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Aditi Sharma,SeungHwan Lee,JunHeon Lee,Changgwon Dang,Hyeong Cheul Kim,SeongHum Yeon 충남대학교 농업과학연구소 2014 농업과학연구 Vol.41 No.4
The evolutionary history of cattle and buffalo has always been a topic of great interest to the evolutionary biologists. The phylogenetic studies of bovin species has been carried out at various levels, varying from the study of domestication and migration of populations to major cladogenesis. Along with the archeological studies there are studies from molecular biology and more recently from genomics. The phylogenetic perspective of the bovins and their evolutionary history, are reviewed in terms of what has been done, what needs to be done and potential challenges in doing it.
Sharma, Aditi,Lee, SeungHwan,Lee, JunHeon,Dang, Changgwon,Kim, Hyeong Cheul,Yeon, SeongHum,Kang, HeeSeol,Kanwar, Shamsher Singh,Vijh, Ramesh Kumar Institute of Agricultural Science 2014 Korean Journal of Agricultural Science Vol.41 No.4
The evolutionary history of cattle and buffalo has always been a topic of great interest to the evolutionary biologists. The phylogenetic studies of bovin species has been carried out at various levels, varying from the study of domestication and migration of populations to major cladogenesis. Along with the archeological studies there are studies from molecular biology and more recently from genomics. The phylogenetic perspective of the bovins and their evolutionary history, are reviewed in terms of what has been done, what needs to be done and potential challenges in doing it.
Early Pregnancy Diagnosis in Dairy Cattle – a Proteomic Approach
Hyun-Joo Lim,Ho-Beak Yoon,Changgwon Dang 한국수정란이식학회 2016 한국수정란이식학회 학술대회 Vol.2016 No.10
High reproductive efficiency is a prerequisite for dairy animals in order to optimize dairy production. An accurate and early pregnancy diagnosis is a crucial aspect for better reproductive management in livestock. The indigenous, visual and clinical methods have various limitations including accuracy, sensitivity, specificity, later stages of applicability and requires highly skilled manpower. This hurdles the researchers to ensue further research on developing novel early pregnancy diagnostics for dairy animals. However, the advantage of molecular techniques like proteomics has given a new hope to look for pregnancy biomarkers in animal research. In this study, we assayed the pregnancy-associated glycoprotein (PAG) levels using anti-bPAG antibody. Serum plasma samples were collected 30, 60, 90 and 120 days after insemination. Cattle diagnosed pregnant with singleton are used for this experiment in which blood samples were collected. The plasma PAG level is gradually increased after insemination to until 60 days and drastically decreased after that. The PAG level was approximately 2-fold greater during 30 days compared with 60 days. These results concluded to validate our other pregnancy diagnosis methods including circulating microRNA and cell free DNA concentration (data not shown). Despite this proteomic approach, validating all other molecular technique results may give the exact time for early pregnancy diagnosis.
Fetal Sex Determination in Dairy Cattle using DNA Isolated from Maternal Plasma
Hyun-Joo Lim,Hyun Jong Kim,Ji Hwan Lee,Soo Bong Park,Seung Min Ha,Dong Hyun Lim,Tae IL Kim,Min Su Kim,Kyung Suk Lee,Changgwon Dang 한국동물생명공학회(구 한국동물번식학회) 2018 발생공학 국제심포지엄 및 학술대회 Vol.2018 No.06
The development of methodological strategies that allow for the prediction of the fetal sex in cattle still remains a zoo-technical challenge. So far, different methods have been implemented to direct the breeding management in dairy cattle, giving producers an advantage in decision-making regarding the activity of planning and monetary gains. The objective of this study was to evaluate the use of next generation sequencing of fetal DNA in the maternal plasma of pregnant cattle in order to determine the sex of the fetus. The whole blood samples were collected from the five Holstein cows at 20-week of pregnancy. The serum plasma was harvested and the cell free DNA (cfDNA) was isolated in order to construct whole genome DNA library. The resulting DNA libraries were then paired-end sequenced (2×101 bp) with Illumina’s HiSeq 2500 instrument. The high quality, clean reads were mapped to the Bovine reference UMD 3.1 of X and Y chromosome separately, resulting an average depth and coverage of 3.54 and 86.2%. Our results revealed that four dairy cattle with male fetuses and 1 with a female fetus. Furthermore, it was concluded that fetal cells in maternal plasma can be used to predict the sex of the fetus in cattle. The detection of fetal DNA sequences is a reality and could reduce the risk of invasive techniques for certain fetal disorders in the near future.