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RISS 인기검색어
- 검색키워드
- 저자 : Che Wu-nan (검색결과 2 건)
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Prevalence of Fumonisin Contamination in Corn and Corn-based Feeds in Taiwan
Cheng, Yeong-Hsiang,Wu, Jih-Fang,Lee, Der-Nan,Yang, Che-Ming J. Asian Australasian Association of Animal Productio 2002 Animal Bioscience Vol.15 No.4
The purpose of this study was to investigate the prevalence of fumonisin contamination in corn and corn-based feeds in Taiwan. A total of 233 samples was collected from 8 feed mill factories located in four different regions in Taiwan. The presence of fumonisin $B_1$ ($FB_1$) and $B_2$ ($FB_2$) was determined by thin layer chromatograph, while the total fumonisin content was determined using immuno-affinity column cleanup and fluorometer quantitation. Our results showed that 55 samples of swine feeds had the highest percentage of incidence of $FB_1$ and $FB_2$ (41.8% and 41.8%, respectively), followed by 66 samples of duck feeds (40.9% and 37.8%). However, the percentage of incidence of $FB_1$ and $FB_2$ was much lower in 43 samples of broiler feeds (23.2% and 13.9%) and 69 samples of corn (17.3% and 10.1%). Corn and duck feeds were found to have a significant higher level of means of total fumonisins ($5.4{\pm}1.5$ and $5.8{\pm}0.6$ ppm, respectively) than swine feeds ($2.9{\pm}0.4$ ppm) and broiler feeds ($3.0{\pm}0.5$ ppm). Comparing fumonisins distribution in different regions, the highest percentage of $FB_1$ incidence (39.2%) was found in the eastern region of Taiwan, and total fumonisins level ($4.5{\pm}0.7$ ppm) was significantly higher than other regions. However, the highest percentage of $FB_2$ incidence (32.0%) was found in the central region of Taiwan. Trimonthly analysis of data showed that both high percentage of $FB_1$ and $FB_2$ incidence (39.3% and 37.7%) and total concentration of fumonisin ($5.7{\pm}0.4$ ppm) were found in the period of Jan. to Mar., The incidence and concentration were significantly higher than other trimothly periods. These results indicate that fumonisin B mycotoxins are both widespread and persistent in feed-grade corn and corn-based feeds in Taiwan.
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Huo Liang-xiao,Bai Xue-ping,Che Wu-nan,Ning Su-fang,Lv Lin,Zhang Lisheng,Zhou Jin-cheng,Dong Hui 한국응용곤충학회 2022 Journal of Asia-Pacific Entomology Vol.25 No.2
The egg parasitoid, Trichogramma spp., is an important biological control agent used against a broad range of Lepidopteran pests in agriculture and forestry. The biology of Trichogramma has been studied in details. Further studies should focus on the molecular mechanisms of Trichogramma by qualifying the expression of related genes It is critical to select appropriate reference genes for normalizing RT-qPCR results and establishing a robust method for quantifying target gene expression. This study aimed to identify and validate appropriate reference genes for use in RT-qPCR analysis of Trichogramma dendrolimi. Ten candidate housekeeping genes, namely betaactin (ACTIN), forkhead box O (FOXO), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), heat shock pro tein 90 (HSP90), ribosomal protein L10a (RPL10a), L18 (RPL18), L28 (RPL28), S13 (RPS13), S15 (RPS15), and superoxide dismutase (SOD), were tested for their suitability as reference genes for developmental stage (3rd, 4th, 5th, 6th, 7th, 8th, 9th, and 10th day after parasitization), tissue (head, thorax, and abdomen of adults), sex of adults (male and female), and temperature (17℃, 25℃, and 32℃). According to the GeNorm analysis, a robust analysis should involve using an appropriate combination of reference genes, namely, at least three genes for different development stages, two genes for different tissues, two genes for different sex, and two genes for different temperatures, respectively. According to the RelFinder method by the integrated results of GeNorm, NormFinder, BestKeeper, and the ΔCt method, we identified the developmental stage-specific reference genes SOD, GAPDH, and ACTIN; tissue-specific reference genes RPL18 and RPS15; sex-specific reference genes RPL18 and SOD; and temperature-specific reference genes RPL18 and RPL10a. This study provides a standardized procedure for the quantification of gene expression in T. dendrolimi and will be helpful for future biological control programs using Trichogramma wasps.
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