http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : Chuanmei Xu (검색결과 3 건)
- 무료
- 기관 내 무료
- 유료
-
Qin, Qiaoping,Zhang, Lanlan,Xu, Kai,Jiang, Li,Cheng, Longjun,Xu, Chuanmei,Cui, Yongyi Korean Society of Horticultural Science 2012 원예과학기술지 Vol.30 No.3
Hexokinase is the first enzyme in the hexose assimilation pathway; it acts as a sensor for plant sugar responses, and it is also important in determining the fruit sugar levels. The full-length cDNA of a hexokinase gene was isolated from loquat through reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends, which was designated as EjHXK1. EjHXK1 is 1,839 bp long and contains an entire open reading frame encoding 497 amino acids. The predicted protein of EjHXK1 shares 72%-81% similarity with other plant hexokinases. Phylogeny analysis indicated that EjHXK1 is closely related to maize and rice hexokinases. Transient expression of the 35S: EjHXK1-GFP fusion protein was observed on the cell membrane and cytoplasm. Real-time RT-PCR indicated that EjHXK1 is expressed in loquat leaves, stems, flowers, and fruits. EjHXK1 transcripts were higher during early fruit development, but decreases before maturation, which is consistent with hexokinase enzyme activity during fruit development and conducive for hexose accumulation in mature fruits. These results imply that EjHXK1 may play important roles in the regulation of sugar flux during fruit ripening.
-
Qiaoping Qin,Lanlan Zhang,Kai Xu,Li Jiang,Longjun Cheng,Chuanmei Xu,Yongyi Cui 한국원예학회 2012 원예과학기술지 Vol.30 No.3
Hexokinase is the first enzyme in the hexose assimilation pathway; it acts as a sensor for plant sugar responses, and it is also important in determining the fruit sugar levels. The full-length cDNA of a hexokinase gene was isolated from loquat through reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends, which was designated as EjHXK1. EjHXK1 is 1,839 bp long and contains an entire open reading frame encoding 497 amino acids. The predicted protein of EjHXK1 shares 72%-81% similarity with other plant hexokinases. Phylogeny analysis indicated that EjHXK1 is closely related to maize and rice hexokinases. Transient expression of the 35S: EjHXK1-GFP fusion protein was observed on the cell membrane and cytoplasm. Real-time RT-PCR indicated that EjHXK1 is expressed in loquat leaves, stems, flowers, and fruits. EjHXK1 transcripts were higher during early fruit development, but decreases before maturation, which is consistent with hexokinase enzyme activity during fruit development and conducive for hexose accumulation in mature fruits. These results imply that EjHXK1 may play important roles in the regulation of sugar flux during fruit ripening.
-
Correlation analysis between the karyotypes and phenotypic traits of Chinese cymbidium cultivars
Huijuan Ning,Suyan Ao,Yirong Fan,Jianxin Fu,Chuanmei Xu 한국원예학회 2018 Horticulture, Environment, and Biotechnology Vol.59 No.1
Chinese cymbidium are a group of valuable ornamental plants that is formed by genetically distinct species. Currently, arobust Chinese cymbidium classification system, such as that based on cytotaxonomy, is lacking. To investigate the geneticvariation within Chinese cymbidium, we selected 28 individual Chinese cymbidium cultivars as experimental materialto perform karyotype analysis. The results showed that chromosome number among these 28 cultivars was constant anddiploid (i.e., 2n = 40). In addition, no satellite chromosomes were observed. The karyotypes were more symmetrical andconsisted mainly of 1B and 2B types with a small number of 2A and 2C types. Based on principal component analysis, thefive karyotype parameters with the highest classification values were screened, which revealed that the mean of long to shortarm ratio, the mean relative length of long arms, and the asymmetry coefficient of karyotypes (As.K.) were reliable parametersfor use in Chinese cymbidium cytotaxonomy. Cluster analysis of karyotype parameters successfully identified cultivarsof the bamboo petal, narcissus petal, and plum petal types, thus confirming the accuracy of the cytological classificationsystem. Furthermore, flower traits of Chinese cymbidium were strongly correlated with karyotype parameters. Overall, weconclude that karyotype parameters can provide valuable information for studies on the classification, identification, andgenetic diversity of Chinese cymbidium.
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
