional tumors in vital distant organs, triggering a mechanism that is responsible for the vast majority of cancer-related deaths. CTCs also have the potential to provide a mechanism for early patient prognoses and to determine appropriate tailored treatments. In this study, the technologies of electrophoresis and micro-flow channel were used to separate two different size (20μm, 10μm) particles (simulate as CTCs and normal cells), for collecting sufficient concentration of CTCs from the patient peripheral blood. Results show that this electrophoresis method can separate effectually two different size particles, under the field of dielectric electrophoresis (1MHz, 10V<sub>pp</sub>),theinjectionflow(0.5μl/min) and solution conductivity (430μs/cm) in self-made micro-flow channel.
Wei-Hong Liang,Tung-Wu Chang,Yuh-Chyang Charng 한국식품과학회 2018 Food Science and Biotechnology Vol.27 No.4
Baizhi (Angelica dahurica) has been widely used as a traditional Chinese herbal medicine, functional food and cosmetic product ingredient, mostly because of the high furanocoumarin compounds in roots. Because the fresh root is perishable, drying techniques are needed to maintain a higher-quality product. Freeze-drying is the best method but energy-consuming and costly. The aim of this study was to analyze the quality (antioxidant and furanocoumarin content) of Baizhi roots after freeze-drying (the control) and in-the-shade, 40 and 70 C drying. Antioxidant activity was revealed by 2,2-diphenyl-1- picrylhydrazyl and Fe2? chelating assay, and the content of six furanocoumarin compounds, including xanthotoxin, bergapten, oxypeucedanin, imperatorin, phellopterin and isoimperatorin, was analyzed by liquid chromatography. Antioxidant activity was greater in roots with in-the-shade, 40 and 70 C drying than freeze-drying. The furanocoumarin content pattern was similar with 70 C drying and freeze-drying. A. dahurica roots dried at 70 C may be an alternative method for maintaining high quality.
RESEARCH PAPERS : The Effect of Swirling Flow on Elutriation in a Vortexing Fluidized Bed
(Hou Peng Wan),(Chien Song Chyang) 한국화학공학회 1999 Korean Journal of Chemical Engineering Vol.16 No.5
The elutriation of fine particles in a vortexing fluidized bed (VFB) was studied by a batch and binary system. The diameter of the coarse particles was 545㎛, and the diameter of the fine particles for the elutriation test was 81, 97, 115, 137, 163, and 193㎛, respectively. It was found that the swirling flow caused by the secondary air injection is the dominating factor to influence the elutriation rates. The effect of primary air velocity, swirling flow, injection angle of secondary air nozzle, and diameter of fine particles on the elutriation rate constant was also studied. The Taguchi experimental method and Regular analysis are used to identify the effects of various operating variables. A correlation was developed to estimate the specific elutriation rate constant (K^*_∞) in the vortexing fluidized bed. The specific elutriation rate constant (K^*_∞) was found to be a function of the primary air velocity, the diameter of fine particles, the secondary air velocity, and the height of secondary air injection.